Restriction map for pF1KA1694
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AspI        AviII       AvrII       BamHI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BlnI        BsaBI       BsiWI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        DraIII      EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HpaI        KpnI        MaeI        
MamI        MluI        MseI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SspBI       SspI        StuI        SwaI        Tru9I       
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII        2296  GACGTC               GACGT^C
AflIII         77  ACGTGT               A^C[GA][CT]GT
AhdI         1135  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1639  GTGCAC               G^TGCAC
AocI          240  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1639  GTGCAC               G^TGCAC
AspEI        1135  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1418  TGGCCA               TGG^CCA
BbeI          106  GGCGCC               GGCGC^C
BbrPI        1207  CACGTG               CAC^GTG
BseRI          49  GAGGAG               GAGGAG(10/8)
BsmFI        1709  GTCCC                GGGAC(10/14)
BsmI          369  GAATGC               GAATGC(1/-1)
BspMI         440  ACCTGC               ACCTGC(4/8)
BsrBI         754  GAGCGG               CCGCTC(-3/-3)
BsrDI         468  GCAATG               GCAATG(2/0)
BstAPI        740  GCAAGCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        240  CCTGAGG              CC^T[ACGT]AGG
DrdI          175  GACATTCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1135  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        240  CCTGAGG              CC^T[ACGT]AGG
EcoRI         730  GAATTC               G^AATTC
HindIII      2272  AAGCTT               A^AGCTT
KasI          106  GGCGCC               G^GCGCC
KspI          202  CCGCGG               CCGC^GG
MfeI          338  CAATTG               C^AATTG
MluNI        1418  TGGCCA               TGG^CCA
MscI         1418  TGGCCA               TGG^CCA
MunI          338  CAATTG               C^AATTG
NarI          106  GGCGCC               GG^CGCC
PflMI        1740  CCAGACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1207  CACGTG               CAC^GTG
PmlI         1207  CACGTG               CAC^GTG
PshAI        2160  GACCCCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         202  CCGCGG               CCGC^GG
SapI          127  GCTCTTC              GCTCTTC(1/4)
SbfI          579  CCTGCAGG             CCTGCA^GG
ScaI          380  AGTACT               AGT^ACT
SfoI          106  GGCGCC               GGC^GCC
SmaI          962  CCCGGG               CCC^GGG
Sse8387I      579  CCTGCAGG             CCTGCA^GG
Van91I       1740  CCAGACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         962  CCCGGG               C^CCGGG
XmaI          962  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccIII        545  TCCGGA               T^CCGGA
             1064  TCCGGA              
ApaI         1335  GGGCCC               GGGCC^C
             1909  GGGCCC              
Asp700I       343  GAAGACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              912  GAAGAAGTTC          
AvaI           18  CTCGGG               C^[CT]CG[GA]G
              962  CCCGGG              
BanI          106  GGCGCC               G^G[CT][GA]CC
              197  GGCACC              
BbsI          343  GAAGAC               GAAGAC(2/6)
             1513  GAAGAC              
BglI         1234  GCCAAGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1911  GCCCACCAGGC         
BglII         720  AGATCT               A^GATCT
             2259  AGATCT              
BlpI         1545  GCTGAGC              GC^T[ACGT]AGC
             1965  GCTGAGC             
Bpu1102I     1545  GCTGAGC              GC^T[ACGT]AGC
             1965  GCTGAGC             
BpuAI         343  GAAGAC               GAAGAC(2/6)
             1513  GAAGAC              
BseAI         545  TCCGGA               T^CCGGA
             1064  TCCGGA              
BsgI         1120  CTGCAC               GTGCAG(16/14)
              799  GTGCAG              
BsiEI        1089  CGACCG               CG[GA][CT]^CG
             1347  CGACCG              
BsoBI          18  CTCGGG               C^[CT]CG[GA]G
              962  CCCGGG              
BspEI         545  TCCGGA               T^CCGGA
             1064  TCCGGA              
BstXI         514  CCAAGCCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              974  CCAACCTGGTGG        
CelII        1545  GCTGAGC              GC^T[ACGT]AGC
             1965  GCTGAGC             
MroI          545  TCCGGA               T^CCGGA
             1064  TCCGGA              
NaeI         1239  GCCGGC               GCC^GGC
             1558  GCCGGC              
NcoI          767  CCATGG               C^CATGG
             1745  CCATGG              
NgoMIV       1239  GCCGGC               G^CCGGC
             1558  GCCGGC              
PleI          589  GACTC                GAGTC(4/5)
             2205  GAGTC               
PpuMI         965  GGGTCCT              [GA]G^G[AT]CC[CT]
             1707  GGGTCCC             
SexAI         977  ACCTGGT              A^CC[AT]GGT
             1136  ACCTGGT             
SfaNI        1767  GATGC                GCATC(5/9)
             1325  GCATC               
SphI          929  GCATGC               GCATG^C
             1715  GCATGC              
XcmI           56  CCAAGCCGCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1127  CCAGCCCGGACCTGG     
XmnI          343  GAAGACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              912  GAAGAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BanII       BbvI        BcnI        BmyI        
BpmI        BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfcI        SmlI        StyI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com