Restriction map for pF1KA0840
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# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AlwI        AocI        
ApaI        ApoI        AscI        AseI        AsnI        
AspI        AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BclI        BfaI        BfrI        
BlnI        BsaHI       BsaWI       BseAI       BsiWI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BssSI       Bst1107I    BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        EarI        
Eco81I      EcoNI       EcoRI       EcoT22I     FbaI        
FseI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        Ksp632I     MaeI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SapI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        SwaI        Tsp509I     Tth111I     XbaI        


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# ONE CUT ENZYMES (   64 enzymes) #
###################################

Acc65I       1058  GGTACC               G^GTACC
AflIII        548  ACGTGT               A^C[GA][CT]GT
AhdI          647  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        262  GTGCAC               G^TGCAC
Aor51HI      1436  AGCGCT               AGC^GCT
ApaLI         262  GTGCAC               G^TGCAC
Asp700I      1275  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1058  GGTACC               G^GTACC
AspEI         647  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         143  TGCGCA               TGC^GCA
BanI         1058  GGTACC               G^G[CT][GA]CC
BanII         213  GGGCTC               G[GA]GC[CT]^C
BbsI          124  GAAGAC               GAAGAC(2/6)
BciVI        1243  GTATCC               GTATCC(6/5)
BglI          473  GCCTGACGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         371  AGATCT               A^GATCT
BpuAI         124  GAAGAC               GAAGAC(2/6)
BsaAI        1108  TACGTG               [CT]AC^GT[GA]
BsaBI         813  GATTTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         225  CTCCTC               GAGGAG(10/8)
BsiEI        1080  CGGCCG               CG[GA][CT]^CG
BsmBI         247  GAGACG               CGTCTC(1/5)
Bsp1407I      611  TGTACA               T^GTACA
BsrBI         295  CCGCTC               CCGCTC(-3/-3)
BsrGI         611  TGTACA               T^GTACA
BssHII        410  GCGCGC               G^CGCGC
BstEII       1086  GGTCACC              G^GT[ACGT]ACC
BstPI        1086  GGTCACC              G^GT[ACGT]ACC
BstXI         488  CCATCAACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         371  AGATCT               [GA]^GATC[CT]
DraIII       1174  CACGGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1387  GACTGCGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1080  CGGCCG               C^GGCCG
Eam1105I      647  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1080  CGGCCG               C^GGCCG
Eco47III     1436  AGCGCT               AGC^GCT
Eco52I       1080  CGGCCG               C^GGCCG
EcoO65I      1086  GGTCACC              G^GT[ACGT]ACC
EcoRV        1222  GATATC               GAT^ATC
FspI          143  TGCGCA               TGC^GCA
HaeII        1436  AGCGCT               [GA]GCGC^[CT]
KpnI         1058  GGTACC               GGTAC^C
KspI          451  CCGCGG               CCGC^GG
MamI          813  GATTTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          371  AGATCT               [GA]^GATC[CT]
MseI          785  TTAA                 T^TAA
NspI          140  GCATGC               [GA]CATG^[CT]
PaeR7I         48  CTCGAG               C^TCGAG
PpuMI         446  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII         397  CAGCTG               CAG^CTG
SacII         451  CCGCGG               CCGC^GG
ScaI         1118  AGTACT               AGT^ACT
SexAI         953  ACCTGGT              A^CC[AT]GGT
SmaI          770  CCCGGG               CCC^GGG
SphI          140  GCATGC               GCATG^C
SspBI         611  TGTACA               T^GTACA
TfiI          186  GAATC                G^A[AT]TC
Tru9I         785  TTAA                 T^TAA
XcmI          818  CCATCCGCTACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI           48  CTCGAG               C^TCGAG
XhoII         371  AGATCT               [GA]^GATC[CT]
XmaCI         770  CCCGGG               C^CCGGG
XmaI          770  CCCGGG               C^CCGGG
XmnI         1275  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AvaI           48  CTCGAG               C^[CT]CG[GA]G
              770  CCCGGG              
BalI          254  TGGCCA               TGG^CCA
             1112  TGGCCA              
BlpI          984  GCTGAGC              GC^T[ACGT]AGC
             1296  GCTCAGC             
Bpu1102I      984  GCTGAGC              GC^T[ACGT]AGC
             1296  GCTCAGC             
BsaI          484  GAGACC               GGTCTC(1/5)
             1395  GGTCTC              
BsoBI          48  CTCGAG               C^[CT]CG[GA]G
              770  CCCGGG              
BspMI         754  ACCTGC               ACCTGC(4/8)
              911  ACCTGC              
BsrFI         344  ACCGGC               [GA]^CCGG[CT]
             1324  ACCGGC              
BsrI          648  ACTGG                ACTGG(1/-1)
              624  CCAGT               
BstAPI        524  GCAGACTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1433  GCAAGCGCTGC         
CelII         984  GCTGAGC              GC^T[ACGT]AGC
             1296  GCTCAGC             
Cfr10I        344  ACCGGC               [GA]^CCGG[CT]
             1324  ACCGGC              
DraII         446  GGGACCC              [GA]G^G[ACGT]CC[CT]
             1406  AGGCCCT             
EcoO109I      446  GGGACCC              [GA]G^G[ACGT]CC[CT]
             1406  AGGCCCT             
EcoT14I       257  CCATGG               C^C[AT][AT]GG
              362  CCATGG              
MluNI         254  TGGCCA               TGG^CCA
             1112  TGGCCA              
MscI          254  TGGCCA               TGG^CCA
             1112  TGGCCA              
NcoI          257  CCATGG               C^CATGG
              362  CCATGG              
PflMI         357  CCACTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1173  CCACGGTGTGG         
PstI          585  CTGCAG               CTGCA^G
             1122  CTGCAG              
SfcI          585  CTGCAG               C^T[GA][CT]AG
             1122  CTGCAG              
StyI          257  CCATGG               C^C[AT][AT]GG
              362  CCATGG              
TaqI           49  TCGA                 T^CGA
             1448  TCGA                
TthHB8I        49  TCGA                 T^CGA
             1448  TCGA                
Van91I        357  CCACTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1173  CCACGGTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StuI        TseI        Tsp45I      TspRI       
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