Restriction map for
pF1KA1226
(Linear) (Six-base) MAPSORT of: KIAA1226.seq Check: 6590 from: 1 to: 2112
KIAA1226.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AatII G_ACGT'C
Cuts at: 0 1616 2112
Size: 1616 496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 1190 2112
Size: 85 1105 922
Fragments arranged by size:
1105 922 85
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1867 2056 2112
Size: 1867 189 56
Fragments arranged by size:
1867 189 56
AlwNI CAG_nnn'CTG
Cuts at: 0 273 1408 2112
Size: 273 1135 704
Fragments arranged by size:
1135 704 273
ApaLI G'TGCA_C
Cuts at: 0 1520 2112
Size: 1520 592
ApoI r'AATT_y
Cuts at: 0 1014 2112
Size: 1014 1098
BanI G'GyrC_C
Cuts at: 0 11 1578 2112
Size: 11 1567 534
Fragments arranged by size:
1567 534 11
BanII G_rGCy'C
Cuts at: 0 191 533 2112
Size: 191 342 1579
Fragments arranged by size:
1579 342 191
BbsI GAAGACnn'nnnn_
Cuts at: 0 104 1708 2112
Size: 104 1604 404
Fragments arranged by size:
1604 404 104
BciVI GTATCCnnnnn_n'
Cuts at: 0 345 778 1876 2112
Size: 345 433 1098 236
Fragments arranged by size:
1098 433 345 236
BclI T'GATC_A
Cuts at: 0 1752 2112
Size: 1752 360
BfrBI ATG'CAT
Cuts at: 0 1509 2112
Size: 1509 603
Bme1580I G_kGCm'C
Cuts at: 0 1524 2112
Size: 1524 588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 1823 2112
Size: 1345 478 289
Fragments arranged by size:
1345 478 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1123 2112
Size: 1096 27 989
Fragments arranged by size:
1096 989 27
BsaI GGTCTCn'nnnn_
Cuts at: 0 1363 2112
Size: 1363 749
BsaAI yAC'GTr
Cuts at: 0 1503 2112
Size: 1503 609
BsaHI Gr'CG_yC
Cuts at: 0 1613 2112
Size: 1613 499
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 86 199 431 2112
Size: 86 113 232 1681
Fragments arranged by size:
1681 232 113 86
BseYI C'CCAG_C
Cuts at: 0 326 2112
Size: 326 1786
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 2112
Size: 217 1895
BsiHKAI G_wGCw'C
Cuts at: 0 191 666 934 1524 2112
Size: 191 475 268 590 588
Fragments arranged by size:
590 588 475 268 191
BsmI GAATG_Cn'
Cuts at: 0 233 1036 2112
Size: 233 803 1076
Fragments arranged by size:
1076 803 233
Bsp1286I G_dGCh'C
Cuts at: 0 191 533 666 934 1524 2112
Size: 191 342 133 268 590 588
Fragments arranged by size:
590 588 342 268 191 133
BspHI T'CATG_A
Cuts at: 0 752 1488 2112
Size: 752 736 624
Fragments arranged by size:
752 736 624
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1432 2112
Size: 1432 680
BsrDI GCAATG_nn'
Cuts at: 0 113 2112
Size: 113 1999
BssSI C'ACGA_G
Cuts at: 0 1414 2112
Size: 1414 698
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1408 2112
Size: 1408 704
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1900 2112
Size: 1900 212
BstYI r'GATC_y
Cuts at: 0 150 1077 2020 2112
Size: 150 927 943 92
Fragments arranged by size:
943 927 150 92
Bsu36I CC'TnA_GG
Cuts at: 0 479 2112
Size: 479 1633
BtgI C'CryG_G
Cuts at: 0 1928 2112
Size: 1928 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1568 1760 2112
Size: 1568 192 352
Fragments arranged by size:
1568 352 192
BtsI GCAGTG_nn'
Cuts at: 0 853 920 1409 2112
Size: 853 67 489 703
Fragments arranged by size:
853 703 489 67
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1142 1177 2112
Size: 1142 35 935
Fragments arranged by size:
1142 935 35
DraI TTT'AAA
Cuts at: 0 1953 2112
Size: 1953 159
EaeI y'GGCC_r
Cuts at: 0 869 1890 2112
Size: 869 1021 222
Fragments arranged by size:
1021 869 222
EarI CTCTTCn'nnn_
Cuts at: 0 75 104 671 1766 2112
Size: 75 29 567 1095 346
Fragments arranged by size:
1095 567 346 75 29
EcoICRI GAG'CTC
Cuts at: 0 189 2112
Size: 189 1923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 1190 1345 1823 2112
Size: 85 1105 155 478 289
Fragments arranged by size:
1105 478 289 155 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 619 2112
Size: 619 1493
EcoRV GAT'ATC
Cuts at: 0 1906 2112
Size: 1906 206
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1164 2112
Size: 1164 948
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 81 113 1215 1247 1567 1599 1956 1988
Size: 81 32 1102 32 320 32 357 32
Cuts at: 1988 2112
Size: 124
Fragments arranged by size:
1102 357 320 124 81 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1707 2112
Size: 1707 405
MfeI C'AATT_G
Cuts at: 0 1154 2112
Size: 1154 958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 1962 2112
Size: 204 1758 150
Fragments arranged by size:
1758 204 150
MscI TGG'CCA
Cuts at: 0 871 1892 2112
Size: 871 1021 220
Fragments arranged by size:
1021 871 220
MslI CAynn'nnrTG
Cuts at: 0 1849 1933 2038 2112
Size: 1849 84 105 74
Fragments arranged by size:
1849 105 84 74
MspA1I CmG'CkG
Cuts at: 0 1946 2112
Size: 1946 166
NcoI C'CATG_G
Cuts at: 0 1928 2112
Size: 1928 184
NsiI A_TGCA'T
Cuts at: 0 1511 2112
Size: 1511 601
NspI r_CATG'y
Cuts at: 0 334 898 2043 2107 2112
Size: 334 564 1145 64 5
Fragments arranged by size:
1145 564 334 64 5
PflMI CCAn_nnn'nTGG
Cuts at: 0 1981 2112
Size: 1981 131
PfoI T'CCnGG_A
Cuts at: 0 52 2112
Size: 52 2060
PmlI CAC'GTG
Cuts at: 0 1503 2112
Size: 1503 609
PsiI TTA'TAA
Cuts at: 0 1646 2112
Size: 1646 466
PstI C_TGCA'G
Cuts at: 0 269 2112
Size: 269 1843
PvuII CAG'CTG
Cuts at: 0 1946 2112
Size: 1946 166
SacI G_AGCT'C
Cuts at: 0 191 2112
Size: 191 1921
SapI GCTCTTCn'nnn_
Cuts at: 0 671 2112
Size: 671 1441
SfcI C'TryA_G
Cuts at: 0 265 1280 2112
Size: 265 1015 832
Fragments arranged by size:
1015 832 265
SmlI C'TyrA_G
Cuts at: 0 1111 1138 2112
Size: 1111 27 974
Fragments arranged by size:
1111 974 27
SphI G_CATG'C
Cuts at: 0 2107 2112
Size: 2107 5
SspI AAT'ATT
Cuts at: 0 1820 1897 2112
Size: 1820 77 215
Fragments arranged by size:
1820 215 77
StuI AGG'CCT
Cuts at: 0 2099 2112
Size: 2099 13
StyI C'CwwG_G
Cuts at: 0 647 863 983 1321 1928 2112
Size: 647 216 120 338 607 184
Fragments arranged by size:
647 607 338 216 184 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 976 1345 1593 2112
Size: 976 369 248 519
Fragments arranged by size:
976 519 369 248
TatI w'GTAC_w
Cuts at: 0 364 549 2112
Size: 364 185 1563
Fragments arranged by size:
1563 364 185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 71 103 728 760 1233 1265 1541 1573
Size: 71 32 625 32 473 32 276 32
Cuts at: 1573 2112
Size: 539
Fragments arranged by size:
625 539 473 276 71 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 904 2112
Size: 904 1208
XbaI T'CTAG_A
Cuts at: 0 954 2112
Size: 954 1158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 786 1776 2112
Size: 492 294 990 336
Fragments arranged by size:
990 492 336 294
ZraI GAC'GTC
Cuts at: 0 1614 2112
Size: 1614 498
Enzymes that do cut:
AatII AcuI AlfI AlwNI ApaLI ApoI BanI BanII
BbsI BciVI BclI BfrBI Bme1580I BpmI BpuEI BsaI
BsaAI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI EaeI EarI EcoICRI
Eco57MI EcoNI EcoRV FalI Hin4I HincII MfeI MmeI
MscI MslI MspA1I NcoI NsiI NspI PflMI PfoI
PmlI PsiI PstI PvuII SacI SapI SfcI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BglI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaBI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I ClaI DraIII DrdI EagI
EciI EcoO109I EcoRI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI XhoI XmaI
XmnI