Restriction map for pF1KA1162
 (Linear) (Six-base) MAPSORT of: KIAA1162.seq  Check: 6421  from: 1  to: 1047



KIAA1162.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     971    1047 
   Size:        235     736      76

  Fragments arranged by size: 

                 736     235      76
 
AleI CACnn'nnGTG

Cuts at:      0     991    1047 
   Size:        991      56
 
ApaI G_GGCC'C

Cuts at:      0     902    1047 
   Size:        902     145
 
ApoI r'AATT_y

Cuts at:      0     172     545    1047 
   Size:        172     373     502

  Fragments arranged by size: 

                 502     373     172
 
AvaI C'yCGr_G

Cuts at:      0      79     923    1047 
   Size:         79     844     124

  Fragments arranged by size: 

                 844     124      79
 
BanII G_rGCy'C

Cuts at:      0     902    1047 
   Size:        902     145
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     392    1007    1047 
   Size:        392     615      40

  Fragments arranged by size: 

                 615     392      40
 
BclI T'GATC_A

Cuts at:      0     891    1047 
   Size:        891     156
 
BglII A'GATC_T

Cuts at:      0     804    1047 
   Size:        804     243
 
Bme1580I G_kGCm'C

Cuts at:      0     902    1047 
   Size:        902     145
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1029    1047 
   Size:       1029      18
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     145     175    1047 
   Size:        145      30     872

  Fragments arranged by size: 

                 872     145      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      97     712     748     853     856     886     958    1047 
   Size:         97     615      36     105       3      30      72      89

  Fragments arranged by size: 

                 615     105      97      89      72      36      30       3
            
 
BseYI C'CCAG_C

Cuts at:      0     324     978    1047 
   Size:        324     654      69

  Fragments arranged by size: 

                 654     324      69
 
Bsp1286I G_dGCh'C

Cuts at:      0     902    1047 
   Size:        902     145
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     398    1047 
   Size:        398     649
 
BstBI TT'CG_AA

Cuts at:      0     383    1047 
   Size:        383     664
 
BstYI r'GATC_y

Cuts at:      0     804    1047 
   Size:        804     243
 
Bsu36I CC'TnA_GG

Cuts at:      0     941    1047 
   Size:        941     106
 
BtgI C'CryG_G

Cuts at:      0     184    1047 
   Size:        184     863
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     827    1047 
   Size:        827     220
 
EaeI y'GGCC_r

Cuts at:      0      41     300    1047 
   Size:         41     259     747

  Fragments arranged by size: 

                 747     259      41
 
EarI CTCTTCn'nnn_

Cuts at:      0     788     830    1047 
   Size:        788      42     217

  Fragments arranged by size: 

                 788     217      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     346    1047 
   Size:        346     701
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     971    1047 
   Size:        235     736      76

  Fragments arranged by size: 

                 736     235      76
 
EcoO109I rG'GnC_Cy

Cuts at:      0      76     898     899    1047 
   Size:         76     822       1     148

  Fragments arranged by size: 

                 822     148      76       1
 
EcoRI G'AATT_C

Cuts at:      0     545    1047 
   Size:        545     502
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     793     823    1047 
   Size:        793      30     224

  Fragments arranged by size: 

                 793     224      30
 
HaeII r_GCGC'y

Cuts at:      0      15      37    1047 
   Size:         15      22    1010

  Fragments arranged by size: 

                1010      22      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     498     530    1047 
   Size:        498      32     517

  Fragments arranged by size: 

                 517     498      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     158    1047 
   Size:        158     889
 
MslI CAynn'nnrTG

Cuts at:      0     991    1047 
   Size:        991      56
 
MspA1I CmG'CkG

Cuts at:      0     982    1047 
   Size:        982      65
 
NcoI C'CATG_G

Cuts at:      0     184    1047 
   Size:        184     863
 
NspI r_CATG'y

Cuts at:      0    1033    1047 
   Size:       1033      14
 
PspOMI G'GGCC_C

Cuts at:      0     898    1047 
   Size:        898     149
 
PstI C_TGCA'G

Cuts at:      0     395    1047 
   Size:        395     652
 
PvuII CAG'CTG

Cuts at:      0     982    1047 
   Size:        982      65
 
SexAI A'CCwGG_T

Cuts at:      0     288    1047 
   Size:        288     759
 
SfcI C'TryA_G

Cuts at:      0     391    1047 
   Size:        391     656
 
SmaI CCC'GGG

Cuts at:      0     925    1047 
   Size:        925     122
 
SmlI C'TyrA_G

Cuts at:      0    1008    1047 
   Size:       1008      39
 
SphI G_CATG'C

Cuts at:      0    1033    1047 
   Size:       1033      14
 
StyI C'CwwG_G

Cuts at:      0     184     587     655    1047 
   Size:        184     403      68     392

  Fragments arranged by size: 

                 403     392     184      68
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     145     177    1047 
   Size:        145      32     870

  Fragments arranged by size: 

                 870     145      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     141    1047 
   Size:        141     906
 
XmaI C'CCGG_G

Cuts at:      0     923    1047 
   Size:        923     124
 
XmnI GAAnn'nnTTC

Cuts at:      0     248    1047 
   Size:        248     799
 

 Enzymes that do cut:

     AcuI     AleI     ApaI     ApoI     AvaI    BanII     BbsI     BclI
    BglII Bme1580I    BpuEI    BsaXI    BseRI    BseYI Bsp1286I    BspMI
    BstBI    BstYI   Bsu36I     BtgI    BtgZI     EaeI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I     MmeI     MslI
   MspA1I     NcoI     NspI   PspOMI     PstI    PvuII    SexAI     SfcI
     SmaI     SmlI     SphI     StyI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
  BstZ17I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TatI
  Tth111I     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com