Restriction map for pF1KA1139
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# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AhdI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       BbrPI       
BciVI       BclI        BfrI        BsaI        BsiWI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrGI       Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        Eam1105I    EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HpaI        
KpnI        KspI        MfeI        MluI        MunI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XhoI        


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# ONE CUT ENZYMES (   46 enzymes) #
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AcsI          469  GAATTT               [GA]^AATT[CT]
AgeI           49  ACCGGT               A^CCGGT
Alw44I       1703  GTGCAC               G^TGCAC
Aor51HI       861  AGCGCT               AGC^GCT
ApaLI        1703  GTGCAC               G^TGCAC
ApoI          469  GAATTT               [GA]^AATT[CT]
Asp700I       783  GAATCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        2466  CCTAGG               C^CTAGG
BamHI         845  GGATCC               G^GATCC
BbeI         1907  GGCGCC               GGCGC^C
BbsI          444  GAAGAC               GAAGAC(2/6)
BglII        1421  AGATCT               A^GATCT
BlnI         2466  CCTAGG               C^CTAGG
BpuAI         444  GAAGAC               GAAGAC(2/6)
BsaAI         753  TACGTA               [CT]AC^GT[GA]
BsmBI        2634  CGTCTC               CGTCTC(1/5)
BsrDI         239  GCAATG               GCAATG(2/0)
BssHII        306  GCGCGC               G^CGCGC
BstAPI        822  GCAGCTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1596  GGTGACC              G^GT[ACGT]ACC
BstPI        1596  GGTGACC              G^GT[ACGT]ACC
DraIII       2717  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3450  GACCAGCTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         3489  GAAGAG               CTCTTC(1/4)
Eco47III      861  AGCGCT               AGC^GCT
EcoO65I      1596  GGTGACC              G^GT[ACGT]ACC
HindIII      2221  AAGCTT               A^AGCTT
KasI         1907  GGCGCC               G^GCGCC
Ksp632I      3489  GAAGAG               CTCTTC(1/4)
MseI         2969  TTAA                 T^TAA
NarI         1907  GGCGCC               GG^CGCC
NheI         3354  GCTAGC               G^CTAGC
NspI          245  GCATGC               [GA]CATG^[CT]
PinAI          49  ACCGGT               A^CCGGT
SapI         3489  GAAGAGC              GCTCTTC(1/4)
SexAI        1337  ACCAGGT              A^CC[AT]GGT
SfoI         1907  GGCGCC               GGC^GCC
SmaI         1014  CCCGGG               CCC^GGG
SnaBI         753  TACGTA               TAC^GTA
SphI          245  GCATGC               GCATG^C
StuI          836  AGGCCT               AGG^CCT
Tru9I        2969  TTAA                 T^TAA
Tsp509I       470  AATT                 ^AATT
XmaCI        1014  CCCGGG               C^CCGGG
XmaI         1014  CCCGGG               C^CCGGG
XmnI          783  GAATCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BcgI          888  CGATCCCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3560  GCACCATGTTCG        
BsaBI         636  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1935  GATGGCCATC          
BsiEI         951  CGGCCG               CG[GA][CT]^CG
             3257  CGGCCG              
BssSI         676  CACGAG               CACGAG(-5/-1)
              973  CACGAG              
BstXI        1281  CCATGGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1757  CCATGGGGCTGG        
EagI          951  CGGCCG               C^GGCCG
             3257  CGGCCG              
EclXI         951  CGGCCG               C^GGCCG
             3257  CGGCCG              
Eco52I        951  CGGCCG               C^GGCCG
             3257  CGGCCG              
MamI          636  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1935  GATGGCCATC          
SacI          187  GAGCTC               GAGCT^C
             1260  GAGCTC              


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccII       AccIII      AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApaI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaHI       BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspEI       BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MroI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XcmI        XhoII       
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