Restriction map for
pF1KA1017
(Linear) (Six-base) MAPSORT of: KIAA1017.seq Check: 6061 from: 1 to: 3045
KIAA1017.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1258 2011 2384 3045
Size: 1258 753 373 661
Fragments arranged by size:
1258 753 661 373
AfeI AGC'GCT
Cuts at: 0 733 3045
Size: 733 2312
AflIII A'CryG_T
Cuts at: 0 1935 3045
Size: 1935 1110
AlwNI CAG_nnn'CTG
Cuts at: 0 142 1642 1989 2951 3045
Size: 142 1500 347 962 94
Fragments arranged by size:
1500 962 347 142 94
ApaI G_GGCC'C
Cuts at: 0 2881 3045
Size: 2881 164
ApoI r'AATT_y
Cuts at: 0 587 801 925 1105 1908 1970 3045
Size: 587 214 124 180 803 62 1075
Fragments arranged by size:
1075 803 587 214 180 124 62
BamHI G'GATC_C
Cuts at: 0 511 2720 3045
Size: 511 2209 325
Fragments arranged by size:
2209 511 325
BanI G'GyrC_C
Cuts at: 0 880 1330 3045
Size: 880 450 1715
Fragments arranged by size:
1715 880 450
BanII G_rGCy'C
Cuts at: 0 1348 1373 2881 3045
Size: 1348 25 1508 164
Fragments arranged by size:
1508 1348 164 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 2 1058 1172 1439 1484 1846 3045
Size: 2 1056 114 267 45 362 1199
Fragments arranged by size:
1199 1056 362 267 114 45 2
BciVI GTATCCnnnnn_n'
Cuts at: 0 45 2067 3045
Size: 45 2022 978
Fragments arranged by size:
2022 978 45
BclI T'GATC_A
Cuts at: 0 81 498 3045
Size: 81 417 2547
Fragments arranged by size:
2547 417 81
BglII A'GATC_T
Cuts at: 0 2142 3045
Size: 2142 903
Bme1580I G_kGCm'C
Cuts at: 0 2881 3045
Size: 2881 164
BmrI ACTGGGnnnn_n'
Cuts at: 0 513 3045
Size: 513 2532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1454 2665 3045
Size: 1169 285 1211 380
Fragments arranged by size:
1211 1169 380 285
Bpu10I CC'TnA_GC
Cuts at: 0 1372 2867 3045
Size: 1372 1495 178
Fragments arranged by size:
1495 1372 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2407 2944 3045
Size: 2407 537 101
Fragments arranged by size:
2407 537 101
BsaI GGTCTCn'nnnn_
Cuts at: 0 1356 2722 3045
Size: 1356 1366 323
Fragments arranged by size:
1366 1356 323
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1045 1075 2802 2832 3045
Size: 1045 30 1727 30 213
Fragments arranged by size:
1727 1045 213 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 1250 1456 3045
Size: 436 814 206 1589
Fragments arranged by size:
1589 814 436 206
BseYI C'CCAG_C
Cuts at: 0 47 88 3035 3045
Size: 47 41 2947 10
Fragments arranged by size:
2947 47 41 10
BsiEI CG_ry'CG
Cuts at: 0 3021 3045
Size: 3021 24
BsiHKAI G_wGCw'C
Cuts at: 0 375 1373 2779 3045
Size: 375 998 1406 266
Fragments arranged by size:
1406 998 375 266
BsmI GAATG_Cn'
Cuts at: 0 1232 3045
Size: 1232 1813
Bsp1286I G_dGCh'C
Cuts at: 0 375 1348 1373 2779 2881 3045
Size: 375 973 25 1406 102 164
Fragments arranged by size:
1406 973 375 164 102 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1139 1426 2588 2959 3045
Size: 1139 287 1162 371 86
Fragments arranged by size:
1162 1139 371 287 86
BsrDI GCAATG_nn'
Cuts at: 0 809 1523 1755 1841 3045
Size: 809 714 232 86 1204
Fragments arranged by size:
1204 809 714 232 86
BsrGI T'GTAC_A
Cuts at: 0 195 3045
Size: 195 2850
BssSI C'ACGA_G
Cuts at: 0 1341 2778 3045
Size: 1341 1437 267
Fragments arranged by size:
1437 1341 267
BstAPI GCAn_nnn'nTGC
Cuts at: 0 142 564 3045
Size: 142 422 2481
Fragments arranged by size:
2481 422 142
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2472 2841 3045
Size: 2472 369 204
Fragments arranged by size:
2472 369 204
BstYI r'GATC_y
Cuts at: 0 511 2142 2720 3045
Size: 511 1631 578 325
Fragments arranged by size:
1631 578 511 325
Bsu36I CC'TnA_GG
Cuts at: 0 2962 3045
Size: 2962 83
BtgI C'CryG_G
Cuts at: 0 876 2732 2873 3045
Size: 876 1856 141 172
Fragments arranged by size:
1856 876 172 141
BtsI GCAGTG_nn'
Cuts at: 0 2444 2896 3045
Size: 2444 452 149
Fragments arranged by size:
2444 452 149
DraI TTT'AAA
Cuts at: 0 1101 3045
Size: 1101 1944
EarI CTCTTCn'nnn_
Cuts at: 0 24 750 1026 1118 1277 1391 2252 2649
Size: 24 726 276 92 159 114 861 397
Cuts at: 2649 3045
Size: 396
Fragments arranged by size:
861 726 397 396 276 159 114 92
24
EcoICRI GAG'CTC
Cuts at: 0 1371 3045
Size: 1371 1674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1258 1454 2011 2384 2665 3045
Size: 1169 89 196 557 373 281 380
Fragments arranged by size:
1169 557 380 373 281 196 89
EcoO109I rG'GnC_Cy
Cuts at: 0 1128 1533 2987 3045
Size: 1128 405 1454 58
Fragments arranged by size:
1454 1128 405 58
EcoRI G'AATT_C
Cuts at: 0 1105 1908 3045
Size: 1105 803 1137
Fragments arranged by size:
1137 1105 803
EcoRV GAT'ATC
Cuts at: 0 722 2292 2623 2958 3045
Size: 722 1570 331 335 87
Fragments arranged by size:
1570 722 335 331 87
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 640 848 1757 1820 2016 3045
Size: 640 208 909 63 196 1029
Fragments arranged by size:
1029 909 640 208 196 63
HaeII r_GCGC'y
Cuts at: 0 735 3045
Size: 735 2310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1039 1045 1071 1077 1335 1355 1367 1372
Size: 1039 6 26 6 258 20 12 5
Cuts at: 1372 1387 1404 1636 1668 2243 2275 2359 2391
Size: 15 17 232 32 575 32 84 32
Cuts at: 2391 2526 2558 3045
Size: 135 32 487
Fragments arranged by size:
1039 575 487 258 232 135 84 32
32 32 32 26 20 17 15 12
6 6 5
HincII GTy'rAC
Cuts at: 0 183 3045
Size: 183 2862
HindIII A'AGCT_T
Cuts at: 0 2041 2746 3045
Size: 2041 705 299
Fragments arranged by size:
2041 705 299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2399 2698 3045
Size: 2399 299 347
Fragments arranged by size:
2399 347 299
MslI CAynn'nnrTG
Cuts at: 0 551 692 1160 1779 2839 3045
Size: 551 141 468 619 1060 206
Fragments arranged by size:
1060 619 551 468 206 141
MspA1I CmG'CkG
Cuts at: 0 139 508 870 2373 2532 3045
Size: 139 369 362 1503 159 513
Fragments arranged by size:
1503 513 369 362 159 139
NcoI C'CATG_G
Cuts at: 0 876 2873 3045
Size: 876 1997 172
Fragments arranged by size:
1997 876 172
NspI r_CATG'y
Cuts at: 0 788 1778 1939 3006 3045
Size: 788 990 161 1067 39
Fragments arranged by size:
1067 990 788 161 39
PciI A'CATG_T
Cuts at: 0 1935 3045
Size: 1935 1110
PfoI T'CCnGG_A
Cuts at: 0 330 1487 3045
Size: 330 1157 1558
Fragments arranged by size:
1558 1157 330
PpuMI rG'GwC_Cy
Cuts at: 0 1128 1533 3045
Size: 1128 405 1512
Fragments arranged by size:
1512 1128 405
PsiI TTA'TAA
Cuts at: 0 421 2569 3045
Size: 421 2148 476
Fragments arranged by size:
2148 476 421
PspOMI G'GGCC_C
Cuts at: 0 2877 3045
Size: 2877 168
PstI C_TGCA'G
Cuts at: 0 843 2562 2599 3045
Size: 843 1719 37 446
Fragments arranged by size:
1719 843 446 37
PvuI CG_AT'CG
Cuts at: 0 3021 3045
Size: 3021 24
PvuII CAG'CTG
Cuts at: 0 139 508 870 2532 3045
Size: 139 369 362 1662 513
Fragments arranged by size:
1662 513 369 362 139
SacI G_AGCT'C
Cuts at: 0 1373 3045
Size: 1373 1672
SapI GCTCTTCn'nnn_
Cuts at: 0 2252 3045
Size: 2252 793
ScaI AGT'ACT
Cuts at: 0 1960 1999 3045
Size: 1960 39 1046
Fragments arranged by size:
1960 1046 39
SfcI C'TryA_G
Cuts at: 0 663 839 1528 2558 2595 2902 3045
Size: 663 176 689 1030 37 307 143
Fragments arranged by size:
1030 689 663 307 176 143 37
SmlI C'TyrA_G
Cuts at: 0 2386 2923 3045
Size: 2386 537 122
Fragments arranged by size:
2386 537 122
SpeI A'CTAG_T
Cuts at: 0 1459 3045
Size: 1459 1586
SphI G_CATG'C
Cuts at: 0 3006 3045
Size: 3006 39
SspI AAT'ATT
Cuts at: 0 1706 2673 3045
Size: 1706 967 372
Fragments arranged by size:
1706 967 372
StuI AGG'CCT
Cuts at: 0 761 2361 2496 2662 3045
Size: 761 1600 135 166 383
Fragments arranged by size:
1600 761 383 166 135
StyI C'CwwG_G
Cuts at: 0 876 1795 2873 3045
Size: 876 919 1078 172
Fragments arranged by size:
1078 919 876 172
TatI w'GTAC_w
Cuts at: 0 195 424 1958 1997 3045
Size: 195 229 1534 39 1048
Fragments arranged by size:
1534 1048 229 195 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2497 2529 2802 2834 3045
Size: 2497 32 273 32 211
Fragments arranged by size:
2497 273 211 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1935 2888 3045
Size: 1935 953 157
Fragments arranged by size:
1935 953 157
XmnI GAAnn'nnTTC
Cuts at: 0 339 1217 3045
Size: 339 878 1828
Fragments arranged by size:
1828 878 339
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApaI ApoI BamHI BanI
BanII BbsI BciVI BclI BglII Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrGI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtsI DraI EarI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
MmeI MslI MspA1I NcoI NspI PciI PfoI PpuMI
PsiI PspOMI PstI PvuI PvuII SacI SapI ScaI
SfcI SmlI SpeI SphI SspI StuI StyI TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbvCI BcgI BfrBI BglI BlpI
BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsgI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraIII DrdI EaeI
EagI EciI EcoNI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PspXI PsrI RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SrfI SwaI TaqII Tth111I XbaI XhoI XmaI
ZraI