Restriction map for pF1KA0586
 (Linear) (Six-base) MAPSORT of: KIAA0586.seq  Check: 4208  from: 1  to: 4599



KIAA0586.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     623    2250    3168    4599 
   Size:        623    1627     918    1431

  Fragments arranged by size: 

                1627    1431     918     623
 
AccI GT'mk_AC

Cuts at:      0     319    4599 
   Size:        319    4280
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3710    4283    4599 
   Size:       3710     573     316

  Fragments arranged by size: 

                3710     573     316
 
AflIII A'CryG_T

Cuts at:      0     621    4341    4435    4599 
   Size:        621    3720      94     164

  Fragments arranged by size: 

                3720     621     164      94
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4575    4599 
   Size:       4575      24
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2855    3123    4599 
   Size:       2855     268    1476

  Fragments arranged by size: 

                2855    1476     268
 
AlwNI CAG_nnn'CTG

Cuts at:      0     502    2620    3616    4545    4599 
   Size:        502    2118     996     929      54

  Fragments arranged by size: 

                2118     996     929     502      54
 
ApaLI G'TGCA_C

Cuts at:      0    3108    4599 
   Size:       3108    1491
 
ApoI r'AATT_y

Cuts at:      0     455    1106    1362    1604    2609    3862    4191    4599 
   Size:        455     651     256     242    1005    1253     329     408

  Fragments arranged by size: 

                1253    1005     651     455     408     329     256     242
            
 
AvaI C'yCGr_G

Cuts at:      0    4525    4599 
   Size:       4525      74
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     391     424    4599 
   Size:        391      33    4175

  Fragments arranged by size: 

                4175     391      33
 
BamHI G'GATC_C

Cuts at:      0    2373    4599 
   Size:       2373    2226
 
BanI G'GyrC_C

Cuts at:      0    2917    3580    4599 
   Size:       2917     663    1019

  Fragments arranged by size: 

                2917    1019     663
 
BanII G_rGCy'C

Cuts at:      0    2819    4599 
   Size:       2819    1780
 
BbeI G_GCGC'C

Cuts at:      0    2921    4599 
   Size:       2921    1678
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     103    4571    4599 
   Size:        103    4468      28

  Fragments arranged by size: 

                4468     103      28
 
BbvCI CC'TCA_GC

Cuts at:      0    2381    4599 
   Size:       2381    2218
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2974    3008    4599 
   Size:       2974      34    1591

  Fragments arranged by size: 

                2974    1591      34
 
BclI T'GATC_A

Cuts at:      0     678    1650    4599 
   Size:        678     972    2949

  Fragments arranged by size: 

                2949     972     678
 
BglII A'GATC_T

Cuts at:      0    1350    1396    4599 
   Size:       1350      46    3203

  Fragments arranged by size: 

                3203    1350      46
 
BlpI GC'TnA_GC

Cuts at:      0    3533    4599 
   Size:       3533    1066
 
Bme1580I G_kGCm'C

Cuts at:      0    3112    4599 
   Size:       3112    1487
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     458    2399    4269    4599 
   Size:        458    1941    1870     330

  Fragments arranged by size: 

                1941    1870     458     330
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4551    4599 
   Size:       4551      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     807    2267    2342    2495    2653    2908    3173    3308 
   Size:        807    1460      75     153     158     255     265     135

Cuts at:   3308    3530    3652    4599 
   Size:        222     122     947

  Fragments arranged by size: 

                1460     947     807     265     255     222     158     153
                 135     122      75
 
Bpu10I CC'TnA_GC

Cuts at:      0    2381    3164    3497    4599 
   Size:       2381     783     333    1102

  Fragments arranged by size: 

                2381    1102     783     333
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     769    3440    4599 
   Size:        769    2671    1159

  Fragments arranged by size: 

                2671    1159     769
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1980    2992    3413    4599 
   Size:       1980    1012     421    1186

  Fragments arranged by size: 

                1980    1186    1012     421
 
BsaAI yAC'GTr

Cuts at:      0     151    4342    4599 
   Size:        151    4191     257

  Fragments arranged by size: 

                4191     257     151
 
BsaBI GATnn'nnATC

Cuts at:      0    3212    4400    4599 
   Size:       3212    1188     199

  Fragments arranged by size: 

                3212    1188     199
 
BsaHI Gr'CG_yC

Cuts at:      0    2918    4599 
   Size:       2918    1681
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     510     540    2240    2270    3303    3306    3333    3336 
   Size:        510      30    1700      30    1033       3      27       3

Cuts at:   3336    4599 
   Size:       1263

  Fragments arranged by size: 

                1700    1263    1033     510      30      30      27       3
                   3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     810    1445    2369    2471    2474    3311    4599 
   Size:        810     635     924     102       3     837    1288

  Fragments arranged by size: 

                1288     924     837     810     635     102       3
 
BseYI C'CCAG_C

Cuts at:      0    3015    3920    3989    4599 
   Size:       3015     905      69     610

  Fragments arranged by size: 

                3015     905     610      69
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     562     764    2413    4599 
   Size:        562     202    1649    2186

  Fragments arranged by size: 

                2186    1649     562     202
 
BsiHKAI G_wGCw'C

Cuts at:      0    3112    3640    4599 
   Size:       3112     528     959

  Fragments arranged by size: 

                3112     959     528
 
BsmI GAATG_Cn'

Cuts at:      0     644    4599 
   Size:        644    3955
 
Bsp1286I G_dGCh'C

Cuts at:      0    2819    3112    3640    4599 
   Size:       2819     293     528     959

  Fragments arranged by size: 

                2819     959     528     293
 
BspHI T'CATG_A

Cuts at:      0     675    1272    3216    4599 
   Size:        675     597    1944    1383

  Fragments arranged by size: 

                1944    1383     675     597
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     623    2250    2385    3168    3431    4599 
   Size:        623    1627     135     783     263    1168

  Fragments arranged by size: 

                1627    1168     783     623     263     135
 
BsrDI GCAATG_nn'

Cuts at:      0     512    3002    4599 
   Size:        512    2490    1597

  Fragments arranged by size: 

                2490    1597     512
 
BsrFI r'CCGG_y

Cuts at:      0    3096    3577    4599 
   Size:       3096     481    1022

  Fragments arranged by size: 

                3096    1022     481
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      97     255     502     505    4599 
   Size:         97     158     247       3    4094

  Fragments arranged by size: 

                4094     247     158      97       3
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     831    4599 
   Size:        831    3768
 
BstYI r'GATC_y

Cuts at:      0    1350    1396    2373    4599 
   Size:       1350      46     977    2226

  Fragments arranged by size: 

                2226    1350     977      46
 
BstZ17I GTA'TAC

Cuts at:      0     320    4599 
   Size:        320    4279
 
BtgI C'CryG_G

Cuts at:      0     209     488    3406    4599 
   Size:        209     279    2918    1193

  Fragments arranged by size: 

                2918    1193     279     209
 
BtsI GCAGTG_nn'

Cuts at:      0     251     836    2714    2919    4466    4599 
   Size:        251     585    1878     205    1547     133

  Fragments arranged by size: 

                1878    1547     585     251     205     133
 
ClaI AT'CG_AT

Cuts at:      0    2576    4599 
   Size:       2576    2023
 
DraI TTT'AAA

Cuts at:      0     356     923    4599 
   Size:        356     567    3676

  Fragments arranged by size: 

                3676     567     356
 
DraIII CAC_nnn'GTG

Cuts at:      0     466    4393    4599 
   Size:        466    3927     206

  Fragments arranged by size: 

                3927     466     206
 
EaeI y'GGCC_r

Cuts at:      0    1281    4599 
   Size:       1281    3318
 
EarI CTCTTCn'nnn_

Cuts at:      0       4     269    1217    1766    2531    3431    4599 
   Size:          4     265     948     549     765     900    1168

  Fragments arranged by size: 

                1168     948     900     765     549     265       4
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2678    4599 
   Size:       2678    1921
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     807    2267    2342    2495    2653    2908    3173    3308 
   Size:        807    1460      75     153     158     255     265     135

Cuts at:   3308    3530    3652    3710    4283    4599 
   Size:        222     122      58     573     316

  Fragments arranged by size: 

                1460     807     573     316     265     255     222     158
                 153     135     122      75      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3830    4599 
   Size:       3830     769
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3043    3588    4599 
   Size:       3043     545    1011

  Fragments arranged by size: 

                3043    1011     545
 
EcoRI G'AATT_C

Cuts at:      0    1362    4599 
   Size:       1362    3237
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3481    4599 
   Size:       3481    1118
 
HaeII r_GCGC'y

Cuts at:      0    2921    4599 
   Size:       2921    1678
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1009    1023    1041    1055    1603    1635    1931    1963 
   Size:       1009      14      18      14     548      32     296      32

Cuts at:   1963    2240    2272    2493    2525    3766    3798    4016    4048 
   Size:        277      32     221      32    1241      32     218      32

Cuts at:   4048    4561    4593    4599 
   Size:        513      32       6

  Fragments arranged by size: 

                1241    1009     548     513     296     277     221     218
                  32      32      32      32      32      32      32      18
                  14      14       6
 
HincII GTy'rAC

Cuts at:      0    1728    2982    4599 
   Size:       1728    1254    1617

  Fragments arranged by size: 

                1728    1617    1254
 
HindIII A'AGCT_T

Cuts at:      0    1342    1372    4599 
   Size:       1342      30    3227

  Fragments arranged by size: 

                3227    1342      30
 
HpaI GTT'AAC

Cuts at:      0    2982    4599 
   Size:       2982    1617
 
KasI G'GCGC_C

Cuts at:      0    2917    4599 
   Size:       2917    1682
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      99    3319    4163    4599 
   Size:         99    3220     844     436

  Fragments arranged by size: 

                3220     844     436      99
 
MscI TGG'CCA

Cuts at:      0    1283    4599 
   Size:       1283    3316
 
MslI CAynn'nnrTG

Cuts at:      0     674    3872    4018    4599 
   Size:        674    3198     146     581

  Fragments arranged by size: 

                3198     674     581     146
 
MspA1I CmG'CkG

Cuts at:      0    3328    4000    4261    4599 
   Size:       3328     672     261     338

  Fragments arranged by size: 

                3328     672     338     261
 
NaeI GCC'GGC

Cuts at:      0    3098    3579    4599 
   Size:       3098     481    1020

  Fragments arranged by size: 

                3098    1020     481
 
NarI GG'CG_CC

Cuts at:      0    2918    4599 
   Size:       2918    1681
 
NcoI C'CATG_G

Cuts at:      0     209    3406    4599 
   Size:        209    3197    1193

  Fragments arranged by size: 

                3197    1193     209
 
NgoMIV G'CCGG_C

Cuts at:      0    3096    3577    4599 
   Size:       3096     481    1022

  Fragments arranged by size: 

                3096    1022     481
 
NspI r_CATG'y

Cuts at:      0     378    1806    4439    4599 
   Size:        378    1428    2633     160

  Fragments arranged by size: 

                2633    1428     378     160
 
PciI A'CATG_T

Cuts at:      0    4435    4599 
   Size:       4435     164
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2245    3407    4599 
   Size:       2245    1162    1192

  Fragments arranged by size: 

                2245    1192    1162
 
PfoI T'CCnGG_A

Cuts at:      0    2550    4599 
   Size:       2550    2049
 
PmlI CAC'GTG

Cuts at:      0     151    4599 
   Size:        151    4448
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3433    3465    4599 
   Size:       3433      32    1134

  Fragments arranged by size: 

                3433    1134      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    3043    4599 
   Size:       3043    1556
 
PspXI vC'TCGA_Gb

Cuts at:      0    4525    4599 
   Size:       4525      74
 
PstI C_TGCA'G

Cuts at:      0     498    1602    4599 
   Size:        498    1104    2997

  Fragments arranged by size: 

                2997    1104     498
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1217    1766    4599 
   Size:       1217     549    2833

  Fragments arranged by size: 

                2833    1217     549
 
ScaI AGT'ACT

Cuts at:      0    1454    4599 
   Size:       1454    3145
 
SexAI A'CCwGG_T

Cuts at:      0    4389    4599 
   Size:       4389     210
 
SfcI C'TryA_G

Cuts at:      0     494    1598    3053    4599 
   Size:        494    1104    1455    1546

  Fragments arranged by size: 

                1546    1455    1104     494
 
SfoI GGC'GCC

Cuts at:      0    2919    4599 
   Size:       2919    1680
 
SmlI C'TyrA_G

Cuts at:      0     784    3455    4525    4599 
   Size:        784    2671    1070      74

  Fragments arranged by size: 

                2671    1070     784      74
 
SpeI A'CTAG_T

Cuts at:      0     130     790    4599 
   Size:        130     660    3809

  Fragments arranged by size: 

                3809     660     130
 
SphI G_CATG'C

Cuts at:      0     378    1806    4599 
   Size:        378    1428    2793

  Fragments arranged by size: 

                2793    1428     378
 
SspI AAT'ATT

Cuts at:      0    2128    4599 
   Size:       2128    2471
 
StyI C'CwwG_G

Cuts at:      0     209    1278    3406    3584    4599 
   Size:        209    1069    2128     178    1015

  Fragments arranged by size: 

                2128    1069    1015     209     178
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     477    3007    3952    4599 
   Size:        477    2530     945     647

  Fragments arranged by size: 

                2530     945     647     477
 
TatI w'GTAC_w

Cuts at:      0    1434    1452    4599 
   Size:       1434      18    3147

  Fragments arranged by size: 

                3147    1434      18
 
XhoI C'TCGA_G

Cuts at:      0    4525    4599 
   Size:       4525      74
 
XmnI GAAnn'nnTTC

Cuts at:      0     474    1752    2627    3464    4599 
   Size:        474    1278     875     837    1135

  Fragments arranged by size: 

                1278    1135     875     837     474
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AhdI     AlfI    AlwNI    ApaLI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrDI    BsrFI   BstAPI    BstXI    BstYI  BstZ17I     BtgI     BtsI
     ClaI     DraI   DraIII     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PspXI     PstI     SapI     ScaI    SexAI     SfcI     SfoI     SmlI
     SpeI     SphI     SspI     StyI    TaqII     TatI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI     AloI
     ApaI     AscI     AseI    AsiSI    AvrII    BciVI    BfrBI     BglI
    BmgBI     BmtI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI
    BsrGI   BssHII    BssSI    BstBI   BstEII   Bsu36I    BtgZI    CspCI
     DrdI     EagI  EcoICRI    EcoRV     FseI     FspI    FspAI     KpnI
     MfeI     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PmeI    PshAI     PsiI   PspOMI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SrfI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XmaI     ZraI
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