Restriction map for pF1KA0456
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

Acc65I      AccII       AflII       AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AviII       AvrII       BanI        BbeI        
BbvCI       BfrI        BglI        BglII       BlnI        
BsiEI       BsiWI       BspDI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
ClaI        CpoI        DraIII      EagI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       FseI        FspI        HpaI        KasI        
KpnI        KspI        MfeI        MluI        MunI        
MvnI        NarI        NdeI        NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       PvuI        RsrII       SacII       
SalI        SapI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspI        StuI        SwaI        XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII           3  GACGTC               GACGT^C
AclI          634  AACGTT               AA^CGTT
AcyI            3  GACGTC               G[GA]^CG[CT]C
AhdI         1966  GACCAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        2522  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2815  CCTGAGG              CC^T[ACGT]AGG
Asp700I      2028  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1966  GACCAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2365  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          224  TGGCCA               TGG^CCA
BamHI        1588  GGATCC               G^GATCC
BbrPI        1987  CACGTG               CAC^GTG
BcgI         2193  CGAGGCCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1532  GTATCC               GTATCC(6/5)
BsaBI        2482  GATATTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI           3  GACGTC               G[GA]^CG[CT]C
BsaI         1275  GGTCTC               GGTCTC(1/5)
BsmBI           5  CGTCTC               CGTCTC(1/5)
BsmI          942  GAATGC               GAATGC(1/-1)
BspMI        1778  ACCTGC               ACCTGC(4/8)
BsrBI        2444  GAGCGG               CCGCTC(-3/-3)
BssSI        2166  CACGAG               CACGAG(-5/-1)
BstXI        2045  CCAGGGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2815  CCTGAGG              CC^T[ACGT]AGG
DraI          415  TTTAAA               TTT^AAA
DrdI         2812  GACCCTGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1966  GACCAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2815  CCTGAGG              CC^T[ACGT]AGG
EcoT22I       782  ATGCAT               ATGCA^T
Hin1I           3  GACGTC               G[GA]^CG[CT]C
MamI         2482  GATATTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         224  TGGCCA               TGG^CCA
MscI          224  TGGCCA               TGG^CCA
NaeI         2303  GCCGGC               GCC^GGC
NgoMIV       2303  GCCGGC               G^CCGGC
NheI         2611  GCTAGC               G^CTAGC
NsiI          782  ATGCAT               ATGCA^T
PflFI        2365  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1987  CACGTG               CAC^GTG
PmlI         1987  CACGTG               CAC^GTG
Psp1406I      634  AACGTT               AA^CGTT
PvuII        2454  CAGCTG               CAG^CTG
SbfI         1779  CCTGCAGG             CCTGCA^GG
SmlI         3001  CTCAAG               C^T[CT][GA]AG
Sse8387I     1779  CCTGCAGG             CCTGCA^GG
Tth111I      2365  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          283  CCAGTCAACTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2028  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI         2065  GTCTAC               GT^[AC][GT]AC
             3189  GTCTAC              
AcsI           16  AAATTC               [GA]^AATT[CT]
             1571  GAATTT              
AflIII        503  ACATGT               A^C[GA][CT]GT
              654  ACATGT              
ApaI         1933  GGGCCC               GGGCC^C
             2058  GGGCCC              
ApoI           16  AAATTC               [GA]^AATT[CT]
             1571  GAATTT              
AvaI         2231  CCCGAG               C^[CT]CG[GA]G
             3010  CCCGAG              
BclI          828  TGATCA               T^GATCA
             2000  TGATCA              
BlpI           76  GCTCAGC              GC^T[ACGT]AGC
             2568  GCTGAGC             
Bpu1102I       76  GCTCAGC              GC^T[ACGT]AGC
             2568  GCTGAGC             
BsaAI        1987  CACGTG               [CT]AC^GT[GA]
             2215  TACGTG              
BsgI         1795  CTGCAC               GTGCAG(16/14)
             2986  CTGCAC              
BsoBI        2231  CCCGAG               C^[CT]CG[GA]G
             3010  CCCGAG              
BspLU11I      503  ACATGT               A^CATGT
              654  ACATGT              
BsrFI         599  ACCGGC               [GA]^CCGG[CT]
             2303  GCCGGC              
BstAPI        129  GCAACTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              775  GCAACCAATGC         
CelII          76  GCTCAGC              GC^T[ACGT]AGC
             2568  GCTGAGC             
Cfr10I        599  ACCGGC               [GA]^CCGG[CT]
             2303  GCCGGC              
FbaI          828  TGATCA               T^GATCA
             2000  TGATCA              
HaeII         980  AGCGCC               [GA]GCGC^[CT]
             3031  AGCGCC              
HincII        286  GTCAAC               GT[CT]^[GA]AC
             3166  GTCAAC              
HindII        286  GTCAAC               GT[CT]^[GA]AC
             3166  GTCAAC              
HindIII      1696  AAGCTT               A^AGCTT
             2787  AAGCTT              
NspI          503  ACATGT               [GA]CATG^[CT]
              654  ACATGT              
PflMI        1665  CCATGACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2521  CCAGAATCTGG         
SexAI         311  ACCAGGT              A^CC[AT]GGT
             1967  ACCAGGT             
Van91I       1665  CCATGACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2521  CCAGAATCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccIII      AciI        AfaI        AluI        AlwI        
AspHI       AvaII       BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BsaWI       BseAI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
Bsp1407I    BspEI       BspHI       BsrDI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MroI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com