Restriction map for pF1KA0400
 (Linear) (Six-base) MAPSORT of: KIAA0400.seq  Check: 1164  from: 1  to: 3018



KIAA0400.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AatII G_ACGT'C

Cuts at:      0    1304    3018 
   Size:       1304    1714
 
AccI GT'mk_AC

Cuts at:      0     745    1811    3018 
   Size:        745    1066    1207

  Fragments arranged by size: 

                1207    1066     745
 
Acc65I G'GTAC_C

Cuts at:      0    1018    3018 
   Size:       1018    2000
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     412     628     634    1157    2176    3018 
   Size:        316      96     216       6     523    1019     842

  Fragments arranged by size: 

                1019     842     523     316     216      96       6
 
AfeI AGC'GCT

Cuts at:      0    2852    3018 
   Size:       2852     166
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1888    2810    3018 
   Size:       1888     922     208

  Fragments arranged by size: 

                1888     922     208
 
AlwNI CAG_nnn'CTG

Cuts at:      0     234     657    1425    3018 
   Size:        234     423     768    1593

  Fragments arranged by size: 

                1593     768     423     234
 
ApaLI G'TGCA_C

Cuts at:      0    3001    3018 
   Size:       3001      17
 
ApoI r'AATT_y

Cuts at:      0      25     857    3018 
   Size:         25     832    2161

  Fragments arranged by size: 

                2161     832      25
 
AvrII C'CTAG_G

Cuts at:      0    2806    3018 
   Size:       2806     212
 
BamHI G'GATC_C

Cuts at:      0     953    3018 
   Size:        953    2065
 
BanI G'GyrC_C

Cuts at:      0      57    1018    1320    3018 
   Size:         57     961     302    1698

  Fragments arranged by size: 

                1698     961     302      57
 
BanII G_rGCy'C

Cuts at:      0    2182    2295    2888    3018 
   Size:       2182     113     593     130

  Fragments arranged by size: 

                2182     593     130     113
 
BbeI G_GCGC'C

Cuts at:      0      61    1324    3018 
   Size:         61    1263    1694

  Fragments arranged by size: 

                1694    1263      61
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1075    2093    2156    2422    3018 
   Size:       1075    1018      63     266     596

  Fragments arranged by size: 

                1075    1018     596     266      63
 
BbvCI CC'TCA_GC

Cuts at:      0    1520    3018 
   Size:       1520    1498
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1540    1574    3018 
   Size:       1540      34    1444

  Fragments arranged by size: 

                1540    1444      34
 
BclI T'GATC_A

Cuts at:      0     659    2909    3018 
   Size:        659    2250     109

  Fragments arranged by size: 

                2250     659     109
 
BfrBI ATG'CAT

Cuts at:      0    1207    3018 
   Size:       1207    1811
 
BglII A'GATC_T

Cuts at:      0      11    3018 
   Size:         11    3007
 
Bme1580I G_kGCm'C

Cuts at:      0    3005    3018 
   Size:       3005      13
 
BmgBI CAC'GTC

Cuts at:      0     135    3018 
   Size:        135    2883
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1450    2126    3018 
   Size:       1450     676     892

  Fragments arranged by size: 

                1450     892     676
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1287    3018 
   Size:       1287    1731
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     256    1250    3018 
   Size:         54     202     994    1768

  Fragments arranged by size: 

                1768     994     202      54
 
Bpu10I CC'TnA_GC

Cuts at:      0    1520    2837    3018 
   Size:       1520    1317     181

  Fragments arranged by size: 

                1520    1317     181
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1894    1975    2598    3018 
   Size:       1894      81     623     420

  Fragments arranged by size: 

                1894     623     420      81
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      31    3018 
   Size:         31    2987
 
BsaAI yAC'GTr

Cuts at:      0     204    3018 
   Size:        204    2814
 
BsaBI GATnn'nnATC

Cuts at:      0    2963    3018 
   Size:       2963      55
 
BsaHI Gr'CG_yC

Cuts at:      0      58    1301    1321    2557    3018 
   Size:         58    1243      20    1236     461

  Fragments arranged by size: 

                1243    1236     461      58      20
 
BsaWI w'CCGG_w

Cuts at:      0     140    1378    3018 
   Size:        140    1238    1640

  Fragments arranged by size: 

                1640    1238     140
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28     352     382    1075    1105    3018 
   Size:         28     324      30     693      30    1913

  Fragments arranged by size: 

                1913     693     324      30      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     136    1096    1611    2023    2385    2941    3018 
   Size:        136     960     515     412     362     556      77

  Fragments arranged by size: 

                 960     556     515     412     362     136      77
 
BseYI C'CCAG_C

Cuts at:      0    1395    2175    2263    2321    2335    2560    2650    3018 
   Size:       1395     780      88      58      14     225      90     368

  Fragments arranged by size: 

                1395     780     368     225      90      88      58      14
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1300    1858    2780    3018 
   Size:       1300     558     922     238

  Fragments arranged by size: 

                1300     922     558     238
 
BsiHKAI G_wGCw'C

Cuts at:      0     871    2003    2888    3005    3018 
   Size:        871    1132     885     117      13

  Fragments arranged by size: 

                1132     885     871     117      13
 
BsmI GAATG_Cn'

Cuts at:      0    1485    1563    2983    3018 
   Size:       1485      78    1420      35

  Fragments arranged by size: 

                1485    1420      78      35
 
Bsp1286I G_dGCh'C

Cuts at:      0     871    2003    2182    2295    2888    3005    3018 
   Size:        871    1132     179     113     593     117      13

  Fragments arranged by size: 

                1132     871     593     179     117     113      13
 
BspEI T'CCGG_A

Cuts at:      0    1378    3018 
   Size:       1378    1640
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     822    1058    1066    1372    3018 
   Size:        822     236       8     306    1646

  Fragments arranged by size: 

                1646     822     306     236       8
 
BsrBI CCG'CTC

Cuts at:      0     541     921    3018 
   Size:        541     380    2097

  Fragments arranged by size: 

                2097     541     380
 
BsrDI GCAATG_nn'

Cuts at:      0     826    1302    1976    3018 
   Size:        826     476     674    1042

  Fragments arranged by size: 

                1042     826     674     476
 
BsrFI r'CCGG_y

Cuts at:      0    1028    2141    2153    2447    3018 
   Size:       1028    1113      12     294     571

  Fragments arranged by size: 

                1113    1028     571     294      12
 
BssSI C'ACGA_G

Cuts at:      0    1996    3018 
   Size:       1996    1022
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2233    3018 
   Size:       2233     785
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1350    2568    2778    2959    3018 
   Size:       1350    1218     210     181      59

  Fragments arranged by size: 

                1350    1218     210     181      59
 
BstYI r'GATC_y

Cuts at:      0      11     420     750     953    1326    1710    1780    2466 
   Size:         11     409     330     203     373     384      70     686

Cuts at:   2466    2609    2736    2964    3018 
   Size:        143     127     228      54

  Fragments arranged by size: 

                 686     409     384     373     330     228     203     143
                 127      70      54      11
 
Bsu36I CC'TnA_GG

Cuts at:      0     893    1079    3018 
   Size:        893     186    1939

  Fragments arranged by size: 

                1939     893     186
 
BtgI C'CryG_G

Cuts at:      0      62     510     710    1014    1784    2365    3018 
   Size:         62     448     200     304     770     581     653

  Fragments arranged by size: 

                 770     653     581     448     304     200      62
 
BtsI GCAGTG_nn'

Cuts at:      0     100    3018 
   Size:        100    2918
 
DraI TTT'AAA

Cuts at:      0    1199    3018 
   Size:       1199    1819
 
DraIII CAC_nnn'GTG

Cuts at:      0    2411    3018 
   Size:       2411     607
 
EaeI y'GGCC_r

Cuts at:      0      32     113    1733    2213    2949    3018 
   Size:         32      81    1620     480     736      69

  Fragments arranged by size: 

                1620     736     480      81      69      32
 
EarI CTCTTCn'nnn_

Cuts at:      0     209     548     937    1805    2504    3018 
   Size:        209     339     389     868     699     514

  Fragments arranged by size: 

                 868     699     514     389     339     209
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2459    3018 
   Size:       2459     559
 
EcoICRI GAG'CTC

Cuts at:      0    2886    3018 
   Size:       2886     132
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     256     316     412     628     634    1157    1250 
   Size:         54     202      60      96     216       6     523      93

Cuts at:   1250    2176    3018 
   Size:        926     842

  Fragments arranged by size: 

                 926     842     523     216     202      96      93      60
                  54       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2236    3018 
   Size:       2236     782
 
EcoO109I rG'GnC_Cy

Cuts at:      0     399    2624    2728    2761    3018 
   Size:        399    2225     104      33     257

  Fragments arranged by size: 

                2225     399     257     104      33
 
EcoRI G'AATT_C

Cuts at:      0      25    3018 
   Size:         25    2993
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1110    3018 
   Size:       1110    1908
 
HaeII r_GCGC'y

Cuts at:      0      61     576    1324    1989    2351    2854    3018 
   Size:         61     515     748     665     362     503     164

  Fragments arranged by size: 

                 748     665     515     503     362     164      61
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30    1780    1812    3018 
   Size:         30    1750      32    1206

  Fragments arranged by size: 

                1750    1206      32      30
 
HincII GTy'rAC

Cuts at:      0     323     615    3018 
   Size:        323     292    2403

  Fragments arranged by size: 

                2403     323     292
 
HindIII A'AGCT_T

Cuts at:      0     443    1193    1636    1691    3018 
   Size:        443     750     443      55    1327

  Fragments arranged by size: 

                1327     750     443     443      55
 
KasI G'GCGC_C

Cuts at:      0      57    1320    3018 
   Size:         57    1263    1698

  Fragments arranged by size: 

                1698    1263      57
 
KpnI G_GTAC'C

Cuts at:      0    1022    3018 
   Size:       1022    1996
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       2    2207    3018 
   Size:          2    2205     811

  Fragments arranged by size: 

                2205     811       2
 
MscI TGG'CCA

Cuts at:      0    1735    2215    2951    3018 
   Size:       1735     480     736      67

  Fragments arranged by size: 

                1735     736     480      67
 
MslI CAynn'nnrTG

Cuts at:      0    2957    3018 
   Size:       2957      61
 
MspA1I CmG'CkG

Cuts at:      0    1519    1971    2175    2476    2490    3018 
   Size:       1519     452     204     301      14     528

  Fragments arranged by size: 

                1519     528     452     301     204      14
 
NaeI GCC'GGC

Cuts at:      0    2449    3018 
   Size:       2449     569
 
NarI GG'CG_CC

Cuts at:      0      58    1321    3018 
   Size:         58    1263    1697

  Fragments arranged by size: 

                1697    1263      58
 
NcoI C'CATG_G

Cuts at:      0     510    1014    3018 
   Size:        510     504    2004

  Fragments arranged by size: 

                2004     510     504
 
NgoMIV G'CCGG_C

Cuts at:      0    2447    3018 
   Size:       2447     571
 
NsiI A_TGCA'T

Cuts at:      0    1209    3018 
   Size:       1209    1809
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     234    3018 
   Size:        234    2784
 
PfoI T'CCnGG_A

Cuts at:      0    1411    3018 
   Size:       1411    1607
 
PmlI CAC'GTG

Cuts at:      0     204    3018 
   Size:        204    2814
 
PpuMI rG'GwC_Cy

Cuts at:      0     399    2728    2761    3018 
   Size:        399    2329      33     257

  Fragments arranged by size: 

                2329     399     257      33
 
PstI C_TGCA'G

Cuts at:      0    2331    2789    3018 
   Size:       2331     458     229

  Fragments arranged by size: 

                2331     458     229
 
PvuII CAG'CTG

Cuts at:      0    1519    2175    2476    3018 
   Size:       1519     656     301     542

  Fragments arranged by size: 

                1519     656     542     301
 
SacI G_AGCT'C

Cuts at:      0    2888    3018 
   Size:       2888     130
 
SapI GCTCTTCn'nnn_

Cuts at:      0     209    3018 
   Size:        209    2809
 
SfcI C'TryA_G

Cuts at:      0     586    2327    2785    3018 
   Size:        586    1741     458     233

  Fragments arranged by size: 

                1741     586     458     233
 
SfoI GGC'GCC

Cuts at:      0      59    1322    3018 
   Size:         59    1263    1696

  Fragments arranged by size: 

                1696    1263      59
 
SmlI C'TyrA_G

Cuts at:      0    1909    1990    2577    3018 
   Size:       1909      81     587     441

  Fragments arranged by size: 

                1909     587     441      81
 
SspI AAT'ATT

Cuts at:      0    1473    3018 
   Size:       1473    1545
 
StuI AGG'CCT

Cuts at:      0    1933    3018 
   Size:       1933    1085
 
StyI C'CwwG_G

Cuts at:      0     510    1014    2237    2806    3018 
   Size:        510     504    1223     569     212

  Fragments arranged by size: 

                1223     569     510     504     212
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     241     931    1906    2814    3018 
   Size:        241     690     975     908     204

  Fragments arranged by size: 

                 975     908     690     241     204
 
TatI w'GTAC_w

Cuts at:      0     221    1437    1586    3018 
   Size:        221    1216     149    1432

  Fragments arranged by size: 

                1432    1216     221     149
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1075    1107    2923    2955    3018 
   Size:       1075      32    1816      32      63

  Fragments arranged by size: 

                1816    1075      63      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1528    3018 
   Size:       1528    1490
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1331    2029    3018 
   Size:       1331     698     989

  Fragments arranged by size: 

                1331     989     698
 
ZraI GAC'GTC

Cuts at:      0    1302    3018 
   Size:       1302    1716
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AfeI     AlfI    AlwNI    ApaLI
     ApoI    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI    BglII Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI     BtsI     DraI   DraIII     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NsiI    PflMI     PfoI     PmlI
    PpuMI     PstI    PvuII     SacI     SapI     SfcI     SfoI     SmlI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     ZraI

 Enzymes that do not cut: 

     AarI     AclI    AflII   AflIII     AgeI     AhdI     AleI     AloI
     ApaI     AscI     AseI    AsiSI     AvaI     BaeI    BciVI     BglI
     BlpI     BmtI    BsiEI    BsiWI    BsmBI    BspHI    BsrGI   BssHII
    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DrdI     EagI
    EcoRV     FseI     FspI    FspAI     HpaI     MfeI     MluI     NdeI
     NheI     NotI     NruI     NspI     PacI     PasI     PciI     PmeI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI     XbaI
     XhoI     XmaI     XmnI
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