Restriction map for pF1KA1296
 (Linear) (Six-base) MAPSORT of: KIAA1296.seq  Check: 3029  from: 1  to: 2343



KIAA1296.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AatII G_ACGT'C

Cuts at:      0    1440    2343 
   Size:       1440     903
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     914    1033    1163    1303    1687    1887    2343 
   Size:        305     609     119     130     140     384     200     456

  Fragments arranged by size: 

                 609     456     384     305     200     140     130     119
            
 
AfeI AGC'GCT

Cuts at:      0    1567    2343 
   Size:       1567     776
 
AflII C'TTAA_G

Cuts at:      0     747    2343 
   Size:        747    1596
 
AleI CACnn'nnGTG

Cuts at:      0    2005    2343 
   Size:       2005     338
 
AlwNI CAG_nnn'CTG

Cuts at:      0     619    1326    1665    2343 
   Size:        619     707     339     678

  Fragments arranged by size: 

                 707     678     619     339
 
ApoI r'AATT_y

Cuts at:      0    1172    1280    1423    1642    2343 
   Size:       1172     108     143     219     701

  Fragments arranged by size: 

                1172     701     219     143     108
 
AvaI C'yCGr_G

Cuts at:      0    1968    2343 
   Size:       1968     375
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1874    1907    2320    2343 
   Size:       1874      33     413      23

  Fragments arranged by size: 

                1874     413      33      23
 
BamHI G'GATC_C

Cuts at:      0     177    1964    2343 
   Size:        177    1787     379

  Fragments arranged by size: 

                1787     379     177
 
BanI G'GyrC_C

Cuts at:      0      48    2343 
   Size:         48    2295
 
BanII G_rGCy'C

Cuts at:      0     268    1088    1150    1245    1337    2343 
   Size:        268     820      62      95      92    1006

  Fragments arranged by size: 

                1006     820     268      95      92      62
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     139     221     902    1112    1440    2343 
   Size:        139      82     681     210     328     903

  Fragments arranged by size: 

                 903     681     328     210     139      82
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     562     596    1761    1795    2343 
   Size:        562      34    1165      34     548

  Fragments arranged by size: 

                1165     562     548      34      34
 
BclI T'GATC_A

Cuts at:      0    1719    2015    2343 
   Size:       1719     296     328

  Fragments arranged by size: 

                1719     328     296
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1526    2343 
   Size:       1526     817
 
BglII A'GATC_T

Cuts at:      0     864    2343 
   Size:        864    1479
 
BlpI GC'TnA_GC

Cuts at:      0    1149    2343 
   Size:       1149    1194
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     206    1047    2343 
   Size:        206     841    1296

  Fragments arranged by size: 

                1296     841     206
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     666    2343 
   Size:        666    1677
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325    2343 
   Size:        325    2018
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1321    2096    2343 
   Size:       1321     775     247

  Fragments arranged by size: 

                1321     775     247
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1620    2343 
   Size:       1620     723
 
BsaAI yAC'GTr

Cuts at:      0    2007    2343 
   Size:       2007     336
 
BsaBI GATnn'nnATC

Cuts at:      0     863    2343 
   Size:        863    1480
 
BsaHI Gr'CG_yC

Cuts at:      0    1437    1518    1529    2343 
   Size:       1437      81      11     814

  Fragments arranged by size: 

                1437     814      81      11
 
BsaWI w'CCGG_w

Cuts at:      0     785    1167    2343 
   Size:        785     382    1176

  Fragments arranged by size: 

                1176     785     382
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     891     921     974    1004    1682    1712    2222    2252 
   Size:        891      30      53      30     678      30     510      30

Cuts at:   2252    2343 
   Size:         91

  Fragments arranged by size: 

                 891     678     510      91      53      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1570    2058    2061    2343 
   Size:       1570     488       3     282

  Fragments arranged by size: 

                1570     488     282       3
 
BseYI C'CCAG_C

Cuts at:      0     371     993    2117    2343 
   Size:        371     622    1124     226

  Fragments arranged by size: 

                1124     622     371     226
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177    1100    1852    2343 
   Size:        177     923     752     491

  Fragments arranged by size: 

                 923     752     491     177
 
BsiEI CG_ry'CG

Cuts at:      0    1752    2343 
   Size:       1752     591
 
BsiHKAI G_wGCw'C

Cuts at:      0     569     739    1150    2343 
   Size:        569     170     411    1193

  Fragments arranged by size: 

                1193     569     411     170
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1467    2343 
   Size:       1467     876
 
Bsp1286I G_dGCh'C

Cuts at:      0     268     569     739    1088    1150    1245    1337    2343 
   Size:        268     301     170     349      62      95      92    1006

  Fragments arranged by size: 

                1006     349     301     268     170      95      92      62
            
 
BspEI T'CCGG_A

Cuts at:      0     785    1167    2343 
   Size:        785     382    1176

  Fragments arranged by size: 

                1176     785     382
 
BspHI T'CATG_A

Cuts at:      0    1440    2343 
   Size:       1440     903
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1638    1824    1926    2066    2343 
   Size:       1638     186     102     140     277

  Fragments arranged by size: 

                1638     277     186     140     102
 
BsrBI CCG'CTC

Cuts at:      0     208    1001    2343 
   Size:        208     793    1342

  Fragments arranged by size: 

                1342     793     208
 
BsrDI GCAATG_nn'

Cuts at:      0      66    2343 
   Size:         66    2277
 
BstBI TT'CG_AA

Cuts at:      0    1431    2343 
   Size:       1431     912
 
BstYI r'GATC_y

Cuts at:      0     177     615     833     864    1251    1964    2343 
   Size:        177     438     218      31     387     713     379

  Fragments arranged by size: 

                 713     438     387     379     218     177      31
 
Bsu36I CC'TnA_GG

Cuts at:      0     150     269    2343 
   Size:        150     119    2074

  Fragments arranged by size: 

                2074     150     119
 
BtgI C'CryG_G

Cuts at:      0     761    1415    1746    2343 
   Size:        761     654     331     597

  Fragments arranged by size: 

                 761     654     597     331
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     767    2240    2343 
   Size:        767    1473     103

  Fragments arranged by size: 

                1473     767     103
 
BtsI GCAGTG_nn'

Cuts at:      0    1226    1924    2090    2091    2343 
   Size:       1226     698     166       1     252

  Fragments arranged by size: 

                1226     698     252     166       1
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     528     563    2343 
   Size:        528      35    1780

  Fragments arranged by size: 

                1780     528      35
 
DraI TTT'AAA

Cuts at:      0    1653    2343 
   Size:       1653     690
 
EaeI y'GGCC_r

Cuts at:      0     977    1749    2343 
   Size:        977     772     594

  Fragments arranged by size: 

                 977     772     594
 
EagI C'GGCC_G

Cuts at:      0    1749    2343 
   Size:       1749     594
 
EarI CTCTTCn'nnn_

Cuts at:      0     384     627     727    1008    1219    1322    1490    2121 
   Size:        384     243     100     281     211     103     168     631

Cuts at:   2121    2343 
   Size:        222

  Fragments arranged by size: 

                 631     384     281     243     222     211     168     103
                 100
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     516    2343 
   Size:        516    1827
 
EcoICRI GAG'CTC

Cuts at:      0    1148    2343 
   Size:       1148    1195
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     325     914    1033    1163    1303    1687    1887 
   Size:        305      20     589     119     130     140     384     200

Cuts at:   1887    2343 
   Size:        456

  Fragments arranged by size: 

                 589     456     384     305     200     140     130     119
                  20
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1323    1575    2343 
   Size:       1323     252     768

  Fragments arranged by size: 

                1323     768     252
 
EcoRV GAT'ATC

Cuts at:      0    2235    2343 
   Size:       2235     108
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1474    1887    2343 
   Size:       1474     413     456

  Fragments arranged by size: 

                1474     456     413
 
HaeII r_GCGC'y

Cuts at:      0    1569    2343 
   Size:       1569     774
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     537     569     607     639     856     888     889     921 
   Size:        537      32      38      32     217      32       1      32

Cuts at:    921    1360    1392    1530    1562    1973    2005    2343 
   Size:        439      32     138      32     411      32     338

  Fragments arranged by size: 

                 537     439     411     338     217     138      38      32
                  32      32      32      32      32      32       1
 
HincII GTy'rAC

Cuts at:      0    1823    2343 
   Size:       1823     520
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1232    1561    2195    2343 
   Size:       1232     329     634     148

  Fragments arranged by size: 

                1232     634     329     148
 
MslI CAynn'nnrTG

Cuts at:      0     127    2005    2343 
   Size:        127    1878     338

  Fragments arranged by size: 

                1878     338     127
 
MspA1I CmG'CkG

Cuts at:      0    1399    2343 
   Size:       1399     944
 
NcoI C'CATG_G

Cuts at:      0     761    2343 
   Size:        761    1582
 
PfoI T'CCnGG_A

Cuts at:      0    1188    2343 
   Size:       1188    1155
 
PpuMI rG'GwC_Cy

Cuts at:      0    1575    2343 
   Size:       1575     768
 
PshAI GACnn'nnGTC

Cuts at:      0    2144    2343 
   Size:       2144     199
 
PsiI TTA'TAA

Cuts at:      0    1286    1709    2343 
   Size:       1286     423     634

  Fragments arranged by size: 

                1286     634     423
 
PstI C_TGCA'G

Cuts at:      0    1685    2343 
   Size:       1685     658
 
PvuII CAG'CTG

Cuts at:      0    1399    2343 
   Size:       1399     944
 
SacI G_AGCT'C

Cuts at:      0    1150    2343 
   Size:       1150    1193
 
SapI GCTCTTCn'nnn_

Cuts at:      0     727    1008    2343 
   Size:        727     281    1335

  Fragments arranged by size: 

                1335     727     281
 
SexAI A'CCwGG_T

Cuts at:      0     297    2343 
   Size:        297    2046
 
SfcI C'TryA_G

Cuts at:      0     167     234     399    1681    2343 
   Size:        167      67     165    1282     662

  Fragments arranged by size: 

                1282     662     167     165      67
 
SmaI CCC'GGG

Cuts at:      0    1970    2343 
   Size:       1970     373
 
SmlI C'TyrA_G

Cuts at:      0     747    1336    2111    2343 
   Size:        747     589     775     232

  Fragments arranged by size: 

                 775     747     589     232
 
StuI AGG'CCT

Cuts at:      0     155    2343 
   Size:        155    2188
 
StyI C'CwwG_G

Cuts at:      0     392     761    2343 
   Size:        392     369    1582

  Fragments arranged by size: 

                1582     392     369
 
TatI w'GTAC_w

Cuts at:      0     869    2343 
   Size:        869    1474
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     891     923     974    1006    2343 
   Size:        891      32      51      32    1337

  Fragments arranged by size: 

                1337     891      51      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     768    2004    2343 
   Size:        768    1236     339

  Fragments arranged by size: 

                1236     768     339
 
XmaI C'CCGG_G

Cuts at:      0    1968    2343 
   Size:       1968     375
 
XmnI GAAnn'nnTTC

Cuts at:      0     489    1427    2343 
   Size:        489     938     916

  Fragments arranged by size: 

                 938     916     489
 
ZraI GAC'GTC

Cuts at:      0    1438    2343 
   Size:       1438     905
 

 Enzymes that do cut:

    AatII     AcuI     AfeI    AflII     AleI    AlwNI     ApoI     AvaI
     BaeI    BamHI     BanI    BanII     BbsI     BcgI     BclI     BglI
    BglII     BlpI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRV
     FalI    HaeII    Hin4I   HincII     MmeI     MslI   MspA1I     NcoI
     PfoI    PpuMI    PshAI     PsiI     PstI    PvuII     SacI     SapI
    SexAI     SfcI     SmaI     SmlI     StuI     StyI     TatI     TstI
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI   AflIII     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BbeI
    BbvCI    BciVI    BfrBI Bme1580I    BmgBI     BmtI   Bpu10I    BsiWI
     BsmI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
  BstZ17I     ClaI   DraIII     DrdI    EcoNI    EcoRI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI     XhoI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com