Restriction map for pF1KA1006
##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       AccII       AclI        AcyI        AflII       
AgeI        AhdI        AocI        ApaI        AscI        
AspEI       AspI        AvrII       BbeI        BbrPI       
BbvCI       BcgI        BfrI        BlnI        BsaAI       
BsaHI       BsiEI       BsiWI       BspDI       BsrBI       
BsrFI       BssHII      BssSI       BstEII      BstPI       
BstUI       Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoO65I     EcoRI       
FseI        HgaI        Hin1I       KasI        KspI        
MluI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       Sse8387I    StuI        SwaI        
Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   67 enzymes) #
###################################

Acc65I       3548  GGTACC               G^GTACC
AccI         2844  GTATAC               GT^[AC][GT]AC
AccIII       1266  TCCGGA               T^CCGGA
AflIII       1516  ACATGT               A^C[GA][CT]GT
Alw44I       3413  GTGCAC               G^TGCAC
Aor51HI      2225  AGCGCT               AGC^GCT
ApaLI        3413  GTGCAC               G^TGCAC
AseI         2721  ATTAAT               AT^TAAT
AsnI         2721  ATTAAT               AT^TAAT
Asp700I        98  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3548  GGTACC               G^GTACC
AvaI          289  CTCGGG               C^[CT]CG[GA]G
AviII        3336  TGCGCA               TGC^GCA
BalI         1336  TGGCCA               TGG^CCA
BanII        3292  GAGCTC               G[GA]GC[CT]^C
BciVI        1318  GGATAC               GTATCC(6/5)
BclI          359  TGATCA               T^GATCA
BcnI          462  CCGGG                CC^[GC]GG
BglI         2974  GCCTGCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1943  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1943  GCTCAGC              GC^T[ACGT]AGC
BsaBI         769  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1266  TCCGGA               [AT]^CCGG[AT]
BseAI        1266  TCCGGA               T^CCGGA
BsmBI        3126  CGTCTC               CGTCTC(1/5)
BsmI         1853  GAATGC               GAATGC(1/-1)
BsoBI         289  CTCGGG               C^[CT]CG[GA]G
BspEI        1266  TCCGGA               T^CCGGA
BspLU11I     1516  ACATGT               A^CATGT
BspMI         943  ACCTGC               ACCTGC(4/8)
Bst1107I     2844  GTATAC               GTA^TAC
BstBI        2363  TTCGAA               TT^CGAA
BstZ17I      2844  GTATAC               GTA^TAC
CelII        1943  GCTCAGC              GC^T[ACGT]AGC
DraII        2343  GGGTCCT              [GA]G^G[ACGT]CC[CT]
Eco47III     2225  AGCGCT               AGC^GCT
EcoNI        3349  CCTGGACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     2343  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRV        1865  GATATC               GAT^ATC
EcoT22I      1027  ATGCAT               ATGCA^T
FbaI          359  TGATCA               T^GATCA
FspI         3336  TGCGCA               TGC^GCA
HincII       3150  GTTAAC               GT[CT]^[GA]AC
HindII       3150  GTTAAC               GT[CT]^[GA]AC
HpaI         3150  GTTAAC               GTT^AAC
KpnI         3548  GGTACC               GGTAC^C
MaeII        2304  ACGT                 A^CGT
MamI          769  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1336  TGGCCA               TGG^CCA
MroI         1266  TCCGGA               T^CCGGA
MscI         1336  TGGCCA               TGG^CCA
NciI          462  CCGGG                CC^[GC]GG
NcoI         1211  CCATGG               C^CATGG
NsiI         1027  ATGCAT               ATGCA^T
NspV         2363  TTCGAA               TT^CGAA
PflMI        1339  CCAATCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2343  GGGTCCT              [GA]G^G[AT]CC[CT]
PshBI        2721  ATTAAT               AT^TAAT
PvuII         335  CAGCTG               CAG^CTG
SacI         3292  GAGCTC               GAGCT^C
SapI         3289  GAAGAGC              GCTCTTC(1/4)
SfuI         2363  TTCGAA               TT^CGAA
SpeI         1927  ACTAGT               A^CTAGT
Tsp45I       2054  GTGAC                ^GT[GC]AC
Van91I       1339  CCAATCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2446  TCTAGA               T^CTAGA
XmnI           98  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI        1450  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2970  CAGTGCCTG           
BamHI        2162  GGATCC               G^GATCC
             2751  GGATCC              
BanI          370  GGTGCC               G^G[CT][GA]CC
             3548  GGTACC              
BglII        1152  AGATCT               A^GATCT
             1734  AGATCT              
BsaI          294  GAGACC               GGTCTC(1/5)
             2078  GGTCTC              
Bsp1407I      633  TGTACA               T^GTACA
             1457  TGTACA              
BsrDI        2103  CATTGC               GCAATG(2/0)
             1976  GCAATG              
BsrGI         633  TGTACA               T^GTACA
             1457  TGTACA              
BstXI         804  CCATTCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2250  CCATGTAAATGG        
DsaI         1211  CCATGG               C^C[GA][CT]GG
             2074  CCACGG              
EaeI         1336  TGGCCA               [CT]^GGCC[GA]
             1843  CGGCCA              
EarI         3289  GAAGAG               CTCTTC(1/4)
             3460  GAAGAG              
FauI         3326  CCCGC                CCCGC(4/6)
              118  GCGGG               
HaeII        2225  AGCGCT               [GA]GCGC^[CT]
             3379  GGCGCT              
HindIII       998  AAGCTT               A^AGCTT
             3305  AAGCTT              
Ksp632I      3289  GAAGAG               CTCTTC(1/4)
             3460  GAAGAG              
MspA1I        335  CAGCTG               C[AC]G^C[GT]G
             3145  CAGCGG              
SexAI         297  ACCTGGT              A^CC[AT]GGT
             3354  ACCAGGT             
SmlI         2221  CTTGAG               C^T[CT][GA]AG
             3247  CTCAAG              
SphI         2120  GCATGC               GCATG^C
             2983  GCATGC              
SspBI         633  TGTACA               T^GTACA
             1457  TGTACA              
TaqI         2085  TCGA                 T^CGA
             2364  TCGA                
TthHB8I      2085  TCGA                 T^CGA
             2364  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BbsI        BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MfeI        MflI        MnlI        MseI        
MslI        MspI        MunI        MvaI        MwoI        
NdeI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        RcaI        RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SspI        StyI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XcmI        XhoII       
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