Restriction map for pF1KA0676
 (Circular) (Six-base) MAPSORT of: pF1KA0676.seq  Check: 6076  from: 1  to: 6863



pF1KA0676  6863 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2199    2199 
   Size:       6863
 
AccI GT'mk_AC

Cuts at:    317    2171    3683    3901     317 
   Size:       1854    1512     218    3279

  Fragments arranged by size: 

                3279    1854    1512     218
 
Acc65I G'GTAC_C

Cuts at:   3879    3879 
   Size:       6863
 
AclI AA'CG_TT

Cuts at:   6703    6703 
   Size:       6863
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    553     958    1495    1645    3388    4688    5120    5473    6135 
   Size:        405     537     150    1743    1300     432     353     662

Cuts at:   6135     553 
   Size:       1281

  Fragments arranged by size: 

                1743    1300    1281     662     537     432     405     353
                 150
 
AflIII A'CryG_T

Cuts at:    715    3215    3560    4215    6015    6296     715 
   Size:       2500     345     655    1800     281    1282

  Fragments arranged by size: 

                2500    1800    1282     655     345     281
 
AhdI GACnn_n'nnGTC

Cuts at:   3561    3561 
   Size:       6863
 
AleI CACnn'nnGTG

Cuts at:   1999    2348    1999 
   Size:        349    6514
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2366    2366 
   Size:       6863
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3799    3831    3799 
   Size:         32    6831
 
AlwNI CAG_nnn'CTG

Cuts at:    399     599     864    1470    2085    2250    2478    3246    3341 
   Size:        200     265     606     615     165     228     768      95

Cuts at:   3341    3534    3717    4168    5606     399 
   Size:        193     183     451    1438    1656

  Fragments arranged by size: 

                1656    1438     768     615     606     451     265     228
                 200     193     183     165      95
 
ApaLI G'TGCA_C

Cuts at:   5701    5701 
   Size:       6863
 
ApoI r'AATT_y

Cuts at:   2425    3867    6216    6472    2425 
   Size:       1442    2349     256    2816

  Fragments arranged by size: 

                2816    2349    1442     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6863
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6863
 
AvaI C'yCGr_G

Cuts at:    508     748     956    1702    1792    3883     508 
   Size:        240     208     746      90    2091    3488

  Fragments arranged by size: 

                3488    2091     746     240     208      90
 
AvrII C'CTAG_G

Cuts at:    901     901 
   Size:       6863
 
BamHI G'GATC_C

Cuts at:   3888    3888 
   Size:       6863
 
BanI G'GyrC_C

Cuts at:    238    1449    3676    3879    4293    4541    4576     238 
   Size:       1211    2227     203     414     248      35    2525

  Fragments arranged by size: 

                2525    2227    1211     414     248     203      35
 
BanII G_rGCy'C

Cuts at:    121     785    2013    2042    2405    2908    3520    3598    3877 
   Size:        664    1228      29     363     503     612      78     279

Cuts at:   3877    4907     121 
   Size:       1030    2077

  Fragments arranged by size: 

                2077    1228    1030     664     612     503     363     279
                  78      29
 
BbeI G_GCGC'C

Cuts at:   1453    4297    4545    1453 
   Size:       2844     248    3771

  Fragments arranged by size: 

                3771    2844     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    220    1355    2453    3233    3617     220 
   Size:       1135    1098     780     384    3466

  Fragments arranged by size: 

                3466    1135    1098     780     384
 
BbvCI CC'TCA_GC

Cuts at:   1417    2134    1417 
   Size:        717    6146
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1783    1817    3094    3128    1783 
   Size:         34    1277      34    5518

  Fragments arranged by size: 

                5518    1277      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   1642    4761    5817    6863    1642 
   Size:       3119    1056    1046    1642

  Fragments arranged by size: 

                3119    1642    1056    1046
 
BclI T'GATC_A

Cuts at:   2801    4383    2801 
   Size:       1582    5281
 
BglI GCCn_nnn'nGGC

Cuts at:    274     847     912    1522    2932    3268     274 
   Size:        573      65     610    1410     336    3869

  Fragments arranged by size: 

                3869    1410     610     573     336      65
 
BglII A'GATC_T

Cuts at:   2988    4378    6415    2988 
   Size:       1390    2037    3436

  Fragments arranged by size: 

                3436    2037    1390
 
BlpI GC'TnA_GC

Cuts at:    115     527    1715    2359    3975     115 
   Size:        412    1188     644    1616    3003

  Fragments arranged by size: 

                3003    1616    1188     644     412
 
Bme1580I G_kGCm'C

Cuts at:    241     270     617    1617    4488    4581    5705     241 
   Size:         29     347    1000    2871      93    1124    1399

  Fragments arranged by size: 

                2871    1399    1124    1000     347      93      29
 
BmgBI CAC'GTC

Cuts at:    249     249 
   Size:       6863
 
BmrI ACTGGGnnnn_n'

Cuts at:    375    2625    4075    4156    4491    6635     375 
   Size:       2250    1450      81     335    2144     603

  Fragments arranged by size: 

                2250    2144    1450     603     335      81
 
BmtI G_CTAG'C

Cuts at:   2609    2609 
   Size:       6863
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2345    2976    5230    2345 
   Size:        631    2254    3978

  Fragments arranged by size: 

                3978    2254     631
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    246     376     742     904    1164    1273    2082    2587    2689 
   Size:        130     366     162     260     109     809     505     102

Cuts at:   2689    2950    3866     246 
   Size:        261     916    3243

  Fragments arranged by size: 

                3243     916     809     505     366     261     260     162
                 130     109     102
 
Bpu10I CC'TnA_GC

Cuts at:   1417    2134    1417 
   Size:        717    6146
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2851    3002    3766    4039    4918    5632    5930    2851 
   Size:        151     764     273     879     714     298    3784

  Fragments arranged by size: 

                3784     879     764     714     298     273     151
 
BsaI GGTCTCn'nnnn_

Cuts at:    808     938    1766    3416    6567     808 
   Size:        130     828    1650    3151    1104

  Fragments arranged by size: 

                3151    1650    1104     828     130
 
BsaAI yAC'GTr

Cuts at:   4216    4216 
   Size:       6863
 
BsaBI GATnn'nnATC

Cuts at:      6    1078    2993    4377    6414       6 
   Size:       1072    1915    1384    2037     455

  Fragments arranged by size: 

                2037    1915    1384    1072     455
 
BsaHI Gr'CG_yC

Cuts at:    192     953    1042    1450    2231    4294    4542    5244    6740 
   Size:        761      89     408     781    2063     248     702    1496

Cuts at:   6740     192 
   Size:        315

  Fragments arranged by size: 

                2063    1496     781     761     702     408     315     248
                  89
 
BsaWI w'CCGG_w

Cuts at:   2678    3093    4052    4573    5251    5662    5809    6083    2678 
   Size:        415     959     521     678     411     147     274    3458

  Fragments arranged by size: 

                3458     959     678     521     415     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2000    2030    3075    3105    3799    3829    2000 
   Size:         30    1045      30     694      30    5034

  Fragments arranged by size: 

                5034    1045     694      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    139    1129    1364    1558    1654    1774    1948    1951    2321 
   Size:        990     235     194      96     120     174       3     370

Cuts at:   2321    2529    2952    2966    3076    3083    3088    3367     139 
   Size:        208     423      14     110       7       5     279    3635

  Fragments arranged by size: 

                3635     990     423     370     279     235     208     194
                 174     120     110      96      14       7       5       3
            
 
BseYI C'CCAG_C

Cuts at:    672    1192    1368    1404    1818    2250    2286    2653    2895 
   Size:        520     176      36     414     432      36     367     242

Cuts at:   2895    3411    3527    4288    5046    5711    6119     672 
   Size:        516     116     761     758     665     408    1416

  Fragments arranged by size: 

                1416     761     758     665     520     516     432     414
                 408     367     242     176     116      36      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    277     718    1014    1042    1288    1762    2643     277 
   Size:        441     296      28     246     474     881    4497

  Fragments arranged by size: 

                4497     881     474     441     296     246      28
 
BsiEI CG_ry'CG

Cuts at:    104    2643    4405    4451    5681     104 
   Size:       2539    1762      46    1230    1286

  Fragments arranged by size: 

                2539    1762    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    785    1799    1916    2042    2405    2876    3877    4655    4845 
   Size:       1014     117     126     363     471    1001     778     190

Cuts at:   4845    5705     785 
   Size:        860    1943

  Fragments arranged by size: 

                1943    1014    1001     860     778     471     363     190
                 126     117
 
BsmBI CGTCTCn'nnnn_

Cuts at:    491    3142     491 
   Size:       2651    4212
 
Bsp1286I G_dGCh'C

Cuts at:    121     241     270     617     785    1617    1799    1916    2013 
   Size:        120      29     347     168     832     182     117      97

Cuts at:   2013    2042    2405    2876    2908    3520    3598    3877    4488 
   Size:         29     363     471      32     612      78     279     611

Cuts at:   4488    4581    4655    4845    4907    5705     121 
   Size:         93      74     190      62     798    1279

  Fragments arranged by size: 

                1279     832     798     612     611     471     363     347
                 279     190     182     168     120     117      97      93
                  78      74      62      32      29      29
 
BspEI T'CCGG_A

Cuts at:   3093    3093 
   Size:       6863
 
BspHI T'CATG_A

Cuts at:   5295    5295 
   Size:       6863
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    491    1784    2199    3913    4127    4429    4810     491 
   Size:       1293     415    1714     214     302     381    2544

  Fragments arranged by size: 

                2544    1714    1293     415     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     162    1008    1784    2834    5155    5209    6693    6858 
   Size:        112     846     776    1050    2321      54    1484     165

Cuts at:   6858      50 
   Size:         55

  Fragments arranged by size: 

                2321    1484    1050     846     776     165     112      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:   1898    3610    4775    1898 
   Size:       1712    1165    3986

  Fragments arranged by size: 

                3986    1712    1165
 
BsrFI r'CCGG_y

Cuts at:   2638    4861    5042    2638 
   Size:       2223     181    4459

  Fragments arranged by size: 

                4459    2223     181
 
BssHII G'CGCG_C

Cuts at:   3664    3664 
   Size:       6863
 
BssSI C'ACGA_G

Cuts at:    232     691    2043    5134    5842     232 
   Size:        459    1352    3091     708    1253

  Fragments arranged by size: 

                3091    1352    1253     708     459
 
BstAPI GCAn_nnn'nTGC

Cuts at:   4240    4240 
   Size:       6863
 
BstBI TT'CG_AA

Cuts at:   2033    3114    5224    2033 
   Size:       1081    2110    3672

  Fragments arranged by size: 

                3672    2110    1081
 
BstEII G'GTnAC_C

Cuts at:   1152    1152 
   Size:       6863
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1344    2004    2601    1344 
   Size:        660     597    5606

  Fragments arranged by size: 

                5606     660     597
 
BstYI r'GATC_y

Cuts at:      1    1274    2988    2994    3159    3713    3888    4355    4378 
   Size:       1273    1714       6     165     554     175     467      23

Cuts at:   4378    4713    4959    5265    5277    5363    6415       1 
   Size:        335     246     306      12      86    1052     449

  Fragments arranged by size: 

                1714    1273    1052     554     467     449     335     306
                 246     175     165      86      23      12       6
 
Bsu36I CC'TnA_GG

Cuts at:    560    1144    1376    2437     560 
   Size:        584     232    1061    4986

  Fragments arranged by size: 

                4986    1061     584     232
 
BtgI C'CryG_G

Cuts at:    201    1499    2159    2844    3233    3266     201 
   Size:       1298     660     685     389      33    3798

  Fragments arranged by size: 

                3798    1298     685     660     389      33
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    447    2384    3243    4813    4994    5157     447 
   Size:       1937     859    1570     181     163    2153

  Fragments arranged by size: 

                2153    1937    1570     859     181     163
 
BtsI GCAGTG_nn'

Cuts at:    706    1935    4106     706 
   Size:       1229    2171    3463

  Fragments arranged by size: 

                3463    2171    1229
 
DraI TTT'AAA

Cuts at:   3862    3862 
   Size:       6863
 
DraIII CAC_nnn'GTG

Cuts at:   1037    1037 
   Size:       6863
 
DrdI GACnn_nn'nnGTC

Cuts at:    313    4569    5913     313 
   Size:       4256    1344    1263

  Fragments arranged by size: 

                4256    1344    1263
 
EaeI y'GGCC_r

Cuts at:    137     839     848     913    1017    1068    1751    2219    2507 
   Size:        702       9      65     104      51     683     468     288

Cuts at:   2507    2636    2640    3269    4448    4622    5013    5040    6385 
   Size:        129       4     629    1179     174     391      27    1345

Cuts at:   6385     137 
   Size:        615

  Fragments arranged by size: 

                1345    1179     702     683     629     615     468     391
                 288     174     129     104      65      51      27       9
                   4
 
EagI C'GGCC_G

Cuts at:   2640    4448    2640 
   Size:       1808    5055
 
EarI CTCTTCn'nnn_

Cuts at:    464     748     790    1486    1672    2030    2134    2555    3032 
   Size:        284      42     696     186     358     104     421     477

Cuts at:   3032    3757    4886    5096     464 
   Size:        725    1129     210    2231

  Fragments arranged by size: 

                2231    1129     725     696     477     421     358     284
                 210     186     104      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3458    5071    5813    5959    6673    3458 
   Size:       1613     742     146     714    3648

  Fragments arranged by size: 

                3648    1613     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    783    2040    2403    3875     783 
   Size:       1257     363    1472    3771

  Fragments arranged by size: 

                3771    1472    1257     363
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    246     376     553     742     904     958    1164    1273    1495 
   Size:        130     177     189     162      54     206     109     222

Cuts at:   1495    1645    2082    2587    2689    2950    3388    3866    4688 
   Size:        150     437     505     102     261     438     478     822

Cuts at:   4688    5120    5473    6135     246 
   Size:        432     353     662     974

  Fragments arranged by size: 

                 974     822     662     505     478     438     437     432
                 353     261     222     206     189     177     162     150
                 130     109     102      54
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1147    1147 
   Size:       6863
 
EcoO109I rG'GnC_Cy

Cuts at:    504     894    1467    1506    1676    2082    2571    2649    2850 
   Size:        390     573      39     170     406     489      78     201

Cuts at:   2850    3243    4002     504 
   Size:        393     759    3365

  Fragments arranged by size: 

                3365     759     573     489     406     393     390     201
                 170      78      39
 
EcoRI G'AATT_C

Cuts at:   3867    3867 
   Size:       6863
 
EcoRV GAT'ATC

Cuts at:    426     426 
   Size:       6863
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4882    4882 
   Size:       6863
 
FseI GG_CCGG'CC

Cuts at:   2642    2642 
   Size:       6863
 
FspI TGC'GCA

Cuts at:   1442    4644    1442 
   Size:       3202    3661
 
HaeII r_GCGC'y

Cuts at:   1453    2159    4297    4545    5775    6546    1453 
   Size:        706    2138     248    1230     771    1770

  Fragments arranged by size: 

                2138    1770    1230     771     706     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1680    1712    2947    2979    3075    3107    3144    3176    3547 
   Size:         32    1235      32      96      32      37      32     371

Cuts at:   3547    3579    3770    3802    4705    4735    4737    4767    1680 
   Size:         32     191      32     903      30       2      30    3776

  Fragments arranged by size: 

                3776    1235     903     371     191      96      37      32
                  32      32      32      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    645    2172    2313    3684    3902     645 
   Size:       1527     141    1371     218    3606

  Fragments arranged by size: 

                3606    1527    1371     218     141
 
KasI G'GCGC_C

Cuts at:   1449    4293    4541    1449 
   Size:       2844     248    3771

  Fragments arranged by size: 

                3771    2844     248
 
KpnI G_GTAC'C

Cuts at:   3883    3883 
   Size:       6863
 
MluI A'CGCG_T

Cuts at:    715    6296     715 
   Size:       5581    1282
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1109    1367    5622    5806    1109 
   Size:        258    4255     184    2166

  Fragments arranged by size: 

                4255    2166     258     184
 
MscI TGG'CCA

Cuts at:    139     841     850    1070    1753    3271    4624     139 
   Size:        702       9     220     683    1518    1353    2378

  Fragments arranged by size: 

                2378    1518    1353     702     683     220       9
 
MslI CAynn'nnrTG

Cuts at:    760    1999    2197    2281    2348    3565    4852    4979     760 
   Size:       1239     198      84      67    1217    1287     127    2644

  Fragments arranged by size: 

                2644    1287    1239    1217     198     127      84      67
            
 
MspA1I CmG'CkG

Cuts at:    861    2247    3415    3503    3531    3974    4288    4648    5432 
   Size:       1386    1168      88      28     443     314     360     784

Cuts at:   5432    5677     861 
   Size:        245    2047

  Fragments arranged by size: 

                2047    1386    1168     784     443     360     314     245
                  88      28
 
NaeI GCC'GGC

Cuts at:   2640    5044    2640 
   Size:       2404    4459
 
NarI GG'CG_CC

Cuts at:   1450    4294    4542    1450 
   Size:       2844     248    3771

  Fragments arranged by size: 

                3771    2844     248
 
NcoI C'CATG_G

Cuts at:   1499    3233    1499 
   Size:       1734    5129
 
NgoMIV G'CCGG_C

Cuts at:   2638    5042    2638 
   Size:       2404    4459
 
NheI G'CTAG_C

Cuts at:   2605    2605 
   Size:       6863
 
NspI r_CATG'y

Cuts at:   1593    1977    2769    3219    3564    3916    4416    6019    1593 
   Size:        384     792     450     345     352     500    1603    2437

  Fragments arranged by size: 

                2437    1603     792     500     450     384     352     345
            
 
PasI CC'CwG_GG

Cuts at:    492     998    1464    2726    3008    3335     492 
   Size:        506     466    1262     282     327    4020

  Fragments arranged by size: 

                4020    1262     506     466     327     282
 
PciI A'CATG_T

Cuts at:   3215    3560    6015    3215 
   Size:        345    2455    4063

  Fragments arranged by size: 

                4063    2455     345
 
PflMI CCAn_nnn'nTGG

Cuts at:   2496    3486    2496 
   Size:        990    5873
 
PfoI T'CCnGG_A

Cuts at:    881    2225    2699    2777     881 
   Size:       1344     474      78    4967

  Fragments arranged by size: 

                4967    1344     474      78
 
PmeI GTTT'AAAC

Cuts at:   3862    3862 
   Size:       6863
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3799    3831    5309    5341    3799 
   Size:         32    1478      32    5321

  Fragments arranged by size: 

                5321    1478      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    504    1506    2082    2571    2649    2850    3243     504 
   Size:       1002     576     489      78     201     393    4124

  Fragments arranged by size: 

                4124    1002     576     489     393     201      78
 
PshAI GACnn'nnGTC

Cuts at:   1159    1159 
   Size:       6863
 
PstI C_TGCA'G

Cuts at:    188     488     595    2210    2260    2385    3420    3655    3910 
   Size:        300     107    1615      50     125    1035     235     255

Cuts at:   3910    4595     188 
   Size:        685    2456

  Fragments arranged by size: 

                2456    1615    1035     685     300     255     235     125
                 107      50
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6863
 
PvuII CAG'CTG

Cuts at:    861    2247    3415    3531    4288    4648     861 
   Size:       1386    1168     116     757     360    3076

  Fragments arranged by size: 

                3076    1386    1168     757     360     116
 
RsrII CG'GwC_CG

Cuts at:   5058    5058 
   Size:       6863
 
SacI G_AGCT'C

Cuts at:    785    2042    2405    3877     785 
   Size:       1257     363    1472    3771

  Fragments arranged by size: 

                3771    1472    1257     363
 
SalI G'TCGA_C

Cuts at:   2170    3682    3900    2170 
   Size:       1512     218    5133

  Fragments arranged by size: 

                5133    1512     218
 
SanDI GG'GwC_CC

Cuts at:   2649    2649 
   Size:       6863
 
SapI GCTCTTCn'nnn_

Cuts at:    748     790    1486    2030    2555    3032    4886    5096     748 
   Size:         42     696     544     525     477    1854     210    2515

  Fragments arranged by size: 

                2515    1854     696     544     525     477     210      42
            
 
SbfI CC_TGCA'GG

Cuts at:   2210    3910    2210 
   Size:       1700    5163
 
ScaI AGT'ACT

Cuts at:   2614    6380    2614 
   Size:       3766    3097
 
SfcI C'TryA_G

Cuts at:     33     184     484     591    2206    2256    2381    3416    3651 
   Size:        151     300     107    1615      50     125    1035     235

Cuts at:   3651    3906    4591    5559    5750      33 
   Size:        255     685     968     191    1146

  Fragments arranged by size: 

                1615    1146    1035     968     685     300     255     235
                 191     151     125     107      50
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    847     912     847 
   Size:         65    6798
 
SfoI GGC'GCC

Cuts at:   1451    4295    4543    1451 
   Size:       2844     248    3771

  Fragments arranged by size: 

                3771    2844     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6863
 
SmaI CCC'GGG

Cuts at:    510    1704    3885     510 
   Size:       1194    2181    3488

  Fragments arranged by size: 

                3488    2181    1194
 
SmlI C'TyrA_G

Cuts at:   2866    3017    3781    4018    4933    5647    5909    2866 
   Size:        151     764     237     915     714     262    3820

  Fragments arranged by size: 

                3820     915     764     714     262     237     151
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6863
 
SphI G_CATG'C

Cuts at:   1977    3916    4416    1977 
   Size:       1939     500    4424

  Fragments arranged by size: 

                4424    1939     500
 
StuI AGG'CCT

Cuts at:   1942    3649    1942 
   Size:       1707    5156
 
StyI C'CwwG_G

Cuts at:    901    1499    1509    1517    1526    2022    2495    3125    3233 
   Size:        598      10       8       9     496     473     630     108

Cuts at:   3233    3997    4351     901 
   Size:        764     354    3413

  Fragments arranged by size: 

                3413     764     630     598     496     473     354     108
                  10       9       8
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    141     693    4446    4579    5038    5247     141 
   Size:        552    3753     133     459     209    1757

  Fragments arranged by size: 

                3753    1757     552     459     209     133
 
TatI w'GTAC_w

Cuts at:   1742    2612    6378    1742 
   Size:        870    3766    2227

  Fragments arranged by size: 

                3766    2227     870
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1323    1355    2000    2032    6380    6412    1323 
   Size:         32     645      32    4348      32    1774

  Fragments arranged by size: 

                4348    1774     645      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    399    3735    4660     399 
   Size:       3336     925    2602

  Fragments arranged by size: 

                3336    2602     925
 
XbaI T'CTAG_A

Cuts at:   3894    3894 
   Size:       6863
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    270    2283    2517    2562    3264    3841    4358     270 
   Size:       2013     234      45     702     577     517    2775

  Fragments arranged by size: 

                2775    2013     702     577     517     234      45
 
XmaI C'CCGG_G

Cuts at:    508    1702    3883     508 
   Size:       1194    2181    3488

  Fragments arranged by size: 

                3488    2181    1194
 
XmnI GAAnn'nnTTC

Cuts at:   1480    3307    1480 
   Size:       1827    5036
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     ApaI     AscI     BaeI    BfrBI
    BsiWI     BsmI    BsrGI  BstZ17I     ClaI    CspCI    FspAI  HindIII
     HpaI     MfeI     NdeI     NotI     NruI     NsiI     PacI     PmlI
     PsiI   PspOMI    PspXI     PsrI    SacII    SexAI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI     ZraI
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