(Circular) (Six-base) MAPSORT of: pF1KA0931.seq Check: 4217 from: 1 to: 6881 pF1KA0931 6881 bp With 182 enzymes: SgfI * June 20, 2008 10:20 .. AccI GT'mk_AC Cuts at: 2060 3413 3919 2060 Size: 1353 506 5022 Fragments arranged by size: 5022 1353 506 Acc65I G'GTAC_C Cuts at: 640 3897 640 Size: 3257 3624 AclI AA'CG_TT Cuts at: 6721 6721 Size: 6881 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 403 1030 1211 1230 1822 2643 4706 5138 5491 Size: 627 181 19 592 821 2063 432 353 Cuts at: 5491 6153 403 Size: 662 1131 Fragments arranged by size: 2063 1131 821 662 627 592 432 353 181 19 AflIII A'CryG_T Cuts at: 4233 6033 6314 4233 Size: 1800 281 4800 Fragments arranged by size: 4800 1800 281 AleI CACnn'nnGTG Cuts at: 1435 3685 1435 Size: 2250 4631 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1326 2637 3571 1326 Size: 1311 934 4636 Fragments arranged by size: 4636 1311 934 AlwNI CAG_nnn'CTG Cuts at: 2893 3061 4186 5624 2893 Size: 168 1125 1438 4150 Fragments arranged by size: 4150 1438 1125 168 ApaLI G'TGCA_C Cuts at: 2946 5719 2946 Size: 2773 4108 ApoI r'AATT_y Cuts at: 997 1229 1253 1736 3885 6234 6490 997 Size: 232 24 483 2149 2349 256 1388 Fragments arranged by size: 2349 2149 1388 483 256 232 24 AseI AT'TA_AT Cuts at: 20 20 Size: 6881 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6881 AvaI C'yCGr_G Cuts at: 161 889 1822 3814 3901 161 Size: 728 933 1992 87 3141 Fragments arranged by size: 3141 1992 933 728 87 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2425 2458 2425 Size: 33 6848 BamHI G'GATC_C Cuts at: 2789 3518 3906 2789 Size: 729 388 5764 Fragments arranged by size: 5764 729 388 BanI G'GyrC_C Cuts at: 640 3897 4311 4559 4594 640 Size: 3257 414 248 35 2927 Fragments arranged by size: 3257 2927 414 248 35 BanII G_rGCy'C Cuts at: 771 1088 2387 2524 3047 3827 3851 3895 4925 Size: 317 1299 137 523 780 24 44 1030 Cuts at: 4925 771 Size: 2727 Fragments arranged by size: 2727 1299 1030 780 523 317 137 44 24 BbeI G_GCGC'C Cuts at: 4315 4563 4315 Size: 248 6633 BbsI GAAGACnn'nnnn_ Cuts at: 2649 3531 2649 Size: 882 5999 BbvCI CC'TCA_GC Cuts at: 1522 1522 Size: 6881 BciVI GTATCCnnnnn_n' Cuts at: 4779 5835 6881 4779 Size: 1056 1046 4779 Fragments arranged by size: 4779 1056 1046 BclI T'GATC_A Cuts at: 2208 4401 2208 Size: 2193 4688 BglI GCCn_nnn'nGGC Cuts at: 3252 3252 Size: 6881 BglII A'GATC_T Cuts at: 1067 4396 6433 1067 Size: 3329 2037 1515 Fragments arranged by size: 3329 2037 1515 BlpI GC'TnA_GC Cuts at: 617 759 3993 617 Size: 142 3234 3505 Fragments arranged by size: 3505 3234 142 Bme1580I G_kGCm'C Cuts at: 2645 2950 3647 3692 4506 4599 5723 2645 Size: 305 697 45 814 93 1124 3803 Fragments arranged by size: 3803 1124 814 697 305 93 45 BmrI ACTGGGnnnn_n' Cuts at: 1674 4093 4174 4509 6653 1674 Size: 2419 81 335 2144 1902 Fragments arranged by size: 2419 2144 1902 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5248 5248 Size: 6881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 362 1432 2277 2686 3306 3463 362 Size: 1070 845 409 620 157 3780 Fragments arranged by size: 3780 1070 845 620 409 157 Bpu10I CC'TnA_GC Cuts at: 902 1522 3101 3821 902 Size: 620 1579 720 3962 Fragments arranged by size: 3962 1579 720 620 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1770 4057 4936 5650 5948 1770 Size: 2287 879 714 298 2703 Fragments arranged by size: 2703 2287 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 374 2363 6585 374 Size: 1989 4222 670 Fragments arranged by size: 4222 1989 670 BsaAI yAC'GTr Cuts at: 1437 2606 2913 4234 1437 Size: 1169 307 1321 4084 Fragments arranged by size: 4084 1321 1169 307 BsaBI GATnn'nnATC Cuts at: 6 4395 6432 6 Size: 4389 2037 455 Fragments arranged by size: 4389 2037 455 BsaHI Gr'CG_yC Cuts at: 4312 4560 5262 6758 4312 Size: 248 702 1496 4435 Fragments arranged by size: 4435 1496 702 248 BsaWI w'CCGG_w Cuts at: 4070 4591 5269 5680 5827 6101 4070 Size: 521 678 411 147 274 4850 Fragments arranged by size: 4850 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 371 401 371 Size: 30 6851 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 246 261 264 267 493 907 2395 2514 2695 Size: 15 3 3 226 414 1488 119 181 Cuts at: 2695 3871 246 Size: 1176 3256 Fragments arranged by size: 3256 1488 1176 414 226 181 119 15 3 3 BseYI C'CCAG_C Cuts at: 447 599 851 1609 1770 2763 3214 4306 5064 Size: 152 252 758 161 993 451 1092 758 Cuts at: 5064 5729 6137 447 Size: 665 408 1191 Fragments arranged by size: 1191 1092 993 758 758 665 451 408 252 161 152 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1969 3009 1969 Size: 1040 5841 BsiEI CG_ry'CG Cuts at: 104 4423 4469 5699 104 Size: 4319 46 1230 1286 Fragments arranged by size: 4319 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 1088 1814 2387 2950 3895 4673 4863 5723 1088 Size: 726 573 563 945 778 190 860 2246 Fragments arranged by size: 2246 945 860 778 726 573 563 190 Bsp1286I G_dGCh'C Cuts at: 771 1088 1814 2387 2524 2645 2950 3047 3647 Size: 317 726 573 137 121 305 97 600 Cuts at: 3647 3692 3827 3851 3895 4506 4599 4673 4863 Size: 45 135 24 44 611 93 74 190 Cuts at: 4863 4925 5723 771 Size: 62 798 1929 Fragments arranged by size: 1929 798 726 611 600 573 317 305 190 137 135 121 97 93 74 62 45 44 24 BspHI T'CATG_A Cuts at: 1110 3759 5313 1110 Size: 2649 1554 2678 Fragments arranged by size: 2678 2649 1554 BspMI ACCTGCnnnn'nnnn_ Cuts at: 652 1652 2755 3931 4145 4447 4828 652 Size: 1000 1103 1176 214 302 381 2705 Fragments arranged by size: 2705 1176 1103 1000 381 302 214 BsrBI CCG'CTC Cuts at: 50 2530 3252 5173 5227 6711 6876 50 Size: 2480 722 1921 54 1484 165 55 Fragments arranged by size: 2480 1921 1484 722 165 55 54 BsrDI GCAATG_nn' Cuts at: 123 4793 123 Size: 4670 2211 BsrFI r'CCGG_y Cuts at: 3809 4879 5060 3809 Size: 1070 181 5630 Fragments arranged by size: 5630 1070 181 BsrGI T'GTAC_A Cuts at: 2770 2770 Size: 6881 BssSI C'ACGA_G Cuts at: 876 1854 5152 5860 876 Size: 978 3298 708 1897 Fragments arranged by size: 3298 1897 978 708 BstAPI GCAn_nnn'nTGC Cuts at: 696 4258 696 Size: 3562 3319 BstBI TT'CG_AA Cuts at: 5242 5242 Size: 6881 BstEII G'GTnAC_C Cuts at: 2742 2742 Size: 6881 BstXI CCAn_nnnn'nTGG Cuts at: 3729 3729 Size: 6881 BstYI r'GATC_y Cuts at: 1 870 1067 1440 1470 1629 1833 2048 2789 Size: 869 197 373 30 159 204 215 741 Cuts at: 2789 3438 3518 3906 4373 4396 4731 4977 5283 Size: 649 80 388 467 23 335 246 306 Cuts at: 5283 5295 5381 6433 1 Size: 12 86 1052 449 Fragments arranged by size: 1052 869 741 649 467 449 388 373 335 306 246 215 204 197 159 86 80 30 23 12 Bsu36I CC'TnA_GG Cuts at: 1259 1259 Size: 6881 BtgI C'CryG_G Cuts at: 866 1394 2262 866 Size: 528 868 5485 Fragments arranged by size: 5485 868 528 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 834 3004 4831 5012 5175 834 Size: 2170 1827 181 163 2540 Fragments arranged by size: 2540 2170 1827 181 163 BtsI GCAGTG_nn' Cuts at: 230 1595 2407 2555 3132 3156 3183 3191 3227 Size: 1365 812 148 577 24 27 8 36 Cuts at: 3227 3420 4124 230 Size: 193 704 2987 Fragments arranged by size: 2987 1365 812 704 577 193 148 36 27 24 8 DraI TTT'AAA Cuts at: 3880 3880 Size: 6881 DraIII CAC_nnn'GTG Cuts at: 1540 1540 Size: 6881 DrdI GACnn_nn'nnGTC Cuts at: 4587 5931 4587 Size: 1344 5537 EaeI y'GGCC_r Cuts at: 806 2508 3677 4466 4640 5031 5058 6403 806 Size: 1702 1169 789 174 391 27 1345 1284 Fragments arranged by size: 1702 1345 1284 1169 789 391 174 27 EagI C'GGCC_G Cuts at: 4466 4466 Size: 6881 EarI CTCTTCn'nnn_ Cuts at: 264 270 285 345 422 916 1879 2423 3827 Size: 6 15 60 77 494 963 544 1404 Cuts at: 3827 4904 5114 264 Size: 1077 210 2031 Fragments arranged by size: 2031 1404 1077 963 544 494 210 77 60 15 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1527 2536 5089 5831 5977 6691 1527 Size: 1009 2553 742 146 714 1717 Fragments arranged by size: 2553 1717 1009 742 714 146 EcoICRI GAG'CTC Cuts at: 1086 2385 3893 1086 Size: 1299 1508 4074 Fragments arranged by size: 4074 1508 1299 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 362 403 1030 1211 1230 1432 1822 2277 2643 Size: 41 627 181 19 202 390 455 366 Cuts at: 2643 2686 3306 3463 4706 5138 5491 6153 362 Size: 43 620 157 1243 432 353 662 1090 Fragments arranged by size: 1243 1090 662 627 620 455 432 390 366 353 202 181 157 43 41 19 EcoNI CCTnn'n_nnAGG Cuts at: 892 2668 892 Size: 1776 5105 EcoO109I rG'GnC_Cy Cuts at: 1014 2673 3058 3219 4020 1014 Size: 1659 385 161 801 3875 Fragments arranged by size: 3875 1659 801 385 161 EcoRI G'AATT_C Cuts at: 1736 3885 1736 Size: 2149 4732 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4900 4900 Size: 6881 FspI TGC'GCA Cuts at: 469 4662 469 Size: 4193 2688 FspAI rTGC'GCAy Cuts at: 469 469 Size: 6881 HaeII r_GCGC'y Cuts at: 624 3258 3300 4315 4563 5793 6564 624 Size: 2634 42 1015 248 1230 771 941 Fragments arranged by size: 2634 1230 1015 941 771 248 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 371 403 424 456 1462 1494 1695 1727 4723 Size: 32 21 32 1006 32 201 32 2996 Cuts at: 4723 4753 4755 4785 371 Size: 30 2 30 2467 Fragments arranged by size: 2996 2467 1006 201 32 32 32 32 30 30 21 2 HincII GTy'rAC Cuts at: 2249 2444 3920 2249 Size: 195 1476 5210 Fragments arranged by size: 5210 1476 195 HindIII A'AGCT_T Cuts at: 1942 1942 Size: 6881 KasI G'GCGC_C Cuts at: 4311 4559 4311 Size: 248 6633 KpnI G_GTAC'C Cuts at: 644 3901 644 Size: 3257 3624 MfeI C'AATT_G Cuts at: 1912 1912 Size: 6881 MluI A'CGCG_T Cuts at: 6314 6314 Size: 6881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1598 1822 2030 5640 5824 1598 Size: 224 208 3610 184 2655 Fragments arranged by size: 3610 2655 224 208 184 MscI TGG'CCA Cuts at: 2510 3679 4642 2510 Size: 1169 963 4749 Fragments arranged by size: 4749 1169 963 MslI CAynn'nnrTG Cuts at: 1435 1781 3673 3685 3727 4870 4997 1435 Size: 346 1892 12 42 1143 127 3319 Fragments arranged by size: 3319 1892 1143 346 127 42 12 MspA1I CmG'CkG Cuts at: 603 1521 2823 2932 3992 4306 4666 5450 5695 Size: 918 1302 109 1060 314 360 784 245 Cuts at: 5695 603 Size: 1789 Fragments arranged by size: 1789 1302 1060 918 784 360 314 245 109 NaeI GCC'GGC Cuts at: 3811 5062 3811 Size: 1251 5630 NarI GG'CG_CC Cuts at: 4312 4560 4312 Size: 248 6633 NcoI C'CATG_G Cuts at: 1394 2262 1394 Size: 868 6013 NgoMIV G'CCGG_C Cuts at: 3809 5060 3809 Size: 1251 5630 NspI r_CATG'y Cuts at: 1366 2361 3499 3552 3934 4434 6037 1366 Size: 995 1138 53 382 500 1603 2210 Fragments arranged by size: 2210 1603 1138 995 500 382 53 PciI A'CATG_T Cuts at: 6033 6033 Size: 6881 PflMI CCAn_nnn'nTGG Cuts at: 2268 2517 2268 Size: 249 6632 PmeI GTTT'AAAC Cuts at: 3880 3880 Size: 6881 PmlI CAC'GTG Cuts at: 1437 2606 1437 Size: 1169 5712 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5327 5359 5327 Size: 32 6849 PpuMI rG'GwC_Cy Cuts at: 2673 3058 2673 Size: 385 6496 PspXI vC'TCGA_Gb Cuts at: 889 1822 3814 889 Size: 933 1992 3956 Fragments arranged by size: 3956 1992 933 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 141 173 141 Size: 32 6849 PstI C_TGCA'G Cuts at: 2039 2402 3262 3430 3928 4613 2039 Size: 363 860 168 498 685 4307 Fragments arranged by size: 4307 860 685 498 363 168 PvuI CG_AT'CG Cuts at: 104 104 Size: 6881 PvuII CAG'CTG Cuts at: 603 1521 2932 4306 4666 603 Size: 918 1411 1374 360 2818 Fragments arranged by size: 2818 1411 1374 918 360 RsrII CG'GwC_CG Cuts at: 3842 5076 3842 Size: 1234 5647 SacI G_AGCT'C Cuts at: 1088 2387 3895 1088 Size: 1299 1508 4074 Fragments arranged by size: 4074 1508 1299 SalI G'TCGA_C Cuts at: 3918 3918 Size: 6881 SapI GCTCTTCn'nnn_ Cuts at: 4904 5114 4904 Size: 210 6671 SbfI CC_TGCA'GG Cuts at: 3928 3928 Size: 6881 ScaI AGT'ACT Cuts at: 6398 6398 Size: 6881 SexAI A'CCwGG_T Cuts at: 383 383 Size: 6881 SfcI C'TryA_G Cuts at: 33 311 915 2035 2237 2398 2588 3258 3426 Size: 278 604 1120 202 161 190 670 168 Cuts at: 3426 3924 4609 5577 5768 33 Size: 498 685 968 191 1146 Fragments arranged by size: 1146 1120 968 685 670 604 498 278 202 191 190 168 161 SfoI GGC'GCC Cuts at: 4313 4561 4313 Size: 248 6633 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6881 SmaI CCC'GGG Cuts at: 3903 3903 Size: 6881 SmlI C'TyrA_G Cuts at: 161 889 1749 1822 3814 4036 4951 5665 5927 Size: 728 860 73 1992 222 915 714 262 Cuts at: 5927 161 Size: 1115 Fragments arranged by size: 1992 1115 915 860 728 714 262 222 73 SpeI A'CTAG_T Cuts at: 67 67 Size: 6881 SphI G_CATG'C Cuts at: 3552 3934 4434 3552 Size: 382 500 5999 Fragments arranged by size: 5999 500 382 SspI AAT'ATT Cuts at: 3009 3009 Size: 6881 StuI AGG'CCT Cuts at: 980 1885 980 Size: 905 5976 StyI C'CwwG_G Cuts at: 839 1223 1394 1880 2262 2785 4015 4369 839 Size: 384 171 486 382 523 1230 354 3351 Fragments arranged by size: 3351 1230 523 486 384 382 354 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1447 2387 3284 3291 3859 4464 4597 5056 5265 Size: 940 897 7 568 605 133 459 209 Cuts at: 5265 1447 Size: 3063 Fragments arranged by size: 3063 940 897 605 568 459 209 133 7 TatI w'GTAC_w Cuts at: 2770 6396 2770 Size: 3626 3255 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 862 894 1710 1742 2547 2579 2797 2829 6398 Size: 32 816 32 805 32 218 32 3569 Cuts at: 6398 6430 862 Size: 32 1313 Fragments arranged by size: 3569 1313 816 805 218 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 385 4678 385 Size: 4293 2588 XbaI T'CTAG_A Cuts at: 3912 3912 Size: 6881 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1036 4376 1036 Size: 3340 3541 XhoI C'TCGA_G Cuts at: 161 889 1822 3814 161 Size: 728 933 1992 3228 Fragments arranged by size: 3228 1992 933 728 XmaI C'CCGG_G Cuts at: 3901 3901 Size: 6881 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI PpiI PpuMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AloI ApaI AscI AvrII BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BspEI BssHII BstZ17I ClaI CspCI EcoRV FseI HpaI NdeI NheI NotI NruI NsiI PacI PasI PfoI PshAI PsiI PspOMI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XmnI ZraI