Restriction map for pF1KA0196
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AflII       
AgeI        AhdI        Alw44I      ApaI        ApaLI       
AscI        Asp718I     AspEI       AspHI       AviII       
AvrII       BanII       BbeI        BbrPI       BbsI        
BcgI        BfrI        BglI        BlnI        BpuAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiHKAI     
BsiWI       BsmBI       BspDI       BspEI       BsrFI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
EcoNI       EcoO65I     EcoT22I     FseI        FspI        
HgaI        Hin1I       HpaI        HphI        KasI        
KpnI        KspI        MamI        MluI        MroI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       PvuI        
RsrII       SacI        SacII       SapI        SbfI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    StuI        SwaI        XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccII        2149  CGCG                 CG^CG
AclI         2454  AACGTT               AA^CGTT
AocI           45  CCTAAGG              CC^T[ACGT]AGG
Aor51HI      3101  AGCGCT               AGC^GCT
AseI         1798  ATTAAT               AT^TAAT
AsnI         1798  ATTAAT               AT^TAAT
AspI         2278  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI        1980  GCTGAGG              CCTCAGC(-5/-2)
BmyI         3371  GTGCCC               G[AGT]GC[ACT]^C
BsaAI         775  TACGTG               [CT]AC^GT[GA]
BsaI          711  GGTCTC               GGTCTC(1/5)
BsiEI        3200  CGACCG               CG[GA][CT]^CG
Bsp1286I     3371  GTGCCC               G[AGT]GC[ACT]^C
BspHI        2735  TCATGA               T^CATGA
BspMI        1820  ACCTGC               ACCTGC(4/8)
BsrBI        1342  GAGCGG               CCGCTC(-3/-3)
BstAPI        928  GCAAAAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         569  TTCGAA               TT^CGAA
BstUI        2149  CGCG                 CG^CG
Bsu36I         45  CCTAAGG              CC^T[ACGT]AGG
Eco47III     3101  AGCGCT               AGC^GCT
Eco81I         45  CCTAAGG              CC^T[ACGT]AGG
EcoRI        1528  GAATTC               G^AATTC
MfeI         3119  CAATTG               C^AATTG
MunI         3119  CAATTG               C^AATTG
MvnI         2149  CGCG                 CG^CG
NspV          569  TTCGAA               TT^CGAA
PflFI        2278  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        1798  ATTAAT               AT^TAAT
Psp1406I     2454  AACGTT               AA^CGTT
RcaI         2735  TCATGA               T^CATGA
ScaI         1838  AGTACT               AGT^ACT
SfuI          569  TTCGAA               TT^CGAA
SphI         1011  GCATGC               GCATG^C
SspI         1446  AATATT               AAT^ATT
Tth111I      2278  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          336  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflIII       2509  ACATGT               A^C[GA][CT]GT
             3435  ACATGT              
BamHI        1223  GGATCC               G^GATCC
             2100  GGATCC              
BanI          598  GGTGCC               G^G[CT][GA]CC
             3370  GGTGCC              
BcnI         3207  CCCGG                CC^[GC]GG
             3266  CCCGG               
BglII         688  AGATCT               A^GATCT
             1433  AGATCT              
BseRI        1054  GAGGAG               GAGGAG(10/8)
             1624  GAGGAG              
BsgI         1250  CTGCAC               GTGCAG(16/14)
             1021  GTGCAG              
BsmI         2739  GAATGC               GAATGC(1/-1)
             3062  GCATTC              
BspLU11I     2509  ACATGT               A^CATGT
             3435  ACATGT              
BstXI         190  CCAGAATTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              853  CCAGATAATTGG        
DraII        1655  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2987  AGGACCC             
EcoO109I     1655  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2987  AGGACCC             
EcoRV         157  GATATC               GAT^ATC
              332  GATATC              
FauI         1961  CCCGC                CCCGC(4/6)
             1480  GCGGG               
HaeII        3101  AGCGCT               [GA]GCGC^[CT]
             3288  GGCGCT              
NciI         3207  CCCGG                CC^[GC]GG
             3266  CCCGG               
NcoI         1730  CCATGG               C^CATGG
             2240  CCATGG              
PaeR7I       2189  CTCGAG               C^TCGAG
             2833  CTCGAG              
PflMI        1115  CCATCCGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1533  CCACCAGTTGG         
PpuMI        1655  GGGACCT              [GA]G^G[AT]CC[CT]
             2987  AGGACCC             
PstI         2472  CTGCAG               CTGCA^G
             3356  CTGCAG              
SalI          680  GTCGAC               G^TCGAC
             2465  GTCGAC              
SexAI         594  ACCAGGT              A^CC[AT]GGT
              707  ACCAGGT             
Van91I       1115  CCATCCGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1533  CCACCAGTTGG         
XhoI         2189  CTCGAG               C^TCGAG
             2833  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AvaI        
AvaII       BalI        BbvI        BciVI       BclI        
BfaI        BlpI        BpmI        Bpu1102I    BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1407I    BsrDI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DsaI        EaeI        EarI        EcoRII      EcoT14I     
FbaI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaII       ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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