Restriction map for pF1KA1646
 (Linear) (Six-base) MAPSORT of: KIAA1646.seq  Check: 6016  from: 1  to: 1611



KIAA1646.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     291    1611 
   Size:        291    1320
 
AatII G_ACGT'C

Cuts at:      0     382    1611 
   Size:        382    1229
 
AccI GT'mk_AC

Cuts at:      0     650    1611 
   Size:        650     961
 
AfeI AGC'GCT

Cuts at:      0      58    1435    1611 
   Size:         58    1377     176

  Fragments arranged by size: 

                1377     176      58
 
AflIII A'CryG_T

Cuts at:      0    1184    1611 
   Size:       1184     427
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1541    1611 
   Size:       1541      70
 
AlwNI CAG_nnn'CTG

Cuts at:      0     354    1080    1493    1611 
   Size:        354     726     413     118

  Fragments arranged by size: 

                 726     413     354     118
 
ApaI G_GGCC'C

Cuts at:      0     119    1611 
   Size:        119    1492
 
ApoI r'AATT_y

Cuts at:      0    1369    1611 
   Size:       1369     242
 
AvaI C'yCGr_G

Cuts at:      0     111     665    1611 
   Size:        111     554     946

  Fragments arranged by size: 

                 946     554     111
 
BanI G'GyrC_C

Cuts at:      0     127     281     462     748    1611 
   Size:        127     154     181     286     863

  Fragments arranged by size: 

                 863     286     181     154     127
 
BanII G_rGCy'C

Cuts at:      0      80      90     112     119    1210    1611 
   Size:         80      10      22       7    1091     401

  Fragments arranged by size: 

                1091     401      80      22      10       7
 
BbeI G_GCGC'C

Cuts at:      0     131    1611 
   Size:        131    1480
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1241    1611 
   Size:       1241     370
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     749     783    1611 
   Size:        749      34     828

  Fragments arranged by size: 

                 828     749      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1214    1611 
   Size:       1214     397
 
Bme1580I G_kGCm'C

Cuts at:      0     119     751    1445    1611 
   Size:        119     632     694     166

  Fragments arranged by size: 

                 694     632     166     119
 
BmgBI CAC'GTC

Cuts at:      0    1131    1611 
   Size:       1131     480
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     677    1611 
   Size:        677     934
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     388    1102    1263    1611 
   Size:         94     294     714     161     348

  Fragments arranged by size: 

                 714     348     294     161      94
 
Bpu10I CC'TnA_GC

Cuts at:      0     477    1611 
   Size:        477    1134
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1399    1611 
   Size:       1399     212
 
BsaBI GATnn'nnATC

Cuts at:      0     700    1611 
   Size:        700     911
 
BsaHI Gr'CG_yC

Cuts at:      0     128     379    1611 
   Size:        128     251    1232

  Fragments arranged by size: 

                1232     251     128
 
BsaWI w'CCGG_w

Cuts at:      0     692    1268    1611 
   Size:        692     576     343

  Fragments arranged by size: 

                 692     576     343
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     316     346     523     553    1611 
   Size:        316      30     177      30    1058

  Fragments arranged by size: 

                1058     316     177      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     337     651    1099    1102    1150    1611 
   Size:        337     314     448       3      48     461

  Fragments arranged by size: 

                 461     448     337     314      48       3
 
BseYI C'CCAG_C

Cuts at:      0     861    1611 
   Size:        861     750
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595     983    1458    1501    1611 
   Size:        595     388     475      43     110

  Fragments arranged by size: 

                 595     475     388     110      43
 
BsiHKAI G_wGCw'C

Cuts at:      0     276    1611 
   Size:        276    1335
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1235    1485    1611 
   Size:       1235     250     126

  Fragments arranged by size: 

                1235     250     126
 
Bsp1286I G_dGCh'C

Cuts at:      0      80      90     112     119     276     751    1210    1445 
   Size:         80      10      22       7     157     475     459     235

Cuts at:   1445    1611 
   Size:        166

  Fragments arranged by size: 

                 475     459     235     166     157      80      22      10
                   7
 
BspEI T'CCGG_A

Cuts at:      0     692    1268    1611 
   Size:        692     576     343

  Fragments arranged by size: 

                 692     576     343
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     291    1611 
   Size:        291    1320
 
BsrFI r'CCGG_y

Cuts at:      0     124    1611 
   Size:        124    1487
 
BssSI C'ACGA_G

Cuts at:      0    1571    1611 
   Size:       1571      40
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      36     354    1611 
   Size:         36     318    1257

  Fragments arranged by size: 

                1257     318      36
 
BstYI r'GATC_y

Cuts at:      0    1015    1611 
   Size:       1015     596
 
BtgI C'CryG_G

Cuts at:      0     743     803    1611 
   Size:        743      60     808

  Fragments arranged by size: 

                 808     743      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     156    1611 
   Size:        156    1455
 
BtsI GCAGTG_nn'

Cuts at:      0     219     966    1545    1611 
   Size:        219     747     579      66

  Fragments arranged by size: 

                 747     579     219      66
 
DraI TTT'AAA

Cuts at:      0     947    1611 
   Size:        947     664
 
DraIII CAC_nnn'GTG

Cuts at:      0    1011    1611 
   Size:       1011     600
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     571    1510    1611 
   Size:        571     939     101

  Fragments arranged by size: 

                 939     571     101
 
EaeI y'GGCC_r

Cuts at:      0     800    1166    1611 
   Size:        800     366     445

  Fragments arranged by size: 

                 800     445     366
 
EarI CTCTTCn'nnn_

Cuts at:      0    1577    1611 
   Size:       1577      34
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      34     120     599    1611 
   Size:         34      86     479    1012

  Fragments arranged by size: 

                1012     479      86      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     388    1102    1263    1611 
   Size:         94     294     714     161     348

  Fragments arranged by size: 

                 714     348     294     161      94
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1417    1611 
   Size:       1417     194
 
EcoO109I rG'GnC_Cy

Cuts at:      0     115    1215    1512    1611 
   Size:        115    1100     297      99

  Fragments arranged by size: 

                1100     297     115      99
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1452    1611 
   Size:       1452     159
 
HaeII r_GCGC'y

Cuts at:      0      60     131     644     775    1437    1611 
   Size:         60      71     513     131     662     174

  Fragments arranged by size: 

                 662     513     174     131      71      60
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     133     165     799     831    1289    1321    1611 
   Size:        133      32     634      32     458      32     290

  Fragments arranged by size: 

                 634     458     290     133      32      32      32
 
HincII GTy'rAC

Cuts at:      0     651     719    1611 
   Size:        651      68     892

  Fragments arranged by size: 

                 892     651      68
 
KasI G'GCGC_C

Cuts at:      0     127    1611 
   Size:        127    1484
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1508    1611 
   Size:       1508     103
 
MscI TGG'CCA

Cuts at:      0     802    1168    1611 
   Size:        802     366     443

  Fragments arranged by size: 

                 802     443     366
 
MslI CAynn'nnrTG

Cuts at:      0     808     971    1183    1611 
   Size:        808     163     212     428

  Fragments arranged by size: 

                 808     428     212     163
 
MspA1I CmG'CkG

Cuts at:      0      27     287     330    1080    1554    1611 
   Size:         27     260      43     750     474      57

  Fragments arranged by size: 

                 750     474     260      57      43      27
 
NaeI GCC'GGC

Cuts at:      0     126    1611 
   Size:        126    1485
 
NarI GG'CG_CC

Cuts at:      0     128    1611 
   Size:        128    1483
 
NcoI C'CATG_G

Cuts at:      0     803    1611 
   Size:        803     808
 
NgoMIV G'CCGG_C

Cuts at:      0     124    1611 
   Size:        124    1487
 
NspI r_CATG'y

Cuts at:      0     517    1188    1611 
   Size:        517     671     423

  Fragments arranged by size: 

                 671     517     423
 
PasI CC'CwG_GG

Cuts at:      0    1211    1611 
   Size:       1211     400
 
PciI A'CATG_T

Cuts at:      0    1184    1611 
   Size:       1184     427
 
PfoI T'CCnGG_A

Cuts at:      0    1492    1611 
   Size:       1492     119
 
PpuMI rG'GwC_Cy

Cuts at:      0    1512    1611 
   Size:       1512      99
 
PspOMI G'GGCC_C

Cuts at:      0     115    1611 
   Size:        115    1496
 
PstI C_TGCA'G

Cuts at:      0     350    1611 
   Size:        350    1261
 
PvuII CAG'CTG

Cuts at:      0     330    1080    1554    1611 
   Size:        330     750     474      57

  Fragments arranged by size: 

                 750     474     330      57
 
SalI G'TCGA_C

Cuts at:      0     649    1611 
   Size:        649     962
 
SfcI C'TryA_G

Cuts at:      0     346    1611 
   Size:        346    1265
 
SfoI GGC'GCC

Cuts at:      0     129    1611 
   Size:        129    1482
 
SmaI CCC'GGG

Cuts at:      0     113     667    1611 
   Size:        113     554     944

  Fragments arranged by size: 

                 944     554     113
 
SmlI C'TyrA_G

Cuts at:      0    1414    1611 
   Size:       1414     197
 
StyI C'CwwG_G

Cuts at:      0     527     803    1021    1611 
   Size:        527     276     218     590

  Fragments arranged by size: 

                 590     527     276     218
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      33    1611 
   Size:         33    1578
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     316     348     715     747    1611 
   Size:        316      32     367      32     864

  Fragments arranged by size: 

                 864     367     316      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     984    1611 
   Size:        984     627
 
XmaI C'CCGG_G

Cuts at:      0     111     665    1611 
   Size:        111     554     946

  Fragments arranged by size: 

                 946     554     111
 
ZraI GAC'GTC

Cuts at:      0     380    1611 
   Size:        380    1231
 

 Enzymes that do cut:

     AarI    AatII     AccI     AfeI   AflIII     AlfI    AlwNI     ApaI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI     BcgI     BglI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspMI    BsrFI    BssSI   BstAPI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EarI     EciI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI     PfoI    PpuMI   PspOMI     PstI    PvuII     SalI
     SfcI     SfoI     SmaI     SmlI     StyI    TaqII     TstI  Tth111I
     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AcuI    AflII     AgeI     AhdI     AleI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
    BciVI     BclI    BfrBI    BglII     BlpI     BmtI     BplI     BsaI
    BsaAI    BsiEI    BsiWI     BsmI    BspHI    BsrBI    BsrDI    BsrGI
   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
     EagI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     XbaI     XcmI
     XhoI     XmnI
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