Restriction map for pF1KA0904
##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AccII       AccIII      AflII       AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        AspEI       
AviII       AvrII       BamHI       BbeI        BbrPI       
BciVI       BfrI        BlnI        BsaAI       BsaBI       
BseAI       BsiWI       BsmI        BspEI       BspLU11I    
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstUI       BstZ17I     Cfr10I      CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoT22I     FseI        FspI        
KasI        KspI        MamI        MluI        MroI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PinAI       PmaCI       PmeI        PmlI        PshBI       
PvuI        RsrII       SacII       SalI        SbfI        
SexAI       SfoI        SgrAI       SnaBI       SphI        
Sse8387I    SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII        3143  GACGTC               GACGT^C
Acc65I       2699  GGTACC               G^GTACC
AccI         2942  GTCTAC               GT^[AC][GT]AC
AflIII       1984  ACACGT               A^C[GA][CT]GT
AocI         3212  CCTCAGG              CC^T[ACGT]AGG
Asp700I      1939  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2699  GGTACC               G^GTACC
AspI         3965  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcgI          324  GCACAAACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2840  TGATCA               T^GATCA
BglI         3711  GCCACAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2827  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2827  GCTCAGC              GC^T[ACGT]AGC
BsmBI         921  GAGACG               CGTCTC(1/5)
Bsp1407I     1812  TGTACA               T^GTACA
BspDI         423  ATCGAT               AT^CGAT
BspHI        2516  TCATGA               T^CATGA
BsrGI        1812  TGTACA               T^GTACA
BstBI         459  TTCGAA               TT^CGAA
Bsu36I       3212  CCTCAGG              CC^T[ACGT]AGG
CelII        2827  GCTCAGC              GC^T[ACGT]AGC
ClaI          423  ATCGAT               AT^CGAT
DraIII       4210  CACCCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         3515  CCATGG               C^C[GA][CT]GG
Eco81I       3212  CCTCAGG              CC^T[ACGT]AGG
EcoRI        2953  GAATTC               G^AATTC
FauI          959  GCGGG                CCCGC(4/6)
FbaI         2840  TGATCA               T^GATCA
HaeII        3835  AGCGCC               [GA]GCGC^[CT]
HincII       2359  GTTAAC               GT[CT]^[GA]AC
HindII       2359  GTTAAC               GT[CT]^[GA]AC
HindIII      3593  AAGCTT               A^AGCTT
HpaI         2359  GTTAAC               GTT^AAC
KpnI         2699  GGTACC               GGTAC^C
MfeI         1592  CAATTG               C^AATTG
MslI         2347  CACCGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1592  CAATTG               C^AATTG
NcoI         3515  CCATGG               C^CATGG
NspV          459  TTCGAA               TT^CGAA
PflFI        3965  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        4074  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         2516  TCATGA               T^CATGA
SacI         2020  GAGCTC               GAGCT^C
SfiI         3710  GGCCACAGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          459  TTCGAA               TT^CGAA
SmaI          356  CCCGGG               CCC^GGG
SpeI         2253  ACTAGT               A^CTAGT
SspBI        1812  TGTACA               T^GTACA
Tth111I      3965  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       4074  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2813  CCAATCTGGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         356  CCCGGG               C^CCGGG
XmaI          356  CCCGGG               C^CCGGG
XmnI         1939  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AclI         1875  AACGTT               AA^CGTT
             2732  AACGTT              
AcyI         2939  GGCGTC               G[GA]^CG[CT]C
             3143  GACGTC              
Alw44I       2485  GTGCAC               G^TGCAC
             3024  GTGCAC              
ApaI         3718  GGGCCC               GGGCC^C
             4353  GGGCCC              
ApaLI        2485  GTGCAC               G^TGCAC
             3024  GTGCAC              
BanI         2419  GGTGCC               G^G[CT][GA]CC
             2699  GGTACC              
BglII         898  AGATCT               A^GATCT
             2034  AGATCT              
BsaHI        2939  GGCGTC               G[GA]^CG[CT]C
             3143  GACGTC              
BsaWI         434  ACCGGA               [AT]^CCGG[AT]
              587  ACCGGA              
BsgI          322  CTGCAC               GTGCAG(16/14)
             4156  GTGCAG              
BsiEI         315  CGACCG               CG[GA][CT]^CG
              925  CGGTCG              
BstAPI       1219  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3544  GCACCCTCTGC         
BstEII        165  GGTGACC              G^GT[ACGT]ACC
              869  GGTCACC             
BstPI         165  GGTGACC              G^GT[ACGT]ACC
              869  GGTCACC             
BstXI         149  CCAAAGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2869  CCAGCTGTGTGG        
CfoI          490  GCGC                 GCG^C
             3836  GCGC                
EcoO65I       165  GGTGACC              G^GT[ACGT]ACC
              869  GGTCACC             
EcoRV         223  GATATC               GAT^ATC
              438  GATATC              
HgaI          102  GCGTC                GACGC(5/10)
             2940  GCGTC               
HhaI          490  GCGC                 GCG^C
             3836  GCGC                
Hin1I        2939  GGCGTC               G[GA]^CG[CT]C
             3143  GACGTC              
HinP1I        490  GCGC                 G^CGC
             3836  GCGC                
PaeR7I        765  CTCGAG               C^TCGAG
              807  CTCGAG              
Psp1406I     1875  AACGTT               AA^CGTT
             2732  AACGTT              
SapI         3172  GAAGAGC              GCTCTTC(1/4)
             1675  GCTCTTC             
XhoI          765  CTCGAG               C^TCGAG
              807  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaI        AvaII       
BalI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PshAI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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