Restriction map for pF1KA0461
 (Linear) (Six-base) MAPSORT of: KIAA0461.seq  Check: 6906  from: 1  to: 4071



KIAA0461.orf

 With 182 enzymes: SgfI * 

                        September 25, 2009 11:41  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     729    2188    4071 
   Size:        729    1459    1883

  Fragments arranged by size: 

                1883    1459     729
 
Acc65I G'GTAC_C

Cuts at:      0     415    4071 
   Size:        415    3656
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1022    1459    1735    2552    3718    3931    3986    4071 
   Size:       1022     437     276     817    1166     213      55      85

  Fragments arranged by size: 

                1166    1022     817     437     276     213      85      55
            
 
AfeI AGC'GCT

Cuts at:      0    3802    4071 
   Size:       3802     269
 
AflIII A'CryG_T

Cuts at:      0    1019    4071 
   Size:       1019    3052
 
AleI CACnn'nnGTG

Cuts at:      0    1900    4071 
   Size:       1900    2171
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2392    3529    4071 
   Size:       2392    1137     542

  Fragments arranged by size: 

                2392    1137     542
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1073    1105    2974    3006    4071 
   Size:       1073      32    1869      32    1065

  Fragments arranged by size: 

                1869    1073    1065      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1132    3709    4071 
   Size:       1132    2577     362

  Fragments arranged by size: 

                2577    1132     362
 
ApoI r'AATT_y

Cuts at:      0    1296    2262    3620    4071 
   Size:       1296     966    1358     451

  Fragments arranged by size: 

                1358    1296     966     451
 
AvaI C'yCGr_G

Cuts at:      0    1919    2189    3470    3692    4071 
   Size:       1919     270    1281     222     379

  Fragments arranged by size: 

                1919    1281     379     270     222
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1172    1205    3230    3263    4071 
   Size:       1172      33    2025      33     808

  Fragments arranged by size: 

                2025    1172     808      33      33
 
BanI G'GyrC_C

Cuts at:      0     415    1760    1894    3074    4071 
   Size:        415    1345     134    1180     997

  Fragments arranged by size: 

                1345    1180     997     415     134
 
BanII G_rGCy'C

Cuts at:      0    2239    2689    4071 
   Size:       2239     450    1382

  Fragments arranged by size: 

                2239    1382     450
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     899    1713    2013    3929    3982    4071 
   Size:        899     814     300    1916      53      89

  Fragments arranged by size: 

                1916     899     814     300      89      53
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1575    1677    1956    3231    4071 
   Size:       1575     102     279    1275     840

  Fragments arranged by size: 

                1575    1275     840     279     102
 
BclI T'GATC_A

Cuts at:      0    2210    2670    4071 
   Size:       2210     460    1401

  Fragments arranged by size: 

                2210    1401     460
 
BfrBI ATG'CAT

Cuts at:      0    1738    3641    4071 
   Size:       1738    1903     430

  Fragments arranged by size: 

                1903    1738     430
 
BglI GCCn_nnn'nGGC

Cuts at:      0     501    3331    3839    4071 
   Size:        501    2830     508     232

  Fragments arranged by size: 

                2830     508     501     232
 
BglII A'GATC_T

Cuts at:      0    1529    3038    3221    4056    4071 
   Size:       1529    1509     183     835      15

  Fragments arranged by size: 

                1529    1509     835     183      15
 
BlpI GC'TnA_GC

Cuts at:      0    2525    3250    4071 
   Size:       2525     725     821

  Fragments arranged by size: 

                2525     821     725
 
Bme1580I G_kGCm'C

Cuts at:      0     196    3295    4071 
   Size:        196    3099     776

  Fragments arranged by size: 

                3099     776     196
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     414     567     649     928    1079    2683    4071 
   Size:        414     153      82     279     151    1604    1388

  Fragments arranged by size: 

                1604    1388     414     279     153     151      82
 
BmtI G_CTAG'C

Cuts at:      0     700    2713    4071 
   Size:        700    2013    1358

  Fragments arranged by size: 

                2013    1358     700
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     647     931    1552    1616    1897    2122    4071 
   Size:        647     284     621      64     281     225    1949

  Fragments arranged by size: 

                1949     647     621     284     281     225      64
 
Bpu10I CC'TnA_GC

Cuts at:      0    2550    2705    4071 
   Size:       2550     155    1366

  Fragments arranged by size: 

                2550    1366     155
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2391    4071 
   Size:       2391    1680
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2977    4012    4071 
   Size:       2977    1035      59

  Fragments arranged by size: 

                2977    1035      59
 
BsaAI yAC'GTr

Cuts at:      0    1389    2151    4071 
   Size:       1389     762    1920

  Fragments arranged by size: 

                1920    1389     762
 
BsaWI w'CCGG_w

Cuts at:      0    2441    4071 
   Size:       2441    1630
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25      55    1550    1580    2743    2773    2974    3004 
   Size:         25      30    1495      30    1163      30     201      30

Cuts at:   3004    3448    3478    4071 
   Size:        444      30     593

  Fragments arranged by size: 

                1495    1163     593     444     201      30      30      30
                  30      30      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46      49      52    1626    1989    2456    2995    3895 
   Size:         46       3       3    1574     363     467     539     900

Cuts at:   3895    3916    4071 
   Size:         21     155

  Fragments arranged by size: 

                1574     900     539     467     363     155      46      21
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0     578     598    1286    1966    2520    2556    2837    2951 
   Size:        578      20     688     680     554      36     281     114

Cuts at:   2951    3600    3753    4071 
   Size:        649     153     318

  Fragments arranged by size: 

                 688     680     649     578     554     318     281     153
                 114      36      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2127    2274    4071 
   Size:       2127     147    1797

  Fragments arranged by size: 

                2127    1797     147
 
BsmI GAATG_Cn'

Cuts at:      0     246    1738    3144    3279    4071 
   Size:        246    1492    1406     135     792

  Fragments arranged by size: 

                1492    1406     792     246     135
 
Bsp1286I G_dGCh'C

Cuts at:      0     196    2239    2689    3295    4071 
   Size:        196    2043     450     606     776

  Fragments arranged by size: 

                2043     776     606     450     196
 
BspHI T'CATG_A

Cuts at:      0    1367    4071 
   Size:       1367    2704
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     729    2188    4071 
   Size:        729    1459    1883

  Fragments arranged by size: 

                1883    1459     729
 
BsrDI GCAATG_nn'

Cuts at:      0     344     731     789    1740    4071 
   Size:        344     387      58     951    2331

  Fragments arranged by size: 

                2331     951     387     344      58
 
BsrFI r'CCGG_y

Cuts at:      0    1796    2087    4071 
   Size:       1796     291    1984

  Fragments arranged by size: 

                1984    1796     291
 
BsrGI T'GTAC_A

Cuts at:      0     327    3162    4071 
   Size:        327    2835     909

  Fragments arranged by size: 

                2835     909     327
 
BssSI C'ACGA_G

Cuts at:      0    2423    4071 
   Size:       2423    1648
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1793    3139    3709    4071 
   Size:       1793    1346     570     362

  Fragments arranged by size: 

                1793    1346     570     362
 
BstBI TT'CG_AA

Cuts at:      0     552    4071 
   Size:        552    3519
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     148     841     955    1875    2247    2340    4071 
   Size:        148     693     114     920     372      93    1731

  Fragments arranged by size: 

                1731     920     693     372     148     114      93
 
BstYI r'GATC_y

Cuts at:      0    1529    2117    2362    3038    3221    4056    4071 
   Size:       1529     588     245     676     183     835      15

  Fragments arranged by size: 

                1529     835     676     588     245     183      15
 
Bsu36I CC'TnA_GG

Cuts at:      0    1304    3122    4071 
   Size:       1304    1818     949

  Fragments arranged by size: 

                1818    1304     949
 
BtgI C'CryG_G

Cuts at:      0      49    2227    2380    4071 
   Size:         49    2178     153    1691

  Fragments arranged by size: 

                2178    1691     153      49
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1229    2336    3517    4071 
   Size:       1229    1107    1181     554

  Fragments arranged by size: 

                1229    1181    1107     554
 
BtsI GCAGTG_nn'

Cuts at:      0     617     731    1782    2154    3261    3599    4071 
   Size:        617     114    1051     372    1107     338     472

  Fragments arranged by size: 

                1107    1051     617     472     372     338     114
 
DraI TTT'AAA

Cuts at:      0    2259    4071 
   Size:       2259    1812
 
EaeI y'GGCC_r

Cuts at:      0     536     791    2330    2415    4071 
   Size:        536     255    1539      85    1656

  Fragments arranged by size: 

                1656    1539     536     255      85
 
EarI CTCTTCn'nnn_

Cuts at:      0     930    1155    1762    2050    2227    2528    2918    3154 
   Size:        930     225     607     288     177     301     390     236

Cuts at:   3154    3545    3962    4071 
   Size:        391     417     109

  Fragments arranged by size: 

                 930     607     417     391     390     301     288     236
                 225     177     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     647     931    1022    1459    1552    1616    1735    1897 
   Size:        647     284      91     437      93      64     119     162

Cuts at:   1897    2122    2552    3718    3931    3986    4071 
   Size:        225     430    1166     213      55      85

  Fragments arranged by size: 

                1166     647     437     430     284     225     213     162
                 119      93      91      85      64      55
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3121    4071 
   Size:       3121     950
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3532    4071 
   Size:       3532     539
 
HaeII r_GCGC'y

Cuts at:      0    1973    3804    4071 
   Size:       1973    1831     267

  Fragments arranged by size: 

                1973    1831     267
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     143     159     175     191     672     704    2202    2234 
   Size:        143      16      16      16     481      32    1498      32

Cuts at:   2234    2698    2730    2743    2775    3208    3240    3448    3480 
   Size:        464      32      13      32     433      32     208      32

Cuts at:   3480    3513    3545    4009    4041    4071 
   Size:         33      32     464      32      30

  Fragments arranged by size: 

                1498     481     464     464     433     208     143      33
                  32      32      32      32      32      32      32      32
                  30      16      16      16      13
 
HincII GTy'rAC

Cuts at:      0    3467    4071 
   Size:       3467     604
 
HindIII A'AGCT_T

Cuts at:      0    1004    1837    2770    4071 
   Size:       1004     833     933    1301

  Fragments arranged by size: 

                1301    1004     933     833
 
KpnI G_GTAC'C

Cuts at:      0     419    4071 
   Size:        419    3652
 
MfeI C'AATT_G

Cuts at:      0    3974    4071 
   Size:       3974      97
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      23     420     884    1767    4071 
   Size:         23     397     464     883    2304

  Fragments arranged by size: 

                2304     883     464     397      23
 
MscI TGG'CCA

Cuts at:      0     793    2332    2417    4071 
   Size:        793    1539      85    1654

  Fragments arranged by size: 

                1654    1539     793      85
 
MslI CAynn'nnrTG

Cuts at:      0    1900    3517    4071 
   Size:       1900    1617     554

  Fragments arranged by size: 

                1900    1617     554
 
MspA1I CmG'CkG

Cuts at:      0     124     896    1875    1992    2524    2634    2757    2818 
   Size:        124     772     979     117     532     110     123      61

Cuts at:   2818    3738    4071 
   Size:        920     333

  Fragments arranged by size: 

                 979     920     772     532     333     124     123     117
                 110      61
 
NaeI GCC'GGC

Cuts at:      0    1798    2089    4071 
   Size:       1798     291    1982

  Fragments arranged by size: 

                1982    1798     291
 
NcoI C'CATG_G

Cuts at:      0      49    4071 
   Size:         49    4022
 
NdeI CA'TA_TG

Cuts at:      0    1376    1574    4071 
   Size:       1376     198    2497

  Fragments arranged by size: 

                2497    1376     198
 
NgoMIV G'CCGG_C

Cuts at:      0    1796    2087    4071 
   Size:       1796     291    1984

  Fragments arranged by size: 

                1984    1796     291
 
NheI G'CTAG_C

Cuts at:      0     696    2709    4071 
   Size:        696    2013    1362

  Fragments arranged by size: 

                2013    1362     696
 
NsiI A_TGCA'T

Cuts at:      0    1740    3643    4071 
   Size:       1740    1903     428

  Fragments arranged by size: 

                1903    1740     428
 
NspI r_CATG'y

Cuts at:      0    1023    2099    3399    3516    3716    3876    4071 
   Size:       1023    1076    1300     117     200     160     195

  Fragments arranged by size: 

                1300    1076    1023     200     195     160     117
 
PasI CC'CwG_GG

Cuts at:      0     828    2880    4071 
   Size:        828    2052    1191

  Fragments arranged by size: 

                2052    1191     828
 
PciI A'CATG_T

Cuts at:      0    1019    4071 
   Size:       1019    3052
 
PfoI T'CCnGG_A

Cuts at:      0     947    3233    4071 
   Size:        947    2286     838

  Fragments arranged by size: 

                2286     947     838
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2974    3006    4071 
   Size:       2974      32    1065

  Fragments arranged by size: 

                2974    1065      32
 
PshAI GACnn'nnGTC

Cuts at:      0     903    4071 
   Size:        903    3168
 
PsiI TTA'TAA

Cuts at:      0      98    4071 
   Size:         98    3973
 
PspXI vC'TCGA_Gb

Cuts at:      0    3470    4071 
   Size:       3470     601
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2951    2983    4071 
   Size:       2951      32    1088

  Fragments arranged by size: 

                2951    1088      32
 
PstI C_TGCA'G

Cuts at:      0     726    1805    3284    3594    4071 
   Size:        726    1079    1479     310     477

  Fragments arranged by size: 

                1479    1079     726     477     310
 
PvuII CAG'CTG

Cuts at:      0     124    1875    2524    2757    2818    3738    4071 
   Size:        124    1751     649     233      61     920     333

  Fragments arranged by size: 

                1751     920     649     333     233     124      61
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2227    2528    4071 
   Size:       2227     301    1543

  Fragments arranged by size: 

                2227    1543     301
 
ScaI AGT'ACT

Cuts at:      0    2783    4071 
   Size:       2783    1288
 
SexAI A'CCwGG_T

Cuts at:      0     759    4071 
   Size:        759    3312
 
SfcI C'TryA_G

Cuts at:      0      83     722    1801    2172    2639    3280    3366    3435 
   Size:         83     639    1079     371     467     641      86      69

Cuts at:   3435    3590    3609    4071 
   Size:        155      19     462

  Fragments arranged by size: 

                1079     641     639     467     462     371     155      86
                  83      69      19
 
SmlI C'TyrA_G

Cuts at:      0    2189    2406    3470    4071 
   Size:       2189     217    1064     601

  Fragments arranged by size: 

                2189    1064     601     217
 
SnaBI TAC'GTA

Cuts at:      0    2151    4071 
   Size:       2151    1920
 
SpeI A'CTAG_T

Cuts at:      0     256     399    4071 
   Size:        256     143    3672

  Fragments arranged by size: 

                3672     256     143
 
SphI G_CATG'C

Cuts at:      0    3516    4071 
   Size:       3516     555
 
SspI AAT'ATT

Cuts at:      0     284    1359    4071 
   Size:        284    1075    2712

  Fragments arranged by size: 

                2712    1075     284
 
StuI AGG'CCT

Cuts at:      0     222    2875    4071 
   Size:        222    2653    1196

  Fragments arranged by size: 

                2653    1196     222
 
StyI C'CwwG_G

Cuts at:      0      49    1817    1898    3304    4071 
   Size:         49    1768      81    1406     767

  Fragments arranged by size: 

                1768    1406     767      81      49
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2444    2710    2931    3745    4036    4071 
   Size:       2444     266     221     814     291      35

  Fragments arranged by size: 

                2444     814     291     266     221      35
 
TatI w'GTAC_w

Cuts at:      0     327    2781    3162    4071 
   Size:        327    2454     381     909

  Fragments arranged by size: 

                2454     909     381     327
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1550    1582    2061    2093    3292    3324    3333    3365 
   Size:       1550      32     479      32    1199      32       9      32

Cuts at:   3365    4071 
   Size:        706

  Fragments arranged by size: 

                1550    1199     706     479      32      32      32      32
                   9
 
Tth111I GACn'n_nGTC

Cuts at:      0     588    4023    4071 
   Size:        588    3435      48

  Fragments arranged by size: 

                3435     588      48
 
XbaI T'CTAG_A

Cuts at:      0    2892    4071 
   Size:       2892    1179
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     825    4071 
   Size:        825    3246
 
XhoI C'TCGA_G

Cuts at:      0    2189    3470    4071 
   Size:       2189    1281     601

  Fragments arranged by size: 

                2189    1281     601
 
XmnI GAAnn'nnTTC

Cuts at:      0    1493    1720    3989    4071 
   Size:       1493     227    2269      82

  Fragments arranged by size: 

                2269    1493     227      82
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI   AflIII     AleI     AlfI     AloI
    AlwNI     ApoI     AvaI     BaeI     BanI    BanII     BbsI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI
    BseYI     BsgI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI     EaeI     EarI  Eco57MI    EcoNI     FalI
    HaeII    Hin4I   HincII  HindIII     KpnI     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NsiI
     NspI     PasI     PciI     PfoI     PpiI    PshAI     PsiI    PspXI
     PsrI     PstI    PvuII     SapI     ScaI    SexAI     SfcI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII     AgeI     AhdI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII    BamHI     BbeI    BbvCI     BcgI
    BmgBI     BplI    BsaBI    BsaHI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BsrBI   BssHII   BstEII  BstZ17I     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI     HpaI     KasI     MluI     NarI     NotI     NruI
     PacI    PflMI     PmeI     PmlI    PpuMI   PspOMI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SrfI     SwaI     XmaI     ZraI
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