Restriction map for pF1KA0872
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        AocI        Aor51HI     AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaI        BsaWI       BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRI       
EcoRV       FbaI        FseI        FspI        HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MseI        MunI        NdeI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SbfI        SexAI       SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tth111I     XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI          845  GTCGAC               GT^[AC][GT]AC
AcsI         1453  AAATTC               [GA]^AATT[CT]
ApaI          504  GGGCCC               GGGCC^C
ApoI         1453  AAATTC               [GA]^AATT[CT]
AvaI           34  CCCGGG               C^[CT]CG[GA]G
BalI          521  TGGCCA               TGG^CCA
BbsI          124  GAAGAC               GAAGAC(2/6)
BpuAI         124  GAAGAC               GAAGAC(2/6)
BsiEI         843  CGGTCG               CG[GA][CT]^CG
BsoBI          34  CCCGGG               C^[CT]CG[GA]G
BspLU11I      256  ACATGT               A^CATGT
BssSI         517  CTCGTG               CACGAG(-5/-1)
DraIII        779  CACTAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          534  GACTTCTCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1555  CTCTTC               CTCTTC(1/4)
EcoT22I      1120  ATGCAT               ATGCA^T
HgaI          775  GACGC                GACGC(5/10)
HincII        845  GTCGAC               GT[CT]^[GA]AC
HindII        845  GTCGAC               GT[CT]^[GA]AC
Ksp632I      1555  CTCTTC               CTCTTC(1/4)
MluNI         521  TGGCCA               TGG^CCA
MscI          521  TGGCCA               TGG^CCA
MslI          968  CACCTGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI          570  GCTAGC               G^CTAGC
NsiI         1120  ATGCAT               ATGCA^T
SacI         1459  GAGCTC               GAGCT^C
SalI          845  GTCGAC               G^TCGAC
SapI         1554  GCTCTTC              GCTCTTC(1/4)
ScaI         1577  AGTACT               AGT^ACT
SmaI           34  CCCGGG               CCC^GGG
Tsp509I      1454  AATT                 ^AATT
XmaCI          34  CCCGGG               C^CCGGG
XmaI           34  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   41 enzymes) #
###################################

AcyI         1100  GGCGCC               G[GA]^CG[CT]C
             1218  GGCGCC              
AfaI         1353  GTAC                 GT^AC
             1578  GTAC                
AflIII        256  ACATGT               A^C[GA][CT]GT
              604  ACACGT              
Alw44I        577  GTGCAC               G^TGCAC
              935  GTGCAC              
ApaLI         577  GTGCAC               G^TGCAC
              935  GTGCAC              
BamHI         117  GGATCC               G^GATCC
              148  GGATCC              
BbeI         1100  GGCGCC               GGCGC^C
             1218  GGCGCC              
BbrPI         605  CACGTG               CAC^GTG
             1411  CACGTG              
BbvCI        1473  CCTCAGC              CCTCAGC(-5/-2)
              472  GCTGAGG             
BglI          943  GCCTTTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1012  GCCTACGTGGC         
BsaHI        1100  GGCGCC               G[GA]^CG[CT]C
             1218  GGCGCC              
BsgI          436  GTGCAG               GTGCAG(16/14)
             1204  GTGCAG              
BsmBI        1372  CGTCTC               CGTCTC(1/5)
              773  GAGACG              
BsrBI         868  GAGCGG               CCGCTC(-3/-3)
             1330  GAGCGG              
BstAPI        778  GCACTAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              912  GCAGTTGCTGC         
BstEII       1443  GGTGACC              G^GT[ACGT]ACC
             1485  GGTGACC             
BstPI        1443  GGTGACC              G^GT[ACGT]ACC
             1485  GGTGACC             
BstYI         117  GGATCC               [GA]^GATC[CT]
              148  GGATCC              
DsaI          404  CCATGG               C^C[GA][CT]GG
             1085  CCATGG              
EcoNI         495  CCTACAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1146  CCTTGGGGAGG         
EcoO65I      1443  GGTGACC              G^GT[ACGT]ACC
             1485  GGTGACC             
Hin1I        1100  GGCGCC               G[GA]^CG[CT]C
             1218  GGCGCC              
HinfI         228  GAGTC                G^A[ACGT]TC
             1637  GACTC               
KasI         1100  GGCGCC               G^GCGCC
             1218  GGCGCC              
MflI          117  GGATCC               [GA]^GATC[CT]
              148  GGATCC              
NaeI          890  GCCGGC               GCC^GGC
             1097  GCCGGC              
NarI         1100  GGCGCC               GG^CGCC
             1218  GGCGCC              
NcoI          404  CCATGG               C^CATGG
             1085  CCATGG              
NgoMIV        890  GCCGGC               G^CCGGC
             1097  GCCGGC              
PflMI         480  CCACAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1080  CCATGCCATGG         
PleI         1637  GACTC                GAGTC(4/5)
              228  GAGTC               
PmaCI         605  CACGTG               CAC^GTG
             1411  CACGTG              
PmlI          605  CACGTG               CAC^GTG
             1411  CACGTG              
PpuMI         293  GGGACCC              [GA]G^G[AT]CC[CT]
              713  AGGACCC             
PstI          233  CTGCAG               CTGCA^G
              910  CTGCAG              
PvuII         553  CAGCTG               CAG^CTG
              902  CAGCTG              
RsaI         1353  GTAC                 GT^AC
             1578  GTAC                
SfoI         1100  GGCGCC               GGC^GCC
             1218  GGCGCC              
SphI         1122  GCATGC               GCATG^C
             1365  GCATGC              
Van91I        480  CCACAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1080  CCATGCCATGG         
XhoII         117  GGATCC               [GA]^GATC[CT]
              148  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
AspHI       AvaII       BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     
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