Restriction map for
pF1KA1035
(Linear) (Six-base) MAPSORT of: KIAA1035.seq Check: 503 from: 1 to: 3678
KIAA1035.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2443 3678
Size: 2443 1235
AccI GT'mk_AC
Cuts at: 0 722 1474 3678
Size: 722 752 2204
Fragments arranged by size:
2204 752 722
Acc65I G'GTAC_C
Cuts at: 0 3355 3678
Size: 3355 323
AclI AA'CG_TT
Cuts at: 0 2797 3678
Size: 2797 881
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 523 1103 1817 3678
Size: 80 443 580 714 1861
Fragments arranged by size:
1861 714 580 443 80
AflII C'TTAA_G
Cuts at: 0 258 3678
Size: 258 3420
AflIII A'CryG_T
Cuts at: 0 3255 3678
Size: 3255 423
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1259 1291 1742 1774 3524 3556 3678
Size: 1259 32 451 32 1750 32 122
Fragments arranged by size:
1750 1259 451 122 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 109 2449 3678
Size: 109 2340 1229
Fragments arranged by size:
2340 1229 109
ApoI r'AATT_y
Cuts at: 0 131 203 442 489 504 573 645 812
Size: 131 72 239 47 15 69 72 167
Cuts at: 812 887 1176 1401 1821 2131 2143 3385 3678
Size: 75 289 225 420 310 12 1242 293
Fragments arranged by size:
1242 420 310 293 289 239 225 167
131 75 72 72 69 47 15 12
AseI AT'TA_AT
Cuts at: 0 464 3678
Size: 464 3214
AvaI C'yCGr_G
Cuts at: 0 2750 3359 3678
Size: 2750 609 319
Fragments arranged by size:
2750 609 319
BamHI G'GATC_C
Cuts at: 0 933 3678
Size: 933 2745
BanI G'GyrC_C
Cuts at: 0 767 3355 3678
Size: 767 2588 323
Fragments arranged by size:
2588 767 323
BanII G_rGCy'C
Cuts at: 0 474 3678
Size: 474 3204
BbsI GAAGACnn'nnnn_
Cuts at: 0 1669 3678
Size: 1669 2009
BbvCI CC'TCA_GC
Cuts at: 0 2603 3678
Size: 2603 1075
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 499 533 3678
Size: 499 34 3145
Fragments arranged by size:
3145 499 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 591 3144 3151 3678
Size: 591 2553 7 527
Fragments arranged by size:
2553 591 527 7
BclI T'GATC_A
Cuts at: 0 1542 3324 3678
Size: 1542 1782 354
Fragments arranged by size:
1782 1542 354
BfrBI ATG'CAT
Cuts at: 0 2571 3678
Size: 2571 1107
BglII A'GATC_T
Cuts at: 0 234 1251 3678
Size: 234 1017 2427
Fragments arranged by size:
2427 1017 234
BlpI GC'TnA_GC
Cuts at: 0 89 1061 3678
Size: 89 972 2617
Fragments arranged by size:
2617 972 89
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 936 1805 2783 3678
Size: 94 842 869 978 895
Fragments arranged by size:
978 895 869 842 94
Bpu10I CC'TnA_GC
Cuts at: 0 1280 2603 2987 3678
Size: 1280 1323 384 691
Fragments arranged by size:
1323 1280 691 384
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2012 2418 3614 3678
Size: 2012 406 1196 64
Fragments arranged by size:
2012 1196 406 64
BsaAI yAC'GTr
Cuts at: 0 1357 2552 3256 3678
Size: 1357 1195 704 422
Fragments arranged by size:
1357 1195 704 422
BsaBI GATnn'nnATC
Cuts at: 0 2966 3678
Size: 2966 712
BsaWI w'CCGG_w
Cuts at: 0 2958 3678
Size: 2958 720
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 980 1010 1853 1883 3678
Size: 980 30 843 30 1795
Fragments arranged by size:
1795 980 843 30 30
BseYI C'CCAG_C
Cuts at: 0 1223 3195 3678
Size: 1223 1972 483
Fragments arranged by size:
1972 1223 483
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1764 3584 3678
Size: 1764 1820 94
Fragments arranged by size:
1820 1764 94
BsmI GAATG_Cn'
Cuts at: 0 263 363 2270 3678
Size: 263 100 1907 1408
Fragments arranged by size:
1907 1408 263 100
Bsp1286I G_dGCh'C
Cuts at: 0 474 3678
Size: 474 3204
BspEI T'CCGG_A
Cuts at: 0 2958 3678
Size: 2958 720
BspHI T'CATG_A
Cuts at: 0 2810 3678
Size: 2810 868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2443 3678
Size: 2443 1235
BsrDI GCAATG_nn'
Cuts at: 0 685 1612 2687 3166 3678
Size: 685 927 1075 479 512
Fragments arranged by size:
1075 927 685 512 479
BsrFI r'CCGG_y
Cuts at: 0 782 3678
Size: 782 2896
BsrGI T'GTAC_A
Cuts at: 0 2007 3678
Size: 2007 1671
BstAPI GCAn_nnn'nTGC
Cuts at: 0 507 2374 3678
Size: 507 1867 1304
Fragments arranged by size:
1867 1304 507
BstYI r'GATC_y
Cuts at: 0 234 933 1251 1485 3678
Size: 234 699 318 234 2193
Fragments arranged by size:
2193 699 318 234 234
Bsu36I CC'TnA_GG
Cuts at: 0 585 3678
Size: 585 3093
BtgI C'CryG_G
Cuts at: 0 1569 2383 3299 3678
Size: 1569 814 916 379
Fragments arranged by size:
1569 916 814 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 502 2890 3678
Size: 502 2388 788
Fragments arranged by size:
2388 788 502
BtsI GCAGTG_nn'
Cuts at: 0 107 2825 2947 3020 3678
Size: 107 2718 122 73 658
Fragments arranged by size:
2718 658 122 107 73
DraI TTT'AAA
Cuts at: 0 618 827 2859 3678
Size: 618 209 2032 819
Fragments arranged by size:
2032 819 618 209
EaeI y'GGCC_r
Cuts at: 0 3051 3678
Size: 3051 627
EarI CTCTTCn'nnn_
Cuts at: 0 294 2108 3365 3678
Size: 294 1814 1257 313
Fragments arranged by size:
1814 1257 313 294
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1955 3678
Size: 1955 1723
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 94 523 936 1103 1805 1817 2783
Size: 80 14 429 413 167 702 12 966
Cuts at: 2783 3678
Size: 895
Fragments arranged by size:
966 895 702 429 413 167 80 14
12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1379 3678
Size: 1379 2299
EcoRI G'AATT_C
Cuts at: 0 573 645 1401 3678
Size: 573 72 756 2277
Fragments arranged by size:
2277 756 573 72
EcoRV GAT'ATC
Cuts at: 0 2061 3678
Size: 2061 1617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1494 3658 3678
Size: 1494 2164 20
Fragments arranged by size:
2164 1494 20
FspI TGC'GCA
Cuts at: 0 2164 3678
Size: 2164 1514
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 719 751 918 950 980 1012
Size: 265 32 422 32 167 32 30 32
Cuts at: 1012 1300 1332 1378 1410 1426 1458 1801 1833
Size: 288 32 46 32 16 32 343 32
Cuts at: 1833 1853 1885 3678
Size: 20 32 1793
Fragments arranged by size:
1793 422 343 288 265 167 46 32
32 32 32 32 32 32 32 32
30 20 16
HincII GTy'rAC
Cuts at: 0 215 3678
Size: 215 3463
HindIII A'AGCT_T
Cuts at: 0 1771 3678
Size: 1771 1907
KpnI G_GTAC'C
Cuts at: 0 3359 3678
Size: 3359 319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2339 2780 3678
Size: 2339 441 898
Fragments arranged by size:
2339 898 441
MscI TGG'CCA
Cuts at: 0 3053 3678
Size: 3053 625
MslI CAynn'nnrTG
Cuts at: 0 773 2054 2513 3116 3678
Size: 773 1281 459 603 562
Fragments arranged by size:
1281 773 603 562 459
MspA1I CmG'CkG
Cuts at: 0 286 2657 3215 3678
Size: 286 2371 558 463
Fragments arranged by size:
2371 558 463 286
NcoI C'CATG_G
Cuts at: 0 1569 2383 3299 3678
Size: 1569 814 916 379
Fragments arranged by size:
1569 916 814 379
NsiI A_TGCA'T
Cuts at: 0 2573 3678
Size: 2573 1105
NspI r_CATG'y
Cuts at: 0 798 1712 3678
Size: 798 914 1966
Fragments arranged by size:
1966 914 798
PflMI CCAn_nnn'nTGG
Cuts at: 0 2384 3678
Size: 2384 1294
PfoI T'CCnGG_A
Cuts at: 0 2760 3678
Size: 2760 918
PmlI CAC'GTG
Cuts at: 0 3256 3678
Size: 3256 422
PshAI GACnn'nnGTC
Cuts at: 0 1673 3678
Size: 1673 2005
PsiI TTA'TAA
Cuts at: 0 658 3678
Size: 658 3020
PstI C_TGCA'G
Cuts at: 0 1656 1675 2454 3015 3678
Size: 1656 19 779 561 663
Fragments arranged by size:
1656 779 663 561 19
PvuII CAG'CTG
Cuts at: 0 286 2657 3215 3678
Size: 286 2371 558 463
Fragments arranged by size:
2371 558 463 286
ScaI AGT'ACT
Cuts at: 0 3309 3641 3678
Size: 3309 332 37
Fragments arranged by size:
3309 332 37
SexAI A'CCwGG_T
Cuts at: 0 2271 3678
Size: 2271 1407
SfcI C'TryA_G
Cuts at: 0 191 719 1068 1652 1671 2450 3011 3216
Size: 191 528 349 584 19 779 561 205
Cuts at: 3216 3678
Size: 462
Fragments arranged by size:
779 584 561 528 462 349 205 191
19
SmlI C'TyrA_G
Cuts at: 0 258 2027 2433 3629 3678
Size: 258 1769 406 1196 49
Fragments arranged by size:
1769 1196 406 258 49
SnaBI TAC'GTA
Cuts at: 0 2552 3678
Size: 2552 1126
SspI AAT'ATT
Cuts at: 0 338 1623 1927 3678
Size: 338 1285 304 1751
Fragments arranged by size:
1751 1285 338 304
StuI AGG'CCT
Cuts at: 0 1597 1998 3678
Size: 1597 401 1680
Fragments arranged by size:
1680 1597 401
StyI C'CwwG_G
Cuts at: 0 1569 1598 2383 2663 3029 3299 3678
Size: 1569 29 785 280 366 270 379
Fragments arranged by size:
1569 785 379 366 280 270 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1619 3678
Size: 1619 2059
TatI w'GTAC_w
Cuts at: 0 2007 3282 3307 3639 3678
Size: 2007 1275 25 332 39
Fragments arranged by size:
2007 1275 332 39 25
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3516 3678
Size: 3516 162
XmnI GAAnn'nnTTC
Cuts at: 0 973 1557 3678
Size: 973 584 2121
Fragments arranged by size:
2121 973 584
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AloI
AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseYI BsgI
BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI
BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraI EaeI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI
Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I
NcoI NsiI NspI PflMI PfoI PmlI PshAI PsiI
PstI PvuII ScaI SexAI SfcI SmlI SnaBI SspI
StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AlfI ApaI ApaLI
AscI AsiSI AvrII BaeI BbeI BglI Bme1580I BmgBI
BmrI BmtI BplI BsaI BsaHI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsrBI BssHII BssSI BstBI BstEII BstXI
BstZ17I ClaI CspCI DraIII DrdI EagI EcoICRI EcoO109I
FseI FspAI HaeII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PpiI PpuMI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SmaI SpeI SphI SrfI SwaI
TstI Tth111I XbaI XhoI XmaI ZraI