Restriction map for
pF1KA1035
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII AccII AcyI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspHI AspI AvrII BbeI
BglI BlnI BsaHI BsaI BseRI
BsiEI BsiHKAI BsiWI BsmBI BspDI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I FauI FseI
HaeII HgaI Hin1I HpaI KasI
KspI MfeI MluI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 3355 GGTACC G^GTACC
AccIII 2958 TCCGGA T^CCGGA
AclI 2796 AACGTT AA^CGTT
AflII 258 CTTAAG C^TTAAG
AflIII 3255 ACGTGT A^C[GA][CT]GT
AocI 584 CCTTAGG CC^T[ACGT]AGG
AseI 463 ATTAAT AT^TAAT
AsnI 463 ATTAAT AT^TAAT
Asp718I 3355 GGTACC G^GTACC
AviII 2162 TGCGCA TGC^GCA
BalI 3051 TGGCCA TGG^CCA
BamHI 933 GGATCC G^GATCC
BanII 470 GGGCTC G[GA]GC[CT]^C
BbrPI 3254 CACGTG CAC^GTG
BbsI 1676 GTCTTC GAAGAC(2/6)
BbvCI 2602 CCTCAGC CCTCAGC(-5/-2)
BcgI 510 GCAGAATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1234 CCGGG CC^[GC]GG
BfrI 258 CTTAAG C^TTAAG
BmyI 470 GGGCTC G[AGT]GC[ACT]^C
BpuAI 1676 GTCTTC GAAGAC(2/6)
BsaBI 2962 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2958 TCCGGA [AT]^CCGG[AT]
BseAI 2958 TCCGGA T^CCGGA
BsmAI 3100 GAGAC GTCTC(1/5)
Bsp1286I 470 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 2007 TGTACA T^GTACA
BspEI 2958 TCCGGA T^CCGGA
BspHI 2810 TCATGA T^CATGA
BspMI 2452 GCAGGT ACCTGC(4/8)
BsrFI 782 ACCGGC [GA]^CCGG[CT]
BsrGI 2007 TGTACA T^GTACA
Bsu36I 584 CCTTAGG CC^T[ACGT]AGG
CfoI 2163 GCGC GCG^C
Cfr10I 782 ACCGGC [GA]^CCGG[CT]
EaeI 3051 TGGCCA [CT]^GGCC[GA]
Eco81I 584 CCTTAGG CC^T[ACGT]AGG
EcoNI 1375 CCTACGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2059 GATATC GAT^ATC
EcoT22I 2569 ATGCAT ATGCA^T
FspI 2162 TGCGCA TGC^GCA
HhaI 2163 GCGC GCG^C
HinP1I 2163 GCGC G^CGC
HincII 213 GTCAAC GT[CT]^[GA]AC
HindII 213 GTCAAC GT[CT]^[GA]AC
HindIII 1771 AAGCTT A^AGCTT
KpnI 3355 GGTACC GGTAC^C
MamI 2962 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3051 TGGCCA TGG^CCA
MroI 2958 TCCGGA T^CCGGA
MscI 3051 TGGCCA TGG^CCA
NciI 1234 CCGGG CC^[GC]GG
NsiI 2569 ATGCAT ATGCA^T
PflMI 2378 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3254 CACGTG CAC^GTG
PmlI 3254 CACGTG CAC^GTG
PshAI 1669 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 463 ATTAAT AT^TAAT
Psp1406I 2796 AACGTT AA^CGTT
RcaI 2810 TCATGA T^CATGA
SexAI 2271 ACCAGGT A^CC[AT]GGT
SnaBI 2550 TACGTA TAC^GTA
SspBI 2007 TGTACA T^GTACA
Van91I 2378 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3509 CCACTTATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 721 GTAGAC GT^[AC][GT]AC
1473 GTCTAC
AciI 771 CCGC CCGC(-3/-1)
1966 CCGC
AlwNI 104 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
2444 CAGTTGCTG
Asp700I 969 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1553 GAACTATTTC
AvaI 2750 CTCGGG C^[CT]CG[GA]G
3359 CCCGAG
BanI 767 GGCACC G^G[CT][GA]CC
3355 GGTACC
BclI 1542 TGATCA T^GATCA
3324 TGATCA
BglII 234 AGATCT A^GATCT
1251 AGATCT
BlpI 88 GCTGAGC GC^T[ACGT]AGC
1060 GCTCAGC
Bpu1102I 88 GCTGAGC GC^T[ACGT]AGC
1060 GCTCAGC
BsgI 1779 CTGCAC GTGCAG(16/14)
3563 GTGCAG
BsoBI 2750 CTCGGG C^[CT]CG[GA]G
3359 CCCGAG
BstAPI 501 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2368 GCATTAAATGC
CelII 88 GCTGAGC GC^T[ACGT]AGC
1060 GCTCAGC
FbaI 1542 TGATCA T^GATCA
3324 TGATCA
NspI 794 ACATGC [GA]CATG^[CT]
1708 GCATGT
ScaI 3307 AGTACT AGT^ACT
3639 AGTACT
StuI 1595 AGGCCT AGG^CCT
1996 AGGCCT
XmnI 969 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1553 GAACTATTTC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BciVI BfaI BpmI
BsaAI BsaJI BsiYI BslI BsmFI
BsmI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DsaI EarI EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII