Restriction map for pF1KA0586
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AgeI        AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AspI        
AviII       AvrII       BciVI       BcnI        BfrI        
BglI        BlnI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspEI       BsrBI       
BsrGI       BssHII      BssSI       BstBI       BstEII      
BstPI       BstUI       Bsu36I      CpoI        DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FauI        FseI        
FspI        HgaI        KpnI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NciI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SbfI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    SspBI       StuI        SwaI        Tth111I     
XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          318  GTATAC               GT^[AC][GT]AC
AcyI         2917  GGCGCC               G[GA]^CG[CT]C
AhdI         4570  GACATGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3108  GTGCAC               G^TGCAC
ApaLI        3108  GTGCAC               G^TGCAC
AspEI        4570  GACATGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         4525  CTCGAG               C^[CT]CG[GA]G
BalI         1281  TGGCCA               TGG^CCA
BamHI        2373  GGATCC               G^GATCC
BanII        2815  GGGCTC               G[GA]GC[CT]^C
BbeI         2917  GGCGCC               GGCGC^C
BbrPI         149  CACGTG               CAC^GTG
BbvCI        2380  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2985  CGAAGCTCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         3532  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3532  GCTCAGC              GC^T[ACGT]AGC
BsaHI        2917  GGCGCC               G[GA]^CG[CT]C
BsmI          644  GCATTC               GAATGC(1/-1)
BsoBI        4525  CTCGAG               C^[CT]CG[GA]G
BspDI        2575  ATCGAT               AT^CGAT
BspLU11I     4435  ACATGT               A^CATGT
Bst1107I      318  GTATAC               GTA^TAC
BstXI         824  CCAGAGCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       318  GTATAC               GTA^TAC
CelII        3532  GCTCAGC              GC^T[ACGT]AGC
CfoI         2918  GCGC                 GCG^C
ClaI         2575  ATCGAT               AT^CGAT
EaeI         1281  TGGCCA               [CT]^GGCC[GA]
Eam1105I     4570  GACATGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        3826  CCTAGTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1362  GAATTC               G^AATTC
HaeII        2917  GGCGCC               [GA]GCGC^[CT]
HhaI         2918  GCGC                 GCG^C
Hin1I        2917  GGCGCC               G[GA]^CG[CT]C
HinP1I       2918  GCGC                 G^CGC
HpaI         2980  GTTAAC               GTT^AAC
KasI         2917  GGCGCC               G^GCGCC
MluNI        1281  TGGCCA               TGG^CCA
MscI         1281  TGGCCA               TGG^CCA
NarI         2917  GGCGCC               GG^CGCC
PaeR7I       4525  CTCGAG               C^TCGAG
PmaCI         149  CACGTG               CAC^GTG
PmlI          149  CACGTG               CAC^GTG
PpuMI        3042  AGGTCCT              [GA]G^G[AT]CC[CT]
ScaI         1452  AGTACT               AGT^ACT
SexAI        4389  ACCAGGT              A^CC[AT]GGT
SfoI         2917  GGCGCC               GGC^GCC
SspI         2126  AATATT               AAT^ATT
XhoI         4525  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AspHI        3108  GTGCAC               G[AT]GC[AT]^C
             3636  GAGCAC              
BanI         2917  GGCGCC               G^G[CT][GA]CC
             3580  GGCACC              
BbsI          110  GTCTTC               GAAGAC(2/6)
             4578  GTCTTC              
BclI          678  TGATCA               T^GATCA
             1650  TGATCA              
BglII        1350  AGATCT               A^GATCT
             1396  AGATCT              
BpuAI         110  GTCTTC               GAAGAC(2/6)
             4578  GTCTTC              
BsaAI         149  CACGTG               [CT]AC^GT[GA]
             4340  TACGTG              
BsaBI        3208  GATTCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4396  GATATGGATC          
BsiHKAI      3108  GTGCAC               G[AT]GC[AT]^C
             3636  GAGCAC              
BsrDI         513  CATTGC               GCAATG(2/0)
             3003  CATTGC              
BsrFI        3096  GCCGGC               [GA]^CCGG[CT]
             3577  GCCGGC              
Cfr10I       3096  GCCGGC               [GA]^CCGG[CT]
             3577  GCCGGC              
DraI          354  TTTAAA               TTT^AAA
              921  TTTAAA              
DraII        3042  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             3587  AGGCCCC             
DraIII        461  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4388  CACCAGGTG           
EcoO109I     3042  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             3587  AGGCCCC             
FbaI          678  TGATCA               T^GATCA
             1650  TGATCA              
HincII       1726  GTCAAC               GT[CT]^[GA]AC
             2980  GTTAAC              
HindII       1726  GTCAAC               GT[CT]^[GA]AC
             2980  GTTAAC              
HindIII      1342  AAGCTT               A^AGCTT
             1372  AAGCTT              
MamI         3208  GATTCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4396  GATATGGATC          
NaeI         3096  GCCGGC               GCC^GGC
             3577  GCCGGC              
NcoI          209  CCATGG               C^CATGG
             3406  CCATGG              
NgoMIV       3096  GCCGGC               G^CCGGC
             3577  GCCGGC              
PflMI        2239  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3401  CCAAGCCATGG         
PstI          494  CTGCAG               CTGCA^G
             1598  CTGCAG              
SapI         1222  GAAGAGC              GCTCTTC(1/4)
             1771  GAAGAGC             
SpeI          130  ACTAGT               A^CTAGT
              790  ACTAGT              
SphI          374  GCATGC               GCATG^C
             1802  GCATGC              
Van91I       2239  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3401  CCAAGCCATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AvaII       
BbvI        BfaI        BmyI        BpmI        BsaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BspMI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DsaI        EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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