Restriction map for pF1KA1006
 (Linear) (Six-base) MAPSORT of: KIAA1006.seq  Check: 2770  from: 1  to: 3558



KIAA1006.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AccI GT'mk_AC

Cuts at:      0    2845    3558 
   Size:       2845     713
 
Acc65I G'GTAC_C

Cuts at:      0    3548    3558 
   Size:       3548      10
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1660    3558 
   Size:       1660    1898
 
AfeI AGC'GCT

Cuts at:      0    2227    3558 
   Size:       2227    1331
 
AflIII A'CryG_T

Cuts at:      0    1516    3558 
   Size:       1516    2042
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1455    2975    3558 
   Size:       1455    1520     583

  Fragments arranged by size: 

                1520    1455     583
 
ApaLI G'TGCA_C

Cuts at:      0    3413    3558 
   Size:       3413     145
 
ApoI r'AATT_y

Cuts at:      0     146     454    1075    1111    1420    1624    2430    3140 
   Size:        146     308     621      36     309     204     806     710

Cuts at:   3140    3558 
   Size:        418

  Fragments arranged by size: 

                 806     710     621     418     309     308     204     146
                  36
 
AseI AT'TA_AT

Cuts at:      0    2722    3558 
   Size:       2722     836
 
AvaI C'yCGr_G

Cuts at:      0     289    3558 
   Size:        289    3269
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     683     716    3558 
   Size:        683      33    2842

  Fragments arranged by size: 

                2842     683      33
 
BamHI G'GATC_C

Cuts at:      0    2162    2751    3558 
   Size:       2162     589     807

  Fragments arranged by size: 

                2162     807     589
 
BanI G'GyrC_C

Cuts at:      0     370    3548    3558 
   Size:        370    3178      10

  Fragments arranged by size: 

                3178     370      10
 
BanII G_rGCy'C

Cuts at:      0    3296    3558 
   Size:       3296     262
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1014    1541    1843    2014    2358    2871    3022    3558 
   Size:       1014     527     302     171     344     513     151     536

  Fragments arranged by size: 

                1014     536     527     513     344     302     171     151
            
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1312    3558 
   Size:       1312    2246
 
BclI T'GATC_A

Cuts at:      0     359    3558 
   Size:        359    3199
 
BfrBI ATG'CAT

Cuts at:      0    1029    3558 
   Size:       1029    2529
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2980    3558 
   Size:       2980     578
 
BglII A'GATC_T

Cuts at:      0    1152    1734    3558 
   Size:       1152     582    1824

  Fragments arranged by size: 

                1824    1152     582
 
BlpI GC'TnA_GC

Cuts at:      0    1944    3558 
   Size:       1944    1614
 
Bme1580I G_kGCm'C

Cuts at:      0    3417    3558 
   Size:       3417     141
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2367    3558 
   Size:       2367    1191
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3262    3310    3558 
   Size:       3262      48     248

  Fragments arranged by size: 

                3262     248      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1165    1844    2448    3407    3558 
   Size:       1165     679     604     959     151

  Fragments arranged by size: 

                1165     959     679     604     151
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2242    3232    3558 
   Size:       2242     990     326

  Fragments arranged by size: 

                2242     990     326
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     288    2084    3558 
   Size:        288    1796    1474

  Fragments arranged by size: 

                1796    1474     288
 
BsaBI GATnn'nnATC

Cuts at:      0     773    3558 
   Size:        773    2785
 
BsaWI w'CCGG_w

Cuts at:      0    1266    3558 
   Size:       1266    2292
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     285     315    1707    1737    2532    2562    3558 
   Size:        285      30    1392      30     795      30     996

  Fragments arranged by size: 

                1392     996     795     285      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      36    1364    2072    3558 
   Size:         36    1328     708    1486

  Fragments arranged by size: 

                1486    1328     708      36
 
BseYI C'CCAG_C

Cuts at:      0    1745    2339    3558 
   Size:       1745     594    1219

  Fragments arranged by size: 

                1745    1219     594
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     819    2250    3509    3558 
   Size:        353     466    1431    1259      49

  Fragments arranged by size: 

                1431    1259     466     353      49
 
BsiHKAI G_wGCw'C

Cuts at:      0    1371    1780    3296    3417    3558 
   Size:       1371     409    1516     121     141

  Fragments arranged by size: 

                1516    1371     409     141     121
 
BsmI GAATG_Cn'

Cuts at:      0    1859    3558 
   Size:       1859    1699
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3132    3558 
   Size:       3132     426
 
Bsp1286I G_dGCh'C

Cuts at:      0    1371    1780    3296    3417    3558 
   Size:       1371     409    1516     121     141

  Fragments arranged by size: 

                1516    1371     409     141     121
 
BspEI T'CCGG_A

Cuts at:      0    1266    3558 
   Size:       1266    2292
 
BspHI T'CATG_A

Cuts at:      0     777    1221    3008    3558 
   Size:        777     444    1787     550

  Fragments arranged by size: 

                1787     777     550     444
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     952    3558 
   Size:        952    2606
 
BsrDI GCAATG_nn'

Cuts at:      0    1983    2102    3558 
   Size:       1983     119    1456

  Fragments arranged by size: 

                1983    1456     119
 
BsrGI T'GTAC_A

Cuts at:      0     633    1457    3558 
   Size:        633     824    2101

  Fragments arranged by size: 

                2101     824     633
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1252    2975    3421    3558 
   Size:       1252    1723     446     137

  Fragments arranged by size: 

                1723    1252     446     137
 
BstBI TT'CG_AA

Cuts at:      0    2364    3558 
   Size:       2364    1194
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     811    2257    3558 
   Size:        811    1446    1301

  Fragments arranged by size: 

                1446    1301     811
 
BstYI r'GATC_y

Cuts at:      0    1152    1734    2162    2751    3558 
   Size:       1152     582     428     589     807

  Fragments arranged by size: 

                1152     807     589     582     428
 
BstZ17I GTA'TAC

Cuts at:      0    2846    3558 
   Size:       2846     712
 
BtgI C'CryG_G

Cuts at:      0    1211    2074    3558 
   Size:       1211     863    1484

  Fragments arranged by size: 

                1484    1211     863
 
BtsI GCAGTG_nn'

Cuts at:      0      63      75    2262    2884    2976    3558 
   Size:         63      12    2187     622      92     582

  Fragments arranged by size: 

                2187     622     582      92      63      12
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1876    1911    2936    2971    3558 
   Size:       1876      35    1025      35     587

  Fragments arranged by size: 

                1876    1025     587      35      35
 
EaeI y'GGCC_r

Cuts at:      0    1336    1843    3558 
   Size:       1336     507    1715

  Fragments arranged by size: 

                1715    1336     507
 
EarI CTCTTCn'nnn_

Cuts at:      0    3284    3455    3558 
   Size:       3284     171     103

  Fragments arranged by size: 

                3284     171     103
 
EcoICRI GAG'CTC

Cuts at:      0    3294    3558 
   Size:       3294     264
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1165    1660    1844    2448    3407    3558 
   Size:       1165     495     184     604     959     151

  Fragments arranged by size: 

                1165     959     604     495     184     151
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3353    3558 
   Size:       3353     205
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2344    3558 
   Size:       2344    1214
 
EcoRV GAT'ATC

Cuts at:      0    1867    3558 
   Size:       1867    1691
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     869    1894    2294    3558 
   Size:        869    1025     400    1264

  Fragments arranged by size: 

                1264    1025     869     400
 
FspI TGC'GCA

Cuts at:      0    3338    3558 
   Size:       3338     220
 
HaeII r_GCGC'y

Cuts at:      0    2229    3383    3558 
   Size:       2229    1154     175

  Fragments arranged by size: 

                2229    1154     175
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     563     595     700     732    1914    1946    3558 
   Size:        563      32     105      32    1182      32    1612

  Fragments arranged by size: 

                1612    1182     563     105      32      32      32
 
HincII GTy'rAC

Cuts at:      0    3152    3558 
   Size:       3152     406
 
HindIII A'AGCT_T

Cuts at:      0     998    3305    3558 
   Size:        998    2307     253

  Fragments arranged by size: 

                2307     998     253
 
HpaI GTT'AAC

Cuts at:      0    3152    3558 
   Size:       3152     406
 
KpnI G_GTAC'C

Cuts at:      0    3552    3558 
   Size:       3552       6
 
MfeI C'AATT_G

Cuts at:      0     596     764    1888    3558 
   Size:        596     168    1124    1670

  Fragments arranged by size: 

                1670    1124     596     168
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     495    2299    2544    3023    3558 
   Size:        495    1804     245     479     535

  Fragments arranged by size: 

                1804     535     495     479     245
 
MscI TGG'CCA

Cuts at:      0    1338    3558 
   Size:       1338    2220
 
MslI CAynn'nnrTG

Cuts at:      0     326     809    1406    1552    2255    3013    3512    3558 
   Size:        326     483     597     146     703     758     499      46

  Fragments arranged by size: 

                 758     703     597     499     483     326     146      46
            
 
MspA1I CmG'CkG

Cuts at:      0     337    3147    3558 
   Size:        337    2810     411

  Fragments arranged by size: 

                2810     411     337
 
NcoI C'CATG_G

Cuts at:      0    1211    3558 
   Size:       1211    2347
 
NdeI CA'TA_TG

Cuts at:      0    1608    1950    2736    3558 
   Size:       1608     342     786     822

  Fragments arranged by size: 

                1608     822     786     342
 
NsiI A_TGCA'T

Cuts at:      0    1031    3558 
   Size:       1031    2527
 
NspI r_CATG'y

Cuts at:      0    1241    1520    2124    2987    3192    3511    3558 
   Size:       1241     279     604     863     205     319      47

  Fragments arranged by size: 

                1241     863     604     319     279     205      47
 
PciI A'CATG_T

Cuts at:      0    1516    3558 
   Size:       1516    2042
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1345    3558 
   Size:       1345    2213
 
PfoI T'CCnGG_A

Cuts at:      0    1860    2346    3348    3558 
   Size:       1860     486    1002     210

  Fragments arranged by size: 

                1860    1002     486     210
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2277    2309    3558 
   Size:       2277      32    1249

  Fragments arranged by size: 

                2277    1249      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2344    3558 
   Size:       2344    1214
 
PsiI TTA'TAA

Cuts at:      0    1622    3558 
   Size:       1622    1936
 
PstI C_TGCA'G

Cuts at:      0     129    1451    3474    3558 
   Size:        129    1322    2023      84

  Fragments arranged by size: 

                2023    1322     129      84
 
PvuII CAG'CTG

Cuts at:      0     337    3558 
   Size:        337    3221
 
SacI G_AGCT'C

Cuts at:      0    3296    3558 
   Size:       3296     262
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3284    3558 
   Size:       3284     274
 
ScaI AGT'ACT

Cuts at:      0    1049    1283    2015    3558 
   Size:       1049     234     732    1543

  Fragments arranged by size: 

                1543    1049     732     234
 
SexAI A'CCwGG_T

Cuts at:      0     297    3354    3558 
   Size:        297    3057     204

  Fragments arranged by size: 

                3057     297     204
 
SfcI C'TryA_G

Cuts at:      0     125     343     695    1292    1447    3181    3470    3558 
   Size:        125     218     352     597     155    1734     289      88

  Fragments arranged by size: 

                1734     597     352     289     218     155     125      88
            
 
SmlI C'TyrA_G

Cuts at:      0    2221    3247    3558 
   Size:       2221    1026     311

  Fragments arranged by size: 

                2221    1026     311
 
SpeI A'CTAG_T

Cuts at:      0    1927    3558 
   Size:       1927    1631
 
SphI G_CATG'C

Cuts at:      0    2124    2987    3558 
   Size:       2124     863     571

  Fragments arranged by size: 

                2124     863     571
 
SspI AAT'ATT

Cuts at:      0     549     971    1583    3558 
   Size:        549     422     612    1975

  Fragments arranged by size: 

                1975     612     549     422
 
StyI C'CwwG_G

Cuts at:      0     374    1211    1362    3558 
   Size:        374     837     151    2196

  Fragments arranged by size: 

                2196     837     374     151
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2704    2723    3558 
   Size:       2704      19     835

  Fragments arranged by size: 

                2704     835      19
 
TatI w'GTAC_w

Cuts at:      0     633    1047    1281    1457    2013    3558 
   Size:        633     414     234     176     556    1545

  Fragments arranged by size: 

                1545     633     556     414     234     176
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     285     317    3558 
   Size:        285      32    3241

  Fragments arranged by size: 

                3241     285      32
 
XbaI T'CTAG_A

Cuts at:      0    2446    3558 
   Size:       2446    1112
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     325    2577    2741    3558 
   Size:        325    2252     164     817

  Fragments arranged by size: 

                2252     817     325     164
 
XmnI GAAnn'nnTTC

Cuts at:      0     102    3558 
   Size:        102    3456
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII    AlwNI    ApaLI     ApoI
     AseI     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrGI   BstAPI    BstBI    BstXI    BstYI  BstZ17I     BtgI
     BtsI    CspCI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NsiI     NspI     PciI    PflMI     PfoI     PpiI    PpuMI     PsiI
     PstI    PvuII     SacI     SapI     ScaI    SexAI     SfcI     SmlI
     SpeI     SphI     SspI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI     AscI    AsiSI    AvrII     BbeI    BbvCI     BcgI
    BmgBI     BmtI   Bpu10I    BsaAI    BsaHI    BsiEI    BsiWI    BsrBI
    BsrFI   BssHII    BssSI   BstEII   Bsu36I    BtgZI     ClaI     DraI
   DraIII     DrdI     EagI     EciI    EcoRI     FseI    FspAI     KasI
     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PmeI     PmlI    PshAI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SrfI     StuI     SwaI  Tth111I     XhoI
     XmaI     ZraI
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