Restriction map for pF1KA0872
 (Linear) (Six-base) MAPSORT of: KIAA0872.seq  Check: 7111  from: 1  to: 1641



KIAA0872.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1486    1641 
   Size:       1486     155
 
AccI GT'mk_AC

Cuts at:      0     846    1641 
   Size:        846     795
 
AflIII A'CryG_T

Cuts at:      0     256     604    1641 
   Size:        256     348    1037

  Fragments arranged by size: 

                1037     348     256
 
AleI CACnn'nnGTG

Cuts at:      0     972    1641 
   Size:        972     669
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     990    1641 
   Size:        990     651
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      49      81    1641 
   Size:         49      32    1560

  Fragments arranged by size: 

                1560      49      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     241     811     918    1341    1641 
   Size:        241     570     107     423     300

  Fragments arranged by size: 

                 570     423     300     241     107
 
ApaI G_GGCC'C

Cuts at:      0     508    1641 
   Size:        508    1133
 
ApaLI G'TGCA_C

Cuts at:      0     577     935    1641 
   Size:        577     358     706

  Fragments arranged by size: 

                 706     577     358
 
ApoI r'AATT_y

Cuts at:      0    1453    1641 
   Size:       1453     188
 
AvaI C'yCGr_G

Cuts at:      0      34    1641 
   Size:         34    1607
 
BamHI G'GATC_C

Cuts at:      0     117     148    1641 
   Size:        117      31    1493

  Fragments arranged by size: 

                1493     117      31
 
BanI G'GyrC_C

Cuts at:      0     736     966    1060    1100    1132    1218    1641 
   Size:        736     230      94      40      32      86     423

  Fragments arranged by size: 

                 736     423     230      94      86      40      32
 
BanII G_rGCy'C

Cuts at:      0     508     726    1463    1641 
   Size:        508     218     737     178

  Fragments arranged by size: 

                 737     508     218     178
 
BbeI G_GCGC'C

Cuts at:      0    1104    1222    1641 
   Size:       1104     118     419

  Fragments arranged by size: 

                1104     419     118
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     131    1641 
   Size:        131    1510
 
BbvCI CC'TCA_GC

Cuts at:      0     473    1474    1641 
   Size:        473    1001     167

  Fragments arranged by size: 

                1001     473     167
 
BfrBI ATG'CAT

Cuts at:      0    1122    1641 
   Size:       1122     519
 
BglI GCCn_nnn'nGGC

Cuts at:      0     949    1018    1641 
   Size:        949      69     623

  Fragments arranged by size: 

                 949     623      69
 
Bme1580I G_kGCm'C

Cuts at:      0     508     581     755     939     969    1146    1641 
   Size:        508      73     174     184      30     177     495

  Fragments arranged by size: 

                 508     495     184     177     174      73      30
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      65     186     293     936    1278    1641 
   Size:         65     121     107     643     342     363

  Fragments arranged by size: 

                 643     363     342     121     107      65
 
BmtI G_CTAG'C

Cuts at:      0     574    1641 
   Size:        574    1067
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     291     745    1641 
   Size:        291     454     896

  Fragments arranged by size: 

                 896     454     291
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     246     299    1641 
   Size:        157      89      53    1342

  Fragments arranged by size: 

                1342     157      89      53
 
Bpu10I CC'TnA_GC

Cuts at:      0     473    1298    1474    1641 
   Size:        473     825     176     167

  Fragments arranged by size: 

                 825     473     176     167
 
BsaAI yAC'GTr

Cuts at:      0     607    1017    1413    1641 
   Size:        607     410     396     228

  Fragments arranged by size: 

                 607     410     396     228
 
BsaHI Gr'CG_yC

Cuts at:      0    1101    1219    1641 
   Size:       1101     118     422

  Fragments arranged by size: 

                1101     422     118
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     379     409    1369    1399    1641 
   Size:        379      30     960      30     242

  Fragments arranged by size: 

                 960     379     242      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      26     322     781    1339    1342    1558    1641 
   Size:         26     296     459     558       3     216      83

  Fragments arranged by size: 

                 558     459     296     216      83      26       3
 
BseYI C'CCAG_C

Cuts at:      0     172     551     900    1529    1641 
   Size:        172     379     349     629     112

  Fragments arranged by size: 

                 629     379     349     172     112
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     457    1225    1641 
   Size:        457     768     416

  Fragments arranged by size: 

                 768     457     416
 
BsiEI CG_ry'CG

Cuts at:      0     846    1641 
   Size:        846     795
 
BsiHKAI G_wGCw'C

Cuts at:      0     581     598     939    1191    1463    1641 
   Size:        581      17     341     252     272     178

  Fragments arranged by size: 

                 581     341     272     252     178      17
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     767    1378    1641 
   Size:        767     611     263

  Fragments arranged by size: 

                 767     611     263
 
Bsp1286I G_dGCh'C

Cuts at:      0     508     581     598     726     755     939     969    1146 
   Size:        508      73      17     128      29     184      30     177

Cuts at:   1146    1191    1463    1641 
   Size:         45     272     178

  Fragments arranged by size: 

                 508     272     184     178     177     128      73      45
                  30      29      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     917    1477    1486    1641 
   Size:        917     560       9     155

  Fragments arranged by size: 

                 917     560     155       9
 
BsrBI CCG'CTC

Cuts at:      0     870    1332    1641 
   Size:        870     462     309

  Fragments arranged by size: 

                 870     462     309
 
BsrFI r'CCGG_y

Cuts at:      0     890    1097    1550    1641 
   Size:        890     207     453      91

  Fragments arranged by size: 

                 890     453     207      91
 
BssSI C'ACGA_G

Cuts at:      0     517    1641 
   Size:        517    1124
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     784     918    1641 
   Size:        784     134     723

  Fragments arranged by size: 

                 784     723     134
 
BstEII G'GTnAC_C

Cuts at:      0    1443    1485    1641 
   Size:       1443      42     156

  Fragments arranged by size: 

                1443     156      42
 
BstYI r'GATC_y

Cuts at:      0     117     148    1641 
   Size:        117      31    1493

  Fragments arranged by size: 

                1493     117      31
 
BtgI C'CryG_G

Cuts at:      0     404    1085    1641 
   Size:        404     681     556

  Fragments arranged by size: 

                 681     556     404
 
BtsI GCAGTG_nn'

Cuts at:      0      17    1641 
   Size:         17    1624
 
DraIII CAC_nnn'GTG

Cuts at:      0     784    1641 
   Size:        784     857
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     540    1641 
   Size:        540    1101
 
EaeI y'GGCC_r

Cuts at:      0     521     881    1088    1641 
   Size:        521     360     207     553

  Fragments arranged by size: 

                 553     521     360     207
 
EarI CTCTTCn'nnn_

Cuts at:      0    1561    1641 
   Size:       1561      80
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     643    1641 
   Size:        643     998
 
EcoICRI GAG'CTC

Cuts at:      0    1461    1641 
   Size:       1461     180
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     246     299    1641 
   Size:        157      89      53    1342

  Fragments arranged by size: 

                1342     157      89      53
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     499    1150    1641 
   Size:        499     651     491

  Fragments arranged by size: 

                 651     499     491
 
EcoO109I rG'GnC_Cy

Cuts at:      0     132     201     294     504     714    1308    1641 
   Size:        132      69      93     210     210     594     333

  Fragments arranged by size: 

                 594     333     210     210     132      93      69
 
HaeII r_GCGC'y

Cuts at:      0     394     811     823    1104    1222    1641 
   Size:        394     417      12     281     118     419

  Fragments arranged by size: 

                 419     417     394     281     118      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     190     222     379     411     440     472    1641 
   Size:        190      32     157      32      29      32    1169

  Fragments arranged by size: 

                1169     190     157      32      32      32      29
 
HincII GTy'rAC

Cuts at:      0     847    1641 
   Size:        847     794
 
KasI G'GCGC_C

Cuts at:      0    1100    1218    1641 
   Size:       1100     118     423

  Fragments arranged by size: 

                1100     423     118
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     819    1325    1641 
   Size:        819     506     316

  Fragments arranged by size: 

                 819     506     316
 
MscI TGG'CCA

Cuts at:      0     523    1641 
   Size:        523    1118
 
MslI CAynn'nnrTG

Cuts at:      0     972    1641 
   Size:        972     669
 
MspA1I CmG'CkG

Cuts at:      0     324     555     768     904    1251    1338    1641 
   Size:        324     231     213     136     347      87     303

  Fragments arranged by size: 

                 347     324     303     231     213     136      87
 
NaeI GCC'GGC

Cuts at:      0     892    1099    1641 
   Size:        892     207     542

  Fragments arranged by size: 

                 892     542     207
 
NarI GG'CG_CC

Cuts at:      0    1101    1219    1641 
   Size:       1101     118     422

  Fragments arranged by size: 

                1101     422     118
 
NcoI C'CATG_G

Cuts at:      0     404    1085    1641 
   Size:        404     681     556

  Fragments arranged by size: 

                 681     556     404
 
NgoMIV G'CCGG_C

Cuts at:      0     890    1097    1641 
   Size:        890     207     544

  Fragments arranged by size: 

                 890     544     207
 
NheI G'CTAG_C

Cuts at:      0     570    1641 
   Size:        570    1071
 
NsiI A_TGCA'T

Cuts at:      0    1124    1641 
   Size:       1124     517
 
NspI r_CATG'y

Cuts at:      0     260     334    1126    1369    1641 
   Size:        260      74     792     243     272

  Fragments arranged by size: 

                 792     272     260     243      74
 
PciI A'CATG_T

Cuts at:      0     256    1641 
   Size:        256    1385
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     486    1086    1641 
   Size:        486     600     555

  Fragments arranged by size: 

                 600     555     486
 
PmlI CAC'GTG

Cuts at:      0     607    1413    1641 
   Size:        607     806     228

  Fragments arranged by size: 

                 806     607     228
 
PpuMI rG'GwC_Cy

Cuts at:      0     294     714    1641 
   Size:        294     420     927

  Fragments arranged by size: 

                 927     420     294
 
PsiI TTA'TAA

Cuts at:      0     853    1641 
   Size:        853     788
 
PspOMI G'GGCC_C

Cuts at:      0     504    1641 
   Size:        504    1137
 
PstI C_TGCA'G

Cuts at:      0     237     914    1641 
   Size:        237     677     727

  Fragments arranged by size: 

                 727     677     237
 
PvuII CAG'CTG

Cuts at:      0     555     904    1641 
   Size:        555     349     737

  Fragments arranged by size: 

                 737     555     349
 
SacI G_AGCT'C

Cuts at:      0    1463    1641 
   Size:       1463     178
 
SalI G'TCGA_C

Cuts at:      0     845    1641 
   Size:        845     796
 
SanDI GG'GwC_CC

Cuts at:      0     294    1641 
   Size:        294    1347
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1561    1641 
   Size:       1561      80
 
ScaI AGT'ACT

Cuts at:      0    1579    1641 
   Size:       1579      62
 
SfcI C'TryA_G

Cuts at:      0     233     910    1197    1314    1641 
   Size:        233     677     287     117     327

  Fragments arranged by size: 

                 677     327     287     233     117
 
SfoI GGC'GCC

Cuts at:      0    1102    1220    1641 
   Size:       1102     118     421

  Fragments arranged by size: 

                1102     421     118
 
SmaI CCC'GGG

Cuts at:      0      36    1641 
   Size:         36    1605
 
SphI G_CATG'C

Cuts at:      0    1126    1369    1641 
   Size:       1126     243     272

  Fragments arranged by size: 

                1126     272     243
 
StyI C'CwwG_G

Cuts at:      0     152     404    1085    1128    1146    1490    1641 
   Size:        152     252     681      43      18     344     151

  Fragments arranged by size: 

                 681     344     252     152     151      43      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     186     397     704     724     918     922    1078    1431 
   Size:        186     211     307      20     194       4     156     353

Cuts at:   1431    1521    1587    1641 
   Size:         90      66      54

  Fragments arranged by size: 

                 353     307     211     194     186     156      90      66
                  54      20       4
 
TatI w'GTAC_w

Cuts at:      0    1577    1641 
   Size:       1577      64
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1369    1401    1641 
   Size:       1369      32     240

  Fragments arranged by size: 

                1369     240      32
 
XmaI C'CCGG_G

Cuts at:      0      34    1641 
   Size:         34    1607
 

 Enzymes that do cut:

     AarI     AccI   AflIII     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI    BfrBI     BglI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI   BstAPI
   BstEII    BstYI     BtgI     BtsI   DraIII     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NsiI     NspI     PciI    PflMI     PmlI    PpuMI
     PsiI   PspOMI     PstI    PvuII     SacI     SalI    SanDI     SapI
     ScaI     SfcI     SfoI     SmaI     SphI     StyI    TaqII     TatI
     TstI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AscI     AseI    AsiSI    AvrII     BaeI     BcgI    BciVI     BclI
    BglII     BlpI    BmgBI    BpuEI     BsaI    BsaBI    BsaWI    BsiWI
     BsmI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BstBI    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     EagI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     NdeI     NotI     NruI     PacI     PasI     PfoI
     PmeI     PpiI    PshAI    PspXI     PsrI     PvuI    RsrII    SacII
     SbfI    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI
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