Restriction map for pF1KA1271
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BsaBI       BseAI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      ClaI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfuI        SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccII         252  CGCG                 CG^CG
AcsI          465  GAATTC               [GA]^AATT[CT]
ApoI          465  GAATTC               [GA]^AATT[CT]
AspHI        1102  GTGCTC               G[AT]GC[AT]^C
AvaI         1296  CTCGGG               C^[CT]CG[GA]G
BamHI        1400  GGATCC               G^GATCC
BbrPI        1405  CACGTG               CAC^GTG
BbsI           13  GAAGAC               GAAGAC(2/6)
BbvCI         558  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1308  CGAGGATCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI          13  GAAGAC               GAAGAC(2/6)
BsaAI        1405  CACGTG               [CT]AC^GT[GA]
BsaWI        1493  ACCGGA               [AT]^CCGG[AT]
BsiEI        1491  CGACCG               CG[GA][CT]^CG
BsiHKAI      1102  GTGCTC               G[AT]GC[AT]^C
BsoBI        1296  CTCGGG               C^[CT]CG[GA]G
BstUI         252  CGCG                 CG^CG
CpoI          299  CGGACCG              CG^G[AT]CCG
EarI          172  CTCTTC               CTCTTC(1/4)
EcoRI         465  GAATTC               G^AATTC
Ksp632I       172  CTCTTC               CTCTTC(1/4)
MaeII        1406  ACGT                 A^CGT
MvnI          252  CGCG                 CG^CG
NaeI         1183  GCCGGC               GCC^GGC
NcoI         1356  CCATGG               C^CATGG
NgoMIV       1183  GCCGGC               G^CCGGC
PmaCI        1405  CACGTG               CAC^GTG
PmlI         1405  CACGTG               CAC^GTG
PvuII         393  CAGCTG               CAG^CTG
RsrII         299  CGGACCG              CG^G[AT]CCG
ScaI          209  AGTACT               AGT^ACT
SgrAI        1182  CGCCGGCG             C[GA]^CCGG[CT]G
XcmI         1328  CCAGCACCTCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          271  GTCTAC               GT^[AC][GT]AC
             1282  GTAGAC              
ApaI          352  GGGCCC               GGGCC^C
             1348  GGGCCC              
BbeI          444  GGCGCC               GGCGC^C
             1186  GGCGCC              
BlpI          489  GCTCAGC              GC^T[ACGT]AGC
             1202  GCTCAGC             
Bpu1102I      489  GCTCAGC              GC^T[ACGT]AGC
             1202  GCTCAGC             
BsrDI         216  CATTGC               GCAATG(2/0)
               59  GCAATG              
BsrFI        1183  GCCGGC               [GA]^CCGG[CT]
             1604  ACCGGC              
BstYI        1311  GGATCT               [GA]^GATC[CT]
             1400  GGATCC              
CelII         489  GCTCAGC              GC^T[ACGT]AGC
             1202  GCTCAGC             
Cfr10I       1183  GCCGGC               [GA]^CCGG[CT]
             1604  ACCGGC              
DsaI          651  CCGTGG               C^C[GA][CT]GG
             1356  CCATGG              
HaeII         444  GGCGCC               [GA]GCGC^[CT]
             1186  GGCGCC              
HgaI          486  GACGC                GACGC(5/10)
             1611  GCGTC               
KasI          444  GGCGCC               G^GCGCC
             1186  GGCGCC              
MboII          13  GAAGA                GAAGA(8/7)
              173  TCTTC               
MflI         1311  GGATCT               [GA]^GATC[CT]
             1400  GGATCC              
MslI          900  CACCTTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1107  CACCAAGGTG          
NarI          444  GGCGCC               GG^CGCC
             1186  GGCGCC              
PleI          451  GAGTC                GAGTC(4/5)
              535  GAGTC               
SfoI          444  GGCGCC               GGC^GCC
             1186  GGCGCC              
TaqI          120  TCGA                 T^CGA
             1307  TCGA                
TfiI           78  GATTC                G^A[AT]TC
              513  GAATC               
TthHB8I       120  TCGA                 T^CGA
             1307  TCGA                
XhoII        1311  GGATCT               [GA]^GATC[CT]
             1400  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AvaII       BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TseI        Tsp45I      Tsp509I     
TspRI       Van91I      
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