Restriction map for
pF1KA1035
(Circular) (Six-base) MAPSORT of: pF1KA1035.seq Check: 3285 from: 1 to: 6791
pF1KA1035 6791 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2551 2551
Size: 6791
AccI GT'mk_AC
Cuts at: 830 1582 3829 830
Size: 752 2247 3792
Fragments arranged by size:
3792 2247 752
Acc65I G'GTAC_C
Cuts at: 3463 3807 3463
Size: 344 6447
AclI AA'CG_TT
Cuts at: 2905 6631 2905
Size: 3726 3065
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 631 1211 1925 4616 5048 5401 6063 188
Size: 443 580 714 2691 432 353 662 916
Fragments arranged by size:
2691 916 714 662 580 443 432 353
AflII C'TTAA_G
Cuts at: 366 366
Size: 6791
AflIII A'CryG_T
Cuts at: 3363 4143 5943 6224 3363
Size: 780 1800 281 3930
Fragments arranged by size:
3930 1800 780 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1367 1399 1850 1882 3632 3664 1367
Size: 32 451 32 1750 32 4494
Fragments arranged by size:
4494 1750 451 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 217 2557 4096 5534 217
Size: 2340 1539 1438 1474
Fragments arranged by size:
2340 1539 1474 1438
ApaLI G'TGCA_C
Cuts at: 5629 5629
Size: 6791
ApoI r'AATT_y
Cuts at: 239 311 550 597 612 681 753 920 995
Size: 72 239 47 15 69 72 167 75
Cuts at: 995 1284 1509 1929 2239 2251 3493 3795 6144
Size: 289 225 420 310 12 1242 302 2349
Cuts at: 6144 6400 239
Size: 256 630
Fragments arranged by size:
2349 1242 630 420 310 302 289 256
239 225 167 75 72 72 69 47
15 12
AseI AT'TA_AT
Cuts at: 20 572 20
Size: 552 6239
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6791
AvaI C'yCGr_G
Cuts at: 2858 3467 3811 2858
Size: 609 344 5838
Fragments arranged by size:
5838 609 344
BamHI G'GATC_C
Cuts at: 1041 3816 1041
Size: 2775 4016
BanI G'GyrC_C
Cuts at: 875 3463 3807 4221 4469 4504 875
Size: 2588 344 414 248 35 3162
Fragments arranged by size:
3162 2588 414 344 248 35
BanII G_rGCy'C
Cuts at: 582 3805 4835 582
Size: 3223 1030 2538
Fragments arranged by size:
3223 2538 1030
BbeI G_GCGC'C
Cuts at: 4225 4473 4225
Size: 248 6543
BbsI GAAGACnn'nnnn_
Cuts at: 1777 1777
Size: 6791
BbvCI CC'TCA_GC
Cuts at: 2711 2711
Size: 6791
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 607 641 607
Size: 34 6757
BciVI GTATCCnnnnn_n'
Cuts at: 699 3252 3259 4689 5745 6791 699
Size: 2553 7 1430 1056 1046 699
Fragments arranged by size:
2553 1430 1056 1046 699 7
BclI T'GATC_A
Cuts at: 1650 3432 4311 1650
Size: 1782 879 4130
Fragments arranged by size:
4130 1782 879
BfrBI ATG'CAT
Cuts at: 2679 2679
Size: 6791
BglII A'GATC_T
Cuts at: 342 1359 4306 6343 342
Size: 1017 2947 2037 790
Fragments arranged by size:
2947 2037 1017 790
BlpI GC'TnA_GC
Cuts at: 197 1169 3903 197
Size: 972 2734 3085
Fragments arranged by size:
3085 2734 972
Bme1580I G_kGCm'C
Cuts at: 4416 4509 5633 4416
Size: 93 1124 5574
Fragments arranged by size:
5574 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 4003 4084 4419 6563 4003
Size: 81 335 2144 4231
Fragments arranged by size:
4231 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5158 5158
Size: 6791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 1044 1913 2891 202
Size: 842 869 978 4102
Fragments arranged by size:
4102 978 869 842
Bpu10I CC'TnA_GC
Cuts at: 1388 2711 3095 1388
Size: 1323 384 5084
Fragments arranged by size:
5084 1323 384
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2120 2526 3722 3967 4846 5560 5858 2120
Size: 406 1196 245 879 714 298 3053
Fragments arranged by size:
3053 1196 879 714 406 298 245
BsaI GGTCTCn'nnnn_
Cuts at: 6495 6495
Size: 6791
BsaAI yAC'GTr
Cuts at: 1465 2660 3364 4144 1465
Size: 1195 704 780 4112
Fragments arranged by size:
4112 1195 780 704
BsaBI GATnn'nnATC
Cuts at: 6 3074 4305 6342 6
Size: 3068 1231 2037 455
Fragments arranged by size:
3068 2037 1231 455
BsaHI Gr'CG_yC
Cuts at: 4222 4470 5172 6668 4222
Size: 248 702 1496 4345
Fragments arranged by size:
4345 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3066 3980 4501 5179 5590 5737 6011 3066
Size: 914 521 678 411 147 274 3846
Fragments arranged by size:
3846 914 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1088 1118 1961 1991 1088
Size: 30 843 30 5888
Fragments arranged by size:
5888 843 30 30
BseYI C'CCAG_C
Cuts at: 1331 3303 4216 4974 5639 6047 1331
Size: 1972 913 758 665 408 2075
Fragments arranged by size:
2075 1972 913 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1872 3692 1872
Size: 1820 4971
BsiEI CG_ry'CG
Cuts at: 104 4333 4379 5609 104
Size: 4229 46 1230 1286
Fragments arranged by size:
4229 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3805 4583 4773 5633 3805
Size: 778 190 860 4963
Fragments arranged by size:
4963 860 778 190
BsmI GAATG_Cn'
Cuts at: 371 471 2378 371
Size: 100 1907 4784
Fragments arranged by size:
4784 1907 100
Bsp1286I G_dGCh'C
Cuts at: 582 3805 4416 4509 4583 4773 4835 5633 582
Size: 3223 611 93 74 190 62 798 1740
Fragments arranged by size:
3223 1740 798 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3066 3066
Size: 6791
BspHI T'CATG_A
Cuts at: 2918 5223 2918
Size: 2305 4486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2551 3841 4055 4357 4738 2551
Size: 1290 214 302 381 4604
Fragments arranged by size:
4604 1290 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5083 5137 6621 6786 50
Size: 5033 54 1484 165 55
Fragments arranged by size:
5033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 793 1720 2795 3274 4703 793
Size: 927 1075 479 1429 2881
Fragments arranged by size:
2881 1429 1075 927 479
BsrFI r'CCGG_y
Cuts at: 890 4789 4970 890
Size: 3899 181 2711
Fragments arranged by size:
3899 2711 181
BsrGI T'GTAC_A
Cuts at: 2115 2115
Size: 6791
BssSI C'ACGA_G
Cuts at: 5062 5770 5062
Size: 708 6083
BstAPI GCAn_nnn'nTGC
Cuts at: 615 2482 4168 615
Size: 1867 1686 3238
Fragments arranged by size:
3238 1867 1686
BstBI TT'CG_AA
Cuts at: 5152 5152
Size: 6791
BstYI r'GATC_y
Cuts at: 1 342 1041 1359 1593 3816 4283 4306 4641
Size: 341 699 318 234 2223 467 23 335
Cuts at: 4641 4887 5193 5205 5291 6343 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2223 1052 699 467 449 341 335 318
306 246 234 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 693 693
Size: 6791
BtgI C'CryG_G
Cuts at: 1677 2491 3407 1677
Size: 814 916 5061
Fragments arranged by size:
5061 916 814
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 610 2998 4741 4922 5085 610
Size: 2388 1743 181 163 2316
Fragments arranged by size:
2388 2316 1743 181 163
BtsI GCAGTG_nn'
Cuts at: 215 2933 3055 3128 4034 215
Size: 2718 122 73 906 2972
Fragments arranged by size:
2972 2718 906 122 73
DraI TTT'AAA
Cuts at: 726 935 2967 3790 726
Size: 209 2032 823 3727
Fragments arranged by size:
3727 2032 823 209
DrdI GACnn_nn'nnGTC
Cuts at: 4497 5841 4497
Size: 1344 5447
EaeI y'GGCC_r
Cuts at: 3159 4376 4550 4941 4968 6313 3159
Size: 1217 174 391 27 1345 3637
Fragments arranged by size:
3637 1345 1217 391 174 27
EagI C'GGCC_G
Cuts at: 4376 4376
Size: 6791
EarI CTCTTCn'nnn_
Cuts at: 402 2216 3473 4814 5024 402
Size: 1814 1257 1341 210 2169
Fragments arranged by size:
2169 1814 1341 1257 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2063 4999 5741 5887 6601 2063
Size: 2936 742 146 714 2253
Fragments arranged by size:
2936 2253 742 714 146
EcoICRI GAG'CTC
Cuts at: 3803 3803
Size: 6791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 202 631 1044 1211 1913 1925 2891 4616
Size: 14 429 413 167 702 12 966 1725
Cuts at: 4616 5048 5401 6063 188
Size: 432 353 662 916
Fragments arranged by size:
1725 966 916 702 662 432 429 413
353 167 14 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1487 1487
Size: 6791
EcoO109I rG'GnC_Cy
Cuts at: 3930 3930
Size: 6791
EcoRI G'AATT_C
Cuts at: 681 753 1509 3795 681
Size: 72 756 2286 3677
Fragments arranged by size:
3677 2286 756 72
EcoRV GAT'ATC
Cuts at: 2169 2169
Size: 6791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1602 3766 4810 1602
Size: 2164 1044 3583
Fragments arranged by size:
3583 2164 1044
FspI TGC'GCA
Cuts at: 2272 4572 2272
Size: 2300 4491
HaeII r_GCGC'y
Cuts at: 4225 4473 5703 6474 4225
Size: 248 1230 771 4542
Fragments arranged by size:
4542 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 827 859 1026 1058 1088 1120 1408
Size: 32 422 32 167 32 30 32 288
Cuts at: 1408 1440 1486 1518 1534 1566 1909 1941 1961
Size: 32 46 32 16 32 343 32 20
Cuts at: 1961 1993 4633 4663 4665 4695 373
Size: 32 2640 30 2 30 2469
Fragments arranged by size:
2640 2469 422 343 288 167 46 32
32 32 32 32 32 32 32 32
30 30 30 20 16 2
HincII GTy'rAC
Cuts at: 323 3830 323
Size: 3507 3284
HindIII A'AGCT_T
Cuts at: 1879 1879
Size: 6791
KasI G'GCGC_C
Cuts at: 4221 4469 4221
Size: 248 6543
KpnI G_GTAC'C
Cuts at: 3467 3811 3467
Size: 344 6447
MluI A'CGCG_T
Cuts at: 6224 6224
Size: 6791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2447 2888 5550 5734 2447
Size: 441 2662 184 3504
Fragments arranged by size:
3504 2662 441 184
MscI TGG'CCA
Cuts at: 3161 4552 3161
Size: 1391 5400
MslI CAynn'nnrTG
Cuts at: 881 2162 2621 3224 4780 4907 881
Size: 1281 459 603 1556 127 2765
Fragments arranged by size:
2765 1556 1281 603 459 127
MspA1I CmG'CkG
Cuts at: 394 2765 3323 3902 4216 4576 5360 5605 394
Size: 2371 558 579 314 360 784 245 1580
Fragments arranged by size:
2371 1580 784 579 558 360 314 245
NaeI GCC'GGC
Cuts at: 4972 4972
Size: 6791
NarI GG'CG_CC
Cuts at: 4222 4470 4222
Size: 248 6543
NcoI C'CATG_G
Cuts at: 1677 2491 3407 1677
Size: 814 916 5061
Fragments arranged by size:
5061 916 814
NgoMIV G'CCGG_C
Cuts at: 4970 4970
Size: 6791
NsiI A_TGCA'T
Cuts at: 2681 2681
Size: 6791
NspI r_CATG'y
Cuts at: 906 1820 3844 4344 5947 906
Size: 914 2024 500 1603 1750
Fragments arranged by size:
2024 1750 1603 914 500
PciI A'CATG_T
Cuts at: 5943 5943
Size: 6791
PflMI CCAn_nnn'nTGG
Cuts at: 2492 2492
Size: 6791
PfoI T'CCnGG_A
Cuts at: 2868 2868
Size: 6791
PmeI GTTT'AAAC
Cuts at: 3790 3790
Size: 6791
PmlI CAC'GTG
Cuts at: 3364 3364
Size: 6791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5237 5269 5237
Size: 32 6759
PshAI GACnn'nnGTC
Cuts at: 1781 1781
Size: 6791
PsiI TTA'TAA
Cuts at: 766 766
Size: 6791
PstI C_TGCA'G
Cuts at: 1764 1783 2562 3123 3838 4523 1764
Size: 19 779 561 715 685 4032
Fragments arranged by size:
4032 779 715 685 561 19
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6791
PvuII CAG'CTG
Cuts at: 394 2765 3323 4216 4576 394
Size: 2371 558 893 360 2609
Fragments arranged by size:
2609 2371 893 558 360
RsrII CG'GwC_CG
Cuts at: 4986 4986
Size: 6791
SacI G_AGCT'C
Cuts at: 3805 3805
Size: 6791
SalI G'TCGA_C
Cuts at: 3828 3828
Size: 6791
SapI GCTCTTCn'nnn_
Cuts at: 4814 5024 4814
Size: 210 6581
SbfI CC_TGCA'GG
Cuts at: 3838 3838
Size: 6791
ScaI AGT'ACT
Cuts at: 3417 3749 6308 3417
Size: 332 2559 3900
Fragments arranged by size:
3900 2559 332
SexAI A'CCwGG_T
Cuts at: 2379 2379
Size: 6791
SfcI C'TryA_G
Cuts at: 33 299 827 1176 1760 1779 2558 3119 3324
Size: 266 528 349 584 19 779 561 205
Cuts at: 3324 3834 4519 5487 5678 33
Size: 510 685 968 191 1146
Fragments arranged by size:
1146 968 779 685 584 561 528 510
349 266 205 191 19
SfoI GGC'GCC
Cuts at: 4223 4471 4223
Size: 248 6543
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6791
SmaI CCC'GGG
Cuts at: 3813 3813
Size: 6791
SmlI C'TyrA_G
Cuts at: 366 2135 2541 3737 3946 4861 5575 5837 366
Size: 1769 406 1196 209 915 714 262 1320
Fragments arranged by size:
1769 1320 1196 915 714 406 262 209
SnaBI TAC'GTA
Cuts at: 2660 2660
Size: 6791
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6791
SphI G_CATG'C
Cuts at: 3844 4344 3844
Size: 500 6291
SspI AAT'ATT
Cuts at: 446 1731 2035 446
Size: 1285 304 5202
Fragments arranged by size:
5202 1285 304
StuI AGG'CCT
Cuts at: 1705 2106 1705
Size: 401 6390
StyI C'CwwG_G
Cuts at: 1677 1706 2491 2771 3137 3407 3925 4279 1677
Size: 29 785 280 366 270 518 354 4189
Fragments arranged by size:
4189 785 518 366 354 280 270 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1727 4374 4507 4966 5175 1727
Size: 2647 133 459 209 3343
Fragments arranged by size:
3343 2647 459 209 133
TatI w'GTAC_w
Cuts at: 2115 3390 3415 3747 6306 2115
Size: 1275 25 332 2559 2600
Fragments arranged by size:
2600 2559 1275 332 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6308 6340 6308
Size: 32 6759
Tth111I GACn'n_nGTC
Cuts at: 4588 4588
Size: 6791
XbaI T'CTAG_A
Cuts at: 3822 3822
Size: 6791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3624 4286 3624
Size: 662 6129
XmaI C'CCGG_G
Cuts at: 3811 3811
Size: 6791
XmnI GAAnn'nnTTC
Cuts at: 1081 1665 1081
Size: 584 6207
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BglI BmgBI BmtI BseRI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PpuMI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SwaI XhoI ZraI