Restriction map for pF1KA0917
 (Linear) (Six-base) MAPSORT of: KIAA0917.seq  Check: 4721  from: 1  to: 1926



KIAA0917.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AccI GT'mk_AC

Cuts at:      0     266    1926 
   Size:        266    1660
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     875    1926 
   Size:        305     570    1051

  Fragments arranged by size: 

                1051     570     305
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1333    1926 
   Size:       1333     593
 
ApaLI G'TGCA_C

Cuts at:      0     841    1926 
   Size:        841    1085
 
ApoI r'AATT_y

Cuts at:      0      81     348     970    1926 
   Size:         81     267     622     956

  Fragments arranged by size: 

                 956     622     267      81
 
BbvCI CC'TCA_GC

Cuts at:      0    1509    1926 
   Size:       1509     417
 
BlpI GC'TnA_GC

Cuts at:      0    1139    1926 
   Size:       1139     787
 
Bme1580I G_kGCm'C

Cuts at:      0     845    1926 
   Size:        845    1081
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     893    1331    1926 
   Size:        893     438     595

  Fragments arranged by size: 

                 893     595     438
 
Bpu10I CC'TnA_GC

Cuts at:      0    1509    1926 
   Size:       1509     417
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1198    1926 
   Size:       1198     728
 
BsaBI GATnn'nnATC

Cuts at:      0     239    1926 
   Size:        239    1687
 
BsaWI w'CCGG_w

Cuts at:      0     960    1926 
   Size:        960     966
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     107     137    1657    1687    1926 
   Size:        107      30    1520      30     239

  Fragments arranged by size: 

                1520     239     107      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     161    1805    1926 
   Size:        161    1644     121

  Fragments arranged by size: 

                1644     161     121
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434    1926 
   Size:        434    1492
 
BsiHKAI G_wGCw'C

Cuts at:      0     845    1050    1926 
   Size:        845     205     876

  Fragments arranged by size: 

                 876     845     205
 
Bsp1286I G_dGCh'C

Cuts at:      0     845    1050    1926 
   Size:        845     205     876

  Fragments arranged by size: 

                 876     845     205
 
BspHI T'CATG_A

Cuts at:      0    1568    1926 
   Size:       1568     358
 
BsrDI GCAATG_nn'

Cuts at:      0    1734    1926 
   Size:       1734     192
 
BssSI C'ACGA_G

Cuts at:      0    1562    1926 
   Size:       1562     364
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     412    1926 
   Size:        412    1514
 
BstBI TT'CG_AA

Cuts at:      0     318    1926 
   Size:        318    1608
 
BstYI r'GATC_y

Cuts at:      0     240     312    1926 
   Size:        240      72    1614

  Fragments arranged by size: 

                1614     240      72
 
BstZ17I GTA'TAC

Cuts at:      0     267    1926 
   Size:        267    1659
 
BtsI GCAGTG_nn'

Cuts at:      0     671    1217    1875    1926 
   Size:        671     546     658      51

  Fragments arranged by size: 

                 671     658     546      51
 
DraI TTT'AAA

Cuts at:      0     347     866    1926 
   Size:        347     519    1060

  Fragments arranged by size: 

                1060     519     347
 
EaeI y'GGCC_r

Cuts at:      0     153     744    1517    1926 
   Size:        153     591     773     409

  Fragments arranged by size: 

                 773     591     409     153
 
EarI CTCTTCn'nnn_

Cuts at:      0     595    1316    1926 
   Size:        595     721     610

  Fragments arranged by size: 

                 721     610     595
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     875     893    1331    1926 
   Size:        305     570      18     438     595

  Fragments arranged by size: 

                 595     570     438     305      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1337    1926 
   Size:       1337     589
 
EcoO109I rG'GnC_Cy

Cuts at:      0     764    1926 
   Size:        764    1162
 
EcoRV GAT'ATC

Cuts at:      0     540    1926 
   Size:        540    1386
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     151     183    1309    1341    1657    1689    1926 
   Size:        151      32    1126      32     316      32     237

  Fragments arranged by size: 

                1126     316     237     151      32      32      32
 
HincII GTy'rAC

Cuts at:      0     582     783    1824    1926 
   Size:        582     201    1041     102

  Fragments arranged by size: 

                1041     582     201     102
 
HindIII A'AGCT_T

Cuts at:      0    1433    1926 
   Size:       1433     493
 
MscI TGG'CCA

Cuts at:      0     155     746    1926 
   Size:        155     591    1180

  Fragments arranged by size: 

                1180     591     155
 
MslI CAynn'nnrTG

Cuts at:      0     553    1117    1208    1926 
   Size:        553     564      91     718

  Fragments arranged by size: 

                 718     564     553      91
 
MspA1I CmG'CkG

Cuts at:      0     913    1153    1926 
   Size:        913     240     773

  Fragments arranged by size: 

                 913     773     240
 
PstI C_TGCA'G

Cuts at:      0     264    1870    1926 
   Size:        264    1606      56

  Fragments arranged by size: 

                1606     264      56
 
PvuII CAG'CTG

Cuts at:      0     913    1153    1926 
   Size:        913     240     773

  Fragments arranged by size: 

                 913     773     240
 
SexAI A'CCwGG_T

Cuts at:      0    1610    1926 
   Size:       1610     316
 
SfcI C'TryA_G

Cuts at:      0     260     575    1695    1866    1926 
   Size:        260     315    1120     171      60

  Fragments arranged by size: 

                1120     315     260     171      60
 
SmlI C'TyrA_G

Cuts at:      0    1177    1926 
   Size:       1177     749
 
SspI AAT'ATT

Cuts at:      0     294    1926 
   Size:        294    1632
 
StyI C'CwwG_G

Cuts at:      0     431    1926 
   Size:        431    1495
 
SwaI ATTT'AAAT

Cuts at:      0     347    1926 
   Size:        347    1579
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     601    1926 
   Size:        601    1325
 
TatI w'GTAC_w

Cuts at:      0     849    1926 
   Size:        849    1077
 
XbaI T'CTAG_A

Cuts at:      0    1314    1926 
   Size:       1314     612
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1545    1779    1926 
   Size:       1545     234     147

  Fragments arranged by size: 

                1545     234     147
 
XmnI GAAnn'nnTTC

Cuts at:      0    1019    1926 
   Size:       1019     907
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI    ApaLI     ApoI    BbvCI     BlpI Bme1580I
     BpmI   Bpu10I    BpuEI    BsaBI    BsaWI    BsaXI    BseRI     BsgI
  BsiHKAI Bsp1286I    BspHI    BsrDI    BssSI   BstAPI    BstBI    BstYI
  BstZ17I     BtsI     DraI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRV    Hin4I   HincII  HindIII     MscI     MslI   MspA1I     PstI
    PvuII    SexAI     SfcI     SmlI     SspI     StyI     SwaI    TaqII
     TatI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmrI
     BmtI     BplI     BsaI    BsaAI    BsaHI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
   BstEII    BstXI   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoRI     FalI     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     TstI  Tth111I     XhoI     XmaI     ZraI
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