Restriction map for pF1KA0700
 (Linear) (Six-base) MAPSORT of: KIAA0700.seq  Check: 3031  from: 1  to: 3390



KIAA0700.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     417    2781    3150    3390 
   Size:        417    2364     369     240

  Fragments arranged by size: 

                2364     417     369     240
 
AatII G_ACGT'C

Cuts at:      0    2618    3390 
   Size:       2618     772
 
AccI GT'mk_AC

Cuts at:      0    1796    2216    3390 
   Size:       1796     420    1174

  Fragments arranged by size: 

                1796    1174     420
 
Acc65I G'GTAC_C

Cuts at:      0     868    3297    3390 
   Size:        868    2429      93

  Fragments arranged by size: 

                2429     868      93
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     647     973    1645    2311    2431    2644    3390 
   Size:        647     326     672     666     120     213     746

  Fragments arranged by size: 

                 746     672     666     647     326     213     120
 
AfeI AGC'GCT

Cuts at:      0     805    1375    1904    2737    3390 
   Size:        805     570     529     833     653

  Fragments arranged by size: 

                 833     805     653     570     529
 
AflIII A'CryG_T

Cuts at:      0     252     572    3125    3390 
   Size:        252     320    2553     265

  Fragments arranged by size: 

                2553     320     265     252
 
AleI CACnn'nnGTG

Cuts at:      0     646    1361    3390 
   Size:        646     715    2029

  Fragments arranged by size: 

                2029     715     646
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2597    3390 
   Size:       2597     793
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1300    1332    2668    2700    3390 
   Size:       1300      32    1336      32     690

  Fragments arranged by size: 

                1336    1300     690      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     702    1011    1887    1974    2013    2274    2322    2862 
   Size:        702     309     876      87      39     261      48     540

Cuts at:   2862    3171    3218    3390 
   Size:        309      47     172

  Fragments arranged by size: 

                 876     702     540     309     309     261     172      87
                  48      47      39
 
ApaI G_GGCC'C

Cuts at:      0    3003    3390 
   Size:       3003     387
 
ApaLI G'TGCA_C

Cuts at:      0     115    3325    3331    3390 
   Size:        115    3210       6      59

  Fragments arranged by size: 

                3210     115      59       6
 
ApoI r'AATT_y

Cuts at:      0     384     481     919    1060    3390 
   Size:        384      97     438     141    2330

  Fragments arranged by size: 

                2330     438     384     141      97
 
AvaI C'yCGr_G

Cuts at:      0      84    3390 
   Size:         84    3306
 
BamHI G'GATC_C

Cuts at:      0    1192    3390 
   Size:       1192    2198
 
BanI G'GyrC_C

Cuts at:      0      34      67     429     456     868    1458    1957    2023 
   Size:         34      33     362      27     412     590     499      66

Cuts at:   2023    2103    2662    3297    3390 
   Size:         80     559     635      93

  Fragments arranged by size: 

                 635     590     559     499     412     362      93      80
                  66      34      33      27
 
BanII G_rGCy'C

Cuts at:      0      91    1031    2639    2813    3003    3025    3390 
   Size:         91     940    1608     174     190      22     365

  Fragments arranged by size: 

                1608     940     365     190     174      91      22
 
BbeI G_GCGC'C

Cuts at:      0      71    1462    2027    2107    3390 
   Size:         71    1391     565      80    1283

  Fragments arranged by size: 

                1391    1283     565      80      71
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     131    1339    1472    3390 
   Size:        131    1208     133    1918

  Fragments arranged by size: 

                1918    1208     133     131
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2848    2882    3390 
   Size:       2848      34     508

  Fragments arranged by size: 

                2848     508      34
 
BclI T'GATC_A

Cuts at:      0    2795    3390 
   Size:       2795     595
 
BfrBI ATG'CAT

Cuts at:      0    1174    3390 
   Size:       1174    2216
 
BglI GCCn_nnn'nGGC

Cuts at:      0      50    3390 
   Size:         50    3340
 
BglII A'GATC_T

Cuts at:      0    1673    2294    3390 
   Size:       1673     621    1096

  Fragments arranged by size: 

                1673    1096     621
 
BlpI GC'TnA_GC

Cuts at:      0      61    2732    3082    3390 
   Size:         61    2671     350     308

  Fragments arranged by size: 

                2671     350     308      61
 
Bme1580I G_kGCm'C

Cuts at:      0     119     461    1209    1839    1960    1991    3003    3329 
   Size:        119     342     748     630     121      31    1012     326

Cuts at:   3329    3335    3390 
   Size:          6      55

  Fragments arranged by size: 

                1012     748     630     342     326     121     119      55
                  31       6
 
BmgBI CAC'GTC

Cuts at:      0     253    1508    3063    3390 
   Size:        253    1255    1555     327

  Fragments arranged by size: 

                1555    1255     327     253
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2078    2414    3390 
   Size:       2078     336     976

  Fragments arranged by size: 

                2078     976     336
 
BmtI G_CTAG'C

Cuts at:      0    3254    3390 
   Size:       3254     136
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1836    3390 
   Size:       1836    1554
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     260     484     580    1417    1909    2265    2344 
   Size:        217      43     224      96     837     492     356      79

Cuts at:   2344    2455    2500    3244    3390 
   Size:        111      45     744     146

  Fragments arranged by size: 

                 837     744     492     356     224     217     146     111
                  96      79      45      43
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1564    1690    2944    3390 
   Size:       1564     126    1254     446

  Fragments arranged by size: 

                1564    1254     446     126
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     725    1290    2704    3390 
   Size:        725     565    1414     686

  Fragments arranged by size: 

                1414     725     686     565
 
BsaAI yAC'GTr

Cuts at:      0     120     575    1920    2547    2874    3330    3390 
   Size:        120     455    1345     627     327     456      60

  Fragments arranged by size: 

                1345     627     456     455     327     120      60
 
BsaHI Gr'CG_yC

Cuts at:      0      68     128    1459    2024    2104    2615    3390 
   Size:         68      60    1331     565      80     511     775

  Fragments arranged by size: 

                1331     775     565     511      80      68      60
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1000    1030    2668    2698    3390 
   Size:       1000      30    1638      30     692

  Fragments arranged by size: 

                1638    1000     692      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     646     811    1360    1654    2047    2146    2452    2839 
   Size:        646     165     549     294     393      99     306     387

Cuts at:   2839    3217    3295    3390 
   Size:        378      78      95

  Fragments arranged by size: 

                 646     549     393     387     378     306     294     165
                  99      95      78
 
BseYI C'CCAG_C

Cuts at:      0      74    1655    1990    2187    3390 
   Size:         74    1581     335     197    1203

  Fragments arranged by size: 

                1581    1203     335     197      74
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     428     553     781     821    1257    1456    1960    2248 
   Size:        428     125     228      40     436     199     504     288

Cuts at:   2248    2578    2593    3184    3280    3382    3390 
   Size:        330      15     591      96     102       8

  Fragments arranged by size: 

                 591     504     436     428     330     288     228     199
                 125     102      96      40      15       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     119     278     776     794    1031    1808    2281    2639 
   Size:        119     159     498      18     237     777     473     358

Cuts at:   2639    2813    3025    3329    3335    3390 
   Size:        174     212     304       6      55

  Fragments arranged by size: 

                 777     498     473     358     304     237     212     174
                 159     119      55      18       6
 
BsiWI C'GTAC_G

Cuts at:      0    1340    3390 
   Size:       1340    2050
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1393    3390 
   Size:       1393    1997
 
Bsp1286I G_dGCh'C

Cuts at:      0      91     119     278     461     776     794    1031    1209 
   Size:         91      28     159     183     315      18     237     178

Cuts at:   1209    1808    1839    1960    1991    2281    2639    2813    3003 
   Size:        599      31     121      31     290     358     174     190

Cuts at:   3003    3025    3329    3335    3390 
   Size:         22     304       6      55

  Fragments arranged by size: 

                 599     358     315     304     290     237     190     183
                 178     174     159     121      91      55      31      31
                  28      22      18       6
 
BspHI T'CATG_A

Cuts at:      0     203    2798    3390 
   Size:        203    2595     592

  Fragments arranged by size: 

                2595     592     203
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     417     686    2781    3150    3390 
   Size:        417     269    2095     369     240

  Fragments arranged by size: 

                2095     417     369     269     240
 
BsrBI CCG'CTC

Cuts at:      0      65    2091    2346    3390 
   Size:         65    2026     255    1044

  Fragments arranged by size: 

                2026    1044     255      65
 
BsrDI GCAATG_nn'

Cuts at:      0    2579    3390 
   Size:       2579     811
 
BsrFI r'CCGG_y

Cuts at:      0      37     111     605    1877    1932    2100    2975    3390 
   Size:         37      74     494    1272      55     168     875     415

  Fragments arranged by size: 

                1272     875     494     415     168      74      55      37
            
 
BssSI C'ACGA_G

Cuts at:      0     100     271     597    2602    3390 
   Size:        100     171     326    2005     788

  Fragments arranged by size: 

                2005     788     326     171     100
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     429     759    3339    3390 
   Size:        429     330    2580      51

  Fragments arranged by size: 

                2580     429     330      51
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2427    3390 
   Size:       2427     963
 
BstYI r'GATC_y

Cuts at:      0     155     563    1138    1192    1673    1883    2294    3390 
   Size:        155     408     575      54     481     210     411    1096

  Fragments arranged by size: 

                1096     575     481     411     408     210     155      54
            
 
BtgI C'CryG_G

Cuts at:      0      47     396     476    2396    2558    3390 
   Size:         47     349      80    1920     162     832

  Fragments arranged by size: 

                1920     832     349     162      80      47
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     870     975    2624    3390 
   Size:        870     105    1649     766

  Fragments arranged by size: 

                1649     870     766     105
 
BtsI GCAGTG_nn'

Cuts at:      0     899    1245    1839    3390 
   Size:        899     346     594    1551

  Fragments arranged by size: 

                1551     899     594     346
 
ClaI AT'CG_AT

Cuts at:      0     230    3390 
   Size:        230    3160
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1221    1256    3390 
   Size:       1221      35    2134

  Fragments arranged by size: 

                2134    1221      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     759    2604    3390 
   Size:        759    1845     786

  Fragments arranged by size: 

                1845     786     759
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1282    3390 
   Size:       1282    2108
 
EaeI y'GGCC_r

Cuts at:      0     607     650    1409    1934    2159    2744    3390 
   Size:        607      43     759     525     225     585     646

  Fragments arranged by size: 

                 759     646     607     585     525     225      43
 
EarI CTCTTCn'nnn_

Cuts at:      0     271     623     664     949    1003    1631    2002    3390 
   Size:        271     352      41     285      54     628     371    1388

  Fragments arranged by size: 

                1388     628     371     352     285     271      54      41
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     814     931    2959    3172    3390 
   Size:        814     117    2028     213     218

  Fragments arranged by size: 

                2028     814     218     213     117
 
EcoICRI GAG'CTC

Cuts at:      0    1029    2637    2811    3023    3390 
   Size:       1029    1608     174     212     367

  Fragments arranged by size: 

                1608    1029     367     212     174
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     260     484     580     647     973    1417    1645 
   Size:        217      43     224      96      67     326     444     228

Cuts at:   1645    1909    2265    2311    2344    2431    2455    2500    2644 
   Size:        264     356      46      33      87      24      45     144

Cuts at:   2644    3244    3390 
   Size:        600     146

  Fragments arranged by size: 

                 600     444     356     326     264     228     224     217
                 146     144      96      87      67      46      45      43
                  33      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2479    3259    3390 
   Size:       2479     780     131

  Fragments arranged by size: 

                2479     780     131
 
EcoO109I rG'GnC_Cy

Cuts at:      0     675    1557    1755    2110    2141    2841    3000    3140 
   Size:        675     882     198     355      31     700     159     140

Cuts at:   3140    3390 
   Size:        250

  Fragments arranged by size: 

                 882     700     675     355     250     198     159     140
                  31
 
EcoRI G'AATT_C

Cuts at:      0     384     481    3390 
   Size:        384      97    2909

  Fragments arranged by size: 

                2909     384      97
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     177    2160    3390 
   Size:        177    1983    1230

  Fragments arranged by size: 

                1983    1230     177
 
FseI GG_CCGG'CC

Cuts at:      0     609    3390 
   Size:        609    2781
 
HaeII r_GCGC'y

Cuts at:      0      71     807    1377    1462    1527    1906    2027    2107 
   Size:         71     736     570      85      65     379     121      80

Cuts at:   2107    2739    3056    3390 
   Size:        632     317     334

  Fragments arranged by size: 

                 736     632     570     379     334     317     121      85
                  80      71      65
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     667     699    1000    1032    1159    1191    2887    2919 
   Size:        667      32     301      32     127      32    1696      32

Cuts at:   2919    3390 
   Size:        471

  Fragments arranged by size: 

                1696     667     471     301     127      32      32      32
                  32
 
KasI G'GCGC_C

Cuts at:      0      67    1458    2023    2103    3390 
   Size:         67    1391     565      80    1287

  Fragments arranged by size: 

                1391    1287     565      80      67
 
KpnI G_GTAC'C

Cuts at:      0     872    3301    3390 
   Size:        872    2429      89

  Fragments arranged by size: 

                2429     872      89
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     273    1406    1490    3390 
   Size:        273    1133      84    1900

  Fragments arranged by size: 

                1900    1133     273      84
 
MscI TGG'CCA

Cuts at:      0     652    1411    3390 
   Size:        652     759    1979

  Fragments arranged by size: 

                1979     759     652
 
MslI CAynn'nnrTG

Cuts at:      0     646    1361    1864    3390 
   Size:        646     715     503    1526

  Fragments arranged by size: 

                1526     715     646     503
 
MspA1I CmG'CkG

Cuts at:      0      23      32      49      74     591     986    1361    1600 
   Size:         23       9      17      25     517     395     375     239

Cuts at:   1600    1971    2013    2041    2592    2668    2690    3218    3390 
   Size:        371      42      28     551      76      22     528     172

  Fragments arranged by size: 

                 551     528     517     395     375     371     239     172
                  76      42      28      25      23      22      17       9
            
 
NaeI GCC'GGC

Cuts at:      0      39     607    1934    2102    3390 
   Size:         39     568    1327     168    1288

  Fragments arranged by size: 

                1327    1288     568     168      39
 
NarI GG'CG_CC

Cuts at:      0      68    1459    2024    2104    3390 
   Size:         68    1391     565      80    1286

  Fragments arranged by size: 

                1391    1286     565      80      68
 
NcoI C'CATG_G

Cuts at:      0    2396    2558    3390 
   Size:       2396     162     832

  Fragments arranged by size: 

                2396     832     162
 
NgoMIV G'CCGG_C

Cuts at:      0      37     605    1932    2100    3390 
   Size:         37     568    1327     168    1290

  Fragments arranged by size: 

                1327    1290     568     168      37
 
NheI G'CTAG_C

Cuts at:      0    3250    3390 
   Size:       3250     140
 
NsiI A_TGCA'T

Cuts at:      0    1176    3390 
   Size:       1176    2214
 
NspI r_CATG'y

Cuts at:      0     624    3129    3390 
   Size:        624    2505     261

  Fragments arranged by size: 

                2505     624     261
 
PasI CC'CwG_GG

Cuts at:      0    1497    2064    3390 
   Size:       1497     567    1326

  Fragments arranged by size: 

                1497    1326     567
 
PciI A'CATG_T

Cuts at:      0    3125    3390 
   Size:       3125     265
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1011    2862    3390 
   Size:       1011    1851     528

  Fragments arranged by size: 

                1851    1011     528
 
PfoI T'CCnGG_A

Cuts at:      0     194     237     557    1394    1886    2456    2813    3025 
   Size:        194      43     320     837     492     570     357     212

Cuts at:   3025    3390 
   Size:        365

  Fragments arranged by size: 

                 837     570     492     365     357     320     212     194
                  43
 
PmlI CAC'GTG

Cuts at:      0    3330    3390 
   Size:       3330      60
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     622     654    2668    2700    3390 
   Size:        622      32    2014      32     690

  Fragments arranged by size: 

                2014     690     622      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     675    2110    2841    3140    3390 
   Size:        675    1435     731     299     250

  Fragments arranged by size: 

                1435     731     675     299     250
 
PspOMI G'GGCC_C

Cuts at:      0    2999    3390 
   Size:       2999     391
 
PstI C_TGCA'G

Cuts at:      0     894     917    1037    2780    2951    3390 
   Size:        894      23     120    1743     171     439

  Fragments arranged by size: 

                1743     894     439     171     120      23
 
PvuII CAG'CTG

Cuts at:      0     591    1971    2013    2041    2592    3390 
   Size:        591    1380      42      28     551     798

  Fragments arranged by size: 

                1380     798     591     551      42      28
 
RsrII CG'GwC_CG

Cuts at:      0    2333    3390 
   Size:       2333    1057
 
SacI G_AGCT'C

Cuts at:      0    1031    2639    2813    3025    3390 
   Size:       1031    1608     174     212     365

  Fragments arranged by size: 

                1608    1031     365     212     174
 
SacII CC_GC'GG

Cuts at:      0      50    3390 
   Size:         50    3340
 
SanDI GG'GwC_CC

Cuts at:      0    3140    3390 
   Size:       3140     250
 
SapI GCTCTTCn'nnn_

Cuts at:      0     271     949    3390 
   Size:        271     678    2441

  Fragments arranged by size: 

                2441     678     271
 
ScaI AGT'ACT

Cuts at:      0    2443    3390 
   Size:       2443     947
 
SexAI A'CCwGG_T

Cuts at:      0     146    3390 
   Size:        146    3244
 
SfcI C'TryA_G

Cuts at:      0     546     890     913    1033    2217    2776    2947    3390 
   Size:        546     344      23     120    1184     559     171     443

  Fragments arranged by size: 

                1184     559     546     443     344     171     120      23
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      50    3390 
   Size:         50    3340
 
SfoI GGC'GCC

Cuts at:      0      69    1460    2025    2105    3390 
   Size:         69    1391     565      80    1285

  Fragments arranged by size: 

                1391    1285     565      80      69
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2975    3390 
   Size:       2975     415
 
SmlI C'TyrA_G

Cuts at:      0    1579    1705    2959    3390 
   Size:       1579     126    1254     431

  Fragments arranged by size: 

                1579    1254     431     126
 
StyI C'CwwG_G

Cuts at:      0    1631    1751    2396    2558    3390 
   Size:       1631     120     645     162     832

  Fragments arranged by size: 

                1631     832     645     162     120
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     551    1140    2350    2908    3265    3390 
   Size:        551     589    1210     558     357     125

  Fragments arranged by size: 

                1210     589     558     551     357     125
 
TatI w'GTAC_w

Cuts at:      0    2441    3365    3390 
   Size:       2441     924      25

  Fragments arranged by size: 

                2441     924      25
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1627    1659    3390 
   Size:       1627      32    1731

  Fragments arranged by size: 

                1731    1627      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     937    1390    2212    3390 
   Size:        937     453     822    1178

  Fragments arranged by size: 

                1178     937     822     453
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     891    2565    2811    3390 
   Size:        891    1674     246     579

  Fragments arranged by size: 

                1674     891     579     246
 
XmnI GAAnn'nnTTC

Cuts at:      0     211    2758    3390 
   Size:        211    2547     632

  Fragments arranged by size: 

                2547     632     211
 
ZraI GAC'GTC

Cuts at:      0    2616    3390 
   Size:       2616     774
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI    HaeII    Hin4I
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     ScaI    SexAI     SfcI     SfiI
     SfoI    SgrAI     SmlI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AgeI     AhdI     AscI     AseI    AsiSI    AvrII
     BaeI    BbvCI    BciVI   Bpu10I    BsaBI    BsaWI    BsiEI     BsmI
    BspEI    BsrGI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I     DraI
     EagI    EcoRV     FspI    FspAI   HincII  HindIII     HpaI     MfeI
     MluI     NdeI     NotI     NruI     PacI     PmeI    PshAI     PsiI
    PspXI     PsrI     PvuI     SalI     SbfI     SgfI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     XbaI     XhoI
     XmaI
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