Restriction map for pF1KA0840
 (Linear) (Six-base) MAPSORT of: KIAA0840.seq  Check: 770  from: 1  to: 1473



KIAA0840.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

Acc65I G'GTAC_C

Cuts at:      0    1058    1473 
   Size:       1058     415
 
AfeI AGC'GCT

Cuts at:      0    1438    1473 
   Size:       1438      35
 
AflIII A'CryG_T

Cuts at:      0     548    1473 
   Size:        548     925
 
AhdI GACnn_n'nnGTC

Cuts at:      0     652    1473 
   Size:        652     821
 
AleI CACnn'nnGTG

Cuts at:      0    1093    1473 
   Size:       1093     380
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1147    1473 
   Size:       1147     326
 
AlwNI CAG_nnn'CTG

Cuts at:      0     530     584     998    1374    1473 
   Size:        530      54     414     376      99

  Fragments arranged by size: 

                 530     414     376      99      54
 
ApaLI G'TGCA_C

Cuts at:      0     262    1473 
   Size:        262    1211
 
AvaI C'yCGr_G

Cuts at:      0      48     770    1473 
   Size:         48     722     703

  Fragments arranged by size: 

                 722     703      48
 
BanI G'GyrC_C

Cuts at:      0    1058    1473 
   Size:       1058     415
 
BanII G_rGCy'C

Cuts at:      0     217    1473 
   Size:        217    1256
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     131    1473 
   Size:        131    1342
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1254    1473 
   Size:       1254     219
 
BglI GCCn_nnn'nGGC

Cuts at:      0     479    1473 
   Size:        479     994
 
BglII A'GATC_T

Cuts at:      0     371    1473 
   Size:        371    1102
 
BlpI GC'TnA_GC

Cuts at:      0     985    1297    1473 
   Size:        985     312     176

  Fragments arranged by size: 

                 985     312     176
 
Bme1580I G_kGCm'C

Cuts at:      0     266    1473 
   Size:        266    1207
 
BmgBI CAC'GTC

Cuts at:      0      66    1095    1473 
   Size:         66    1029     378

  Fragments arranged by size: 

                1029     378      66
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     618    1473 
   Size:        618     855
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     480     739     874    1063    1279    1351    1473 
   Size:        297     183     259     135     189     216      72     122

  Fragments arranged by size: 

                 297     259     216     189     183     135     122      72
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     136    1063    1473 
   Size:        136     927     410

  Fragments arranged by size: 

                 927     410     136
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493    1273    1288    1473 
   Size:        493     780      15     185

  Fragments arranged by size: 

                 780     493     185      15
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     478    1401    1473 
   Size:        478     923      72

  Fragments arranged by size: 

                 923     478      72
 
BsaAI yAC'GTr

Cuts at:      0    1110    1473 
   Size:       1110     363
 
BsaBI GATnn'nnATC

Cuts at:      0     817    1473 
   Size:        817     656
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     173     203     658     688     955     985    1473 
   Size:        173      30     455      30     267      30     488

  Fragments arranged by size: 

                 488     455     267     173      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     216    1473 
   Size:        216    1257
 
BseYI C'CCAG_C

Cuts at:      0     160     165    1473 
   Size:        160       5    1308

  Fragments arranged by size: 

                1308     160       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     388     853     873    1185    1473 
   Size:        388     465      20     312     288

  Fragments arranged by size: 

                 465     388     312     288      20
 
BsiEI CG_ry'CG

Cuts at:      0    1083    1473 
   Size:       1083     390
 
BsiHKAI G_wGCw'C

Cuts at:      0     157     266     725    1454    1473 
   Size:        157     109     459     729      19

  Fragments arranged by size: 

                 729     459     157     109      19
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     241    1473 
   Size:        241    1232
 
Bsp1286I G_dGCh'C

Cuts at:      0     157     217     266     725    1454    1473 
   Size:        157      60      49     459     729      19

  Fragments arranged by size: 

                 729     459     157      60      49      19
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     763     920    1473 
   Size:        763     157     553

  Fragments arranged by size: 

                 763     553     157
 
BsrBI CCG'CTC

Cuts at:      0     297    1473 
   Size:        297    1176
 
BsrFI r'CCGG_y

Cuts at:      0     344    1324    1473 
   Size:        344     980     149

  Fragments arranged by size: 

                 980     344     149
 
BsrGI T'GTAC_A

Cuts at:      0     611    1473 
   Size:        611     862
 
BssHII G'CGCG_C

Cuts at:      0     410    1473 
   Size:        410    1063
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     530    1439    1473 
   Size:        530     909      34

  Fragments arranged by size: 

                 909     530      34
 
BstEII G'GTnAC_C

Cuts at:      0    1086    1473 
   Size:       1086     387
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     495    1473 
   Size:        495     978
 
BstYI r'GATC_y

Cuts at:      0     371    1473 
   Size:        371    1102
 
BtgI C'CryG_G

Cuts at:      0     257     362     451    1173    1400    1473 
   Size:        257     105      89     722     227      73

  Fragments arranged by size: 

                 722     257     227     105      89      73
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     603     861    1053    1473 
   Size:        603     258     192     420

  Fragments arranged by size: 

                 603     420     258     192
 
BtsI GCAGTG_nn'

Cuts at:      0      36     885    1074    1428    1473 
   Size:         36     849     189     354      45

  Fragments arranged by size: 

                 849     354     189      45      36
 
DraIII CAC_nnn'GTG

Cuts at:      0    1179    1473 
   Size:       1179     294
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1393    1473 
   Size:       1393      80
 
EaeI y'GGCC_r

Cuts at:      0     254    1080    1112    1326    1346    1473 
   Size:        254     826      32     214      20     127

  Fragments arranged by size: 

                 826     254     214     127      32      20
 
EagI C'GGCC_G

Cuts at:      0    1080    1473 
   Size:       1080     393
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     460     916    1473 
   Size:        460     456     557

  Fragments arranged by size: 

                 557     460     456
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     480     739     874    1063    1279    1351    1473 
   Size:        297     183     259     135     189     216      72     122

  Fragments arranged by size: 

                 297     259     216     189     183     135     122      72
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     447    1407    1473 
   Size:        447     960      66

  Fragments arranged by size: 

                 960     447      66
 
EcoRV GAT'ATC

Cuts at:      0    1224    1473 
   Size:       1224     249
 
FspI TGC'GCA

Cuts at:      0     145    1473 
   Size:        145    1328
 
FspAI rTGC'GCAy

Cuts at:      0     145    1473 
   Size:        145    1328
 
HaeII r_GCGC'y

Cuts at:      0    1440    1473 
   Size:       1440      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     638     670     685     717    1473 
   Size:        638      32      15      32     756

  Fragments arranged by size: 

                 756     638      32      32      15
 
KpnI G_GTAC'C

Cuts at:      0    1062    1473 
   Size:       1062     411
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     701    1271    1473 
   Size:        155     546     570     202

  Fragments arranged by size: 

                 570     546     202     155
 
MscI TGG'CCA

Cuts at:      0     256    1114    1473 
   Size:        256     858     359

  Fragments arranged by size: 

                 858     359     256
 
MslI CAynn'nnrTG

Cuts at:      0     493     728    1093    1473 
   Size:        493     235     365     380

  Fragments arranged by size: 

                 493     380     365     235
 
MspA1I CmG'CkG

Cuts at:      0     399     407     428     453     920    1473 
   Size:        399       8      21      25     467     553

  Fragments arranged by size: 

                 553     467     399      25      21       8
 
NcoI C'CATG_G

Cuts at:      0     257     362    1473 
   Size:        257     105    1111

  Fragments arranged by size: 

                1111     257     105
 
NspI r_CATG'y

Cuts at:      0     144    1473 
   Size:        144    1329
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     363    1179    1473 
   Size:        363     816     294

  Fragments arranged by size: 

                 816     363     294
 
PfoI T'CCnGG_A

Cuts at:      0     350    1382    1473 
   Size:        350    1032      91

  Fragments arranged by size: 

                1032     350      91
 
PpuMI rG'GwC_Cy

Cuts at:      0     447    1473 
   Size:        447    1026
 
PspXI vC'TCGA_Gb

Cuts at:      0      48    1473 
   Size:         48    1425
 
PstI C_TGCA'G

Cuts at:      0     589    1126    1473 
   Size:        589     537     347

  Fragments arranged by size: 

                 589     537     347
 
PvuII CAG'CTG

Cuts at:      0     399    1473 
   Size:        399    1074
 
SacII CC_GC'GG

Cuts at:      0     454    1473 
   Size:        454    1019
 
SanDI GG'GwC_CC

Cuts at:      0     447    1473 
   Size:        447    1026
 
ScaI AGT'ACT

Cuts at:      0    1120    1473 
   Size:       1120     353
 
SexAI A'CCwGG_T

Cuts at:      0     953    1473 
   Size:        953     520
 
SfcI C'TryA_G

Cuts at:      0     585    1122    1473 
   Size:        585     537     351

  Fragments arranged by size: 

                 585     537     351
 
SmaI CCC'GGG

Cuts at:      0     772    1473 
   Size:        772     701
 
SmlI C'TyrA_G

Cuts at:      0      48     508    1288    1303    1473 
   Size:         48     460     780      15     170

  Fragments arranged by size: 

                 780     460     170      48      15
 
SphI G_CATG'C

Cuts at:      0     144    1473 
   Size:        144    1329
 
StuI AGG'CCT

Cuts at:      0     778     866     944    1473 
   Size:        778      88      78     529

  Fragments arranged by size: 

                 778     529      88      78
 
StyI C'CwwG_G

Cuts at:      0     257     362    1473 
   Size:        257     105    1111

  Fragments arranged by size: 

                1111     257     105
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     617     916     949    1473 
   Size:        617     299      33     524

  Fragments arranged by size: 

                 617     524     299      33
 
TatI w'GTAC_w

Cuts at:      0      76     611    1118    1473 
   Size:         76     535     507     355

  Fragments arranged by size: 

                 535     507     355      76
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     173     205    1473 
   Size:        173      32    1268

  Fragments arranged by size: 

                1268     173      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     825    1473 
   Size:        825     648
 
XhoI C'TCGA_G

Cuts at:      0      48    1473 
   Size:         48    1425
 
XmaI C'CCGG_G

Cuts at:      0     770    1473 
   Size:        770     703
 
XmnI GAAnn'nnTTC

Cuts at:      0    1279    1473 
   Size:       1279     194
 

 Enzymes that do cut:

   Acc65I     AfeI   AflIII     AhdI     AleI     AlfI    AlwNI    ApaLI
     AvaI     BanI    BanII     BbsI    BciVI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI
     EagI     EciI  Eco57MI EcoO109I    EcoRV     FspI    FspAI    HaeII
    Hin4I     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NspI
    PflMI     PfoI    PpuMI    PspXI     PstI    PvuII    SacII    SanDI
     ScaI    SexAI     SfcI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI    AflII     AgeI     AloI
     ApaI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI     BclI    BfrBI     BmtI     BplI    BsaHI
    BsaWI    BsiWI     BsmI    BspEI    BspHI    BsrDI    BssSI    BstBI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI     EarI  EcoICRI    EcoNI
    EcoRI     FalI     FseI   HincII  HindIII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI     PsrI     PvuI    RsrII     SacI     SalI     SapI
     SbfI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI  Tth111I     XbaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com