Restriction map for pF1KA0581
 (Linear) (Six-base) MAPSORT of: KIAA0581.seq  Check: 1500  from: 1  to: 3648



KIAA0581.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 16:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2679    3417    3648 
   Size:       2679     738     231

  Fragments arranged by size: 

                2679     738     231
 
AccI GT'mk_AC

Cuts at:      0    2372    3648 
   Size:       2372    1276
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2018    3648 
   Size:       2018    1630
 
AflII C'TTAA_G

Cuts at:      0     475     751    3648 
   Size:        475     276    2897

  Fragments arranged by size: 

                2897     475     276
 
AflIII A'CryG_T

Cuts at:      0     434     950    1804    1806    3648 
   Size:        434     516     854       2    1842

  Fragments arranged by size: 

                1842     854     516     434       2
 
AleI CACnn'nnGTG

Cuts at:      0     313    3648 
   Size:        313    3335
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2498    3648 
   Size:       2498    1150
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2349    2659    3045    3405    3568    3648 
   Size:       2349     310     386     360     163      80

  Fragments arranged by size: 

                2349     386     360     310     163      80
 
ApaLI G'TGCA_C

Cuts at:      0      22    3648 
   Size:         22    3626
 
ApoI r'AATT_y

Cuts at:      0      79     691    1382    1667    1753    2263    2861    3457 
   Size:         79     612     691     285      86     510     598     596

Cuts at:   3457    3487    3631    3648 
   Size:         30     144      17

  Fragments arranged by size: 

                 691     612     598     596     510     285     144      86
                  79      30      17
 
AseI AT'TA_AT

Cuts at:      0     980    3648 
   Size:        980    2668
 
AvaI C'yCGr_G

Cuts at:      0    3475    3648 
   Size:       3475     173
 
BanI G'GyrC_C

Cuts at:      0      73     287    2667    3648 
   Size:         73     214    2380     981

  Fragments arranged by size: 

                2380     981     214      73
 
BanII G_rGCy'C

Cuts at:      0      12    1319    1496    3648 
   Size:         12    1307     177    2152

  Fragments arranged by size: 

                2152    1307     177      12
 
BbeI G_GCGC'C

Cuts at:      0     291    3648 
   Size:        291    3357
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     571     953    1694    3289    3530    3648 
   Size:        571     382     741    1595     241     118

  Fragments arranged by size: 

                1595     741     571     382     241     118
 
BbvCI CC'TCA_GC

Cuts at:      0    1832    3648 
   Size:       1832    1816
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      63      97    2281    2315    3648 
   Size:         63      34    2184      34    1333

  Fragments arranged by size: 

                2184    1333      63      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2112    3648 
   Size:       2112    1536
 
BclI T'GATC_A

Cuts at:      0     305     726    2949    3648 
   Size:        305     421    2223     699

  Fragments arranged by size: 

                2223     699     421     305
 
BfrBI ATG'CAT

Cuts at:      0     448    3648 
   Size:        448    3200
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1270    3648 
   Size:       1270    2378
 
BglII A'GATC_T

Cuts at:      0    2685    3648 
   Size:       2685     963
 
BlpI GC'TnA_GC

Cuts at:      0    1780    2471    3648 
   Size:       1780     691    1177

  Fragments arranged by size: 

                1780    1177     691
 
Bme1580I G_kGCm'C

Cuts at:      0      26      76    3648 
   Size:         26      50    3572

  Fragments arranged by size: 

                3572      50      26
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     661    2588    3648 
   Size:        661    1927    1060

  Fragments arranged by size: 

                1927    1060     661
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     476     608    1364    1464    1730    2552    2558 
   Size:        313     163     132     756     100     266     822       6

Cuts at:   2558    2636    3457    3579    3648 
   Size:         78     821     122      69

  Fragments arranged by size: 

                 822     821     756     313     266     163     132     122
                 100      78      69       6
 
Bpu10I CC'TnA_GC

Cuts at:      0    1832    3648 
   Size:       1832    1816
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22      49     593    2898    3648 
   Size:         22      27     544    2305     750

  Fragments arranged by size: 

                2305     750     544      27      22
 
BsaAI yAC'GTr

Cuts at:      0     259    1807    2088    3648 
   Size:        259    1548     281    1560

  Fragments arranged by size: 

                1560    1548     281     259
 
BsaHI Gr'CG_yC

Cuts at:      0     288    3648 
   Size:        288    3360
 
BsaWI w'CCGG_w

Cuts at:      0    3296    3648 
   Size:       3296     352
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1459    1489    1728    1758    3648 
   Size:       1459      30     239      30    1890

  Fragments arranged by size: 

                1890    1459     239      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1020    1467    1980    3292    3616    3626    3632    3648 
   Size:       1020     447     513    1312     324      10       6      16

  Fragments arranged by size: 

                1312    1020     513     447     324      16      10       6
            
 
BseYI C'CCAG_C

Cuts at:      0    2938    3606    3648 
   Size:       2938     668      42

  Fragments arranged by size: 

                2938     668      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3340    3514    3648 
   Size:       3340     174     134

  Fragments arranged by size: 

                3340     174     134
 
BsiHKAI G_wGCw'C

Cuts at:      0      26    1058    3648 
   Size:         26    1032    2590

  Fragments arranged by size: 

                2590    1032      26
 
BsmI GAATG_Cn'

Cuts at:      0    1851    3648 
   Size:       1851    1797
 
Bsp1286I G_dGCh'C

Cuts at:      0      12      26      76    1058    1319    1496    3648 
   Size:         12      14      50     982     261     177    2152

  Fragments arranged by size: 

                2152     982     261     177      50      14      12
 
BspHI T'CATG_A

Cuts at:      0    1961    3648 
   Size:       1961    1687
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     428    1840    2679    3417    3648 
   Size:        428    1412     839     738     231

  Fragments arranged by size: 

                1412     839     738     428     231
 
BsrGI T'GTAC_A

Cuts at:      0     632    3648 
   Size:        632    3016
 
BssSI C'ACGA_G

Cuts at:      0     331     355    3648 
   Size:        331      24    3293

  Fragments arranged by size: 

                3293     331      24
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      30    3045    3648 
   Size:         30    3015     603

  Fragments arranged by size: 

                3015     603      30
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1021    3648 
   Size:       1021    2627
 
BstYI r'GATC_y

Cuts at:      0     192     762    2685    3401    3648 
   Size:        192     570    1923     716     247

  Fragments arranged by size: 

                1923     716     570     247     192
 
Bsu36I CC'TnA_GG

Cuts at:      0     140    3648 
   Size:        140    3508
 
BtgI C'CryG_G

Cuts at:      0    1131    3648 
   Size:       1131    2517
 
EaeI y'GGCC_r

Cuts at:      0       2     377    1011    1604    2271    2302    3648 
   Size:          2     375     634     593     667      31    1346

  Fragments arranged by size: 

                1346     667     634     593     375      31       2
 
EarI CTCTTCn'nnn_

Cuts at:      0    1106    1358    1843    1927    3134    3269    3648 
   Size:       1106     252     485      84    1207     135     379

  Fragments arranged by size: 

                1207    1106     485     379     252     135      84
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1288    2895    3648 
   Size:       1288    1607     753

  Fragments arranged by size: 

                1607    1288     753
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     476     608    1364    1464    1730    2018    2552 
   Size:        313     163     132     756     100     266     288     534

Cuts at:   2552    2558    2636    3457    3579    3648 
   Size:          6      78     821     122      69

  Fragments arranged by size: 

                 821     756     534     313     288     266     163     132
                 122     100      78      69       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      67    3648 
   Size:         67    3581
 
EcoO109I rG'GnC_Cy

Cuts at:      0     246    3648 
   Size:        246    3402
 
EcoRI G'AATT_C

Cuts at:      0    3487    3648 
   Size:       3487     161
 
HaeII r_GCGC'y

Cuts at:      0     291    3648 
   Size:        291    3357
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      85     117     123     155    1483    1515    3458    3490 
   Size:         85      32       6      32    1328      32    1943      32

Cuts at:   3490    3602    3634    3648 
   Size:        112      32      14

  Fragments arranged by size: 

                1943    1328     112      85      32      32      32      32
                  32      14       6
 
HincII GTy'rAC

Cuts at:      0    3125    3648 
   Size:       3125     523
 
HindIII A'AGCT_T

Cuts at:      0    2423    3648 
   Size:       2423    1225
 
KasI G'GCGC_C

Cuts at:      0     287    3648 
   Size:        287    3361
 
MfeI C'AATT_G

Cuts at:      0    1181    2476    3648 
   Size:       1181    1295    1172

  Fragments arranged by size: 

                1295    1181    1172
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      80    1484    1844    3648 
   Size:         80    1404     360    1804

  Fragments arranged by size: 

                1804    1404     360      80
 
MscI TGG'CCA

Cuts at:      0     379    1013    1606    2273    3648 
   Size:        379     634     593     667    1375

  Fragments arranged by size: 

                1375     667     634     593     379
 
MslI CAynn'nnrTG

Cuts at:      0     313    2380    3648 
   Size:        313    2067    1268

  Fragments arranged by size: 

                2067    1268     313
 
MspA1I CmG'CkG

Cuts at:      0    1009    3045    3648 
   Size:       1009    2036     603

  Fragments arranged by size: 

                2036    1009     603
 
NarI GG'CG_CC

Cuts at:      0     288    3648 
   Size:        288    3360
 
NcoI C'CATG_G

Cuts at:      0    1131    3648 
   Size:       1131    2517
 
NdeI CA'TA_TG

Cuts at:      0    2406    3648 
   Size:       2406    1242
 
NsiI A_TGCA'T

Cuts at:      0     450    3648 
   Size:        450    3198
 
NspI r_CATG'y

Cuts at:      0     438     954    1488    3648 
   Size:        438     516     534    2160

  Fragments arranged by size: 

                2160     534     516     438
 
PciI A'CATG_T

Cuts at:      0     434     950    3648 
   Size:        434     516    2698

  Fragments arranged by size: 

                2698     516     434
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     386    1884    3315    3648 
   Size:        386    1498    1431     333

  Fragments arranged by size: 

                1498    1431     386     333
 
PfoI T'CCnGG_A

Cuts at:      0    2529    3308    3404    3648 
   Size:       2529     779      96     244

  Fragments arranged by size: 

                2529     779     244      96
 
PmlI CAC'GTG

Cuts at:      0    1807    3648 
   Size:       1807    1841
 
PpuMI rG'GwC_Cy

Cuts at:      0     246    3648 
   Size:        246    3402
 
PshAI GACnn'nnGTC

Cuts at:      0    3130    3263    3648 
   Size:       3130     133     385

  Fragments arranged by size: 

                3130     385     133
 
PstI C_TGCA'G

Cuts at:      0    1110    3428    3648 
   Size:       1110    2318     220

  Fragments arranged by size: 

                2318    1110     220
 
PvuII CAG'CTG

Cuts at:      0    1009    3045    3648 
   Size:       1009    2036     603

  Fragments arranged by size: 

                2036    1009     603
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1843    3648 
   Size:       1843    1805
 
ScaI AGT'ACT

Cuts at:      0    1279    3648 
   Size:       1279    2369
 
SexAI A'CCwGG_T

Cuts at:      0    2795    3648 
   Size:       2795     853
 
SfcI C'TryA_G

Cuts at:      0    1106    1467    1941    3424    3648 
   Size:       1106     361     474    1483     224

  Fragments arranged by size: 

                1483    1106     474     361     224
 
SfoI GGC'GCC

Cuts at:      0     289    3648 
   Size:        289    3359
 
SmlI C'TyrA_G

Cuts at:      0      37      64     475     608     751    2877    3475    3648 
   Size:         37      27     411     133     143    2126     598     173

  Fragments arranged by size: 

                2126     598     411     173     143     133      37      27
            
 
StuI AGG'CCT

Cuts at:      0    1971    3648 
   Size:       1971    1677
 
StyI C'CwwG_G

Cuts at:      0     242     380    1131    2154    3648 
   Size:        242     138     751    1023    1494

  Fragments arranged by size: 

                1494    1023     751     242     138
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1144    2628    3648 
   Size:       1144    1484    1020

  Fragments arranged by size: 

                1484    1144    1020
 
TatI w'GTAC_w

Cuts at:      0     632    1277    3648 
   Size:        632     645    2371

  Fragments arranged by size: 

                2371     645     632
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1728    1760    1793    1825    1859    1891    2150    2182 
   Size:       1728      32      33      32      34      32     259      32

Cuts at:   2182    3648 
   Size:       1466

  Fragments arranged by size: 

                1728    1466     259      34      33      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1746    3426    3603    3648 
   Size:       1746    1680     177      45

  Fragments arranged by size: 

                1746    1680     177      45
 
XhoI C'TCGA_G

Cuts at:      0    3475    3648 
   Size:       3475     173
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AleI     AlfI    AlwNI
    ApaLI     ApoI     AseI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrGI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII
    Hin4I   HincII  HindIII     KasI     MfeI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NsiI     NspI     PciI    PflMI
     PfoI     PmlI    PpuMI    PshAI     PstI    PvuII     SapI     ScaI
    SexAI     SfcI     SfoI     SmlI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AloI     ApaI
     AscI    AsiSI    AvrII     BaeI    BamHI    BmgBI     BmrI     BmtI
     BsaI    BsaBI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI
    BsrFI   BssHII    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoRV     FalI
     FseI     FspI    FspAI     HpaI     KpnI     MluI     NaeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PmeI     PpiI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XmaI     XmnI
     ZraI
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