Restriction map for pF1KA1017
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BbeI        BbrPI       BbvCI       
BcgI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiWI       BsmBI       BsoBI       
BspDI       BspEI       BspHI       BsrBI       BsrFI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco52I      EcoNI       EcoO65I     
EcoT22I     FauI        FseI        FspI        HgaI        
Hin1I       HpaI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    RcaI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       Sse8387I    SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AciI         2373  GCGG                 CCGC(-3/-1)
AflIII       1935  ACATGT               A^C[GA][CT]GT
AocI         2961  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       731  AGCGCT               AGC^GCT
ApaI         2877  GGGCCC               GGGCC^C
BcnI         2216  CCCGG                CC^[GC]GG
BglII        2142  AGATCT               A^GATCT
BsiEI        3018  CGATCG               CG[GA][CT]^CG
BsmI         1232  GCATTC               GAATGC(1/-1)
Bsp1407I      195  TGTACA               T^GTACA
BspLU11I     1935  ACATGT               A^CATGT
BsrGI         195  TGTACA               T^GTACA
Bsu36I       2961  CCTGAGG              CC^T[ACGT]AGG
DraI         1099  TTTAAA               TTT^AAA
Eco47III      731  AGCGCT               AGC^GCT
Eco81I       2961  CCTGAGG              CC^T[ACGT]AGG
HaeII         731  AGCGCT               [GA]GCGC^[CT]
HapII        2217  CCGG                 C^CGG
HincII        181  GTTGAC               GT[CT]^[GA]AC
HindII        181  GTTGAC               GT[CT]^[GA]AC
HpaII        2217  CCGG                 C^CGG
MspI         2217  CCGG                 C^CGG
NciI         2216  CCCGG                CC^[GC]GG
PvuI         3018  CGATCG               CGAT^CG
SacI         1369  GAGCTC               GAGCT^C
SapI         2245  GCTCTTC              GCTCTTC(1/4)
SpeI         1459  ACTAGT               A^CTAGT
SphI         3002  GCATGC               GCATG^C
SspBI         195  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Asp700I       335  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1213  GAAACTTTTC          
BamHI         511  GGATCC               G^GATCC
             2720  GGATCC              
BanI          880  GGCACC               G^G[CT][GA]CC
             1330  GGCACC              
BciVI          51  GGATAC               GTATCC(6/5)
             2073  GGATAC              
BclI           81  TGATCA               T^GATCA
              498  TGATCA              
BsaI         1362  GAGACC               GGTCTC(1/5)
             2728  GAGACC              
BsiYI        1422  CCCAAAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2880  CCCAGATCGGG         
BslI         1422  CCCAAAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2880  CCCAGATCGGG         
BssSI        1341  CACGAG               CACGAG(-5/-1)
             2778  CACGAG              
BstAPI        136  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              558  GCATTGTGTGC         
BstXI        2465  CCAGCACAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2834  CCATCAATGTGG        
CfoI          732  GCGC                 GCG^C
             1838  GCGC                
EcoRI        1105  GAATTC               G^AATTC
             1908  GAATTC              
FbaI           81  TGATCA               T^GATCA
              498  TGATCA              
HhaI          732  GCGC                 GCG^C
             1838  GCGC                
HinP1I        732  GCGC                 G^CGC
             1838  GCGC                
HindIII      2041  AAGCTT               A^AGCTT
             2746  AAGCTT              
NcoI          876  CCATGG               C^CATGG
             2873  CCATGG              
PpuMI        1127  AGGACCT              [GA]G^G[AT]CC[CT]
             1532  AGGACCC             
ScaI         1958  AGTACT               AGT^ACT
             1997  AGTACT              
SfaNI        2449  GATGC                GCATC(5/9)
              992  GCATC               
SmlI         2386  CTTGAG               C^T[CT][GA]AG
             2923  CTTGAG              
SspI         1704  AATATT               AAT^ATT
             2671  AATATT              
TaqI          243  TCGA                 T^CGA
             2250  TCGA                
TthHB8I       243  TCGA                 T^CGA
             2250  TCGA                
XcmI         1928  CCAGTGGACATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2881  CCAGATCGGGCTTGG     
XmnI          335  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1213  GAAACTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        AlwNI       
ApoI        AspHI       AvaII       BanII       BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiHKAI     BsmAI       BsmFI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
EcoO109I    EcoRII      EcoRV       EcoT14I     Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        StuI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XhoII       
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