Restriction map for
pF1KA1271
(Linear) (Six-base) MAPSORT of: KIAA1271.seq Check: 498 from: 1 to: 1620
KIAA1271.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 142 372 613 876 1464 1620
Size: 142 230 241 263 588 156
Fragments arranged by size:
588 263 241 230 156 142
AccI GT'mk_AC
Cuts at: 0 272 1283 1620
Size: 272 1011 337
Fragments arranged by size:
1011 337 272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 169 1620
Size: 32 137 1451
Fragments arranged by size:
1451 137 32
AleI CACnn'nnGTG
Cuts at: 0 1111 1620
Size: 1111 509
AlwNI CAG_nnn'CTG
Cuts at: 0 687 796 952 1434 1620
Size: 687 109 156 482 186
Fragments arranged by size:
687 482 186 156 109
ApaI G_GGCC'C
Cuts at: 0 356 1352 1620
Size: 356 996 268
Fragments arranged by size:
996 356 268
ApoI r'AATT_y
Cuts at: 0 465 1620
Size: 465 1155
AvaI C'yCGr_G
Cuts at: 0 1296 1620
Size: 1296 324
BamHI G'GATC_C
Cuts at: 0 1400 1620
Size: 1400 220
BanI G'GyrC_C
Cuts at: 0 444 751 864 1074 1186 1390 1515 1620
Size: 444 307 113 210 112 204 125 105
Fragments arranged by size:
444 307 210 204 125 113 112 105
BanII G_rGCy'C
Cuts at: 0 356 1352 1545 1620
Size: 356 996 193 75
Fragments arranged by size:
996 356 193 75
BbeI G_GCGC'C
Cuts at: 0 448 1190 1620
Size: 448 742 430
Fragments arranged by size:
742 448 430
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 1620
Size: 20 1600
BbvCI CC'TCA_GC
Cuts at: 0 559 1620
Size: 559 1061
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1297 1331 1620
Size: 1297 34 289
Fragments arranged by size:
1297 289 34
BlpI GC'TnA_GC
Cuts at: 0 490 1203 1620
Size: 490 713 417
Fragments arranged by size:
713 490 417
Bme1580I G_kGCm'C
Cuts at: 0 356 968 1352 1393 1620
Size: 356 612 384 41 227
Fragments arranged by size:
612 384 356 227 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 613 1620
Size: 613 1007
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 558 1620
Size: 558 1062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 553 558 618 834 1214 1456 1537
Size: 340 213 5 60 216 380 242 81
Cuts at: 1537 1620
Size: 83
Fragments arranged by size:
380 340 242 216 213 83 81 60
5
Bpu10I CC'TnA_GC
Cuts at: 0 559 1620
Size: 559 1061
BsaI GGTCTCn'nnnn_
Cuts at: 0 104 430 1153 1620
Size: 104 326 723 467
Fragments arranged by size:
723 467 326 104
BsaAI yAC'GTr
Cuts at: 0 1407 1620
Size: 1407 213
BsaHI Gr'CG_yC
Cuts at: 0 445 1187 1611 1620
Size: 445 742 424 9
Fragments arranged by size:
742 445 424 9
BsaWI w'CCGG_w
Cuts at: 0 1493 1620
Size: 1493 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 313 343 1360 1390 1620
Size: 313 30 1017 30 230
Fragments arranged by size:
1017 313 230 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 531 753 756 1171 1381 1620
Size: 531 222 3 415 210 239
Fragments arranged by size:
531 415 239 222 210 3
BseYI C'CCAG_C
Cuts at: 0 200 945 1164 1420 1620
Size: 200 745 219 256 200
Fragments arranged by size:
745 256 219 200 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 836 1022 1600 1620
Size: 120 716 186 578 20
Fragments arranged by size:
716 578 186 120 20
BsiEI CG_ry'CG
Cuts at: 0 1494 1620
Size: 1494 126
BsiHKAI G_wGCw'C
Cuts at: 0 1106 1620
Size: 1106 514
Bsp1286I G_dGCh'C
Cuts at: 0 356 968 1106 1352 1393 1545 1620
Size: 356 612 138 246 41 152 75
Fragments arranged by size:
612 356 246 152 138 75 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 142 372 613 876 1464 1620
Size: 98 44 230 241 263 588 156
Fragments arranged by size:
588 263 241 230 156 98 44
BsrDI GCAATG_nn'
Cuts at: 0 66 215 1620
Size: 66 149 1405
Fragments arranged by size:
1405 149 66
BsrFI r'CCGG_y
Cuts at: 0 1183 1604 1620
Size: 1183 421 16
Fragments arranged by size:
1183 421 16
BstAPI GCAn_nnn'nTGC
Cuts at: 0 56 480 796 1620
Size: 56 424 316 824
Fragments arranged by size:
824 424 316 56
BstYI r'GATC_y
Cuts at: 0 1311 1400 1620
Size: 1311 89 220
Fragments arranged by size:
1311 220 89
BtgI C'CryG_G
Cuts at: 0 651 1356 1620
Size: 651 705 264
Fragments arranged by size:
705 651 264
BtsI GCAGTG_nn'
Cuts at: 0 1010 1620
Size: 1010 610
EarI CTCTTCn'nnn_
Cuts at: 0 178 1620
Size: 178 1442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 169 340 553 558 618 834 1214
Size: 32 137 171 213 5 60 216 380
Cuts at: 1214 1456 1537 1620
Size: 242 81 83
Fragments arranged by size:
380 242 216 213 171 137 83 81
60 32 5
EcoO109I rG'GnC_Cy
Cuts at: 0 352 353 1236 1348 1349 1529 1620
Size: 352 1 883 112 1 180 91
Fragments arranged by size:
883 352 180 112 91 1 1
EcoRI G'AATT_C
Cuts at: 0 465 1620
Size: 465 1155
HaeII r_GCGC'y
Cuts at: 0 448 1190 1620
Size: 448 742 430
Fragments arranged by size:
742 448 430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 1207 1239 1366 1398 1620
Size: 313 32 862 32 127 32 222
Fragments arranged by size:
862 313 222 127 32 32 32
KasI G'GCGC_C
Cuts at: 0 444 1186 1620
Size: 444 742 434
Fragments arranged by size:
742 444 434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1198 1226 1620
Size: 1198 28 394
Fragments arranged by size:
1198 394 28
MslI CAynn'nnrTG
Cuts at: 0 904 1111 1620
Size: 904 207 509
Fragments arranged by size:
904 509 207
MspA1I CmG'CkG
Cuts at: 0 189 395 578 1620
Size: 189 206 183 1042
Fragments arranged by size:
1042 206 189 183
NaeI GCC'GGC
Cuts at: 0 1185 1620
Size: 1185 435
NarI GG'CG_CC
Cuts at: 0 445 1187 1620
Size: 445 742 433
Fragments arranged by size:
742 445 433
NcoI C'CATG_G
Cuts at: 0 1356 1620
Size: 1356 264
NgoMIV G'CCGG_C
Cuts at: 0 1183 1620
Size: 1183 437
PasI CC'CwG_GG
Cuts at: 0 704 1447 1620
Size: 704 743 173
Fragments arranged by size:
743 704 173
PflMI CCAn_nnn'nTGG
Cuts at: 0 523 652 687 1434 1620
Size: 523 129 35 747 186
Fragments arranged by size:
747 523 186 129 35
PfoI T'CCnGG_A
Cuts at: 0 431 1433 1502 1620
Size: 431 1002 69 118
Fragments arranged by size:
1002 431 118 69
PmlI CAC'GTG
Cuts at: 0 1407 1620
Size: 1407 213
PspOMI G'GGCC_C
Cuts at: 0 352 1348 1620
Size: 352 996 272
Fragments arranged by size:
996 352 272
PstI C_TGCA'G
Cuts at: 0 400 485 792 801 1620
Size: 400 85 307 9 819
Fragments arranged by size:
819 400 307 85 9
PvuII CAG'CTG
Cuts at: 0 395 1620
Size: 395 1225
RsrII CG'GwC_CG
Cuts at: 0 300 1620
Size: 300 1320
ScaI AGT'ACT
Cuts at: 0 211 1620
Size: 211 1409
SfcI C'TryA_G
Cuts at: 0 396 481 788 797 1620
Size: 396 85 307 9 823
Fragments arranged by size:
823 396 307 85 9
SfoI GGC'GCC
Cuts at: 0 446 1188 1620
Size: 446 742 432
Fragments arranged by size:
742 446 432
SgrAI Cr'CCGG_yG
Cuts at: 0 1183 1620
Size: 1183 437
StyI C'CwwG_G
Cuts at: 0 508 1109 1239 1337 1356 1620
Size: 508 601 130 98 19 264
Fragments arranged by size:
601 508 264 130 98 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 192 1620
Size: 192 1428
TatI w'GTAC_w
Cuts at: 0 209 1620
Size: 209 1411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1335 1620
Size: 1335 285
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BstAPI BstYI BtgI BtsI EarI
Eco57MI EcoO109I EcoRI HaeII Hin4I KasI MmeI MslI
MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI
PmlI PspOMI PstI PvuII RsrII ScaI SfcI SfoI
SgrAI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BciVI BclI BfrBI BglI BglII BmgBI BmtI BpuEI
BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI