Restriction map for pF1KA1619
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvrII       BalI        
BcgI        BclI        BfrI        BglII       BlnI        
BsaBI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRV       FbaI        FseI        
HgaI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MscI        MunI        NcoI        NdeI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII        2061  CGCG                 CG^CG
AccIII        563  TCCGGA               T^CCGGA
AcsI          559  GAATTC               [GA]^AATT[CT]
AcyI         2135  GGCGCC               G[GA]^CG[CT]C
AflIII       1240  ACACGT               A^C[GA][CT]GT
AlwNI          41  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         2440  GGGCCC               GGGCC^C
ApoI          559  GAATTC               [GA]^AATT[CT]
BamHI        1361  GGATCC               G^GATCC
BbeI         2135  GGCGCC               GGCGC^C
BbrPI        1241  CACGTG               CAC^GTG
BciVI        1698  GGATAC               GTATCC(6/5)
BsaAI        1241  CACGTG               [CT]AC^GT[GA]
BsaHI        2135  GGCGCC               G[GA]^CG[CT]C
BsaWI         563  TCCGGA               [AT]^CCGG[AT]
BseAI         563  TCCGGA               T^CCGGA
BsmBI         871  CGTCTC               CGTCTC(1/5)
BsmI          569  GCATTC               GAATGC(1/-1)
BspEI         563  TCCGGA               T^CCGGA
BspMI        1331  ACCTGC               ACCTGC(4/8)
BsrBI        1728  CCGCTC               CCGCTC(-3/-3)
BstUI        2061  CGCG                 CG^CG
DsaI          504  CCGTGG               C^C[GA][CT]GG
EaeI         1966  CGGCCA               [CT]^GGCC[GA]
EcoNI         191  CCTCAGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         559  GAATTC               G^AATTC
EcoT14I      1152  CCAAGG               C^C[AT][AT]GG
Hin1I        2135  GGCGCC               G[GA]^CG[CT]C
KasI         2135  GGCGCC               G^GCGCC
MaeII        1242  ACGT                 A^CGT
MroI          563  TCCGGA               T^CCGGA
MseI          347  TTAA                 T^TAA
MvnI         2061  CGCG                 CG^CG
NaeI         2372  GCCGGC               GCC^GGC
NarI         2135  GGCGCC               GG^CGCC
NgoMIV       2372  GCCGGC               G^CCGGC
NheI         2049  GCTAGC               G^CTAGC
NspI          394  GCATGT               [GA]CATG^[CT]
PmaCI        1241  CACGTG               CAC^GTG
PmlI         1241  CACGTG               CAC^GTG
PshAI        1296  GACACCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI          121  GAAGAGC              GCTCTTC(1/4)
SfoI         2135  GGCGCC               GGC^GCC
SmaI         1787  CCCGGG               CCC^GGG
StyI         1152  CCAAGG               C^C[AT][AT]GG
TfiI         1138  GATTC                G^A[AT]TC
Tru9I         347  TTAA                 T^TAA
XbaI           79  TCTAGA               T^CTAGA
XmaCI        1787  CCCGGG               C^CCGGG
XmaI         1787  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AviII        1145  TGCGCA               TGC^GCA
             1600  TGCGCA              
BbsI          984  GAAGAC               GAAGAC(2/6)
             1722  GAAGAC              
BlpI          969  GCTGAGC              GC^T[ACGT]AGC
             2338  GCTGAGC             
Bpu1102I      969  GCTGAGC              GC^T[ACGT]AGC
             2338  GCTGAGC             
BpuAI         984  GAAGAC               GAAGAC(2/6)
             1722  GAAGAC              
BsgI          425  GTGCAG               GTGCAG(16/14)
              689  GTGCAG              
BsrDI        1142  CATTGC               GCAATG(2/0)
             2055  CATTGC              
BsrFI        2334  ACCGGC               [GA]^CCGG[CT]
             2372  GCCGGC              
CelII         969  GCTGAGC              GC^T[ACGT]AGC
             2338  GCTGAGC             
Cfr10I       2334  ACCGGC               [GA]^CCGG[CT]
             2372  GCCGGC              
EarI          121  GAAGAG               CTCTTC(1/4)
             1405  GAAGAG              
EcoT22I       392  ATGCAT               ATGCA^T
              628  ATGCAT              
FspI         1145  TGCGCA               TGC^GCA
             1600  TGCGCA              
HaeII        1079  GGCGCT               [GA]GCGC^[CT]
             2135  GGCGCC              
Ksp632I       121  GAAGAG               CTCTTC(1/4)
             1405  GAAGAG              
MslI          385  CACCTCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              949  CACTTCTATG          
NsiI          392  ATGCAT               ATGCA^T
              628  ATGCAT              
PflMI         499  CCAGCCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1431  CCAGTCTGTGG         
SacI          124  GAGCTC               GAGCT^C
             2520  GAGCTC              
SmlI          936  CTCAAG               C^T[CT][GA]AG
             1261  CTCAAG              
TaqI          467  TCGA                 T^CGA
             1677  TCGA                
TthHB8I       467  TCGA                 T^CGA
             1677  TCGA                
Van91I        499  CCAGCCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1431  CCAGTCTGTGG         
XcmI         2277  CCAGATCATCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2348  CCAATGGCTTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaI        AvaII       BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BssSI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StuI        TseI        Tsp45I      
Tsp509I     TspRI       XhoII       
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