Restriction map for pF1KA0461
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      ApaI        
ApaLI       AscI        AseI        AsnI        AspEI       
AspHI       AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BfrI        BlnI        
BsaBI       BsaHI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BspDI       BspEI       BsrBI       
BssHII      Bst1107I    BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoO109I    
EcoO65I     EcoRI       EcoRV       FseI        FspI        
HgaI        Hin1I       HpaI        KasI        KspI        
MamI        MluI        MroI        NarI        NotI        
NruI        PacI        PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SbfI        SfiI        SfoI        SgrAI       SmaI        
Sse8387I    SwaI        Van91I      XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

Acc65I        415  GGTACC               G^GTACC
AccII        2956  CGCG                 CG^CG
AflIII       1019  ACATGT               A^C[GA][CT]GT
Aor51HI      3800  AGCGCT               AGC^GCT
Asp718I       415  GGTACC               G^GTACC
BsaWI        2441  ACCGGA               [AT]^CCGG[AT]
BspHI        1367  TCATGA               T^CATGA
BspLU11I     1019  ACATGT               A^CATGT
BssSI        2423  CTCGTG               CACGAG(-5/-1)
BstBI         551  TTCGAA               TT^CGAA
BstUI        2956  CGCG                 CG^CG
DraI         2257  TTTAAA               TTT^AAA
Eco47III     3800  AGCGCT               AGC^GCT
EcoNI        3117  CCTACCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII       3465  GTCAAC               GT[CT]^[GA]AC
HindII       3465  GTCAAC               GT[CT]^[GA]AC
KpnI          415  GGTACC               GGTAC^C
MfeI         3974  CAATTG               C^AATTG
MunI         3974  CAATTG               C^AATTG
MvnI         2956  CGCG                 CG^CG
NcoI           49  CCATGG               C^CATGG
NspV          551  TTCGAA               TT^CGAA
PshAI         899  GACCTGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1367  TCATGA               T^CATGA
ScaI         2781  AGTACT               AGT^ACT
SexAI         759  ACCTGGT              A^CC[AT]GGT
SfuI          551  TTCGAA               TT^CGAA
SnaBI        2149  TACGTA               TAC^GTA
SphI         3512  GCATGC               GCATG^C
XbaI         2892  TCTAGA               T^CTAGA
XcmI          818  CCATCCCTTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AlwNI        1127  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3704  CAGATACTG           
AocI         1303  CCTAAGG              CC^T[ACGT]AGG
             3121  CCTAAGG             
AspI          585  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4020  GACCGAGTC           
BanII        2235  GAGCCC               G[GA]GC[CT]^C
             2685  GAGCCC              
BclI         2210  TGATCA               T^GATCA
             2670  TGATCA              
BlpI         2524  GCTGAGC              GC^T[ACGT]AGC
             3249  GCTGAGC             
Bpu1102I     2524  GCTGAGC              GC^T[ACGT]AGC
             3249  GCTGAGC             
BsaAI        1387  CACGTA               [CT]AC^GT[GA]
             2149  TACGTA              
BsaI         2983  GAGACC               GGTCTC(1/5)
             4018  GAGACC              
BsgI         2289  CTGCAC               GTGCAG(16/14)
             2106  GTGCAG              
Bsp1407I      327  TGTACA               T^GTACA
             3162  TGTACA              
BspMI         720  ACCTGC               ACCTGC(4/8)
             2179  ACCTGC              
BsrFI        1796  GCCGGC               [GA]^CCGG[CT]
             2087  GCCGGC              
BsrGI         327  TGTACA               T^GTACA
             3162  TGTACA              
Bsu36I       1303  CCTAAGG              CC^T[ACGT]AGG
             3121  CCTAAGG             
CelII        2524  GCTGAGC              GC^T[ACGT]AGC
             3249  GCTGAGC             
Cfr10I       1796  GCCGGC               [GA]^CCGG[CT]
             2087  GCCGGC              
Eco81I       1303  CCTAAGG              CC^T[ACGT]AGG
             3121  CCTAAGG             
EcoT22I      1736  ATGCAT               ATGCA^T
             3639  ATGCAT              
FauI         2610  CCCGC                CCCGC(4/6)
             2509  GCGGG               
FbaI         2210  TGATCA               T^GATCA
             2670  TGATCA              
HaeII        1969  AGCGCC               [GA]GCGC^[CT]
             3800  AGCGCT              
MslI         1896  CACCAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3513  CATGCTTGTG          
NaeI         1796  GCCGGC               GCC^GGC
             2087  GCCGGC              
NdeI         1375  CATATG               CA^TATG
             1573  CATATG              
NgoMIV       1796  GCCGGC               G^CCGGC
             2087  GCCGGC              
NheI          696  GCTAGC               G^CTAGC
             2709  GCTAGC              
NsiI         1736  ATGCAT               ATGCA^T
             3639  ATGCAT              
PaeR7I       2189  CTCGAG               C^TCGAG
             3470  CTCGAG              
PflFI         585  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4020  GACCGAGTC           
SapI         2232  GAAGAGC              GCTCTTC(1/4)
             2533  GAAGAGC             
SpeI          256  ACTAGT               A^CTAGT
              399  ACTAGT              
SspBI         327  TGTACA               T^GTACA
             3162  TGTACA              
SspI          282  AATATT               AAT^ATT
             1357  AATATT              
StuI          220  AGGCCT               AGG^CCT
             2873  AGGCCT              
Tth111I       585  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4020  GACCGAGTC           
XhoI         2189  CTCGAG               C^TCGAG
             3470  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AvaI        AvaII       BalI        
BanI        BbsI        BbvI        BciVI       BcnI        
BfaI        BglI        BglII       BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BsrDI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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