Restriction map for
pF1KA1226
(Circular) (Six-base) MAPSORT of: pF1KA1226.seq Check: 321 from: 1 to: 5225
pF1KA1226 5225 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 1724 1724
Size: 5225
AccI GT'mk_AC
Cuts at: 2263 2263
Size: 5225
Acc65I G'GTAC_C
Cuts at: 2241 2241
Size: 5225
AclI AA'CG_TT
Cuts at: 5065 5065
Size: 5225
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1298 3050 3482 3835 4497 193
Size: 1105 1752 432 353 662 921
Fragments arranged by size:
1752 1105 921 662 432 353
AflIII A'CryG_T
Cuts at: 2577 4377 4658 2577
Size: 1800 281 3144
Fragments arranged by size:
3144 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1975 2164 1975
Size: 189 5036
AlwNI CAG_nnn'CTG
Cuts at: 381 1516 2530 3968 381
Size: 1135 1014 1438 1638
Fragments arranged by size:
1638 1438 1135 1014
ApaLI G'TGCA_C
Cuts at: 1628 4063 1628
Size: 2435 2790
ApoI r'AATT_y
Cuts at: 1122 2229 4578 4834 1122
Size: 1107 2349 256 1513
Fragments arranged by size:
2349 1513 1107 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5225
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5225
AvaI C'yCGr_G
Cuts at: 2245 2245
Size: 5225
BamHI G'GATC_C
Cuts at: 2250 2250
Size: 5225
BanI G'GyrC_C
Cuts at: 119 1686 2241 2655 2903 2938 119
Size: 1567 555 414 248 35 2406
Fragments arranged by size:
2406 1567 555 414 248 35
BanII G_rGCy'C
Cuts at: 299 641 2239 3269 299
Size: 342 1598 1030 2255
Fragments arranged by size:
2255 1598 1030 342
BbeI G_GCGC'C
Cuts at: 2659 2907 2659
Size: 248 4977
BbsI GAAGACnn'nnnn_
Cuts at: 212 1816 212
Size: 1604 3621
BciVI GTATCCnnnnn_n'
Cuts at: 453 886 1984 3123 4179 5225 453
Size: 433 1098 1139 1056 1046 453
Fragments arranged by size:
1139 1098 1056 1046 453 433
BclI T'GATC_A
Cuts at: 1860 2745 1860
Size: 885 4340
BfrBI ATG'CAT
Cuts at: 1617 1617
Size: 5225
BglII A'GATC_T
Cuts at: 2740 4777 2740
Size: 2037 3188
BlpI GC'TnA_GC
Cuts at: 2337 2337
Size: 5225
Bme1580I G_kGCm'C
Cuts at: 1632 2850 2943 4067 1632
Size: 1218 93 1124 2790
Fragments arranged by size:
2790 1218 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2437 2518 2853 4997 2437
Size: 81 335 2144 2665
Fragments arranged by size:
2665 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3592 3592
Size: 5225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1453 1931 1453
Size: 478 4747
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 1231 2401 3280 3994 4292 1204
Size: 27 1170 879 714 298 2137
Fragments arranged by size:
2137 1170 879 714 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 1471 4929 1471
Size: 3458 1767
BsaAI yAC'GTr
Cuts at: 1611 2578 1611
Size: 967 4258
BsaBI GATnn'nnATC
Cuts at: 6 2739 4776 6
Size: 2733 2037 455
Fragments arranged by size:
2733 2037 455
BsaHI Gr'CG_yC
Cuts at: 1721 2656 2904 3606 5102 1721
Size: 935 248 702 1496 1844
Fragments arranged by size:
1844 1496 935 702 248
BsaWI w'CCGG_w
Cuts at: 2217 2414 2935 3613 4024 4171 4445 2217
Size: 197 521 678 411 147 274 2997
Fragments arranged by size:
2997 678 521 411 274 197 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 194 307 539 194
Size: 113 232 4880
Fragments arranged by size:
4880 232 113
BseYI C'CCAG_C
Cuts at: 434 2650 3408 4073 4481 434
Size: 2216 758 665 408 1178
Fragments arranged by size:
2216 1178 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 325
Size: 5225
BsiEI CG_ry'CG
Cuts at: 104 2767 2813 4043 104
Size: 2663 46 1230 1286
Fragments arranged by size:
2663 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 299 774 1042 1632 2239 3017 3207 4067 299
Size: 475 268 590 607 778 190 860 1457
Fragments arranged by size:
1457 860 778 607 590 475 268 190
BsmI GAATG_Cn'
Cuts at: 341 1144 341
Size: 803 4422
Bsp1286I G_dGCh'C
Cuts at: 299 641 774 1042 1632 2239 2850 2943 3017
Size: 342 133 268 590 607 611 93 74
Cuts at: 3017 3207 3269 4067 299
Size: 190 62 798 1457
Fragments arranged by size:
1457 798 611 607 590 342 268 190
133 93 74 62
BspHI T'CATG_A
Cuts at: 860 1596 3657 860
Size: 736 2061 2428
Fragments arranged by size:
2428 2061 736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1540 2275 2489 2791 3172 1540
Size: 735 214 302 381 3593
Fragments arranged by size:
3593 735 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3517 3571 5055 5220 50
Size: 3467 54 1484 165 55
Fragments arranged by size:
3467 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 221 3137 221
Size: 2916 2309
BsrFI r'CCGG_y
Cuts at: 3223 3404 3223
Size: 181 5044
BssSI C'ACGA_G
Cuts at: 1522 3496 4204 1522
Size: 1974 708 2543
Fragments arranged by size:
2543 1974 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1516 2602 1516
Size: 1086 4139
BstBI TT'CG_AA
Cuts at: 3586 3586
Size: 5225
BstXI CCAn_nnnn'nTGG
Cuts at: 2008 2008
Size: 5225
BstYI r'GATC_y
Cuts at: 1 258 1185 2128 2250 2717 2740 3075 3321
Size: 257 927 943 122 467 23 335 246
Cuts at: 3321 3627 3639 3725 4777 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 943 927 467 449 335 306 257
246 122 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 587 587
Size: 5225
BtgI C'CryG_G
Cuts at: 2036 2036
Size: 5225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1676 1868 3175 3356 3519 1676
Size: 192 1307 181 163 3382
Fragments arranged by size:
3382 1307 192 181 163
BtsI GCAGTG_nn'
Cuts at: 961 1028 1517 2468 961
Size: 67 489 951 3718
Fragments arranged by size:
3718 951 489 67
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1250 1285 1250
Size: 35 5190
DraI TTT'AAA
Cuts at: 2061 2224 2061
Size: 163 5062
DrdI GACnn_nn'nnGTC
Cuts at: 2931 4275 2931
Size: 1344 3881
EaeI y'GGCC_r
Cuts at: 977 1998 2810 2984 3375 3402 4747 977
Size: 1021 812 174 391 27 1345 1455
Fragments arranged by size:
1455 1345 1021 812 391 174 27
EagI C'GGCC_G
Cuts at: 2810 2810
Size: 5225
EarI CTCTTCn'nnn_
Cuts at: 183 212 779 1874 3248 3458 183
Size: 29 567 1095 1374 210 1950
Fragments arranged by size:
1950 1374 1095 567 210 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3433 4175 4321 5035 3433
Size: 742 146 714 3623
Fragments arranged by size:
3623 742 714 146
EcoICRI GAG'CTC
Cuts at: 297 2237 297
Size: 1940 3285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1298 1453 1931 3050 3482 3835 4497 193
Size: 1105 155 478 1119 432 353 662 921
Fragments arranged by size:
1119 1105 921 662 478 432 353 155
EcoNI CCTnn'n_nnAGG
Cuts at: 727 727
Size: 5225
EcoO109I rG'GnC_Cy
Cuts at: 2364 2364
Size: 5225
EcoRI G'AATT_C
Cuts at: 2229 2229
Size: 5225
EcoRV GAT'ATC
Cuts at: 2014 2014
Size: 5225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1272 3244 1272
Size: 1972 3253
FspI TGC'GCA
Cuts at: 3006 3006
Size: 5225
HaeII r_GCGC'y
Cuts at: 2659 2907 4137 4908 2659
Size: 248 1230 771 2976
Fragments arranged by size:
2976 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 189 221 1323 1355 1675 1707 2064 2096 3067
Size: 32 1102 32 320 32 357 32 971
Cuts at: 3067 3097 3099 3129 189
Size: 30 2 30 2285
Fragments arranged by size:
2285 1102 971 357 320 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1815 2264 1815
Size: 449 4776
KasI G'GCGC_C
Cuts at: 2655 2903 2655
Size: 248 4977
KpnI G_GTAC'C
Cuts at: 2245 2245
Size: 5225
MfeI C'AATT_G
Cuts at: 1262 1262
Size: 5225
MluI A'CGCG_T
Cuts at: 4658 4658
Size: 5225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 312 2070 3984 4168 312
Size: 1758 1914 184 1369
Fragments arranged by size:
1914 1758 1369 184
MscI TGG'CCA
Cuts at: 979 2000 2986 979
Size: 1021 986 3218
Fragments arranged by size:
3218 1021 986
MslI CAynn'nnrTG
Cuts at: 1957 2041 2146 3214 3341 1957
Size: 84 105 1068 127 3841
Fragments arranged by size:
3841 1068 127 105 84
MspA1I CmG'CkG
Cuts at: 2054 2336 2650 3010 3794 4039 2054
Size: 282 314 360 784 245 3240
Fragments arranged by size:
3240 784 360 314 282 245
NaeI GCC'GGC
Cuts at: 3406 3406
Size: 5225
NarI GG'CG_CC
Cuts at: 2656 2904 2656
Size: 248 4977
NcoI C'CATG_G
Cuts at: 2036 2036
Size: 5225
NgoMIV G'CCGG_C
Cuts at: 3404 3404
Size: 5225
NsiI A_TGCA'T
Cuts at: 1619 1619
Size: 5225
NspI r_CATG'y
Cuts at: 442 1006 2151 2215 2278 2778 4381 442
Size: 564 1145 64 63 500 1603 1286
Fragments arranged by size:
1603 1286 1145 564 500 64 63
PciI A'CATG_T
Cuts at: 4377 4377
Size: 5225
PflMI CCAn_nnn'nTGG
Cuts at: 2089 2089
Size: 5225
PfoI T'CCnGG_A
Cuts at: 160 160
Size: 5225
PmeI GTTT'AAAC
Cuts at: 2224 2224
Size: 5225
PmlI CAC'GTG
Cuts at: 1611 1611
Size: 5225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 3671
Size: 32 5193
PsiI TTA'TAA
Cuts at: 1754 1754
Size: 5225
PstI C_TGCA'G
Cuts at: 377 2272 2957 377
Size: 1895 685 2645
Fragments arranged by size:
2645 1895 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5225
PvuII CAG'CTG
Cuts at: 2054 2650 3010 2054
Size: 596 360 4269
Fragments arranged by size:
4269 596 360
RsrII CG'GwC_CG
Cuts at: 3420 3420
Size: 5225
SacI G_AGCT'C
Cuts at: 299 2239 299
Size: 1940 3285
SalI G'TCGA_C
Cuts at: 2262 2262
Size: 5225
SapI GCTCTTCn'nnn_
Cuts at: 779 3248 3458 779
Size: 2469 210 2546
Fragments arranged by size:
2546 2469 210
SbfI CC_TGCA'GG
Cuts at: 2272 2272
Size: 5225
ScaI AGT'ACT
Cuts at: 4742 4742
Size: 5225
SfcI C'TryA_G
Cuts at: 33 373 1388 2268 2953 3921 4112 33
Size: 340 1015 880 685 968 191 1146
Fragments arranged by size:
1146 1015 968 880 685 340 191
SfoI GGC'GCC
Cuts at: 2657 2905 2657
Size: 248 4977
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5225
SmaI CCC'GGG
Cuts at: 2247 2247
Size: 5225
SmlI C'TyrA_G
Cuts at: 1219 1246 2380 3295 4009 4271 1219
Size: 27 1134 915 714 262 2173
Fragments arranged by size:
2173 1134 915 714 262 27
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5225
SphI G_CATG'C
Cuts at: 2215 2278 2778 2215
Size: 63 500 4662
Fragments arranged by size:
4662 500 63
SspI AAT'ATT
Cuts at: 1928 2005 1928
Size: 77 5148
StuI AGG'CCT
Cuts at: 2207 2207
Size: 5225
StyI C'CwwG_G
Cuts at: 755 971 1091 1429 2036 2359 2713 755
Size: 216 120 338 607 323 354 3267
Fragments arranged by size:
3267 607 354 338 323 216 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1084 1453 1701 2808 2941 3400 3609 1084
Size: 369 248 1107 133 459 209 2700
Fragments arranged by size:
2700 1107 459 369 248 209 133
TatI w'GTAC_w
Cuts at: 472 657 4740 472
Size: 185 4083 957
Fragments arranged by size:
4083 957 185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 179 211 836 868 1341 1373 1649 1681 4742
Size: 32 625 32 473 32 276 32 3061
Cuts at: 4742 4774 179
Size: 32 630
Fragments arranged by size:
3061 630 625 473 276 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1012 3022 1012
Size: 2010 3215
XbaI T'CTAG_A
Cuts at: 1062 2256 1062
Size: 1194 4031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 600 894 1884 2720 600
Size: 294 990 836 3105
Fragments arranged by size:
3105 990 836 294
XmaI C'CCGG_G
Cuts at: 2245 2245
Size: 5225
ZraI GAC'GTC
Cuts at: 1722 1722
Size: 5225
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I ClaI DraIII FseI FspAI HindIII HpaI NdeI
NheI NotI NruI PacI PasI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI