Restriction map for pF1KA0581
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AgeI        AhdI        Aor51HI     ApaI        AscI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BlnI        BsaBI       BsaI        
BseAI       BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BsrBI       BsrDI       BsrFI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRV       FauI        
FseI        FspI        HpaI        KpnI        KspI        
MamI        MluI        MroI        MvnI        NaeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PflFI       PinAI       PmeI        Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SbfI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        Tth111I     XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI         2371  GTCTAC               GT^[AC][GT]AC
AcyI          287  GGCGCC               G[GA]^CG[CT]C
Alw44I         22  GTGCAC               G^TGCAC
AocI          139  CCTCAGG              CC^T[ACGT]AGG
ApaLI          22  GTGCAC               G^TGCAC
AseI          979  ATTAAT               AT^TAAT
AsnI          979  ATTAAT               AT^TAAT
AvaI         3475  CTCGAG               C^[CT]CG[GA]G
BbeI          287  GGCGCC               GGCGC^C
BbrPI        1805  CACGTG               CAC^GTG
BbvCI        1831  CCTCAGC              CCTCAGC(-5/-2)
BciVI        2118  GGATAC               GTATCC(6/5)
BfaI         3337  CTAG                 C^TAG
BglI         1264  GCCAAGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2685  AGATCT               A^GATCT
BsaHI         287  GGCGCC               G[GA]^CG[CT]C
BsaWI        3296  TCCGGT               [AT]^CCGG[AT]
BsmI         1845  GAATGC               GAATGC(1/-1)
BsoBI        3475  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      632  TGTACA               T^GTACA
BspHI        1961  TCATGA               T^CATGA
BsrGI         632  TGTACA               T^GTACA
BstXI        1014  CCAACTGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        139  CCTCAGG              CC^T[ACGT]AGG
DraII         245  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI         1131  CCATGG               C^C[GA][CT]GG
Eco81I        139  CCTCAGG              CC^T[ACGT]AGG
EcoNI          63  CCTCAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      245  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI        3487  GAATTC               G^AATTC
EcoT22I       446  ATGCAT               ATGCA^T
HaeII         287  GGCGCC               [GA]GCGC^[CT]
HgaI         2352  GACGC                GACGC(5/10)
Hin1I         287  GGCGCC               G[GA]^CG[CT]C
HincII       3123  GTTGAC               GT[CT]^[GA]AC
HindII       3123  GTTGAC               GT[CT]^[GA]AC
HindIII      2423  AAGCTT               A^AGCTT
KasI          287  GGCGCC               G^GCGCC
MaeI         3337  CTAG                 C^TAG
NarI          287  GGCGCC               GG^CGCC
NcoI         1131  CCATGG               C^CATGG
NdeI         2405  CATATG               CA^TATG
NsiI          446  ATGCAT               ATGCA^T
PaeR7I       3475  CTCGAG               C^TCGAG
PmaCI        1805  CACGTG               CAC^GTG
PmlI         1805  CACGTG               CAC^GTG
PpuMI         245  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI         979  ATTAAT               AT^TAAT
RcaI         1961  TCATGA               T^CATGA
SapI         1836  GCTCTTC              GCTCTTC(1/4)
ScaI         1277  AGTACT               AGT^ACT
SexAI        2795  ACCTGGT              A^CC[AT]GGT
SfoI          287  GGCGCC               GGC^GCC
SspBI         632  TGTACA               T^GTACA
StuI         1969  AGGCCT               AGG^CCT
XhoI         3475  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          633  GTAC                 GT^AC
             1278  GTAC                
AflII         475  CTTAAG               C^TTAAG
              751  CTTAAG              
AspHI          22  GTGCAC               G[AT]GC[AT]^C
             1054  GTGCTC              
AvaII         246  GGACC                G^G[AT]CC
             1887  GGACC               
BcgI           74  GCACCAAATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2292  GCAAGCCATTCG        
BcnI            5  CCGGG                CC^[GC]GG
               16  CCCGG               
BfrI          475  CTTAAG               C^TTAAG
              751  CTTAAG              
BlpI         1779  GCTTAGC              GC^T[ACGT]AGC
             2470  GCTAAGC             
Bpu1102I     1779  GCTTAGC              GC^T[ACGT]AGC
             2470  GCTAAGC             
BsgI         3319  GTGCAG               GTGCAG(16/14)
             3493  GTGCAG              
BsiHKAI        22  GTGCAC               G[AT]GC[AT]^C
             1054  GTGCTC              
BspLU11I      434  ACATGT               A^CATGT
              950  ACATGT              
BssSI         331  CTCGTG               CACGAG(-5/-1)
              355  CTCGTG              
BstAPI         24  GCACGCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3039  GCAGCAGCTGC         
CelII        1779  GCTTAGC              GC^T[ACGT]AGC
             2470  GCTAAGC             
MfeI         1181  CAATTG               C^AATTG
             2476  CAATTG              
MslI          309  CACAGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2376  CATAGAAGTG          
MspA1I       1007  CAGCTG               C[AC]G^C[GT]G
             3043  CAGCTG              
MunI         1181  CAATTG               C^AATTG
             2476  CAATTG              
NciI            5  CCGGG                CC^[GC]GG
               16  CCCGG               
PshAI        3126  GACGGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3259  GACAAAAGTC          
PstI         1106  CTGCAG               CTGCA^G
             3424  CTGCAG              
PvuII        1007  CAGCTG               CAG^CTG
             3043  CAGCTG              
RsaI          633  GTAC                 GT^AC
             1278  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AciI        AcsI        AflIII      AluI        AlwI        
AlwNI       ApoI        BalI        BanI        BanII       
BbsI        BbvI        BclI        BmyI        BpmI        
BpuAI       BsaAI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EaeI        EarI        EcoRII      EcoT14I     FbaI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       
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