Restriction map for pF1KA1694
 (Linear) (Six-base) MAPSORT of: KIAA1694.seq  Check: 989  from: 1  to: 2319



KIAA1694.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AatII G_ACGT'C

Cuts at:      0    2300    2319 
   Size:       2300      19
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1075    1507    1906    2284    2319 
   Size:       1075     432     399     378      35

  Fragments arranged by size: 

                1075     432     399     378      35
 
AflIII A'CryG_T

Cuts at:      0      77    2319 
   Size:         77    2242
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1140    2319 
   Size:       1140    1179
 
AleI CACnn'nnGTG

Cuts at:      0     793    1837    2319 
   Size:        793    1044     482

  Fragments arranged by size: 

                1044     793     482
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2065    2097    2319 
   Size:       2065      32     222

  Fragments arranged by size: 

                2065     222      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     357    1948    2030    2145    2176    2319 
   Size:        357    1591      82     115      31     143

  Fragments arranged by size: 

                1591     357     143     115      82      31
 
ApaI G_GGCC'C

Cuts at:      0    1339    1913    2319 
   Size:       1339     574     406

  Fragments arranged by size: 

                1339     574     406
 
ApaLI G'TGCA_C

Cuts at:      0    1639    2319 
   Size:       1639     680
 
ApoI r'AATT_y

Cuts at:      0     235     730    1675    2319 
   Size:        235     495     945     644

  Fragments arranged by size: 

                 945     644     495     235
 
AvaI C'yCGr_G

Cuts at:      0      18     962    2319 
   Size:         18     944    1357

  Fragments arranged by size: 

                1357     944      18
 
BanI G'GyrC_C

Cuts at:      0     106     197    2319 
   Size:        106      91    2122

  Fragments arranged by size: 

                2122     106      91
 
BanII G_rGCy'C

Cuts at:      0     129     890    1277    1339    1657    1913    1972    2197 
   Size:        129     761     387      62     318     256      59     225

Cuts at:   2197    2319 
   Size:        122

  Fragments arranged by size: 

                 761     387     318     256     225     129     122      62
                  59
 
BbeI G_GCGC'C

Cuts at:      0     110    2319 
   Size:        110    2209
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     350    1520    2319 
   Size:        350    1170     799

  Fragments arranged by size: 

                1170     799     350
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1240    1917    2319 
   Size:       1240     677     402

  Fragments arranged by size: 

                1240     677     402
 
BglII A'GATC_T

Cuts at:      0     720    2259    2319 
   Size:        720    1539      60

  Fragments arranged by size: 

                1539     720      60
 
BlpI GC'TnA_GC

Cuts at:      0    1546    1966    2319 
   Size:       1546     420     353

  Fragments arranged by size: 

                1546     420     353
 
Bme1580I G_kGCm'C

Cuts at:      0     116     841     950    1339    1643    1913    2319 
   Size:        116     725     109     389     304     270     406

  Fragments arranged by size: 

                 725     406     389     304     270     116     109
 
BmgBI CAC'GTC

Cuts at:      0      78    1839    2319 
   Size:         78    1761     480

  Fragments arranged by size: 

                1761     480      78
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     314    2319 
   Size:        314    2005
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1430    1551    2319 
   Size:       1430     121     768

  Fragments arranged by size: 

                1430     768     121
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1179    1425    1846    2011    2122    2319 
   Size:       1179     246     421     165     111     197

  Fragments arranged by size: 

                1179     421     246     197     165     111
 
Bpu10I CC'TnA_GC

Cuts at:      0    2191    2319 
   Size:       2191     128
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     796    1159    1363    2047    2319 
   Size:        796     363     204     684     272

  Fragments arranged by size: 

                 796     684     363     272     204
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      46     440    1401    2152    2319 
   Size:         46     394     961     751     167

  Fragments arranged by size: 

                 961     751     394     167      46
 
BsaAI yAC'GTr

Cuts at:      0    1209    1368    1494    2319 
   Size:       1209     159     126     825

  Fragments arranged by size: 

                1209     825     159     126
 
BsaHI Gr'CG_yC

Cuts at:      0     107     289    2297    2319 
   Size:        107     182    2008      22

  Fragments arranged by size: 

                2008     182     107      22
 
BsaWI w'CCGG_w

Cuts at:      0     545    1064    1091    2319 
   Size:        545     519      27    1228

  Fragments arranged by size: 

                1228     545     519      27
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     310     340    1744    1774    2319 
   Size:        310      30    1404      30     545

  Fragments arranged by size: 

                1404     545     310      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64    2319 
   Size:         64    2255
 
BseYI C'CCAG_C

Cuts at:      0      66     248     521    1126    1332    1704    2319 
   Size:         66     182     273     605     206     372     615

  Fragments arranged by size: 

                 615     605     372     273     206     182      66
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820    1105    2319 
   Size:        820     285    1214

  Fragments arranged by size: 

                1214     820     285
 
BsiEI CG_ry'CG

Cuts at:      0    1092    1350    2319 
   Size:       1092     258     969

  Fragments arranged by size: 

                1092     969     258
 
BsiHKAI G_wGCw'C

Cuts at:      0     890    1643    1834    1972    2042    2197    2319 
   Size:        890     753     191     138      70     155     122

  Fragments arranged by size: 

                 890     753     191     155     138     122      70
 
BsmI GAATG_Cn'

Cuts at:      0     375    2319 
   Size:        375    1944
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     624    1418    2090    2319 
   Size:        624     794     672     229

  Fragments arranged by size: 

                 794     672     624     229
 
Bsp1286I G_dGCh'C

Cuts at:      0     116     129     841     890     950    1277    1339    1643 
   Size:        116      13     712      49      60     327      62     304

Cuts at:   1643    1657    1834    1913    1972    2042    2197    2319 
   Size:         14     177      79      59      70     155     122

  Fragments arranged by size: 

                 712     327     304     177     155     122     116      79
                  70      62      60      59      49      14      13
 
BspEI T'CCGG_A

Cuts at:      0     545    1064    2319 
   Size:        545     519    1255

  Fragments arranged by size: 

                1255     545     519
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     449    2319 
   Size:        449    1870
 
BsrBI CCG'CTC

Cuts at:      0     756    2319 
   Size:        756    1563
 
BsrDI GCAATG_nn'

Cuts at:      0     475    2319 
   Size:        475    1844
 
BsrFI r'CCGG_y

Cuts at:      0    1239    1558    1871    2319 
   Size:       1239     319     313     448

  Fragments arranged by size: 

                1239     448     319     313
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     746    2319 
   Size:        746    1573
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     521     981    2319 
   Size:        521     460    1338

  Fragments arranged by size: 

                1338     521     460
 
BstYI r'GATC_y

Cuts at:      0     221     542     720    1202    1754    2259    2319 
   Size:        221     321     178     482     552     505      60

  Fragments arranged by size: 

                 552     505     482     321     221     178      60
 
Bsu36I CC'TnA_GG

Cuts at:      0     241    2319 
   Size:        241    2078
 
BtgI C'CryG_G

Cuts at:      0     202     767     794    1745    2319 
   Size:        202     565      27     951     574

  Fragments arranged by size: 

                 951     574     565     202      27
 
BtsI GCAGTG_nn'

Cuts at:      0     744     948    1229    1924    2319 
   Size:        744     204     281     695     395

  Fragments arranged by size: 

                 744     695     395     281     204
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2073    2108    2319 
   Size:       2073      35     211

  Fragments arranged by size: 

                2073     211      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     181    2319 
   Size:        181    2138
 
EaeI y'GGCC_r

Cuts at:      0     272     983    1250    1418    2319 
   Size:        272     711     267     168     901

  Fragments arranged by size: 

                 901     711     272     267     168
 
EarI CTCTTCn'nnn_

Cuts at:      0     134    1013    2197    2319 
   Size:        134     879    1184     122

  Fragments arranged by size: 

                1184     879     134     122
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     282    2319 
   Size:        282    2037
 
EcoICRI GAG'CTC

Cuts at:      0     888    1970    2195    2319 
   Size:        888    1082     225     124

  Fragments arranged by size: 

                1082     888     225     124
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1075    1179    1425    1507    1846    1906    2011    2122 
   Size:       1075     104     246      82     339      60     105     111

Cuts at:   2122    2284    2319 
   Size:        162      35

  Fragments arranged by size: 

                1075     339     246     162     111     105     104      82
                  60      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     603     966    1336    1708    2319 
   Size:        603     363     370     372     611

  Fragments arranged by size: 

                 611     603     372     370     363
 
EcoRI G'AATT_C

Cuts at:      0     730    2319 
   Size:        730    1589
 
HaeII r_GCGC'y

Cuts at:      0     110     249    1246    2319 
   Size:        110     139     997    1073

  Fragments arranged by size: 

                1073     997     139     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1126    1158    2319 
   Size:       1126      32    1161

  Fragments arranged by size: 

                1161    1126      32
 
HindIII A'AGCT_T

Cuts at:      0    2272    2319 
   Size:       2272      47
 
KasI G'GCGC_C

Cuts at:      0     106    2319 
   Size:        106    2213
 
MfeI C'AATT_G

Cuts at:      0     338    2319 
   Size:        338    1981
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2099    2319 
   Size:       2099     220
 
MscI TGG'CCA

Cuts at:      0    1420    2319 
   Size:       1420     899
 
MslI CAynn'nnrTG

Cuts at:      0     100     676     781     793    1648    1837    2319 
   Size:        100     576     105      12     855     189     482

  Fragments arranged by size: 

                 855     576     482     189     105     100      12
 
MspA1I CmG'CkG

Cuts at:      0      63     204     354     521    1863    1878    2070    2245 
   Size:         63     141     150     167    1342      15     192     175

Cuts at:   2245    2319 
   Size:         74

  Fragments arranged by size: 

                1342     192     175     167     150     141      74      63
                  15
 
NaeI GCC'GGC

Cuts at:      0    1241    1560    2319 
   Size:       1241     319     759

  Fragments arranged by size: 

                1241     759     319
 
NarI GG'CG_CC

Cuts at:      0     107    2319 
   Size:        107    2212
 
NcoI C'CATG_G

Cuts at:      0     767    1745    2319 
   Size:        767     978     574

  Fragments arranged by size: 

                 978     767     574
 
NgoMIV G'CCGG_C

Cuts at:      0    1239    1558    2319 
   Size:       1239     319     761

  Fragments arranged by size: 

                1239     761     319
 
NspI r_CATG'y

Cuts at:      0     933    1719    1854    2319 
   Size:        933     786     135     465

  Fragments arranged by size: 

                 933     786     465     135
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1746    2319 
   Size:       1746     573
 
PmlI CAC'GTG

Cuts at:      0    1209    2319 
   Size:       1209    1110
 
PpuMI rG'GwC_Cy

Cuts at:      0     966    1708    2319 
   Size:        966     742     611

  Fragments arranged by size: 

                 966     742     611
 
PshAI GACnn'nnGTC

Cuts at:      0    2164    2319 
   Size:       2164     155
 
PspOMI G'GGCC_C

Cuts at:      0    1335    1909    2319 
   Size:       1335     574     410

  Fragments arranged by size: 

                1335     574     410
 
PstI C_TGCA'G

Cuts at:      0     167     425     584     751    1315    1811    2215    2319 
   Size:        167     258     159     167     564     496     404     104

  Fragments arranged by size: 

                 564     496     404     258     167     167     159     104
            
 
PvuII CAG'CTG

Cuts at:      0     354    1863    2070    2245    2319 
   Size:        354    1509     207     175      74

  Fragments arranged by size: 

                1509     354     207     175      74
 
SacI G_AGCT'C

Cuts at:      0     890    1972    2197    2319 
   Size:        890    1082     225     122

  Fragments arranged by size: 

                1082     890     225     122
 
SacII CC_GC'GG

Cuts at:      0     205    2319 
   Size:        205    2114
 
SanDI GG'GwC_CC

Cuts at:      0    1708    2319 
   Size:       1708     611
 
SapI GCTCTTCn'nnn_

Cuts at:      0     134    2319 
   Size:        134    2185
 
SbfI CC_TGCA'GG

Cuts at:      0     584    2319 
   Size:        584    1735
 
ScaI AGT'ACT

Cuts at:      0     382    2319 
   Size:        382    1937
 
SexAI A'CCwGG_T

Cuts at:      0     977    1136    2319 
   Size:        977     159    1183

  Fragments arranged by size: 

                1183     977     159
 
SfcI C'TryA_G

Cuts at:      0     163     330     421     580     747    1311    1584    1807 
   Size:        163     167      91     159     167     564     273     223

Cuts at:   1807    2211    2319 
   Size:        404     108

  Fragments arranged by size: 

                 564     404     273     223     167     167     163     159
                 108      91
 
SfoI GGC'GCC

Cuts at:      0     108    2319 
   Size:        108    2211
 
SmaI CCC'GGG

Cuts at:      0     964    2319 
   Size:        964    1355
 
SmlI C'TyrA_G

Cuts at:      0     811    1174    1378    2062    2319 
   Size:        811     363     204     684     257

  Fragments arranged by size: 

                 811     684     363     257     204
 
SphI G_CATG'C

Cuts at:      0     933    1719    2319 
   Size:        933     786     600

  Fragments arranged by size: 

                 933     786     600
 
StyI C'CwwG_G

Cuts at:      0     767    1478    1745    2319 
   Size:        767     711     267     574

  Fragments arranged by size: 

                 767     711     574     267
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     670     725    1816    2319 
   Size:        670      55    1091     503

  Fragments arranged by size: 

                1091     670     503      55
 
TatI w'GTAC_w

Cuts at:      0     380     866    2319 
   Size:        380     486    1453

  Fragments arranged by size: 

                1453     486     380
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1744    1776    2319 
   Size:       1744      32     543

  Fragments arranged by size: 

                1744     543      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      63    1134    2319 
   Size:         63    1071    1185

  Fragments arranged by size: 

                1185    1071      63
 
XmaI C'CCGG_G

Cuts at:      0     962    2319 
   Size:        962    1357
 
XmnI GAAnn'nnTTC

Cuts at:      0     347     916    2319 
   Size:        347     569    1403

  Fragments arranged by size: 

                1403     569     347
 
ZraI GAC'GTC

Cuts at:      0    2298    2319 
   Size:       2298      21
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AhdI     AleI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrFI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     BtsI    CspCI     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    HaeII    Hin4I  HindIII     KasI     MfeI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
    PflMI     PmlI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SmaI     SmlI     SphI     StyI    TaqII     TatI     TstI     XcmI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AlfI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BmtI    BsaBI    BsiWI    BspHI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI     DraI
   DraIII     EagI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
   HincII     HpaI     KpnI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PpiI     PsiI
    PspXI     PsrI     PvuI    RsrII     SalI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XhoI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com