Restriction map for pF1KA0291
 (Linear) (Six-base) MAPSORT of: KIAA0291.seq  Check: 3805  from: 1  to: 3033



KIAA0291.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2901    3033 
   Size:       2901     132
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40    1102    1627    1699    1861    2527    3033 
   Size:         40    1062     525      72     162     666     506

  Fragments arranged by size: 

                1062     666     525     506     162      72      40
 
AhdI GACnn_n'nnGTC

Cuts at:      0     938    3033 
   Size:        938    2095
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1030    1235    3033 
   Size:       1030     205    1798

  Fragments arranged by size: 

                1798    1030     205
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1321    1353    3033 
   Size:       1321      32    1680

  Fragments arranged by size: 

                1680    1321      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     175     291     504    1113    1470    2598    3033 
   Size:        175     116     213     609     357    1128     435

  Fragments arranged by size: 

                1128     609     435     357     213     175     116
 
ApaI G_GGCC'C

Cuts at:      0     196    1810    2010    2448    3033 
   Size:        196    1614     200     438     585

  Fragments arranged by size: 

                1614     585     438     200     196
 
ApaLI G'TGCA_C

Cuts at:      0    2089    3033 
   Size:       2089     944
 
AvaI C'yCGr_G

Cuts at:      0     181     459    1053    1479    1803    2568    2836    3033 
   Size:        181     278     594     426     324     765     268     197

  Fragments arranged by size: 

                 765     594     426     324     278     268     197     181
            
 
BamHI G'GATC_C

Cuts at:      0     607    3033 
   Size:        607    2426
 
BanI G'GyrC_C

Cuts at:      0     796    1057    1813    2519    2832    3033 
   Size:        796     261     756     706     313     201

  Fragments arranged by size: 

                 796     756     706     313     261     201
 
BanII G_rGCy'C

Cuts at:      0     196     460     767    1251    1430    1460    1810    2010 
   Size:        196     264     307     484     179      30     350     200

Cuts at:   2010    2448    3033 
   Size:        438     585

  Fragments arranged by size: 

                 585     484     438     350     307     264     200     196
                 179      30
 
BbeI G_GCGC'C

Cuts at:      0    1817    2523    2836    3033 
   Size:       1817     706     313     197

  Fragments arranged by size: 

                1817     706     313     197
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     595     697     904    2560    3033 
   Size:        595     102     207    1656     473

  Fragments arranged by size: 

                1656     595     473     207     102
 
BbvCI CC'TCA_GC

Cuts at:      0    1139    3033 
   Size:       1139    1894
 
BglI GCCn_nnn'nGGC

Cuts at:      0      59    1010    1609    1618    3033 
   Size:         59     951     599       9    1415

  Fragments arranged by size: 

                1415     951     599      59       9
 
BglII A'GATC_T

Cuts at:      0    1049    2159    3033 
   Size:       1049    1110     874

  Fragments arranged by size: 

                1110    1049     874
 
BlpI GC'TnA_GC

Cuts at:      0    2906    3033 
   Size:       2906     127
 
Bme1580I G_kGCm'C

Cuts at:      0     196     397     523     799     817    1060    1810    2010 
   Size:        196     201     126     276      18     243     750     200

Cuts at:   2010    2093    2448    3033 
   Size:         83     355     585

  Fragments arranged by size: 

                 750     585     355     276     243     201     200     196
                 126      83      18
 
BmgBI CAC'GTC

Cuts at:      0    2112    3033 
   Size:       2112     921
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      83    3033 
   Size:         83    2950
 
BmtI G_CTAG'C

Cuts at:      0     110    3033 
   Size:        110    2923
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2178    3033 
   Size:       2178     855
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33     388    1324    1396    1438    1444    1798    1900 
   Size:         33     355     936      72      42       6     354     102

Cuts at:   1900    1933    2035    2062    2260    2482    2545    2557    2665 
   Size:         33     102      27     198     222      63      12     108

Cuts at:   2665    3033 
   Size:        368

  Fragments arranged by size: 

                 936     368     355     354     222     198     108     102
                 102      72      63      42      33      33      27      12
                   6
 
Bpu10I CC'TnA_GC

Cuts at:      0    1139    2440    2683    3033 
   Size:       1139    1301     243     350

  Fragments arranged by size: 

                1301    1139     350     243
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1180    2134    2716    3033 
   Size:       1180     954     582     317

  Fragments arranged by size: 

                1180     954     582     317
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     835    1018    1118    1364    1909    2533    3033 
   Size:        835     183     100     246     545     624     500

  Fragments arranged by size: 

                 835     624     545     500     246     183     100
 
BsaAI yAC'GTr

Cuts at:      0     711    1929    3033 
   Size:        711    1218    1104

  Fragments arranged by size: 

                1218    1104     711
 
BsaBI GATnn'nnATC

Cuts at:      0     859    3033 
   Size:        859    2174
 
BsaHI Gr'CG_yC

Cuts at:      0    1508    1814    2498    2520    2564    2833    2879    3033 
   Size:       1508     306     684      22      44     269      46     154

  Fragments arranged by size: 

                1508     684     306     269     154      46      44      22
            
 
BsaWI w'CCGG_w

Cuts at:      0    1494    2974    3033 
   Size:       1494    1480      59

  Fragments arranged by size: 

                1494    1480      59
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1339    1369    2455    2485    2530    2560    3033 
   Size:       1339      30    1086      30      45      30     473

  Fragments arranged by size: 

                1339    1086     473      45      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     144     147     972    1246    1321    1324    1329    1363 
   Size:        144       3     825     274      75       3       5      34

Cuts at:   1363    1366    1435    1438    1468    1990    2289    2356    2952 
   Size:          3      69       3      30     522     299      67     596

Cuts at:   2952    3033 
   Size:         81

  Fragments arranged by size: 

                 825     596     522     299     274     144      81      75
                  69      67      34      30       5       3       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0      10     882     943    1000    1817    1884    1984    2009 
   Size:         10     872      61      57     817      67     100      25

Cuts at:   2009    2303    3033 
   Size:        294     730

  Fragments arranged by size: 

                 872     817     730     294     100      67      61      57
                  25      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     304    3033 
   Size:        115     189    2729

  Fragments arranged by size: 

                2729     189     115
 
BsiEI CG_ry'CG

Cuts at:      0      33     167     452    2285    3033 
   Size:         33     134     285    1833     748

  Fragments arranged by size: 

                1833     748     285     134      33
 
BsiHKAI G_wGCw'C

Cuts at:      0    1430    1460    1874    2093    2299    2872    3033 
   Size:       1430      30     414     219     206     573     161

  Fragments arranged by size: 

                1430     573     414     219     206     161      30
 
BsmI GAATG_Cn'

Cuts at:      0     513    3033 
   Size:        513    2520
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     292    2338    3033 
   Size:        292    2046     695

  Fragments arranged by size: 

                2046     695     292
 
Bsp1286I G_dGCh'C

Cuts at:      0     196     397     460     523     767     799     817    1060 
   Size:        196     201      63      63     244      32      18     243

Cuts at:   1060    1251    1430    1460    1810    1874    2010    2093    2299 
   Size:        191     179      30     350      64     136      83     206

Cuts at:   2299    2448    2872    3033 
   Size:        149     424     161

  Fragments arranged by size: 

                 424     350     244     243     206     201     196     191
                 179     161     149     136      83      64      63      63
                  32      30      18
 
BspEI T'CCGG_A

Cuts at:      0    1494    3033 
   Size:       1494    1539
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     662    2901    3033 
   Size:        662    2239     132

  Fragments arranged by size: 

                2239     662     132
 
BsrBI CCG'CTC

Cuts at:      0     249    1536    2196    2613    3033 
   Size:        249    1287     660     417     420

  Fragments arranged by size: 

                1287     660     420     417     249
 
BsrDI GCAATG_nn'

Cuts at:      0     508    3033 
   Size:        508    2525
 
BsrFI r'CCGG_y

Cuts at:      0     592    2036    3033 
   Size:        592    1444     997

  Fragments arranged by size: 

                1444     997     592
 
BssSI C'ACGA_G

Cuts at:      0    1258    2092    3033 
   Size:       1258     834     941

  Fragments arranged by size: 

                1258     941     834
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     175    3033 
   Size:        175    2858
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      57     325     909    1302    3033 
   Size:         57     268     584     393    1731

  Fragments arranged by size: 

                1731     584     393     268      57
 
BstYI r'GATC_y

Cuts at:      0     607    1049    1329    1517    2159    3033 
   Size:        607     442     280     188     642     874

  Fragments arranged by size: 

                 874     642     607     442     280     188
 
BtgI C'CryG_G

Cuts at:      0      33      56     446     479     914     986    1061    3033 
   Size:         33      23     390      33     435      72      75    1972

  Fragments arranged by size: 

                1972     435     390      75      72      33      33      23
            
 
BtsI GCAGTG_nn'

Cuts at:      0     127    1014    1471    1853    3033 
   Size:        127     887     457     382    1180

  Fragments arranged by size: 

                1180     887     457     382     127
 
DraIII CAC_nnn'GTG

Cuts at:      0     480     852    3033 
   Size:        480     372    2181

  Fragments arranged by size: 

                2181     480     372
 
EaeI y'GGCC_r

Cuts at:      0      30     101     164     449     524     911    1145    1391 
   Size:         30      71      63     285      75     387     234     246

Cuts at:   1391    1547    1643    2168    3033 
   Size:        156      96     525     865

  Fragments arranged by size: 

                 865     525     387     285     246     234     156      96
                  75      71      63      30
 
EagI C'GGCC_G

Cuts at:      0      30     164     449    3033 
   Size:         30     134     285    2584

  Fragments arranged by size: 

                2584     285     134      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     838    1035    3033 
   Size:        838     197    1998

  Fragments arranged by size: 

                1998     838     197
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     220    1636    2727    3033 
   Size:        220    1416    1091     306

  Fragments arranged by size: 

                1416    1091     306     220
 
EcoICRI GAG'CTC

Cuts at:      0    1428    1458    3033 
   Size:       1428      30    1575

  Fragments arranged by size: 

                1575    1428      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33      40     388    1102    1324    1396    1438    1444 
   Size:         33       7     348     714     222      72      42       6

Cuts at:   1444    1627    1699    1798    1861    1900    1933    2035    2062 
   Size:        183      72      99      63      39      33     102      27

Cuts at:   2062    2260    2482    2527    2545    2557    2665    3033 
   Size:        198     222      45      18      12     108     368

  Fragments arranged by size: 

                 714     368     348     222     222     198     183     108
                 102      99      72      72      63      45      42      39
                  33      33      27      18      12       7       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1195    2506    3033 
   Size:       1195    1311     527

  Fragments arranged by size: 

                1311    1195     527
 
EcoO109I rG'GnC_Cy

Cuts at:      0     193     651    1483    1602    1782    1944    2289    2373 
   Size:        193     458     832     119     180     162     345      84

Cuts at:   2373    2444    2679    2748    3033 
   Size:         71     235      69     285

  Fragments arranged by size: 

                 832     458     345     285     235     193     180     162
                 119      84      71      69
 
HaeII r_GCGC'y

Cuts at:      0    1817    2070    2523    2808    2836    3033 
   Size:       1817     253     453     285      28     197

  Fragments arranged by size: 

                1817     453     285     253     197      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     460     492     592     599     613     624     631     645 
   Size:        460      32     100       7      14      11       7      14

Cuts at:    645     924     956    1339    1371    1433    1465    2530    2562 
   Size:        279      32     383      32      62      32    1065      32

Cuts at:   2562    2812    2844    3033 
   Size:        250      32     189

  Fragments arranged by size: 

                1065     460     383     279     250     189     100      62
                  32      32      32      32      32      32      14      14
                  11       7       7
 
KasI G'GCGC_C

Cuts at:      0    1813    2519    2832    3033 
   Size:       1813     706     313     201

  Fragments arranged by size: 

                1813     706     313     201
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     635    1319    2165    2774    3033 
   Size:        635     684     846     609     259

  Fragments arranged by size: 

                 846     684     635     609     259
 
MscI TGG'CCA

Cuts at:      0     913    1147    3033 
   Size:        913     234    1886

  Fragments arranged by size: 

                1886     913     234
 
MslI CAynn'nnrTG

Cuts at:      0     919    1864    1927    2314    3033 
   Size:        919     945      63     387     719

  Fragments arranged by size: 

                 945     919     719     387      63
 
MspA1I CmG'CkG

Cuts at:      0      14     172     537     626    1110    1302    1416    1878 
   Size:         14     158     365      89     484     192     114     462

Cuts at:   1878    1995    2013    2205    2862    3033 
   Size:        117      18     192     657     171

  Fragments arranged by size: 

                 657     484     462     365     192     192     171     158
                 117     114      89      18      14
 
NarI GG'CG_CC

Cuts at:      0    1814    2520    2833    3033 
   Size:       1814     706     313     200

  Fragments arranged by size: 

                1814     706     313     200
 
NcoI C'CATG_G

Cuts at:      0      56     914    3033 
   Size:         56     858    2119

  Fragments arranged by size: 

                2119     858      56
 
NheI G'CTAG_C

Cuts at:      0     106    3033 
   Size:        106    2927
 
PasI CC'CwG_GG

Cuts at:      0    2217    2665    3033 
   Size:       2217     448     368

  Fragments arranged by size: 

                2217     448     368
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     480    3033 
   Size:        480    2553
 
PfoI T'CCnGG_A

Cuts at:      0    2548    3033 
   Size:       2548     485
 
PmlI CAC'GTG

Cuts at:      0    1929    3033 
   Size:       1929    1104
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     289     321    3033 
   Size:        289      32    2712

  Fragments arranged by size: 

                2712     289      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     651    1483    1782    2679    3033 
   Size:        651     832     299     897     354

  Fragments arranged by size: 

                 897     832     651     354     299
 
PspOMI G'GGCC_C

Cuts at:      0     192    1806    2006    2444    3033 
   Size:        192    1614     200     438     589

  Fragments arranged by size: 

                1614     589     438     200     192
 
PstI C_TGCA'G

Cuts at:      0    1244    1337    1415    1964    1994    2051    2204    2381 
   Size:       1244      93      78     549      30      57     153     177

Cuts at:   2381    2855    3033 
   Size:        474     178

  Fragments arranged by size: 

                1244     549     474     178     177     153      93      78
                  57      30
 
PvuII CAG'CTG

Cuts at:      0     172    1110    1302    1416    1878    2013    2862    3033 
   Size:        172     938     192     114     462     135     849     171

  Fragments arranged by size: 

                 938     849     462     192     172     171     135     114
            
 
SacI G_AGCT'C

Cuts at:      0    1430    1460    3033 
   Size:       1430      30    1573

  Fragments arranged by size: 

                1573    1430      30
 
SanDI GG'GwC_CC

Cuts at:      0    1483    3033 
   Size:       1483    1550
 
SbfI CC_TGCA'GG

Cuts at:      0    2381    3033 
   Size:       2381     652
 
SexAI A'CCwGG_T

Cuts at:      0     799    3033 
   Size:        799    2234
 
SfcI C'TryA_G

Cuts at:      0    1240    1333    1411    1960    1990    2047    2200    2377 
   Size:       1240      93      78     549      30      57     153     177

Cuts at:   2377    2851    3033 
   Size:        474     182

  Fragments arranged by size: 

                1240     549     474     182     177     153      93      78
                  57      30
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1609    3033 
   Size:       1609    1424
 
SfoI GGC'GCC

Cuts at:      0    1815    2521    2834    3033 
   Size:       1815     706     313     199

  Fragments arranged by size: 

                1815     706     313     199
 
SmlI C'TyrA_G

Cuts at:      0    1195    2149    2731    3033 
   Size:       1195     954     582     302

  Fragments arranged by size: 

                1195     954     582     302
 
StuI AGG'CCT

Cuts at:      0    1612    3033 
   Size:       1612    1421
 
StyI C'CwwG_G

Cuts at:      0      56     113     197     731     768     887     914    1148 
   Size:         56      57      84     534      37     119      27     234

Cuts at:   1148    1364    3033 
   Size:        216    1669

  Fragments arranged by size: 

                1669     534     234     216     119      84      57      56
                  37      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     246     834     907    1517    2326    2446    3033 
   Size:        246     588      73     610     809     120     587

  Fragments arranged by size: 

                 809     610     588     587     246     120      73
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1756    1788    2455    2487    3033 
   Size:       1756      32     667      32     546

  Fragments arranged by size: 

                1756     667     546      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2544    3033 
   Size:       2544     489
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1371    1570    1710    1908    3033 
   Size:       1371     199     140     198    1125

  Fragments arranged by size: 

                1371    1125     199     198     140
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstXI
    BstYI     BtgI     BtsI   DraIII     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KasI     MmeI
     MscI     MslI   MspA1I     NarI     NcoI     NheI     PasI    PflMI
     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI
    SanDI     SbfI    SexAI     SfcI     SfiI     SfoI     SmlI     StuI
     StyI    TaqII     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI
    BciVI     BclI    BfrBI    BsiWI    BspHI    BsrGI   BssHII    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     NaeI     NdeI   NgoMIV     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI     ScaI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI     XbaI     XhoI     XmaI     XmnI     ZraI
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