Restriction map for pF1KA1646
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

Acc65I      AclI        AfaI        AflII       AgeI        
AhdI        Alw44I      AocI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BamHI       BbrPI       BbvCI       
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaI        
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspHI       BsrBI       BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
KspI        MaeI        MfeI        MluI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII         378  GACGTC               GACGT^C
AccI          649  GTCGAC               GT^[AC][GT]AC
AcsI         1369  AAATTC               [GA]^AATT[CT]
AflIII       1184  ACATGT               A^C[GA][CT]GT
AlwI         1015  GGATC                GGATC(4/5)
ApaI          115  GGGCCC               GGGCC^C
ApoI         1369  AAATTC               [GA]^AATT[CT]
AspHI         272  GAGCAC               G[AT]GC[AT]^C
AspI          981  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI          127  GGCGCC               GGCGC^C
BbsI         1248  GTCTTC               GAAGAC(2/6)
BcgI          760  GCAGAAACCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         1208  GCCCCAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1248  GTCTTC               GAAGAC(2/6)
BsaBI         696  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       272  GAGCAC               G[AT]GC[AT]^C
BspLU11I     1184  ACATGT               A^CATGT
BspMI         300  GCAGGT               ACCTGC(4/8)
BsrFI         124  GCCGGC               [GA]^CCGG[CT]
BssSI        1571  CACGAG               CACGAG(-5/-1)
BstYI        1015  GGATCT               [GA]^GATC[CT]
Cfr10I        124  GCCGGC               [GA]^CCGG[CT]
DraI          945  TTTAAA               TTT^AAA
DraIII       1006  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         1582  GAAGAG               CTCTTC(1/4)
EcoNI        1413  CCTCAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI          127  GGCGCC               G^GCGCC
Ksp632I      1582  GAAGAG               CTCTTC(1/4)
MamI          696  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1015  GGATCT               [GA]^GATC[CT]
NaeI          124  GCCGGC               GCC^GGC
NarI          127  GGCGCC               GG^CGCC
NcoI          803  CCATGG               C^CATGG
NgoMIV        124  GCCGGC               G^CCGGC
PflFI         981  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1511  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          346  CTGCAG               CTGCA^G
SalI          649  GTCGAC               G^TCGAC
SfcI          346  CTGCAG               C^T[GA][CT]AG
SfoI          127  GGCGCC               GGC^GCC
SmlI         1414  CTCAAG               C^T[CT][GA]AG
Tth111I       981  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII        1015  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccIII        692  TCCGGA               T^CCGGA
             1268  TCCGGA              
AcyI          127  GGCGCC               G[GA]^CG[CT]C
              378  GACGTC              
Aor51HI        56  AGCGCT               AGC^GCT
             1433  AGCGCT              
AvaI          111  CCCGGG               C^[CT]CG[GA]G
              665  CCCGGG              
BalI          800  TGGCCA               TGG^CCA
             1166  TGGCCA              
BsaHI         127  GGCGCC               G[GA]^CG[CT]C
              378  GACGTC              
BsaWI         692  TCCGGA               [AT]^CCGG[AT]
             1268  TCCGGA              
BseAI         692  TCCGGA               T^CCGGA
             1268  TCCGGA              
BsmBI        1479  CGTCTC               CGTCTC(1/5)
             1241  GAGACG              
BsoBI         111  CCCGGG               C^[CT]CG[GA]G
              665  CCCGGG              
BspEI         692  TCCGGA               T^CCGGA
             1268  TCCGGA              
BstAPI         30  GCAATCCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              348  GCAGACCCTGC         
DpnII         168  GATC                 ^GATC
             1016  GATC                
DrdI          565  GACGGCATCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1504  GACGGGGAGGTC        
DsaI          743  CCGTGG               C^C[GA][CT]GG
              803  CCATGG              
EaeI          800  TGGCCA               [CT]^GGCC[GA]
             1166  TGGCCA              
Eco47III       56  AGCGCT               AGC^GCT
             1433  AGCGCT              
Hin1I         127  GGCGCC               G[GA]^CG[CT]C
              378  GACGTC              
HincII        649  GTCGAC               GT[CT]^[GA]AC
              717  GTCAAC              
HindII        649  GTCGAC               GT[CT]^[GA]AC
              717  GTCAAC              
MboI          168  GATC                 ^GATC
             1016  GATC                
MluNI         800  TGGCCA               TGG^CCA
             1166  TGGCCA              
MroI          692  TCCGGA               T^CCGGA
             1268  TCCGGA              
MscI          800  TGGCCA               TGG^CCA
             1166  TGGCCA              
MseI          548  TTAA                 T^TAA
              946  TTAA                
NdeII         168  GATC                 ^GATC
             1016  GATC                
NspI          513  ACATGC               [GA]CATG^[CT]
             1184  ACATGT              
Sau3AI        168  GATC                 ^GATC
             1016  GATC                
SmaI          111  CCCGGG               CCC^GGG
              665  CCCGGG              
TfiI          701  GAATC                G^A[AT]TC
             1587  GAATC               
Tru9I         548  TTAA                 T^TAA
              946  TTAA                
Tsp45I        304  GTGAC                ^GT[GC]AC
              335  GTCAC               
XmaCI         111  CCCGGG               C^CCGGG
              665  CCCGGG              
XmaI          111  CCCGGG               C^CCGGG
              665  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AvaII       
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NlaIII      NlaIV       PleI        PvuII       
Sau96I      ScrFI       SfaNI       StyI        TaqI        
TseI        Tsp509I     TspRI       TthHB8I     
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