Restriction map for
pF1KA0374
(Circular) (Six-base) MAPSORT of: pF1KA0374.seq Check: 9046 from: 1 to: 4727
pF1KA0374 4727 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 264 1765 264
Size: 1501 3226
Acc65I G'GTAC_C
Cuts at: 1743 1743
Size: 4727
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 589 664 1289 2552 2984 3337 3999 538
Size: 51 75 625 1263 432 353 662 1266
Fragments arranged by size:
1266 1263 662 625 432 353 75 51
AflIII A'CryG_T
Cuts at: 2079 3879 4160 2079
Size: 1800 281 2646
Fragments arranged by size:
2646 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1266 1266
Size: 4727
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1190 1190
Size: 4727
AlwNI CAG_nnn'CTG
Cuts at: 465 1264 2032 3470 465
Size: 799 768 1438 1722
Fragments arranged by size:
1722 1438 799 768
ApaI G_GGCC'C
Cuts at: 169 1093 1313 169
Size: 924 220 3583
Fragments arranged by size:
3583 924 220
ApaLI G'TGCA_C
Cuts at: 3565 3565
Size: 4727
ApoI r'AATT_y
Cuts at: 1731 4080 4336 1731
Size: 2349 256 2122
Fragments arranged by size:
2349 2122 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4727
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 196 1747 196
Size: 1551 3176
BamHI G'GATC_C
Cuts at: 1752 1752
Size: 4727
BanI G'GyrC_C
Cuts at: 328 500 1051 1280 1405 1539 1583 1743 2157
Size: 172 551 229 125 134 44 160 414
Cuts at: 2157 2405 2440 328
Size: 248 35 2615
Fragments arranged by size:
2615 551 414 248 229 172 160 134
125 44 35
BanII G_rGCy'C
Cuts at: 169 1093 1313 1373 1490 1741 2771 169
Size: 924 220 60 117 251 1030 2125
Fragments arranged by size:
2125 1030 924 251 220 117 60
BbeI G_GCGC'C
Cuts at: 1587 2161 2409 1587
Size: 574 248 3905
Fragments arranged by size:
3905 574 248
BbsI GAAGACnn'nnnn_
Cuts at: 577 577
Size: 4727
BbvCI CC'TCA_GC
Cuts at: 189 950 189
Size: 761 3966
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4693
BciVI GTATCCnnnnn_n'
Cuts at: 194 2625 3681 4727 194
Size: 2431 1056 1046 194
Fragments arranged by size:
2431 1056 1046 194
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BglI GCCn_nnn'nGGC
Cuts at: 637 1703 637
Size: 1066 3661
BglII A'GATC_T
Cuts at: 2242 4279 2242
Size: 2037 2690
BlpI GC'TnA_GC
Cuts at: 340 1678 1839 340
Size: 1338 161 3228
Fragments arranged by size:
3228 1338 161
Bme1580I G_kGCm'C
Cuts at: 169 1093 1285 1313 1447 1553 2352 2445 3569
Size: 924 192 28 134 106 799 93 1124
Cuts at: 3569 169
Size: 1327
Fragments arranged by size:
1327 1124 924 799 192 134 106 93
28
BmgBI CAC'GTC
Cuts at: 140 140
Size: 4727
BmrI ACTGGGnnnn_n'
Cuts at: 839 1939 2020 2355 4499 839
Size: 1100 81 335 2144 1067
Fragments arranged by size:
2144 1100 1067 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1080 3094 1080
Size: 2014 2713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 790 921 1246 1521 523
Size: 267 131 325 275 3729
Fragments arranged by size:
3729 325 275 267 131
Bpu10I CC'TnA_GC
Cuts at: 189 950 189
Size: 761 3966
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1903 2782 3496 3794 658
Size: 1245 879 714 298 1591
Fragments arranged by size:
1591 1245 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1054 1310 4431 1054
Size: 256 3121 1350
Fragments arranged by size:
3121 1350 256
BsaAI yAC'GTr
Cuts at: 2080 2080
Size: 4727
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 1584 2158 2406 3108 4604 1584
Size: 574 248 702 1496 1707
Fragments arranged by size:
1707 1496 702 574 248
BsaWI w'CCGG_w
Cuts at: 1916 2437 3115 3526 3673 3947 1916
Size: 521 678 411 147 274 2696
Fragments arranged by size:
2696 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1597 1627 1597
Size: 30 4697
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 177 622 625 1072 1471 1474 1477 177
Size: 445 3 447 399 3 3 3427
Fragments arranged by size:
3427 447 445 399 3 3 3
BseYI C'CCAG_C
Cuts at: 124 632 1577 1693 1698 1715 2152 2910 3575
Size: 508 945 116 5 17 437 758 665
Cuts at: 3575 3983 124
Size: 408 868
Fragments arranged by size:
945 868 758 665 508 437 408 116
17 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 766 1054 1171 1216 1617 441
Size: 325 288 117 45 401 3551
Fragments arranged by size:
3551 401 325 288 117 45
BsiEI CG_ry'CG
Cuts at: 104 1547 2269 2315 3545 104
Size: 1443 722 46 1230 1286
Fragments arranged by size:
1443 1286 1230 722 46
BsiHKAI G_wGCw'C
Cuts at: 1741 2519 2709 3569 1741
Size: 778 190 860 2899
Fragments arranged by size:
2899 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 766 766
Size: 4727
Bsp1286I G_dGCh'C
Cuts at: 169 1093 1285 1313 1373 1447 1490 1553 1741
Size: 924 192 28 60 74 43 63 188
Cuts at: 1741 2352 2445 2519 2709 2771 3569 169
Size: 611 93 74 190 62 798 1327
Fragments arranged by size:
1327 924 798 611 192 190 188 93
74 74 63 62 60 43 28
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 669 1777 1991 2293 2674 669
Size: 1108 214 302 381 2722
Fragments arranged by size:
2722 1108 381 302 214
BsrBI CCG'CTC
Cuts at: 50 860 1574 1673 3019 3073 4557 4722 50
Size: 810 714 99 1346 54 1484 165 55
Fragments arranged by size:
1484 1346 810 714 165 99 55 54
BsrDI GCAATG_nn'
Cuts at: 2639 2639
Size: 4727
BsrFI r'CCGG_y
Cuts at: 533 841 1542 2725 2906 533
Size: 308 701 1183 181 2354
Fragments arranged by size:
2354 1183 701 308 181
BssSI C'ACGA_G
Cuts at: 2998 3706 2998
Size: 708 4019
BstAPI GCAn_nnn'nTGC
Cuts at: 2104 2104
Size: 4727
BstBI TT'CG_AA
Cuts at: 3088 3088
Size: 4727
BstEII G'GTnAC_C
Cuts at: 913 913
Size: 4727
BstXI CCAn_nnnn'nTGG
Cuts at: 1252 1252
Size: 4727
BstYI r'GATC_y
Cuts at: 1 1521 1752 2219 2242 2577 2823 3129 3141
Size: 1520 231 467 23 335 246 306 12
Cuts at: 3141 3227 4279 1
Size: 86 1052 449
Fragments arranged by size:
1520 1052 467 449 335 306 246 231
86 23 12
BtgI C'CryG_G
Cuts at: 432 1380 1448 1472 1553 432
Size: 948 68 24 81 3606
Fragments arranged by size:
3606 948 81 68 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2677 2858 3021 2677
Size: 181 163 4383
Fragments arranged by size:
4383 181 163
BtsI GCAGTG_nn'
Cuts at: 381 429 1337 1970 381
Size: 48 908 633 3138
Fragments arranged by size:
3138 908 633 48
DraI TTT'AAA
Cuts at: 1726 1726
Size: 4727
DraIII CAC_nnn'GTG
Cuts at: 419 624 419
Size: 205 4522
DrdI GACnn_nn'nnGTC
Cuts at: 579 904 2433 3777 579
Size: 325 1529 1344 1529
Fragments arranged by size:
1529 1529 1344 325
EaeI y'GGCC_r
Cuts at: 242 812 1422 1544 2312 2486 2877 2904 4249
Size: 570 610 122 768 174 391 27 1345
Cuts at: 4249 242
Size: 720
Fragments arranged by size:
1345 768 720 610 570 391 174 122
27
EagI C'GGCC_G
Cuts at: 1544 2312 1544
Size: 768 3959
EarI CTCTTCn'nnn_
Cuts at: 339 1448 2750 2960 339
Size: 1109 1302 210 2106
Fragments arranged by size:
2106 1302 1109 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2935 3677 3823 4537 2935
Size: 742 146 714 3125
Fragments arranged by size:
3125 742 714 146
EcoICRI GAG'CTC
Cuts at: 1739 1739
Size: 4727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 538 589 664 790 921 1246 1289 1521
Size: 15 51 75 126 131 325 43 232
Cuts at: 1521 2552 2984 3337 3999 523
Size: 1031 432 353 662 1251
Fragments arranged by size:
1251 1031 662 432 353 325 232 131
126 75 51 43 15
EcoNI CCTnn'n_nnAGG
Cuts at: 646 1268 646
Size: 622 4105
EcoO109I rG'GnC_Cy
Cuts at: 166 848 1090 1309 1362 1866 166
Size: 682 242 219 53 504 3027
Fragments arranged by size:
3027 682 504 242 219 53
EcoRI G'AATT_C
Cuts at: 1731 1731
Size: 4727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4727
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 402 1587 2161 2409 3639 4410 402
Size: 1185 574 248 1230 771 719
Fragments arranged by size:
1230 1185 771 719 574 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1252 1284 1309 1341 2569 2599 2601 2631 1252
Size: 32 25 32 1228 30 2 30 3348
Fragments arranged by size:
3348 1228 32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 1766 1766
Size: 4727
KasI G'GCGC_C
Cuts at: 1583 2157 2405 1583
Size: 574 248 3905
Fragments arranged by size:
3905 574 248
KpnI G_GTAC'C
Cuts at: 1747 1747
Size: 4727
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3486 3670 3486
Size: 184 4543
MscI TGG'CCA
Cuts at: 244 814 1424 2488 244
Size: 570 610 1064 2483
Fragments arranged by size:
2483 1064 610 570
MslI CAynn'nnrTG
Cuts at: 244 793 2716 2843 244
Size: 549 1923 127 2128
Fragments arranged by size:
2128 1923 549 127
MspA1I CmG'CkG
Cuts at: 119 579 636 941 1160 1398 1581 1667 1702
Size: 460 57 305 219 238 183 86 35
Cuts at: 1702 1838 2152 2512 3296 3541 119
Size: 136 314 360 784 245 1305
Fragments arranged by size:
1305 784 460 360 314 305 245 238
219 183 136 86 57 35
NaeI GCC'GGC
Cuts at: 1544 2908 1544
Size: 1364 3363
NarI GG'CG_CC
Cuts at: 1584 2158 2406 1584
Size: 574 248 3905
Fragments arranged by size:
3905 574 248
NcoI C'CATG_G
Cuts at: 432 1448 432
Size: 1016 3711
NgoMIV G'CCGG_C
Cuts at: 1542 2906 1542
Size: 1364 3363
NspI r_CATG'y
Cuts at: 591 1167 1780 2280 3883 591
Size: 576 613 500 1603 1435
Fragments arranged by size:
1603 1435 613 576 500
PciI A'CATG_T
Cuts at: 3879 3879
Size: 4727
PflMI CCAn_nnn'nTGG
Cuts at: 624 624
Size: 4727
PfoI T'CCnGG_A
Cuts at: 539 1223 539
Size: 684 4043
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4695
PpuMI rG'GwC_Cy
Cuts at: 848 848
Size: 4727
PshAI GACnn'nnGTC
Cuts at: 383 383
Size: 4727
PspOMI G'GGCC_C
Cuts at: 165 1089 1309 165
Size: 924 220 3583
Fragments arranged by size:
3583 924 220
PstI C_TGCA'G
Cuts at: 479 731 1774 2459 479
Size: 252 1043 685 2747
Fragments arranged by size:
2747 1043 685 252
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 579 636 1160 1667 2152 2512 579
Size: 57 524 507 485 360 2794
Fragments arranged by size:
2794 524 507 485 360 57
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1741 1741
Size: 4727
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SanDI GG'GwC_CC
Cuts at: 848 848
Size: 4727
SapI GCTCTTCn'nnn_
Cuts at: 2750 2960 2750
Size: 210 4517
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 736 4244 736
Size: 3508 1219
SfcI C'TryA_G
Cuts at: 33 475 727 1770 2455 3423 3614 33
Size: 442 252 1043 685 968 191 1146
Fragments arranged by size:
1146 1043 968 685 442 252 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 637 637
Size: 4727
SfoI GGC'GCC
Cuts at: 1585 2159 2407 1585
Size: 574 248 3905
Fragments arranged by size:
3905 574 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 198 1749 198
Size: 1551 3176
SmlI C'TyrA_G
Cuts at: 673 1882 2797 3511 3773 673
Size: 1209 915 714 262 1627
Fragments arranged by size:
1627 1209 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 591 1167 1780 2280 591
Size: 576 613 500 3038
Fragments arranged by size:
3038 613 576 500
StyI C'CwwG_G
Cuts at: 394 432 815 1448 1861 2215 394
Size: 38 383 633 413 354 2906
Fragments arranged by size:
2906 633 413 383 354 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1336 2310 2443 2902 3111 1336
Size: 974 133 459 209 2952
Fragments arranged by size:
2952 974 459 209 133
TatI w'GTAC_w
Cuts at: 734 4242 734
Size: 3508 1219
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 4695
Tth111I GACn'n_nGTC
Cuts at: 691 2524 691
Size: 1833 2894
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1329 2222 1329
Size: 893 3834
XmaI C'CCGG_G
Cuts at: 196 1747 196
Size: 1551 3176
XmnI GAAnn'nnTTC
Cuts at: 736 736
Size: 4727
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AloI AscI
AvrII BaeI BfrBI BmtI BsiWI BsmI BspEI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PsiI PspXI PsrI SacII SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI