Restriction map for pF1KA0734
##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AccIII      AclI        AcsI        ApoI        AscI        
AseI        AsnI        Asp700I     BbeI        BbrPI       
BsaBI       BseAI       BsiWI       BsmI        BspDI       
BspEI       BspLU11I    BssHII      BstBI       BstXI       
ClaI        CpoI        DraI        DrdI        EcoRI       
EcoRV       EcoT22I     FseI        HindIII     HpaI        
KasI        MamI        MfeI        MluI        MroI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        SwaI        TfiI        Tsp509I     XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        454  GGTACC               G^GTACC
AflII        3132  CTTAAG               C^TTAAG
AflIII       2315  ACGTGT               A^C[GA][CT]GT
AgeI         3026  ACCGGT               A^CCGGT
AhdI         3319  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        704  GTGCAC               G^TGCAC
AocI         2907  CCTGAGG              CC^T[ACGT]AGG
ApaLI         704  GTGCAC               G^TGCAC
Asp718I       454  GGTACC               G^GTACC
AspEI        3319  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1804  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3363  CCTAGG               C^CTAGG
BcgI           56  GCACCGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1157  TGATCA               T^GATCA
BfaI         3364  CTAG                 C^TAG
BfrI         3132  CTTAAG               C^TTAAG
BlnI         3363  CCTAGG               C^CTAGG
BsaAI         579  CACGTA               [CT]AC^GT[GA]
BsaWI        3026  ACCGGT               [AT]^CCGG[AT]
BsmBI        2814  CGTCTC               CGTCTC(1/5)
BsrDI        1689  CATTGC               GCAATG(2/0)
BssSI        2434  CTCGTG               CACGAG(-5/-1)
Bst1107I     3232  GTATAC               GTA^TAC
BstZ17I      3232  GTATAC               GTA^TAC
Bsu36I       2907  CCTGAGG              CC^T[ACGT]AGG
DraIII       2569  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2381  CGGCCG               C^GGCCG
Eam1105I     3319  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2381  CGGCCG               C^GGCCG
Eco52I       2381  CGGCCG               C^GGCCG
Eco81I       2907  CCTGAGG              CC^T[ACGT]AGG
EcoNI        2186  CCTCCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1157  TGATCA               T^GATCA
KpnI          454  GGTACC               GGTAC^C
KspI         3101  CCGCGG               CCGC^GG
MaeI         3364  CTAG                 C^TAG
NaeI         3443  GCCGGC               GCC^GGC
NcoI          458  CCATGG               C^CATGG
NgoMIV       3443  GCCGGC               G^CCGGC
PaeR7I        319  CTCGAG               C^TCGAG
PflFI        1804  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3026  ACCGGT               A^CCGGT
PshAI        1744  GACAAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII        3101  CCGCGG               CCGC^GG
SphI          659  GCATGC               GCATG^C
StuI         2456  AGGCCT               AGG^CCT
Tth111I      1804  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          319  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AatII        1876  GACGTC               GACGT^C
             2812  GACGTC              
Aor51HI       719  AGCGCT               AGC^GCT
             2018  AGCGCT              
AviII         332  TGCGCA               TGC^GCA
             2438  TGCGCA              
BamHI         366  GGATCC               G^GATCC
             1733  GGATCC              
BbsI         3210  GAAGAC               GAAGAC(2/6)
             1879  GTCTTC              
BciVI        1173  GGATAC               GTATCC(6/5)
              898  GTATCC              
BglII        1655  AGATCT               A^GATCT
             3463  AGATCT              
BpuAI        3210  GAAGAC               GAAGAC(2/6)
             1879  GTCTTC              
BsaI            3  GAGACC               GGTCTC(1/5)
             2221  GGTCTC              
BsiEI        1083  CGACCG               CG[GA][CT]^CG
             2381  CGGCCG              
Bsp1407I      431  TGTACA               T^GTACA
             2622  TGTACA              
BspHI         885  TCATGA               T^CATGA
             2681  TCATGA              
BsrBI        2052  CCGCTC               CCGCTC(-3/-3)
             2677  CCGCTC              
BsrGI         431  TGTACA               T^GTACA
             2622  TGTACA              
Eco47III      719  AGCGCT               AGC^GCT
             2018  AGCGCT              
FspI          332  TGCGCA               TGC^GCA
             2438  TGCGCA              
HincII        108  GTCGAC               GT[CT]^[GA]AC
             1081  GTCGAC              
HindII        108  GTCGAC               GT[CT]^[GA]AC
             1081  GTCGAC              
MseI          125  TTAA                 T^TAA
             3133  TTAA                
PflMI        1274  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1593  CCACCGCCTGG         
RcaI          885  TCATGA               T^CATGA
             2681  TCATGA              
SalI          108  GTCGAC               G^TCGAC
             1081  GTCGAC              
SapI          792  GAAGAGC              GCTCTTC(1/4)
             1635  GCTCTTC             
SexAI         470  ACCAGGT              A^CC[AT]GGT
             1142  ACCTGGT             
SmaI            8  CCCGGG               CCC^GGG
              241  CCCGGG              
SspBI         431  TGTACA               T^GTACA
             2622  TGTACA              
Tru9I         125  TTAA                 T^TAA
             3133  TTAA                
Van91I       1274  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1593  CCACCGCCTGG         
XmaCI           8  CCCGGG               C^CCGGG
              241  CCCGGG              
XmaI            8  CCCGGG               C^CCGGG
              241  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvCI       BbvI        BcnI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstAPI      BstEII      
BstF5I      BstNI       BstPI       BstUI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XcmI        XhoII       
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