Restriction map for
pF1KA1296
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# NO CUT ENZYMES ( 99 enzymes) #
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Acc65I AccI AclI AflIII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BbeI BbrPI
BbvCI BciVI BlnI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI Eam1105I EcoNI
EcoO65I EcoRI EcoT22I FseI FspI
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1436 GACGTC GACGT^C
AflII 747 CTTAAG C^TTAAG
Aor51HI 1565 AGCGCT AGC^GCT
AvaI 1968 CCCGGG C^[CT]CG[GA]G
BanI 48 GGCACC G^G[CT][GA]CC
BfrI 747 CTTAAG C^TTAAG
BglI 1520 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 864 AGATCT A^GATCT
BlpI 1148 GCTCAGC GC^T[ACGT]AGC
BpmI 304 CTGGAG CTGGAG(16/14)
Bpu1102I 1148 GCTCAGC GC^T[ACGT]AGC
BsaAI 2005 TACGTG [CT]AC^GT[GA]
BsaBI 859 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1626 GAGACC GGTCTC(1/5)
BsiEI 1749 CGGCCG CG[GA][CT]^CG
BsmBI 1473 GAGACG CGTCTC(1/5)
BsoBI 1968 CCCGGG C^[CT]CG[GA]G
BspHI 1440 TCATGA T^CATGA
BsrDI 59 GCAATG GCAATG(2/0)
BstBI 1430 TTCGAA TT^CGAA
CelII 1148 GCTCAGC GC^T[ACGT]AGC
DraI 1651 TTTAAA TTT^AAA
EagI 1749 CGGCCG C^GGCCG
EclXI 1749 CGGCCG C^GGCCG
Eco47III 1565 AGCGCT AGC^GCT
Eco52I 1749 CGGCCG C^GGCCG
EcoRV 2233 GATATC GAT^ATC
HaeII 1565 AGCGCT [GA]GCGC^[CT]
HincII 1821 GTTGAC GT[CT]^[GA]AC
HindII 1821 GTTGAC GT[CT]^[GA]AC
MamI 859 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1397 CAGCTG C[AC]G^C[GT]G
NcoI 761 CCATGG C^CATGG
NspV 1430 TTCGAA TT^CGAA
PpuMI 1574 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2140 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1681 CTGCAG CTGCA^G
PvuII 1397 CAGCTG CAG^CTG
RcaI 1440 TCATGA T^CATGA
SacI 1146 GAGCTC GAGCT^C
SexAI 297 ACCAGGT A^CC[AT]GGT
SfuI 1430 TTCGAA TT^CGAA
SmaI 1968 CCCGGG CCC^GGG
StuI 153 AGGCCT AGG^CCT
XmaCI 1968 CCCGGG C^CCGGG
XmaI 1968 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccIII 785 TCCGGA T^CCGGA
1167 TCCGGA
AocI 149 CCTCAGG CC^T[ACGT]AGG
268 CCTCAGG
Asp700I 485 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1423 GAATTTATTC
BamHI 177 GGATCC G^GATCC
1964 GGATCC
BcgI 573 GCAACACAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1772 GCACCTACATCG
BclI 1719 TGATCA T^GATCA
2015 TGATCA
BfaI 157 CTAG C^TAG
1259 CTAG
BsaWI 785 TCCGGA [AT]^CCGG[AT]
1167 TCCGGA
BseAI 785 TCCGGA T^CCGGA
1167 TCCGGA
BspEI 785 TCCGGA T^CCGGA
1167 TCCGGA
BsrBI 999 CCGCTC CCGCTC(-3/-3)
206 GAGCGG
Bsu36I 149 CCTCAGG CC^T[ACGT]AGG
268 CCTCAGG
CfoI 95 GCGC GCG^C
1566 GCGC
DraII 1322 AGGGCCT [GA]G^G[ACGT]CC[CT]
1574 GGGACCT
EaeI 977 CGGCCA [CT]^GGCC[GA]
1749 CGGCCG
Eco81I 149 CCTCAGG CC^T[ACGT]AGG
268 CCTCAGG
EcoO109I 1322 AGGGCCT [GA]G^G[ACGT]CC[CT]
1574 GGGACCT
EcoT14I 392 CCAAGG C^C[AT][AT]GG
761 CCATGG
FauI 998 CCCGC CCCGC(4/6)
723 GCGGG
FbaI 1719 TGATCA T^GATCA
2015 TGATCA
HhaI 95 GCGC GCG^C
1566 GCGC
HinP1I 95 GCGC G^CGC
1566 GCGC
MaeI 157 CTAG C^TAG
1259 CTAG
MaeII 1437 ACGT A^CGT
2006 ACGT
MroI 785 TCCGGA T^CCGGA
1167 TCCGGA
MslI 123 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2001 CACCTACGTG
SapI 732 GAAGAGC GCTCTTC(1/4)
1001 GCTCTTC
StyI 392 CCAAGG C^C[AT][AT]GG
761 CCATGG
XcmI 761 CCATGGATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1997 CCATCACCTACGTGG
XmnI 485 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1423 GAATTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanII BbsI BbvI BcnI
BmyI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EarI
EcoRII Fnu4HI FokI HaeIII HapII
HgaI Hin1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII