Restriction map for
pF1KA0810
(Circular) (Six-base) MAPSORT of: pF1KA0810.seq Check: 6368 from: 1 to: 5549
pF1KA0810 5549 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1281 1281
Size: 5549
AccI GT'mk_AC
Cuts at: 2500 2587 2500
Size: 87 5462
Acc65I G'GTAC_C
Cuts at: 989 2216 2565 989
Size: 1227 349 3973
Fragments arranged by size:
3973 1227 349
AclI AA'CG_TT
Cuts at: 5389 5389
Size: 5549
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 376 1246 3374 3806 4159 4821 182
Size: 194 870 2128 432 353 662 910
Fragments arranged by size:
2128 910 870 662 432 353 194
AfeI AGC'GCT
Cuts at: 345 345
Size: 5549
AflIII A'CryG_T
Cuts at: 128 170 425 1199 1668 2901 4701 4982 128
Size: 42 255 774 469 1233 1800 281 695
Fragments arranged by size:
1800 1233 774 695 469 281 255 42
AleI CACnn'nnGTG
Cuts at: 1325 1325
Size: 5549
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 712 1991 712
Size: 1279 4270
AlwNI CAG_nnn'CTG
Cuts at: 668 867 958 1236 1365 1602 1788 1863 2373
Size: 199 91 278 129 237 186 75 510
Cuts at: 2373 2427 2854 4292 668
Size: 54 427 1438 1925
Fragments arranged by size:
1925 1438 510 427 278 237 199 186
129 91 75 54
ApaI G_GGCC'C
Cuts at: 728 728
Size: 5549
ApaLI G'TGCA_C
Cuts at: 4387 4387
Size: 5549
ApoI r'AATT_y
Cuts at: 2553 4902 5158 2553
Size: 2349 256 2944
Fragments arranged by size:
2944 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5549
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5549
AvaI C'yCGr_G
Cuts at: 721 1373 1443 2569 721
Size: 652 70 1126 3701
Fragments arranged by size:
3701 1126 652 70
BamHI G'GATC_C
Cuts at: 2123 2574 2123
Size: 451 5098
BanI G'GyrC_C
Cuts at: 322 334 788 989 1040 2216 2565 2979 3227
Size: 12 454 201 51 1176 349 414 248
Cuts at: 3227 3262 322
Size: 35 2609
Fragments arranged by size:
2609 1176 454 414 349 248 201 51
35 12
BanII G_rGCy'C
Cuts at: 728 2563 3593 728
Size: 1835 1030 2684
Fragments arranged by size:
2684 1835 1030
BbeI G_GCGC'C
Cuts at: 2983 3231 2983
Size: 248 5301
BbsI GAAGACnn'nnnn_
Cuts at: 1681 1681
Size: 5549
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 790 824 1037 1071 790
Size: 34 213 34 5268
Fragments arranged by size:
5268 213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3447 4503 5549 3447
Size: 1056 1046 3447
Fragments arranged by size:
3447 1056 1046
BclI T'GATC_A
Cuts at: 2391 3069 2391
Size: 678 4871
BfrBI ATG'CAT
Cuts at: 1167 1167
Size: 5549
BglII A'GATC_T
Cuts at: 3064 5101 3064
Size: 2037 3512
BlpI GC'TnA_GC
Cuts at: 2661 2661
Size: 5549
Bme1580I G_kGCm'C
Cuts at: 728 3174 3267 4391 728
Size: 2446 93 1124 1886
Fragments arranged by size:
2446 1886 1124 93
BmgBI CAC'GTC
Cuts at: 443 696 1200 1719 443
Size: 253 504 519 4273
Fragments arranged by size:
4273 519 504 253
BmrI ACTGGGnnnn_n'
Cuts at: 138 1164 2493 2761 2842 3177 5321 138
Size: 1026 1329 268 81 335 2144 366
Fragments arranged by size:
2144 1329 1026 366 335 268 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3916 3916
Size: 5549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 926 980 1606 1648 2287 2395 926
Size: 54 626 42 639 108 4080
Fragments arranged by size:
4080 639 626 108 54 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2085 2725 3604 4318 4616 2085
Size: 640 879 714 298 3018
Fragments arranged by size:
3018 879 714 640 298
BsaI GGTCTCn'nnnn_
Cuts at: 1108 1838 5253 1108
Size: 730 3415 1404
Fragments arranged by size:
3415 1404 730
BsaAI yAC'GTr
Cuts at: 173 426 1671 1975 2387 2902 173
Size: 253 1245 304 412 515 2820
Fragments arranged by size:
2820 1245 515 412 304 253
BsaBI GATnn'nnATC
Cuts at: 6 575 3063 5100 6
Size: 569 2488 2037 455
Fragments arranged by size:
2488 2037 569 455
BsaHI Gr'CG_yC
Cuts at: 452 1948 2980 3228 3930 5426 452
Size: 1496 1032 248 702 1496 575
Fragments arranged by size:
1496 1496 1032 702 575 248
BsaWI w'CCGG_w
Cuts at: 2738 3259 3937 4348 4495 4769 2738
Size: 521 678 411 147 274 3518
Fragments arranged by size:
3518 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 640 670 640
Size: 30 5519
BseYI C'CCAG_C
Cuts at: 975 2192 2250 2974 3732 4397 4805 975
Size: 1217 58 724 758 665 408 1719
Fragments arranged by size:
1719 1217 758 724 665 408 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 863 863
Size: 5549
BsiEI CG_ry'CG
Cuts at: 104 810 3091 3137 4367 104
Size: 706 2281 46 1230 1286
Fragments arranged by size:
2281 1286 1230 706 46
BsiHKAI G_wGCw'C
Cuts at: 227 701 1619 2273 2563 3341 3531 4391 227
Size: 474 918 654 290 778 190 860 1385
Fragments arranged by size:
1385 918 860 778 654 474 290 190
BsmBI CGTCTCn'nnnn_
Cuts at: 211 1418 211
Size: 1207 4342
Bsp1286I G_dGCh'C
Cuts at: 227 701 728 1619 2273 2563 3174 3267 3341
Size: 474 27 891 654 290 611 93 74
Cuts at: 3341 3531 3593 4391 227
Size: 190 62 798 1385
Fragments arranged by size:
1385 891 798 654 611 474 290 190
93 74 62 27
BspHI T'CATG_A
Cuts at: 3981 3981
Size: 5549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 426 835 1090 1281 1856 2599 2813 3115 3496
Size: 409 255 191 575 743 214 302 381
Cuts at: 3496 426
Size: 2479
Fragments arranged by size:
2479 743 575 409 381 302 255 214
191
BsrBI CCG'CTC
Cuts at: 50 367 3841 3895 5379 5544 50
Size: 317 3474 54 1484 165 55
Fragments arranged by size:
3474 1484 317 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3461 3461
Size: 5549
BsrFI r'CCGG_y
Cuts at: 1678 3547 3728 1678
Size: 1869 181 3499
Fragments arranged by size:
3499 1869 181
BsrGI T'GTAC_A
Cuts at: 131 131
Size: 5549
BssSI C'ACGA_G
Cuts at: 3820 4528 3820
Size: 708 4841
BstAPI GCAn_nnn'nTGC
Cuts at: 668 867 1236 1602 1836 1863 2926 668
Size: 199 369 366 234 27 1063 3291
Fragments arranged by size:
3291 1063 369 366 234 199 27
BstBI TT'CG_AA
Cuts at: 1047 3910 1047
Size: 2863 2686
BstXI CCAn_nnnn'nTGG
Cuts at: 1324 1324
Size: 5549
BstYI r'GATC_y
Cuts at: 1 1859 2123 2574 3041 3064 3399 3645 3951
Size: 1858 264 451 467 23 335 246 306
Cuts at: 3951 3963 4049 5101 1
Size: 12 86 1052 449
Fragments arranged by size:
1858 1052 467 451 449 335 306 264
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2501 2501
Size: 5549
BtgI C'CryG_G
Cuts at: 107 253 713 932 1261 1323 1919 107
Size: 146 460 219 329 62 596 3737
Fragments arranged by size:
3737 596 460 329 219 146 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3499 3680 3843 3499
Size: 181 163 5205
Fragments arranged by size:
5205 181 163
BtsI GCAGTG_nn'
Cuts at: 2792 2792
Size: 5549
DraI TTT'AAA
Cuts at: 2202 2548 2202
Size: 346 5203
DrdI GACnn_nn'nnGTC
Cuts at: 912 1723 2329 3255 4599 912
Size: 811 606 926 1344 1862
Fragments arranged by size:
1862 1344 926 811 606
EaeI y'GGCC_r
Cuts at: 281 368 929 3134 3308 3699 3726 5071 281
Size: 87 561 2205 174 391 27 1345 759
Fragments arranged by size:
2205 1345 759 561 391 174 87 27
EagI C'GGCC_G
Cuts at: 3134 3134
Size: 5549
EarI CTCTTCn'nnn_
Cuts at: 1090 3572 3782 1090
Size: 2482 210 2857
Fragments arranged by size:
2857 2482 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3757 4499 4645 5359 3757
Size: 742 146 714 3947
Fragments arranged by size:
3947 742 714 146
EcoICRI GAG'CTC
Cuts at: 2561 2561
Size: 5549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 376 926 980 1246 1606 1648 2287 2395
Size: 194 550 54 266 360 42 639 108
Cuts at: 2395 3374 3806 4159 4821 182
Size: 979 432 353 662 910
Fragments arranged by size:
979 910 662 639 550 432 360 353
266 194 108 54 42
EcoO109I rG'GnC_Cy
Cuts at: 818 2424 2688 818
Size: 1606 264 3679
Fragments arranged by size:
3679 1606 264
EcoRI G'AATT_C
Cuts at: 2553 2553
Size: 5549
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 3568 2386
Size: 1182 4367
FspI TGC'GCA
Cuts at: 871 3330 871
Size: 2459 3090
HaeII r_GCGC'y
Cuts at: 347 1400 1994 2107 2315 2983 3231 4461 5232
Size: 1053 594 113 208 668 248 1230 771
Cuts at: 5232 347
Size: 664
Fragments arranged by size:
1230 1053 771 668 664 594 248 208
113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2389 2421 3391 3421 3423 3453 2389
Size: 32 970 30 2 30 4485
Fragments arranged by size:
4485 970 32 30 30 2
HincII GTy'rAC
Cuts at: 2588 2588
Size: 5549
KasI G'GCGC_C
Cuts at: 2979 3227 2979
Size: 248 5301
KpnI G_GTAC'C
Cuts at: 993 2220 2569 993
Size: 1227 349 3973
Fragments arranged by size:
3973 1227 349
MluI A'CGCG_T
Cuts at: 4982 4982
Size: 5549
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4308 4492 4308
Size: 184 5365
MscI TGG'CCA
Cuts at: 283 3310 283
Size: 3027 2522
MslI CAynn'nnrTG
Cuts at: 1322 1325 3538 3665 1322
Size: 3 2213 127 3206
Fragments arranged by size:
3206 2213 127 3
MspA1I CmG'CkG
Cuts at: 332 715 1179 1626 1661 1785 1854 2130 2660
Size: 383 464 447 35 124 69 276 530
Cuts at: 2660 2974 3334 4118 4363 332
Size: 314 360 784 245 1518
Fragments arranged by size:
1518 784 530 464 447 383 360 314
276 245 124 69 35
NaeI GCC'GGC
Cuts at: 3730 3730
Size: 5549
NarI GG'CG_CC
Cuts at: 2980 3228 2980
Size: 248 5301
NcoI C'CATG_G
Cuts at: 107 1323 107
Size: 1216 4333
NgoMIV G'CCGG_C
Cuts at: 3728 3728
Size: 5549
NsiI A_TGCA'T
Cuts at: 1169 1169
Size: 5549
NspI r_CATG'y
Cuts at: 132 296 678 842 1167 2602 3102 4705 132
Size: 164 382 164 325 1435 500 1603 976
Fragments arranged by size:
1603 1435 976 500 382 325 164 164
PasI CC'CwG_GG
Cuts at: 300 2211 300
Size: 1911 3638
PciI A'CATG_T
Cuts at: 128 4701 128
Size: 4573 976
PflMI CCAn_nnn'nTGG
Cuts at: 2460 2460
Size: 5549
PfoI T'CCnGG_A
Cuts at: 901 901
Size: 5549
PmeI GTTT'AAAC
Cuts at: 2548 2548
Size: 5549
PmlI CAC'GTG
Cuts at: 426 1671 1975 426
Size: 1245 304 4000
Fragments arranged by size:
4000 1245 304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 3995
Size: 32 5517
PpuMI rG'GwC_Cy
Cuts at: 818 818
Size: 5549
PspOMI G'GGCC_C
Cuts at: 724 724
Size: 5549
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 151 183 2061 2093 151
Size: 32 1878 32 3607
Fragments arranged by size:
3607 1878 32 32
PstI C_TGCA'G
Cuts at: 482 664 673 863 1241 1370 1793 1853 1859
Size: 182 9 190 378 129 423 60 6
Cuts at: 1859 2596 3281 482
Size: 737 685 2750
Fragments arranged by size:
2750 737 685 423 378 190 182 129
60 9 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5549
PvuII CAG'CTG
Cuts at: 1626 1661 1785 1854 2974 3334 1626
Size: 35 124 69 1120 360 3841
Fragments arranged by size:
3841 1120 360 124 69 35
RsrII CG'GwC_CG
Cuts at: 3744 3744
Size: 5549
SacI G_AGCT'C
Cuts at: 2563 2563
Size: 5549
SacII CC_GC'GG
Cuts at: 716 716
Size: 5549
SalI G'TCGA_C
Cuts at: 2586 2586
Size: 5549
SanDI GG'GwC_CC
Cuts at: 818 818
Size: 5549
SapI GCTCTTCn'nnn_
Cuts at: 3572 3782 3572
Size: 210 5339
SbfI CC_TGCA'GG
Cuts at: 482 2596 482
Size: 2114 3435
ScaI AGT'ACT
Cuts at: 2070 5066 2070
Size: 2996 2553
SexAI A'CCwGG_T
Cuts at: 1036 2219 1036
Size: 1183 4366
SfcI C'TryA_G
Cuts at: 33 478 660 669 859 1237 1357 1366 1789
Size: 445 182 9 190 378 120 9 423
Cuts at: 1789 1849 1855 2592 3277 4245 4436 33
Size: 60 6 737 685 968 191 1146
Fragments arranged by size:
1146 968 737 685 445 423 378 191
190 182 120 60 9 9 6
SfoI GGC'GCC
Cuts at: 2981 3229 2981
Size: 248 5301
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5549
SmaI CCC'GGG
Cuts at: 723 2571 723
Size: 1848 3701
SmlI C'TyrA_G
Cuts at: 2064 2704 3619 4333 4595 2064
Size: 640 915 714 262 3018
Fragments arranged by size:
3018 915 714 640 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5549
SphI G_CATG'C
Cuts at: 296 678 1167 2602 3102 296
Size: 382 489 1435 500 2743
Fragments arranged by size:
2743 1435 500 489 382
StyI C'CwwG_G
Cuts at: 107 1323 2318 2683 3037 107
Size: 1216 995 365 354 2619
Fragments arranged by size:
2619 1216 995 365 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 310 1407 1891 2264 3132 3265 3724 3933 310
Size: 1097 484 373 868 133 459 209 1926
Fragments arranged by size:
1926 1097 868 484 459 373 209 133
TatI w'GTAC_w
Cuts at: 131 2068 5064 131
Size: 1937 2996 616
Fragments arranged by size:
2996 1937 616
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1870 1902 5066 5098 1870
Size: 32 3164 32 2321
Fragments arranged by size:
3164 2321 32 32
Tth111I GACn'n_nGTC
Cuts at: 2286 3346 2286
Size: 1060 4489
XbaI T'CTAG_A
Cuts at: 2580 2580
Size: 5549
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3044 3044
Size: 5549
XmaI C'CCGG_G
Cuts at: 721 2569 721
Size: 1848 3701
XmnI GAAnn'nnTTC
Cuts at: 1508 1508
Size: 5549
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AloI AscI AvrII BaeI
BbvCI BglI BmtI Bpu10I BseRI BsiWI BsmI BspEI
BssHII BstEII Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PshAI PsiI PspXI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI