Restriction map for pF1KA0767
 (Linear) (Six-base) MAPSORT of: KIAA0767.seq  Check: 3428  from: 1  to: 1734



KIAA0767.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AatII G_ACGT'C

Cuts at:      0     914    1734 
   Size:        914     820
 
AccI GT'mk_AC

Cuts at:      0     692    1612    1734 
   Size:        692     920     122

  Fragments arranged by size: 

                 920     692     122
 
Acc65I G'GTAC_C

Cuts at:      0     117    1734 
   Size:        117    1617
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     415    1734 
   Size:        133     282    1319

  Fragments arranged by size: 

                1319     282     133
 
AfeI AGC'GCT

Cuts at:      0    1213    1734 
   Size:       1213     521
 
AflIII A'CryG_T

Cuts at:      0     695    1734 
   Size:        695    1039
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     529     561    1734 
   Size:        529      32    1173

  Fragments arranged by size: 

                1173     529      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     339     483    1011    1720    1734 
   Size:        339     144     528     709      14

  Fragments arranged by size: 

                 709     528     339     144      14
 
ApaLI G'TGCA_C

Cuts at:      0    1654    1734 
   Size:       1654      80
 
AvaI C'yCGr_G

Cuts at:      0     134     553    1137    1734 
   Size:        134     419     584     597

  Fragments arranged by size: 

                 597     584     419     134
 
BanI G'GyrC_C

Cuts at:      0     117     631    1645    1734 
   Size:        117     514    1014      89

  Fragments arranged by size: 

                1014     514     117      89
 
BanII G_rGCy'C

Cuts at:      0     293     854    1734 
   Size:        293     561     880

  Fragments arranged by size: 

                 880     561     293
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     878    1734 
   Size:        878     856
 
BbvCI CC'TCA_GC

Cuts at:      0     151    1707    1734 
   Size:        151    1556      27

  Fragments arranged by size: 

                1556     151      27
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     812    1734 
   Size:        812     922
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1039    1734 
   Size:       1039     695
 
BglII A'GATC_T

Cuts at:      0    1346    1734 
   Size:       1346     388
 
BlpI GC'TnA_GC

Cuts at:      0     422    1734 
   Size:        422    1312
 
Bme1580I G_kGCm'C

Cuts at:      0     839    1658    1734 
   Size:        839     819      76

  Fragments arranged by size: 

                 839     819      76
 
BmgBI CAC'GTC

Cuts at:      0     698    1734 
   Size:        698    1036
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     979    1197    1200    1734 
   Size:        391     588     218       3     534

  Fragments arranged by size: 

                 588     534     391     218       3
 
Bpu10I CC'TnA_GC

Cuts at:      0       4     151     586    1590    1707    1734 
   Size:          4     147     435    1004     117      27

  Fragments arranged by size: 

                1004     435     147     117      27       4
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     607    1189    1734 
   Size:        607     582     545

  Fragments arranged by size: 

                 607     582     545
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1669    1734 
   Size:       1669      65
 
BsaAI yAC'GTr

Cuts at:      0     531    1266    1734 
   Size:        531     735     468

  Fragments arranged by size: 

                 735     531     468
 
BsaHI Gr'CG_yC

Cuts at:      0     911    1304    1615    1734 
   Size:        911     393     311     119

  Fragments arranged by size: 

                 911     393     311     119
 
BsaWI w'CCGG_w

Cuts at:      0     209     293     542    1424    1734 
   Size:        209      84     249     882     310

  Fragments arranged by size: 

                 882     310     249     209      84
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     670     700    1734 
   Size:        670      30    1034

  Fragments arranged by size: 

                1034     670      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     160     301     424     571    1194    1312    1734 
   Size:        160     141     123     147     623     118     422

  Fragments arranged by size: 

                 623     422     160     147     141     123     118
 
BseYI C'CCAG_C

Cuts at:      0     743    1270    1734 
   Size:        743     527     464

  Fragments arranged by size: 

                 743     527     464
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403    1734 
   Size:        403    1331
 
BsiEI CG_ry'CG

Cuts at:      0    1283    1311    1734 
   Size:       1283      28     423

  Fragments arranged by size: 

                1283     423      28
 
BsiHKAI G_wGCw'C

Cuts at:      0     293    1463    1658    1734 
   Size:        293    1170     195      76

  Fragments arranged by size: 

                1170     293     195      76
 
BsmI GAATG_Cn'

Cuts at:      0      97     732    1734 
   Size:         97     635    1002

  Fragments arranged by size: 

                1002     635      97
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     609    1734 
   Size:        609    1125
 
Bsp1286I G_dGCh'C

Cuts at:      0     293     839     854    1463    1658    1734 
   Size:        293     546      15     609     195      76

  Fragments arranged by size: 

                 609     546     293     195      76      15
 
BspEI T'CCGG_A

Cuts at:      0     293    1734 
   Size:        293    1441
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     587    1734 
   Size:        587    1147
 
BsrBI CCG'CTC

Cuts at:      0     351     516    1635    1734 
   Size:        351     165    1119      99

  Fragments arranged by size: 

                1119     351     165      99
 
BsrDI GCAATG_nn'

Cuts at:      0     462     704    1734 
   Size:        462     242    1030

  Fragments arranged by size: 

                1030     462     242
 
BsrFI r'CCGG_y

Cuts at:      0     639    1278    1282    1306    1734 
   Size:        639     639       4      24     428

  Fragments arranged by size: 

                 639     639     428      24       4
 
BsrGI T'GTAC_A

Cuts at:      0     719    1734 
   Size:        719    1015
 
BssHII G'CGCG_C

Cuts at:      0    1146    1734 
   Size:       1146     588
 
BssSI C'ACGA_G

Cuts at:      0    1342    1636    1734 
   Size:       1342     294      98

  Fragments arranged by size: 

                1342     294      98
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     381     390    1734 
   Size:        381       9    1344

  Fragments arranged by size: 

                1344     381       9
 
BstBI TT'CG_AA

Cuts at:      0    1490    1734 
   Size:       1490     244
 
BstEII G'GTnAC_C

Cuts at:      0     244    1734 
   Size:        244    1490
 
BstYI r'GATC_y

Cuts at:      0    1346    1734 
   Size:       1346     388
 
BtgI C'CryG_G

Cuts at:      0     121     854    1079    1436    1649    1734 
   Size:        121     733     225     357     213      85

  Fragments arranged by size: 

                 733     357     225     213     121      85
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     786    1734 
   Size:        786     948
 
DraI TTT'AAA

Cuts at:      0    1128    1734 
   Size:       1128     606
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1251    1734 
   Size:       1251     483
 
EaeI y'GGCC_r

Cuts at:      0     185    1280    1734 
   Size:        185    1095     454

  Fragments arranged by size: 

                1095     454     185
 
EagI C'GGCC_G

Cuts at:      0    1280    1734 
   Size:       1280     454
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     375     831    1734 
   Size:        375     456     903

  Fragments arranged by size: 

                 903     456     375
 
EcoICRI GAG'CTC

Cuts at:      0     291    1734 
   Size:        291    1443
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     391     415     979    1197    1200    1734 
   Size:        133     258      24     564     218       3     534

  Fragments arranged by size: 

                 564     534     258     218     133      24       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1515    1734 
   Size:       1515     219
 
EcoO109I rG'GnC_Cy

Cuts at:      0     156     173    1508    1569    1734 
   Size:        156      17    1335      61     165

  Fragments arranged by size: 

                1335     165     156      61      17
 
FseI GG_CCGG'CC

Cuts at:      0    1286    1734 
   Size:       1286     448
 
HaeII r_GCGC'y

Cuts at:      0     471     593    1024    1215    1223    1404    1734 
   Size:        471     122     431     191       8     181     330

  Fragments arranged by size: 

                 471     431     330     191     181     122       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     228     260     670     702    1338    1370    1734 
   Size:        228      32     410      32     636      32     364

  Fragments arranged by size: 

                 636     410     364     228      32      32      32
 
HincII GTy'rAC

Cuts at:      0     602    1613    1734 
   Size:        602    1011     121

  Fragments arranged by size: 

                1011     602     121
 
HpaI GTT'AAC

Cuts at:      0     602    1734 
   Size:        602    1132
 
KpnI G_GTAC'C

Cuts at:      0     121    1734 
   Size:        121    1613
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     701    1734 
   Size:        701    1033
 
MscI TGG'CCA

Cuts at:      0     187    1734 
   Size:        187    1547
 
MslI CAynn'nnrTG

Cuts at:      0    1661    1734 
   Size:       1661      73
 
MspA1I CmG'CkG

Cuts at:      0     123     357     900    1377    1711    1734 
   Size:        123     234     543     477     334      23

  Fragments arranged by size: 

                 543     477     334     234     123      23
 
NaeI GCC'GGC

Cuts at:      0    1284    1734 
   Size:       1284     450
 
NcoI C'CATG_G

Cuts at:      0    1649    1734 
   Size:       1649      85
 
NgoMIV G'CCGG_C

Cuts at:      0    1282    1734 
   Size:       1282     452
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     812    1734 
   Size:        812     922
 
PfoI T'CCnGG_A

Cuts at:      0     197     956    1734 
   Size:        197     759     778

  Fragments arranged by size: 

                 778     759     197
 
PpuMI rG'GwC_Cy

Cuts at:      0     156     173    1508    1569    1734 
   Size:        156      17    1335      61     165

  Fragments arranged by size: 

                1335     165     156      61      17
 
PshAI GACnn'nnGTC

Cuts at:      0    1307    1734 
   Size:       1307     427
 
PstI C_TGCA'G

Cuts at:      0     500    1244    1734 
   Size:        500     744     490

  Fragments arranged by size: 

                 744     500     490
 
PvuII CAG'CTG

Cuts at:      0     900    1734 
   Size:        900     834
 
SacI G_AGCT'C

Cuts at:      0     293    1734 
   Size:        293    1441
 
SacII CC_GC'GG

Cuts at:      0     124    1734 
   Size:        124    1610
 
SalI G'TCGA_C

Cuts at:      0    1611    1734 
   Size:       1611     123
 
SanDI GG'GwC_CC

Cuts at:      0     156     173    1734 
   Size:        156      17    1561

  Fragments arranged by size: 

                1561     156      17
 
SfcI C'TryA_G

Cuts at:      0     496    1240    1734 
   Size:        496     744     494

  Fragments arranged by size: 

                 744     496     494
 
SmaI CCC'GGG

Cuts at:      0     136    1734 
   Size:        136    1598
 
SmlI C'TyrA_G

Cuts at:      0     622    1204    1734 
   Size:        622     582     530

  Fragments arranged by size: 

                 622     582     530
 
SnaBI TAC'GTA

Cuts at:      0    1266    1734 
   Size:       1266     468
 
StuI AGG'CCT

Cuts at:      0    1669    1734 
   Size:       1669      65
 
StyI C'CwwG_G

Cuts at:      0    1649    1734 
   Size:       1649      85
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1639    1734 
   Size:       1639      95
 
TatI w'GTAC_w

Cuts at:      0     719    1562    1691    1734 
   Size:        719     843     129      43

  Fragments arranged by size: 

                 843     719     129      43
 
Tth111I GACn'n_nGTC

Cuts at:      0     882    1251    1734 
   Size:        882     369     483

  Fragments arranged by size: 

                 882     483     369
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     454    1734 
   Size:        454    1280
 
XmaI C'CCGG_G

Cuts at:      0     134    1734 
   Size:        134    1600
 
XmnI GAAnn'nnTTC

Cuts at:      0     505     934     976    1734 
   Size:        505     429      42     758

  Fragments arranged by size: 

                 758     505     429      42
 
ZraI GAC'GTC

Cuts at:      0     912    1734 
   Size:        912     822
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AloI    AlwNI
    ApaLI     AvaI     BanI    BanII     BbsI    BbvCI    BciVI     BglI
    BglII     BlpI Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     DraI     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FseI    HaeII    Hin4I   HincII     HpaI
     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
    PflMI     PfoI    PpuMI    PshAI     PstI    PvuII     SacI    SacII
     SalI    SanDI     SfcI     SmaI     SmlI    SnaBI     StuI     StyI
    TaqII     TatI  Tth111I     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI    AflII     AgeI     AhdI     AleI     AlfI     ApaI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BcgI     BclI    BfrBI     BmrI     BmtI     BplI    BsaBI    BsiWI
    BspHI    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI   DraIII
     EarI    EcoRI    EcoRV     FalI     FspI    FspAI  HindIII     KasI
     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SpeI     SphI     SrfI
     SspI     SwaI     TstI     XbaI     XhoI
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