Restriction map for pF1KA0676
 (Linear) (Six-base) MAPSORT of: KIAA0676.seq  Check: 5647  from: 1  to: 3750



KIAA0676.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2091    3750 
   Size:       2091    1659
 
AccI GT'mk_AC

Cuts at:      0     209    2063    3575    3750 
   Size:        209    1854    1512     175

  Fragments arranged by size: 

                1854    1512     209     175
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     850    1387    1537    3280    3750 
   Size:        445     405     537     150    1743     470

  Fragments arranged by size: 

                1743     537     470     445     405     150
 
AflIII A'CryG_T

Cuts at:      0     607    3107    3452    3750 
   Size:        607    2500     345     298

  Fragments arranged by size: 

                2500     607     345     298
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3453    3750 
   Size:       3453     297
 
AleI CACnn'nnGTG

Cuts at:      0    1891    2240    3750 
   Size:       1891     349    1510

  Fragments arranged by size: 

                1891    1510     349
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2258    3750 
   Size:       2258    1492
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3691    3723    3750 
   Size:       3691      32      27

  Fragments arranged by size: 

                3691      32      27
 
AlwNI CAG_nnn'CTG

Cuts at:      0     291     491     756    1362    1977    2142    2370    3138 
   Size:        291     200     265     606     615     165     228     768

Cuts at:   3138    3233    3426    3609    3750 
   Size:         95     193     183     141

  Fragments arranged by size: 

                 768     615     606     291     265     228     200     193
                 183     165     141      95
 
ApoI r'AATT_y

Cuts at:      0    2317    3750 
   Size:       2317    1433
 
AvaI C'yCGr_G

Cuts at:      0     400     640     848    1594    1684    3750 
   Size:        400     240     208     746      90    2066

  Fragments arranged by size: 

                2066     746     400     240     208      90
 
AvrII C'CTAG_G

Cuts at:      0     793    3750 
   Size:        793    2957
 
BanI G'GyrC_C

Cuts at:      0     130    1341    3568    3750 
   Size:        130    1211    2227     182

  Fragments arranged by size: 

                2227    1211     182     130
 
BanII G_rGCy'C

Cuts at:      0      13     677    1905    1934    2297    2800    3412    3490 
   Size:         13     664    1228      29     363     503     612      78

Cuts at:   3490    3750 
   Size:        260

  Fragments arranged by size: 

                1228     664     612     503     363     260      78      29
                  13
 
BbeI G_GCGC'C

Cuts at:      0    1345    3750 
   Size:       1345    2405
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     112    1247    2345    3125    3509    3750 
   Size:        112    1135    1098     780     384     241

  Fragments arranged by size: 

                1135    1098     780     384     241     112
 
BbvCI CC'TCA_GC

Cuts at:      0    1309    2026    3750 
   Size:       1309     717    1724

  Fragments arranged by size: 

                1724    1309     717
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1675    1709    2986    3020    3750 
   Size:       1675      34    1277      34     730

  Fragments arranged by size: 

                1675    1277     730      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1534    3750 
   Size:       1534    2216
 
BclI T'GATC_A

Cuts at:      0    2693    3750 
   Size:       2693    1057
 
BglI GCCn_nnn'nGGC

Cuts at:      0     166     739     804    1414    2824    3160    3750 
   Size:        166     573      65     610    1410     336     590

  Fragments arranged by size: 

                1410     610     590     573     336     166      65
 
BglII A'GATC_T

Cuts at:      0    2880    3750 
   Size:       2880     870
 
BlpI GC'TnA_GC

Cuts at:      0       7     419    1607    2251    3750 
   Size:          7     412    1188     644    1499

  Fragments arranged by size: 

                1499    1188     644     412       7
 
Bme1580I G_kGCm'C

Cuts at:      0     133     162     509    1509    3750 
   Size:        133      29     347    1000    2241

  Fragments arranged by size: 

                2241    1000     347     133      29
 
BmgBI CAC'GTC

Cuts at:      0     141    3750 
   Size:        141    3609
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     267    2517    3750 
   Size:        267    2250    1233

  Fragments arranged by size: 

                2250    1233     267
 
BmtI G_CTAG'C

Cuts at:      0    2501    3750 
   Size:       2501    1249
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2237    2868    3750 
   Size:       2237     631     882

  Fragments arranged by size: 

                2237     882     631
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     268     634     796    1056    1165    1974    2479 
   Size:        138     130     366     162     260     109     809     505

Cuts at:   2479    2581    2842    3750 
   Size:        102     261     908

  Fragments arranged by size: 

                 908     809     505     366     261     260     162     138
                 130     109     102
 
Bpu10I CC'TnA_GC

Cuts at:      0    1309    2026    3750 
   Size:       1309     717    1724

  Fragments arranged by size: 

                1724    1309     717
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2743    2894    3658    3750 
   Size:       2743     151     764      92

  Fragments arranged by size: 

                2743     764     151      92
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     700     830    1658    3308    3750 
   Size:        700     130     828    1650     442

  Fragments arranged by size: 

                1650     828     700     442     130
 
BsaBI GATnn'nnATC

Cuts at:      0     970    2885    3750 
   Size:        970    1915     865

  Fragments arranged by size: 

                1915     970     865
 
BsaHI Gr'CG_yC

Cuts at:      0      84     845     934    1342    2123    3750 
   Size:         84     761      89     408     781    1627

  Fragments arranged by size: 

                1627     781     761     408      89      84
 
BsaWI w'CCGG_w

Cuts at:      0    2570    2985    3750 
   Size:       2570     415     765

  Fragments arranged by size: 

                2570     765     415
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1892    1922    2967    2997    3691    3721    3750 
   Size:       1892      30    1045      30     694      30      29

  Fragments arranged by size: 

                1892    1045     694      30      30      30      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31    1021    1256    1450    1546    1666    1840    1843 
   Size:         31     990     235     194      96     120     174       3

Cuts at:   1843    2213    2421    2844    2858    2968    2975    2980    3259 
   Size:        370     208     423      14     110       7       5     279

Cuts at:   3259    3750 
   Size:        491

  Fragments arranged by size: 

                 990     491     423     370     279     235     208     194
                 174     120     110      96      31      14       7       5
                   3
 
BseYI C'CCAG_C

Cuts at:      0     564    1084    1260    1296    1710    2142    2178    2545 
   Size:        564     520     176      36     414     432      36     367

Cuts at:   2545    2787    3303    3419    3750 
   Size:        242     516     116     331

  Fragments arranged by size: 

                 564     520     516     432     414     367     331     242
                 176     116      36      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     610     906     934    1180    1654    2535    3750 
   Size:        169     441     296      28     246     474     881    1215

  Fragments arranged by size: 

                1215     881     474     441     296     246     169      28
            
 
BsiEI CG_ry'CG

Cuts at:      0    2535    3750 
   Size:       2535    1215
 
BsiHKAI G_wGCw'C

Cuts at:      0     677    1691    1808    1934    2297    2768    3750 
   Size:        677    1014     117     126     363     471     982

  Fragments arranged by size: 

                1014     982     677     471     363     126     117
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     383    3034    3750 
   Size:        383    2651     716

  Fragments arranged by size: 

                2651     716     383
 
Bsp1286I G_dGCh'C

Cuts at:      0      13     133     162     509     677    1509    1691    1808 
   Size:         13     120      29     347     168     832     182     117

Cuts at:   1808    1905    1934    2297    2768    2800    3412    3490    3750 
   Size:         97      29     363     471      32     612      78     260

  Fragments arranged by size: 

                 832     612     471     363     347     260     182     168
                 120     117      97      78      32      29      29      13
            
 
BspEI T'CCGG_A

Cuts at:      0    2985    3750 
   Size:       2985     765
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     383    1676    2091    3750 
   Size:        383    1293     415    1659

  Fragments arranged by size: 

                1659    1293     415     383
 
BsrBI CCG'CTC

Cuts at:      0      54     900    1676    2726    3750 
   Size:         54     846     776    1050    1024

  Fragments arranged by size: 

                1050    1024     846     776      54
 
BsrDI GCAATG_nn'

Cuts at:      0    1790    3502    3750 
   Size:       1790    1712     248

  Fragments arranged by size: 

                1790    1712     248
 
BsrFI r'CCGG_y

Cuts at:      0    2530    3750 
   Size:       2530    1220
 
BssHII G'CGCG_C

Cuts at:      0    3556    3750 
   Size:       3556     194
 
BssSI C'ACGA_G

Cuts at:      0     124     583    1935    3750 
   Size:        124     459    1352    1815

  Fragments arranged by size: 

                1815    1352     459     124
 
BstBI TT'CG_AA

Cuts at:      0    1925    3006    3750 
   Size:       1925    1081     744

  Fragments arranged by size: 

                1925    1081     744
 
BstEII G'GTnAC_C

Cuts at:      0    1044    3750 
   Size:       1044    2706
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1236    1896    2493    3750 
   Size:       1236     660     597    1257

  Fragments arranged by size: 

                1257    1236     660     597
 
BstYI r'GATC_y

Cuts at:      0    1166    2880    2886    3051    3605    3750 
   Size:       1166    1714       6     165     554     145

  Fragments arranged by size: 

                1714    1166     554     165     145       6
 
Bsu36I CC'TnA_GG

Cuts at:      0     452    1036    1268    2329    3750 
   Size:        452     584     232    1061    1421

  Fragments arranged by size: 

                1421    1061     584     452     232
 
BtgI C'CryG_G

Cuts at:      0      93    1391    2051    2736    3125    3158    3750 
   Size:         93    1298     660     685     389      33     592

  Fragments arranged by size: 

                1298     685     660     592     389      93      33
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     339    2276    3135    3750 
   Size:        339    1937     859     615

  Fragments arranged by size: 

                1937     859     615     339
 
BtsI GCAGTG_nn'

Cuts at:      0     598    1827    3750 
   Size:        598    1229    1923

  Fragments arranged by size: 

                1923    1229     598
 
DraIII CAC_nnn'GTG

Cuts at:      0     929    3750 
   Size:        929    2821
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     205    3750 
   Size:        205    3545
 
EaeI y'GGCC_r

Cuts at:      0      29     731     740     805     909     960    1643    2111 
   Size:         29     702       9      65     104      51     683     468

Cuts at:   2111    2399    2528    2532    3161    3750 
   Size:        288     129       4     629     589

  Fragments arranged by size: 

                 702     683     629     589     468     288     129     104
                  65      51      29       9       4
 
EagI C'GGCC_G

Cuts at:      0    2532    3750 
   Size:       2532    1218
 
EarI CTCTTCn'nnn_

Cuts at:      0     356     640     682    1378    1564    1922    2026    2447 
   Size:        356     284      42     696     186     358     104     421

Cuts at:   2447    2924    3649    3750 
   Size:        477     725     101

  Fragments arranged by size: 

                 725     696     477     421     358     356     284     186
                 104     101      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3350    3750 
   Size:       3350     400
 
EcoICRI GAG'CTC

Cuts at:      0     675    1932    2295    3750 
   Size:        675    1257     363    1455

  Fragments arranged by size: 

                1455    1257     675     363
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     268     445     634     796     850    1056    1165 
   Size:        138     130     177     189     162      54     206     109

Cuts at:   1165    1387    1537    1974    2479    2581    2842    3280    3750 
   Size:        222     150     437     505     102     261     438     470

  Fragments arranged by size: 

                 505     470     438     437     261     222     206     189
                 177     162     150     138     130     109     102      54
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1039    3750 
   Size:       1039    2711
 
EcoO109I rG'GnC_Cy

Cuts at:      0     396     786    1359    1398    1568    1974    2463    2541 
   Size:        396     390     573      39     170     406     489      78

Cuts at:   2541    2742    3135    3750 
   Size:        201     393     615

  Fragments arranged by size: 

                 615     573     489     406     396     393     390     201
                 170      78      39
 
EcoRV GAT'ATC

Cuts at:      0     318    3750 
   Size:        318    3432
 
FseI GG_CCGG'CC

Cuts at:      0    2534    3750 
   Size:       2534    1216
 
FspI TGC'GCA

Cuts at:      0    1334    3750 
   Size:       1334    2416
 
HaeII r_GCGC'y

Cuts at:      0    1345    2051    3750 
   Size:       1345     706    1699

  Fragments arranged by size: 

                1699    1345     706
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1572    1604    2839    2871    2967    2999    3036    3068 
   Size:       1572      32    1235      32      96      32      37      32

Cuts at:   3068    3439    3471    3662    3694    3750 
   Size:        371      32     191      32      56

  Fragments arranged by size: 

                1572    1235     371     191      96      56      37      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     537    2064    2205    3576    3750 
   Size:        537    1527     141    1371     174

  Fragments arranged by size: 

                1527    1371     537     174     141
 
KasI G'GCGC_C

Cuts at:      0    1341    3750 
   Size:       1341    2409
 
MluI A'CGCG_T

Cuts at:      0     607    3750 
   Size:        607    3143
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1001    1259    3750 
   Size:       1001     258    2491

  Fragments arranged by size: 

                2491    1001     258
 
MscI TGG'CCA

Cuts at:      0      31     733     742     962    1645    3163    3750 
   Size:         31     702       9     220     683    1518     587

  Fragments arranged by size: 

                1518     702     683     587     220      31       9
 
MslI CAynn'nnrTG

Cuts at:      0     652    1891    2089    2173    2240    3457    3750 
   Size:        652    1239     198      84      67    1217     293

  Fragments arranged by size: 

                1239    1217     652     293     198      84      67
 
MspA1I CmG'CkG

Cuts at:      0     753    2139    3307    3395    3423    3750 
   Size:        753    1386    1168      88      28     327

  Fragments arranged by size: 

                1386    1168     753     327      88      28
 
NaeI GCC'GGC

Cuts at:      0    2532    3750 
   Size:       2532    1218
 
NarI GG'CG_CC

Cuts at:      0    1342    3750 
   Size:       1342    2408
 
NcoI C'CATG_G

Cuts at:      0    1391    3125    3750 
   Size:       1391    1734     625

  Fragments arranged by size: 

                1734    1391     625
 
NgoMIV G'CCGG_C

Cuts at:      0    2530    3750 
   Size:       2530    1220
 
NheI G'CTAG_C

Cuts at:      0    2497    3750 
   Size:       2497    1253
 
NspI r_CATG'y

Cuts at:      0    1485    1869    2661    3111    3456    3750 
   Size:       1485     384     792     450     345     294

  Fragments arranged by size: 

                1485     792     450     384     345     294
 
PasI CC'CwG_GG

Cuts at:      0     384     890    1356    2618    2900    3227    3750 
   Size:        384     506     466    1262     282     327     523

  Fragments arranged by size: 

                1262     523     506     466     384     327     282
 
PciI A'CATG_T

Cuts at:      0    3107    3452    3750 
   Size:       3107     345     298

  Fragments arranged by size: 

                3107     345     298
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2388    3378    3750 
   Size:       2388     990     372

  Fragments arranged by size: 

                2388     990     372
 
PfoI T'CCnGG_A

Cuts at:      0     773    2117    2591    2669    3750 
   Size:        773    1344     474      78    1081

  Fragments arranged by size: 

                1344    1081     773     474      78
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3691    3723    3750 
   Size:       3691      32      27

  Fragments arranged by size: 

                3691      32      27
 
PpuMI rG'GwC_Cy

Cuts at:      0     396    1398    1974    2463    2541    2742    3135    3750 
   Size:        396    1002     576     489      78     201     393     615

  Fragments arranged by size: 

                1002     615     576     489     396     393     201      78
            
 
PshAI GACnn'nnGTC

Cuts at:      0    1051    3750 
   Size:       1051    2699
 
PstI C_TGCA'G

Cuts at:      0      80     380     487    2102    2152    2277    3312    3547 
   Size:         80     300     107    1615      50     125    1035     235

Cuts at:   3547    3750 
   Size:        203

  Fragments arranged by size: 

                1615    1035     300     235     203     125     107      80
                  50
 
PvuII CAG'CTG

Cuts at:      0     753    2139    3307    3423    3750 
   Size:        753    1386    1168     116     327

  Fragments arranged by size: 

                1386    1168     753     327     116
 
SacI G_AGCT'C

Cuts at:      0     677    1934    2297    3750 
   Size:        677    1257     363    1453

  Fragments arranged by size: 

                1453    1257     677     363
 
SalI G'TCGA_C

Cuts at:      0    2062    3574    3750 
   Size:       2062    1512     176

  Fragments arranged by size: 

                2062    1512     176
 
SanDI GG'GwC_CC

Cuts at:      0    2541    3750 
   Size:       2541    1209
 
SapI GCTCTTCn'nnn_

Cuts at:      0     640     682    1378    1922    2447    2924    3750 
   Size:        640      42     696     544     525     477     826

  Fragments arranged by size: 

                 826     696     640     544     525     477      42
 
SbfI CC_TGCA'GG

Cuts at:      0    2102    3750 
   Size:       2102    1648
 
ScaI AGT'ACT

Cuts at:      0    2506    3750 
   Size:       2506    1244
 
SfcI C'TryA_G

Cuts at:      0      76     376     483    2098    2148    2273    3308    3543 
   Size:         76     300     107    1615      50     125    1035     235

Cuts at:   3543    3750 
   Size:        207

  Fragments arranged by size: 

                1615    1035     300     235     207     125     107      76
                  50
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     739     804    3750 
   Size:        739      65    2946

  Fragments arranged by size: 

                2946     739      65
 
SfoI GGC'GCC

Cuts at:      0    1343    3750 
   Size:       1343    2407
 
SmaI CCC'GGG

Cuts at:      0     402    1596    3750 
   Size:        402    1194    2154

  Fragments arranged by size: 

                2154    1194     402
 
SmlI C'TyrA_G

Cuts at:      0    2758    2909    3673    3750 
   Size:       2758     151     764      77

  Fragments arranged by size: 

                2758     764     151      77
 
SphI G_CATG'C

Cuts at:      0    1869    3750 
   Size:       1869    1881
 
StuI AGG'CCT

Cuts at:      0    1834    3541    3750 
   Size:       1834    1707     209

  Fragments arranged by size: 

                1834    1707     209
 
StyI C'CwwG_G

Cuts at:      0     793    1391    1401    1409    1418    1914    2387    3017 
   Size:        793     598      10       8       9     496     473     630

Cuts at:   3017    3125    3750 
   Size:        108     625

  Fragments arranged by size: 

                 793     630     625     598     496     473     108      10
                   9       8
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      33     585    3750 
   Size:         33     552    3165

  Fragments arranged by size: 

                3165     552      33
 
TatI w'GTAC_w

Cuts at:      0    1634    2504    3750 
   Size:       1634     870    1246

  Fragments arranged by size: 

                1634    1246     870
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1215    1247    1892    1924    3750 
   Size:       1215      32     645      32    1826

  Fragments arranged by size: 

                1826    1215     645      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     291    3627    3750 
   Size:        291    3336     123

  Fragments arranged by size: 

                3336     291     123
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     162    2175    2409    2454    3156    3733    3750 
   Size:        162    2013     234      45     702     577      17

  Fragments arranged by size: 

                2013     702     577     234     162      45      17
 
XmaI C'CCGG_G

Cuts at:      0     400    1594    3750 
   Size:        400    1194    2156

  Fragments arranged by size: 

                2156    1194     400
 
XmnI GAAnn'nnTTC

Cuts at:      0    1372    3199    3750 
   Size:       1372    1827     551

  Fragments arranged by size: 

                1827    1372     551
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AhdI     AleI     AlfI     AloI
    AlwNI     ApoI     AvaI    AvrII     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FseI     FspI    HaeII
    Hin4I   HincII     KasI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NspI     PasI     PciI
    PflMI     PfoI     PpiI    PpuMI    PshAI     PstI    PvuII     SacI
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     ApaI    ApaLI
     AscI     AseI    AsiSI     BaeI    BamHI    BfrBI    BsaAI    BsiWI
     BsmI    BspHI    BsrGI   BstAPI  BstZ17I     ClaI    CspCI     DraI
    EcoRI     FalI    FspAI  HindIII     HpaI     KpnI     MfeI     NdeI
     NotI     NruI     NsiI     PacI     PmeI     PmlI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII    SexAI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     XhoI     ZraI
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