Restriction map for pF1KA0456
 (Linear) (Six-base) MAPSORT of: KIAA0456.seq  Check: 3932  from: 1  to: 3213



KIAA0456.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AatII G_ACGT'C

Cuts at:      0       7    3213 
   Size:          7    3206
 
AccI GT'mk_AC

Cuts at:      0    2066    3190    3213 
   Size:       2066    1124      23

  Fragments arranged by size: 

                2066    1124      23
 
AclI AA'CG_TT

Cuts at:      0     635    3213 
   Size:        635    2578
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     751    1714    2444    2749    3213 
   Size:        751     963     730     305     464

  Fragments arranged by size: 

                 963     751     730     464     305
 
AflIII A'CryG_T

Cuts at:      0     503     654    3213 
   Size:        503     151    2559

  Fragments arranged by size: 

                2559     503     151
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1971    3213 
   Size:       1971    1242
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2527    3213 
   Size:       2527     686
 
ApaI G_GGCC'C

Cuts at:      0    1937    2062    3213 
   Size:       1937     125    1151

  Fragments arranged by size: 

                1937    1151     125
 
ApoI r'AATT_y

Cuts at:      0      16    1571    3213 
   Size:         16    1555    1642

  Fragments arranged by size: 

                1642    1555      16
 
AvaI C'yCGr_G

Cuts at:      0    2231    3010    3213 
   Size:       2231     779     203

  Fragments arranged by size: 

                2231     779     203
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      44      77    3213 
   Size:         44      33    3136

  Fragments arranged by size: 

                3136      44      33
 
BamHI G'GATC_C

Cuts at:      0    1588    3213 
   Size:       1588    1625
 
BanII G_rGCy'C

Cuts at:      0     479     670     873    1794    1937    2062    2189    2936 
   Size:        479     191     203     921     143     125     127     747

Cuts at:   2936    2975    3017    3213 
   Size:         39      42     196

  Fragments arranged by size: 

                 921     747     479     203     196     191     143     127
                 125      42      39
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     414     499     783     999    1171    2138    2767    3126 
   Size:        414      85     284     216     172     967     629     359

Cuts at:   3126    3213 
   Size:         87

  Fragments arranged by size: 

                 967     629     414     359     284     216     172      87
                  85
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2182    2216    3213 
   Size:       2182      34     997

  Fragments arranged by size: 

                2182     997      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1543    3213 
   Size:       1543    1670
 
BclI T'GATC_A

Cuts at:      0     828    2000    3213 
   Size:        828    1172    1213

  Fragments arranged by size: 

                1213    1172     828
 
BfrBI ATG'CAT

Cuts at:      0     784    3213 
   Size:        784    2429
 
BlpI GC'TnA_GC

Cuts at:      0      77    2569    3213 
   Size:         77    2492     644

  Fragments arranged by size: 

                2492     644      77
 
Bme1580I G_kGCm'C

Cuts at:      0    1074    1937    2062    2693    3213 
   Size:       1074     863     125     631     520

  Fragments arranged by size: 

                1074     863     631     520     125
 
BmgBI CAC'GTC

Cuts at:      0    2913    2966    3213 
   Size:       2913      53     247

  Fragments arranged by size: 

                2913     247      53
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2456    2944    3213 
   Size:       2456     488     269

  Fragments arranged by size: 

                2456     488     269
 
BmtI G_CTAG'C

Cuts at:      0    2615    3213 
   Size:       2615     598
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     498    3213 
   Size:        498    2715
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     220     266    1393    1482    2074    2961    3213 
   Size:        130      90      46    1127      89     592     887     252

  Fragments arranged by size: 

                1127     887     592     252     130      90      89      46
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    2828    3213 
   Size:       2828     385
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2986    3213 
   Size:       2986     227
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1281    3213 
   Size:       1281    1932
 
BsaAI yAC'GTr

Cuts at:      0    1989    2217    3213 
   Size:       1989     228     996

  Fragments arranged by size: 

                1989     996     228
 
BsaBI GATnn'nnATC

Cuts at:      0    2486    3213 
   Size:       2486     727
 
BsaHI Gr'CG_yC

Cuts at:      0       4    3213 
   Size:          4    3209
 
BsaWI w'CCGG_w

Cuts at:      0     659    1535    1802    2537    2756    3213 
   Size:        659     876     267     735     219     457

  Fragments arranged by size: 

                 876     735     659     457     267     219
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1234    1264    2578    2608    3213 
   Size:       1234      30    1314      30     605

  Fragments arranged by size: 

                1314    1234     605      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     292     577     661     678    1329    1646    2090    2580 
   Size:        292     285      84      17     651     317     444     490

Cuts at:   2580    2583    2989    3213 
   Size:          3     406     224

  Fragments arranged by size: 

                 651     490     444     406     317     292     285     224
                  84      17       3
 
BseYI C'CCAG_C

Cuts at:      0    1061    1073    1300    1648    2630    2993    3043    3213 
   Size:       1061      12     227     348     982     363      50     170

  Fragments arranged by size: 

                1061     982     363     348     227     170      50      12
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1780    2971    3213 
   Size:       1780    1191     242

  Fragments arranged by size: 

                1780    1191     242
 
BsiHKAI G_wGCw'C

Cuts at:      0      78     479     531     670    1794    2162    3213 
   Size:         78     401      52     139    1124     368    1051

  Fragments arranged by size: 

                1124    1051     401     368     139      78      52
 
BsmI GAATG_Cn'

Cuts at:      0     948    3213 
   Size:        948    2265
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      11    3213 
   Size:         11    3202
 
Bsp1286I G_dGCh'C

Cuts at:      0      78     479     531     670     873    1074    1794    1937 
   Size:         78     401      52     139     203     201     720     143

Cuts at:   1937    2062    2162    2189    2693    2936    2975    3017    3213 
   Size:        125     100      27     504     243      39      42     196

  Fragments arranged by size: 

                 720     504     401     243     203     201     196     143
                 139     125     100      78      52      42      39      27
            
 
BspEI T'CCGG_A

Cuts at:      0     659    1802    2537    2756    3213 
   Size:        659    1143     735     219     457

  Fragments arranged by size: 

                1143     735     659     457     219
 
BspHI T'CATG_A

Cuts at:      0    1289    1746    1844    2639    3213 
   Size:       1289     457      98     795     574

  Fragments arranged by size: 

                1289     795     574     457      98
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1787    3213 
   Size:       1787    1426
 
BsrBI CCG'CTC

Cuts at:      0    2446    3213 
   Size:       2446     767
 
BsrDI GCAATG_nn'

Cuts at:      0    1304    2198    2822    3213 
   Size:       1304     894     624     391

  Fragments arranged by size: 

                1304     894     624     391
 
BsrFI r'CCGG_y

Cuts at:      0     599    2303    3213 
   Size:        599    1704     910

  Fragments arranged by size: 

                1704     910     599
 
BsrGI T'GTAC_A

Cuts at:      0     384     506     995    3213 
   Size:        384     122     489    2218

  Fragments arranged by size: 

                2218     489     384     122
 
BssSI C'ACGA_G

Cuts at:      0    2166    3213 
   Size:       2166    1047
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     135     781    3213 
   Size:        135     646    2432

  Fragments arranged by size: 

                2432     646     135
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2052    3213 
   Size:       2052    1161
 
BstYI r'GATC_y

Cuts at:      0      68    1588    2753    3213 
   Size:         68    1520    1165     460

  Fragments arranged by size: 

                1520    1165     460      68
 
Bsu36I CC'TnA_GG

Cuts at:      0    2816    3213 
   Size:       2816     397
 
BtgI C'CryG_G

Cuts at:      0     626    1253    1271    2562    2807    3213 
   Size:        626     627      18    1291     245     406

  Fragments arranged by size: 

                1291     627     626     406     245      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2186    3036    3213 
   Size:       2186     850     177

  Fragments arranged by size: 

                2186     850     177
 
BtsI GCAGTG_nn'

Cuts at:      0     118    2898    3213 
   Size:        118    2780     315

  Fragments arranged by size: 

                2780     315     118
 
DraI TTT'AAA

Cuts at:      0     417    3213 
   Size:        417    2796
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2818    3213 
   Size:       2818     395
 
EaeI y'GGCC_r

Cuts at:      0     224     435     629    3213 
   Size:        224     211     194    2584

  Fragments arranged by size: 

                2584     224     211     194
 
EarI CTCTTCn'nnn_

Cuts at:      0    1148    1732    1886    3213 
   Size:       1148     584     154    1327

  Fragments arranged by size: 

                1327    1148     584     154
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     565    3213 
   Size:        565    2648
 
EcoICRI GAG'CTC

Cuts at:      0     477     668    1792    3213 
   Size:        477     191    1124    1421

  Fragments arranged by size: 

                1421    1124     477     191
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     220     266     751    1393    1482    1714    2074 
   Size:        130      90      46     485     642      89     232     360

Cuts at:   2074    2444    2749    2961    3213 
   Size:        370     305     212     252

  Fragments arranged by size: 

                 642     485     370     360     305     252     232     212
                 130      90      89      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     141     465    1638    1934    2058    2059    3006    3213 
   Size:        141     324    1173     296     124       1     947     207

  Fragments arranged by size: 

                1173     947     324     296     207     141     124       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1719    1758    3213 
   Size:       1719      39    1455

  Fragments arranged by size: 

                1719    1455      39
 
HaeII r_GCGC'y

Cuts at:      0     984    3035    3213 
   Size:        984    2051     178

  Fragments arranged by size: 

                2051     984     178
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      60      92    1957    1989    2578    2610    3213 
   Size:         60      32    1865      32     589      32     603

  Fragments arranged by size: 

                1865     603     589      60      32      32      32
 
HincII GTy'rAC

Cuts at:      0     288    3168    3213 
   Size:        288    2880      45

  Fragments arranged by size: 

                2880     288      45
 
HindIII A'AGCT_T

Cuts at:      0    1696    2787    3213 
   Size:       1696    1091     426

  Fragments arranged by size: 

                1696    1091     426
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2309    3213 
   Size:       2309     904
 
MscI TGG'CCA

Cuts at:      0     226    3213 
   Size:        226    2987
 
MslI CAynn'nnrTG

Cuts at:      0     991    1357    1991    3213 
   Size:        991     366     634    1222

  Fragments arranged by size: 

                1222     991     634     366
 
MspA1I CmG'CkG

Cuts at:      0    1446    2277    2456    2773    3213 
   Size:       1446     831     179     317     440

  Fragments arranged by size: 

                1446     831     440     317     179
 
NaeI GCC'GGC

Cuts at:      0    2305    3213 
   Size:       2305     908
 
NcoI C'CATG_G

Cuts at:      0    1253    2562    2807    3213 
   Size:       1253    1309     245     406

  Fragments arranged by size: 

                1309    1253     406     245
 
NgoMIV G'CCGG_C

Cuts at:      0    2303    3213 
   Size:       2303     910
 
NheI G'CTAG_C

Cuts at:      0    2611    3213 
   Size:       2611     602
 
NsiI A_TGCA'T

Cuts at:      0     786    3213 
   Size:        786    2427
 
NspI r_CATG'y

Cuts at:      0     507     658    3213 
   Size:        507     151    2555

  Fragments arranged by size: 

                2555     507     151
 
PasI CC'CwG_GG

Cuts at:      0    1431    2045    2599    3213 
   Size:       1431     614     554     614

  Fragments arranged by size: 

                1431     614     614     554
 
PciI A'CATG_T

Cuts at:      0     503     654    3213 
   Size:        503     151    2559

  Fragments arranged by size: 

                2559     503     151
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1671    2527    3213 
   Size:       1671     856     686

  Fragments arranged by size: 

                1671     856     686
 
PfoI T'CCnGG_A

Cuts at:      0     146    1193    3213 
   Size:        146    1047    2020

  Fragments arranged by size: 

                2020    1047     146
 
PmlI CAC'GTG

Cuts at:      0    1989    3213 
   Size:       1989    1224
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1709    1741    3213 
   Size:       1709      32    1472

  Fragments arranged by size: 

                1709    1472      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     141     465    1638    3006    3213 
   Size:        141     324    1173    1368     207

  Fragments arranged by size: 

                1368    1173     324     207     141
 
PspOMI G'GGCC_C

Cuts at:      0    1933    2058    3213 
   Size:       1933     125    1155

  Fragments arranged by size: 

                1933    1155     125
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     463     495    3213 
   Size:        463      32    2718

  Fragments arranged by size: 

                2718     463      32
 
PstI C_TGCA'G

Cuts at:      0    1121    1424    1784    3213 
   Size:       1121     303     360    1429

  Fragments arranged by size: 

                1429    1121     360     303
 
PvuII CAG'CTG

Cuts at:      0    2456    3213 
   Size:       2456     757
 
SacI G_AGCT'C

Cuts at:      0     479     670    1794    3213 
   Size:        479     191    1124    1419

  Fragments arranged by size: 

                1419    1124     479     191
 
SbfI CC_TGCA'GG

Cuts at:      0    1784    3213 
   Size:       1784    1429
 
ScaI AGT'ACT

Cuts at:      0     757     799    3039    3213 
   Size:        757      42    2240     174

  Fragments arranged by size: 

                2240     757     174      42
 
SexAI A'CCwGG_T

Cuts at:      0     311    1967    3213 
   Size:        311    1656    1246

  Fragments arranged by size: 

                1656    1246     311
 
SfcI C'TryA_G

Cuts at:      0    1117    1420    1558    1780    2067    3213 
   Size:       1117     303     138     222     287    1146

  Fragments arranged by size: 

                1146    1117     303     287     222     138
 
SmlI C'TyrA_G

Cuts at:      0    3001    3213 
   Size:       3001     212
 
StyI C'CwwG_G

Cuts at:      0     242    1253    1733    2562    2807    2927    3213 
   Size:        242    1011     480     829     245     120     286

  Fragments arranged by size: 

                1011     829     480     286     245     242     120
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3007    3213 
   Size:       3007     206
 
TatI w'GTAC_w

Cuts at:      0     384     506     713     755     797     995    1241    2339 
   Size:        384     122     207      42      42     198     246    1098

Cuts at:   2339    3037    3205    3213 
   Size:        698     168       8

  Fragments arranged by size: 

                1098     698     384     246     207     198     168     122
                  42      42       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      97     129    1580    1612    3213 
   Size:         97      32    1451      32    1601

  Fragments arranged by size: 

                1601    1451      97      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2368    3213 
   Size:       2368     845
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     290    3213 
   Size:        290    2923
 
XmnI GAAnn'nnTTC

Cuts at:      0    2032    3213 
   Size:       2032    1181
 
ZraI GAC'GTC

Cuts at:      0       5    3213 
   Size:          5    3208
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI   AflIII     AhdI    AlwNI     ApaI
     ApoI     AvaI     BaeI    BamHI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI
    HaeII    Hin4I   HincII  HindIII     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NheI     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PmlI     PpiI    PpuMI   PspOMI     PsrI     PstI
    PvuII     SacI     SbfI     ScaI    SexAI     SfcI     SmlI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AfeI    AflII     AgeI     AleI     AlfI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BanI     BbeI    BbvCI
     BglI    BglII    BsiEI    BsiWI   BssHII    BstBI   BstEII  BstZ17I
     ClaI    CspCI   DraIII     EagI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI     NarI
     NdeI     NotI     NruI     PacI     PmeI    PshAI     PsiI    PspXI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     XbaI     XhoI     XmaI
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