Restriction map for pF1KA0291
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AviII       AvrII       BcgI        BciVI       
BclI        BfrI        BlnI        BsiWI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DrdI        
Eco47III    Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MfeI        MluI        MseI        MunI        NaeI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
ScaI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        SspBI       SspI        SwaI        
TfiI        Tru9I       XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccIII       1494  TCCGGA               T^CCGGA
AhdI          933  GACCCACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2089  GTGCAC               G^TGCAC
ApaLI        2089  GTGCAC               G^TGCAC
AspEI         933  GACCCACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2541  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         607  GGATCC               G^GATCC
BbrPI        1927  CACGTG               CAC^GTG
BbvCI        1138  GCTGAGG              CCTCAGC(-5/-2)
BlpI         2905  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2905  GCTCAGC              GC^T[ACGT]AGC
BsaBI         855  GATCCGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1494  TCCGGA               T^CCGGA
BsmI          513  GCATTC               GAATGC(1/-1)
BspEI        1494  TCCGGA               T^CCGGA
BsrDI         509  CATTGC               GCAATG(2/0)
BstAPI        169  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        2905  GCTCAGC              GC^T[ACGT]AGC
Eam1105I      933  GACCCACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
MamI          855  GATCCGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1494  TCCGGA               T^CCGGA
NheI          106  GCTAGC               G^CTAGC
PflFI        2541  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         474  CCACTCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1927  CACGTG               CAC^GTG
PmlI         1927  CACGTG               CAC^GTG
SbfI         2376  CCTGCAGG             CCTGCA^GG
SexAI         799  ACCAGGT              A^CC[AT]GGT
SfiI         1602  GGCCCTGAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I     2376  CCTGCAGG             CCTGCA^GG
StuI         1610  AGGCCT               AGG^CCT
Tsp509I      2971  AATT                 ^AATT
Tth111I      2541  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        474  CCACTCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BalI          911  TGGCCA               TGG^CCA
             1145  TGGCCA              
BfaI          107  CTAG                 C^TAG
             2578  CTAG                
BglII        1049  AGATCT               A^GATCT
             2159  AGATCT              
BsaAI         709  TACGTG               [CT]AC^GT[GA]
             1927  CACGTG              
BsaWI        1494  TCCGGA               [AT]^CCGG[AT]
             2974  TCCGGT              
BsgI          130  CTGCAC               GTGCAG(16/14)
              283  GTGCAG              
BsmBI         298  GAGACG               CGTCTC(1/5)
             2344  GAGACG              
BspMI         653  ACCTGC               ACCTGC(4/8)
             2910  GCAGGT              
BsrFI         592  ACCGGC               [GA]^CCGG[CT]
             2036  ACCGGC              
BssSI        2092  CACGAG               CACGAG(-5/-1)
             1258  CTCGTG              
Cfr10I        592  ACCGGC               [GA]^CCGG[CT]
             2036  ACCGGC              
DraIII        475  CACTCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
              847  CACAAAGTG           
EarI          832  CTCTTC               CTCTTC(1/4)
             1029  CTCTTC              
EcoNI        1191  CCTGCTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2502  CCTCCTGAAGG         
Ksp632I       832  CTCTTC               CTCTTC(1/4)
             1029  CTCTTC              
MaeI          107  CTAG                 C^TAG
             2578  CTAG                
MluNI         911  TGGCCA               TGG^CCA
             1145  TGGCCA              
MscI          911  TGGCCA               TGG^CCA
             1145  TGGCCA              
NcoI           56  CCATGG               C^CATGG
              914  CCATGG              
SacI         1426  GAGCTC               GAGCT^C
             1456  GAGCTC              
Tsp45I       2410  GTGAC                ^GT[GC]AC
             2657  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbeI        BbsI        
BbvI        BcnI        BglI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrBI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EagI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmlI        StyI        
TaqI        TseI        TspRI       TthHB8I     XcmI        
XhoII       
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