Restriction map for
pF1KA1567
(Linear) (Six-base) MAPSORT of: KIAA1567.seq Check: 3402 from: 1 to: 2583
KIAA1567.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AccI GT'mk_AC
Cuts at: 0 1110 2583
Size: 1110 1473
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1690 2583
Size: 373 1317 893
Fragments arranged by size:
1317 893 373
AflIII A'CryG_T
Cuts at: 0 266 1575 2583
Size: 266 1309 1008
Fragments arranged by size:
1309 1008 266
AleI CACnn'nnGTG
Cuts at: 0 496 2209 2374 2583
Size: 496 1713 165 209
Fragments arranged by size:
1713 496 209 165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1319 2583
Size: 1319 1264
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1471 1503 2271 2303 2583
Size: 1471 32 768 32 280
Fragments arranged by size:
1471 768 280 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1929 2349 2410 2583
Size: 1929 420 61 173
Fragments arranged by size:
1929 420 173 61
ApaLI G'TGCA_C
Cuts at: 0 2382 2583
Size: 2382 201
ApoI r'AATT_y
Cuts at: 0 739 1162 2270 2583
Size: 739 423 1108 313
Fragments arranged by size:
1108 739 423 313
AvaI C'yCGr_G
Cuts at: 0 991 2583
Size: 991 1592
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 188 221 2583
Size: 188 33 2362
Fragments arranged by size:
2362 188 33
BamHI G'GATC_C
Cuts at: 0 2517 2583
Size: 2517 66
BanII G_rGCy'C
Cuts at: 0 651 1199 1901 2583
Size: 651 548 702 682
Fragments arranged by size:
702 682 651 548
BbsI GAAGACnn'nnnn_
Cuts at: 0 1642 2583
Size: 1642 941
BclI T'GATC_A
Cuts at: 0 1061 2583
Size: 1061 1522
BglII A'GATC_T
Cuts at: 0 1078 1886 2583
Size: 1078 808 697
Fragments arranged by size:
1078 808 697
BlpI GC'TnA_GC
Cuts at: 0 650 2583
Size: 650 1933
Bme1580I G_kGCm'C
Cuts at: 0 419 2386 2583
Size: 419 1967 197
Fragments arranged by size:
1967 419 197
BmrI ACTGGGnnnn_n'
Cuts at: 0 490 2583
Size: 490 2093
BmtI G_CTAG'C
Cuts at: 0 32 2583
Size: 32 2551
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2402 2583
Size: 2402 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2326 2583
Size: 2326 257
Bpu10I CC'TnA_GC
Cuts at: 0 2534 2583
Size: 2534 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 582 766 2583
Size: 582 184 1817
Fragments arranged by size:
1817 582 184
BsaBI GATnn'nnATC
Cuts at: 0 131 2465 2583
Size: 131 2334 118
Fragments arranged by size:
2334 131 118
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 463 493 550 580 1471 1501 1751 1781
Size: 463 30 57 30 891 30 250 30
Cuts at: 1781 2583
Size: 802
Fragments arranged by size:
891 802 463 250 57 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1891 2583
Size: 1891 692
BseYI C'CCAG_C
Cuts at: 0 9 412 728 1779 1840 2561 2583
Size: 9 403 316 1051 61 721 22
Fragments arranged by size:
1051 721 403 316 61 22 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1487 1888 2177 2583
Size: 1487 401 289 406
Fragments arranged by size:
1487 406 401 289
BsiEI CG_ry'CG
Cuts at: 0 2514 2583
Size: 2514 69
BsiHKAI G_wGCw'C
Cuts at: 0 1199 1901 2386 2583
Size: 1199 702 485 197
Fragments arranged by size:
1199 702 485 197
BsmI GAATG_Cn'
Cuts at: 0 1427 2583
Size: 1427 1156
Bsp1286I G_dGCh'C
Cuts at: 0 419 651 1199 1901 2386 2583
Size: 419 232 548 702 485 197
Fragments arranged by size:
702 548 485 419 232 197
BspHI T'CATG_A
Cuts at: 0 2469 2583
Size: 2469 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 600 2583
Size: 600 1983
BsrBI CCG'CTC
Cuts at: 0 1762 2583
Size: 1762 821
BsrDI GCAATG_nn'
Cuts at: 0 1805 2196 2583
Size: 1805 391 387
Fragments arranged by size:
1805 391 387
BsrFI r'CCGG_y
Cuts at: 0 1844 2099 2583
Size: 1844 255 484
Fragments arranged by size:
1844 484 255
BsrGI T'GTAC_A
Cuts at: 0 269 886 2583
Size: 269 617 1697
Fragments arranged by size:
1697 617 269
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1081 1465 2419 2583
Size: 1081 384 954 164
Fragments arranged by size:
1081 954 384 164
BstBI TT'CG_AA
Cuts at: 0 2067 2583
Size: 2067 516
BstEII G'GTnAC_C
Cuts at: 0 774 2583
Size: 774 1809
BstYI r'GATC_y
Cuts at: 0 1078 1886 2517 2583
Size: 1078 808 631 66
Fragments arranged by size:
1078 808 631 66
Bsu36I CC'TnA_GG
Cuts at: 0 2522 2583
Size: 2522 61
BtgI C'CryG_G
Cuts at: 0 2007 2583
Size: 2007 576
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1035 2583
Size: 1035 1548
BtsI GCAGTG_nn'
Cuts at: 0 2327 2583
Size: 2327 256
DraI TTT'AAA
Cuts at: 0 354 2583
Size: 354 2229
DraIII CAC_nnn'GTG
Cuts at: 0 1465 1610 1612 2583
Size: 1465 145 2 971
Fragments arranged by size:
1465 971 145 2
EaeI y'GGCC_r
Cuts at: 0 829 1601 1833 2047 2101 2511 2583
Size: 829 772 232 214 54 410 72
Fragments arranged by size:
829 772 410 232 214 72 54
EagI C'GGCC_G
Cuts at: 0 2511 2583
Size: 2511 72
EarI CTCTTCn'nnn_
Cuts at: 0 329 1094 1114 2155 2583
Size: 329 765 20 1041 428
Fragments arranged by size:
1041 765 428 329 20
EcoICRI GAG'CTC
Cuts at: 0 1197 1899 2583
Size: 1197 702 684
Fragments arranged by size:
1197 702 684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1690 2326 2583
Size: 373 1317 636 257
Fragments arranged by size:
1317 636 373 257
EcoNI CCTnn'n_nnAGG
Cuts at: 0 483 545 2376 2583
Size: 483 62 1831 207
Fragments arranged by size:
1831 483 207 62
EcoO109I rG'GnC_Cy
Cuts at: 0 13 1724 1926 2583
Size: 13 1711 202 657
Fragments arranged by size:
1711 657 202 13
EcoRI G'AATT_C
Cuts at: 0 2270 2583
Size: 2270 313
HaeII r_GCGC'y
Cuts at: 0 10 2583
Size: 10 2573
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 699 731 1629 1661 1751 1783 2583
Size: 699 32 898 32 90 32 800
Fragments arranged by size:
898 800 699 90 32 32 32
HincII GTy'rAC
Cuts at: 0 180 2583
Size: 180 2403
HindIII A'AGCT_T
Cuts at: 0 404 2583
Size: 404 2179
MfeI C'AATT_G
Cuts at: 0 290 2583
Size: 290 2293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 1560 2583
Size: 51 1509 1023
Fragments arranged by size:
1509 1023 51
MscI TGG'CCA
Cuts at: 0 1603 1835 2049 2583
Size: 1603 232 214 534
Fragments arranged by size:
1603 534 232 214
MslI CAynn'nnrTG
Cuts at: 0 140 422 496 2209 2372 2374 2474 2583
Size: 140 282 74 1713 163 2 100 109
Fragments arranged by size:
1713 282 163 140 109 100 74 2
MspA1I CmG'CkG
Cuts at: 0 1395 2361 2583
Size: 1395 966 222
Fragments arranged by size:
1395 966 222
NheI G'CTAG_C
Cuts at: 0 28 2583
Size: 28 2555
NspI r_CATG'y
Cuts at: 0 270 1287 2078 2583
Size: 270 1017 791 505
Fragments arranged by size:
1017 791 505 270
PciI A'CATG_T
Cuts at: 0 266 2583
Size: 266 2317
PflMI CCAn_nnn'nTGG
Cuts at: 0 543 2583
Size: 543 2040
PfoI T'CCnGG_A
Cuts at: 0 1922 2583
Size: 1922 661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1471 1503 2583
Size: 1471 32 1080
Fragments arranged by size:
1471 1080 32
PpuMI rG'GwC_Cy
Cuts at: 0 13 1926 2583
Size: 13 1913 657
Fragments arranged by size:
1913 657 13
PspXI vC'TCGA_Gb
Cuts at: 0 991 2583
Size: 991 1592
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2330 2362 2583
Size: 2330 32 221
Fragments arranged by size:
2330 221 32
PstI C_TGCA'G
Cuts at: 0 2334 2583
Size: 2334 249
PvuII CAG'CTG
Cuts at: 0 1395 2361 2583
Size: 1395 966 222
Fragments arranged by size:
1395 966 222
SacI G_AGCT'C
Cuts at: 0 1199 1901 2583
Size: 1199 702 682
Fragments arranged by size:
1199 702 682
SanDI GG'GwC_CC
Cuts at: 0 13 2583
Size: 13 2570
SapI GCTCTTCn'nnn_
Cuts at: 0 1114 2583
Size: 1114 1469
SexAI A'CCwGG_T
Cuts at: 0 2300 2583
Size: 2300 283
SfcI C'TryA_G
Cuts at: 0 1070 1519 1905 2207 2256 2330 2583
Size: 1070 449 386 302 49 74 253
Fragments arranged by size:
1070 449 386 302 253 74 49
SmlI C'TyrA_G
Cuts at: 0 991 2583
Size: 991 1592
SspI AAT'ATT
Cuts at: 0 227 901 2583
Size: 227 674 1682
Fragments arranged by size:
1682 674 227
StuI AGG'CCT
Cuts at: 0 2527 2583
Size: 2527 56
StyI C'CwwG_G
Cuts at: 0 60 2528 2583
Size: 60 2468 55
Fragments arranged by size:
2468 60 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 360 1126 1278 2583
Size: 360 766 152 1305
Fragments arranged by size:
1305 766 360 152
TatI w'GTAC_w
Cuts at: 0 269 444 886 2583
Size: 269 175 442 1697
Fragments arranged by size:
1697 442 269 175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 264 296 463 495 1598 1630 2583
Size: 264 32 167 32 1103 32 953
Fragments arranged by size:
1103 953 264 167 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1812 2583
Size: 1812 771
XhoI C'TCGA_G
Cuts at: 0 991 2583
Size: 991 1592
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AloI AlwNI ApaLI
ApoI AvaI BaeI BamHI BanII BbsI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaI
BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NheI NspI PciI PflMI PfoI PpiI
PpuMI PspXI PsrI PstI PvuII SacI SanDI SapI
SexAI SfcI SmlI SspI StuI StyI TaqII TatI
TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
ApaI AscI AseI AsiSI AvrII BanI BbeI BbvCI
BcgI BciVI BfrBI BglI BmgBI BpuEI BsaAI BsaHI
BsaWI BsiWI BsmBI BspEI BssHII BssSI BstXI BstZ17I
ClaI CspCI DrdI EciI EcoRV FalI FseI FspI
FspAI HpaI KasI KpnI MluI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI PacI PasI PmeI
PmlI PshAI PsiI PspOMI PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI Tth111I XbaI XmaI XmnI
ZraI