Restriction map for pF1KA0983
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# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AvaI        
AviII       AvrII       BalI        BbeI        BbrPI       
BbvCI       BcgI        BcnI        BfrI        BglI        
BglII       BlnI        BsaAI       BsaHI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstUI       BstZ17I     Cfr10I      
CpoI        DraIII      DrdI        EaeI        EagI        
EclXI       Eco47III    Eco52I      EcoRI       EcoT22I     
FseI        FspI        HgaI        Hin1I       HpaI        
KasI        KpnI        KspI        MluI        MluNI       
MroI        MscI        MslI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI         1951  GAATTT               [GA]^AATT[CT]
AhdI         1177  GACTTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2026  CCTCAGG              CC^T[ACGT]AGG
ApaI         1942  GGGCCC               GGGCC^C
ApoI         1951  GAATTT               [GA]^AATT[CT]
AspEI        1177  GACTTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1192  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         263  GGATCC               G^GATCC
BanI         1028  GGCACC               G^G[CT][GA]CC
BbsI         1437  GAAGAC               GAAGAC(2/6)
BciVI        1212  GTATCC               GTATCC(6/5)
BclI         1154  TGATCA               T^GATCA
BpuAI        1437  GAAGAC               GAAGAC(2/6)
BsaBI         370  GATGCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         723  TCCGGT               [AT]^CCGG[AT]
BspDI          77  ATCGAT               AT^CGAT
BssSI         349  CACGAG               CACGAG(-5/-1)
BstEII       1312  GGTCACC              G^GT[ACGT]ACC
BstPI        1312  GGTCACC              G^GT[ACGT]ACC
Bsu36I       2026  CCTCAGG              CC^T[ACGT]AGG
ClaI           77  ATCGAT               AT^CGAT
DraI         2011  TTTAAA               TTT^AAA
Eam1105I     1177  GACTTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2026  CCTCAGG              CC^T[ACGT]AGG
EcoNI         417  CCTACAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1312  GGTCACC              G^GT[ACGT]ACC
EcoRV         514  GATATC               GAT^ATC
FbaI         1154  TGATCA               T^GATCA
HincII       1863  GTTGAC               GT[CT]^[GA]AC
HindII       1863  GTTGAC               GT[CT]^[GA]AC
HindIII       298  AAGCTT               A^AGCTT
MamI          370  GATGCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1571  CAATTG               C^AATTG
MunI         1571  CAATTG               C^AATTG
PflFI        1192  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
StuI          839  AGGCCT               AGG^CCT
SwaI         2010  ATTTAAAT             ATTT^AAAT
Tth111I      1192  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1607  CCAAAGACATTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AvaII         138  GGACC                G^G[AT]CC
             1990  GGTCC               
BanII          53  GAGCCC               G[GA]GC[CT]^C
             1942  GGGCCC              
BlpI          355  GCTAAGC              GC^T[ACGT]AGC
             1398  GCTGAGC             
Bpu1102I      355  GCTAAGC              GC^T[ACGT]AGC
             1398  GCTGAGC             
BstXI         434  CCAAATGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1406  CCAGCCACATGG        
CelII         355  GCTAAGC              GC^T[ACGT]AGC
             1398  GCTGAGC             
HapII         724  CCGG                 C^CGG
              972  CCGG                
HpaII         724  CCGG                 C^CGG
              972  CCGG                
MaeII         567  ACGT                 A^CGT
              853  ACGT                
MspI          724  CCGG                 C^CGG
              972  CCGG                
PpuMI         137  GGGACCC              [GA]G^G[AT]CC[CT]
             1989  GGGTCCT             
SapI          216  GAAGAGC              GCTCTTC(1/4)
             1980  GCTCTTC             
SmlI          646  CTCAAG               C^T[CT][GA]AG
             1303  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
BbvI        BfaI        BmyI        BpmI        BsaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BspMI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HhaI        HinP1I      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       PleI        PstI        PvuII       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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