Restriction map for pF1KA0196
 (Linear) (Six-base) MAPSORT of: KIAA0196.seq  Check: 5896  from: 1  to: 3477



KIAA0196.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:54  ..

AccI GT'mk_AC

Cuts at:      0     320     681    2466    3477 
   Size:        320     361    1785    1011

  Fragments arranged by size: 

                1785    1011     361     320
 
AclI AA'CG_TT

Cuts at:      0    2455    3477 
   Size:       2455    1022
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1915    2072    2320    3271    3449    3477 
   Size:       1915     157     248     951     178      28

  Fragments arranged by size: 

                1915     951     248     178     157      28
 
AfeI AGC'GCT

Cuts at:      0    3103    3477 
   Size:       3103     374
 
AflIII A'CryG_T

Cuts at:      0    2509    3435    3477 
   Size:       2509     926      42

  Fragments arranged by size: 

                2509     926      42
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1239    2115    2868    3285    3477 
   Size:       1239     876     753     417     192

  Fragments arranged by size: 

                1239     876     753     417     192
 
ApoI r'AATT_y

Cuts at:      0     247    1528    2667    2772    2783    3350    3477 
   Size:        247    1281    1139     105      11     567     127

  Fragments arranged by size: 

                1281    1139     567     247     127     105      11
 
AseI AT'TA_AT

Cuts at:      0    1799    3477 
   Size:       1799    1678
 
AvaI C'yCGr_G

Cuts at:      0     622    2189    2833    3477 
   Size:        622    1567     644     644

  Fragments arranged by size: 

                1567     644     644     622
 
BamHI G'GATC_C

Cuts at:      0    1223    2100    3477 
   Size:       1223     877    1377

  Fragments arranged by size: 

                1377    1223     877
 
BanI G'GyrC_C

Cuts at:      0     598    3370    3477 
   Size:        598    2772     107

  Fragments arranged by size: 

                2772     598     107
 
BbvCI CC'TCA_GC

Cuts at:      0    1981    3477 
   Size:       1981    1496
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     731    1239    1869    3477 
   Size:        731     508     630    1608

  Fragments arranged by size: 

                1608     731     630     508
 
BclI T'GATC_A

Cuts at:      0     138    1601    1634    3477 
   Size:        138    1463      33    1843

  Fragments arranged by size: 

                1843    1463     138      33
 
BglII A'GATC_T

Cuts at:      0     688    1433    3477 
   Size:        688     745    2044

  Fragments arranged by size: 

                2044     745     688
 
BlpI GC'TnA_GC

Cuts at:      0     218    1825    1994    3477 
   Size:        218    1607     169    1483

  Fragments arranged by size: 

                1607    1483     218     169
 
Bme1580I G_kGCm'C

Cuts at:      0    3375    3477 
   Size:       3375     102
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1868    2959    3070    3406    3477 
   Size:       1868    1091     111     336      71

  Fragments arranged by size: 

                1868    1091     336     111      71
 
Bpu10I CC'TnA_GC

Cuts at:      0    1981    3477 
   Size:       1981    1496
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1001    3477 
   Size:       1001    2476
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     717    3477 
   Size:        717    2760
 
BsaAI yAC'GTr

Cuts at:      0     777    3477 
   Size:        777    2700
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1841    1871    2338    2368    3477 
   Size:       1841      30     467      30    1109

  Fragments arranged by size: 

                1841    1109     467      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1069    1639    3477 
   Size:       1069     570    1838

  Fragments arranged by size: 

                1838    1069     570
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1042    1235    3477 
   Size:       1042     193    2242

  Fragments arranged by size: 

                2242    1042     193
 
BsiEI CG_ry'CG

Cuts at:      0    3203    3477 
   Size:       3203     274
 
BsmI GAATG_Cn'

Cuts at:      0    2745    3062    3477 
   Size:       2745     317     415

  Fragments arranged by size: 

                2745     415     317
 
Bsp1286I G_dGCh'C

Cuts at:      0    3375    3477 
   Size:       3375     102
 
BspHI T'CATG_A

Cuts at:      0    2735    3477 
   Size:       2735     742
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1829    3477 
   Size:       1829    1648
 
BsrBI CCG'CTC

Cuts at:      0    1344    3477 
   Size:       1344    2133
 
BsrDI GCAATG_nn'

Cuts at:      0      74    1113    2798    3477 
   Size:         74    1039    1685     679

  Fragments arranged by size: 

                1685    1039     679      74
 
BsrGI T'GTAC_A

Cuts at:      0     303    1494    3328    3477 
   Size:        303    1191    1834     149

  Fragments arranged by size: 

                1834    1191     303     149
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     934    3477 
   Size:        934    2543
 
BstBI TT'CG_AA

Cuts at:      0     570    3477 
   Size:        570    2907
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     197     860    3477 
   Size:        197     663    2617

  Fragments arranged by size: 

                2617     663     197
 
BstYI r'GATC_y

Cuts at:      0     688    1223    1433    2100    3477 
   Size:        688     535     210     667    1377

  Fragments arranged by size: 

                1377     688     667     535     210
 
Bsu36I CC'TnA_GG

Cuts at:      0      46    3477 
   Size:         46    3431
 
BtgI C'CryG_G

Cuts at:      0    1730    2240    3314    3477 
   Size:       1730     510    1074     163

  Fragments arranged by size: 

                1730    1074     510     163
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     721    3477 
   Size:        721    2756
 
BtsI GCAGTG_nn'

Cuts at:      0    3331    3477 
   Size:       3331     146
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      12      47    3477 
   Size:         12      35    3430

  Fragments arranged by size: 

                3430      35      12
 
DraI TTT'AAA

Cuts at:      0     941    1457    2726    3477 
   Size:        941     516    1269     751

  Fragments arranged by size: 

                1269     941     751     516
 
EaeI y'GGCC_r

Cuts at:      0      33    3217    3297    3477 
   Size:         33    3184      80     180

  Fragments arranged by size: 

                3184     180      80      33
 
EarI CTCTTCn'nnn_

Cuts at:      0    1234    1511    1916    2584    3477 
   Size:       1234     277     405     668     893

  Fragments arranged by size: 

                1234     893     668     405     277
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2784    3477 
   Size:       2784     693
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1868    1915    2072    2320    2959    3070    3271    3406 
   Size:       1868      47     157     248     639     111     201     135

Cuts at:   3406    3449    3477 
   Size:         43      28

  Fragments arranged by size: 

                1868     639     248     201     157     135     111      47
                  43      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1656    2988    3477 
   Size:       1656    1332     489

  Fragments arranged by size: 

                1656    1332     489
 
EcoRI G'AATT_C

Cuts at:      0    1528    3477 
   Size:       1528    1949
 
EcoRV GAT'ATC

Cuts at:      0     159     334    3477 
   Size:        159     175    3143

  Fragments arranged by size: 

                3143     175     159
 
HaeII r_GCGC'y

Cuts at:      0    3105    3292    3477 
   Size:       3105     187     185

  Fragments arranged by size: 

                3105     187     185
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     632     664    1843    1875    2814    2846    3477 
   Size:        632      32    1179      32     939      32     631

  Fragments arranged by size: 

                1179     939     632     631      32      32      32
 
HincII GTy'rAC

Cuts at:      0     682    2286    2467    3477 
   Size:        682    1604     181    1010

  Fragments arranged by size: 

                1604    1010     682     181
 
HindIII A'AGCT_T

Cuts at:      0    1081    1418    1508    1762    1927    2023    3477 
   Size:       1081     337      90     254     165      96    1454

  Fragments arranged by size: 

                1454    1081     337     254     165      96      90
 
MfeI C'AATT_G

Cuts at:      0    3119    3477 
   Size:       3119     358
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     638    1223    1520    3477 
   Size:        638     585     297    1957

  Fragments arranged by size: 

                1957     638     585     297
 
MscI TGG'CCA

Cuts at:      0      35    3219    3299    3477 
   Size:         35    3184      80     178

  Fragments arranged by size: 

                3184     178      80      35
 
MslI CAynn'nnrTG

Cuts at:      0     667    2062    2095    3477 
   Size:        667    1395      33    1382

  Fragments arranged by size: 

                1395    1382     667      33
 
MspA1I CmG'CkG

Cuts at:      0    1236    2526    2932    3058    3477 
   Size:       1236    1290     406     126     419

  Fragments arranged by size: 

                1290    1236     419     406     126
 
NcoI C'CATG_G

Cuts at:      0    1730    2240    3477 
   Size:       1730     510    1237

  Fragments arranged by size: 

                1730    1237     510
 
NspI r_CATG'y

Cuts at:      0    1015    1902    2400    2513    3439    3477 
   Size:       1015     887     498     113     926      38

  Fragments arranged by size: 

                1015     926     887     498     113      38
 
PciI A'CATG_T

Cuts at:      0    2509    3435    3477 
   Size:       2509     926      42

  Fragments arranged by size: 

                2509     926      42
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1121    1539    3477 
   Size:       1121     418    1938

  Fragments arranged by size: 

                1938    1121     418
 
PfoI T'CCnGG_A

Cuts at:      0    3383    3477 
   Size:       3383      94
 
PpuMI rG'GwC_Cy

Cuts at:      0    1656    2988    3477 
   Size:       1656    1332     489

  Fragments arranged by size: 

                1656    1332     489
 
PspXI vC'TCGA_Gb

Cuts at:      0    2189    3477 
   Size:       2189    1288
 
PstI C_TGCA'G

Cuts at:      0    2476    3360    3477 
   Size:       2476     884     117

  Fragments arranged by size: 

                2476     884     117
 
PvuII CAG'CTG

Cuts at:      0    1236    2526    2932    3058    3477 
   Size:       1236    1290     406     126     419

  Fragments arranged by size: 

                1290    1236     419     406     126
 
SalI G'TCGA_C

Cuts at:      0     680    2465    3477 
   Size:        680    1785    1012

  Fragments arranged by size: 

                1785    1012     680
 
ScaI AGT'ACT

Cuts at:      0    1840    3477 
   Size:       1840    1637
 
SexAI A'CCwGG_T

Cuts at:      0     594     707    3477 
   Size:        594     113    2770

  Fragments arranged by size: 

                2770     594     113
 
SfcI C'TryA_G

Cuts at:      0     229    2472    3356    3477 
   Size:        229    2243     884     121

  Fragments arranged by size: 

                2243     884     229     121
 
SmlI C'TyrA_G

Cuts at:      0    1016    2189    2833    3477 
   Size:       1016    1173     644     644

  Fragments arranged by size: 

                1173    1016     644     644
 
SphI G_CATG'C

Cuts at:      0    1015    3477 
   Size:       1015    2462
 
SspI AAT'ATT

Cuts at:      0    1448    3477 
   Size:       1448    2029
 
StyI C'CwwG_G

Cuts at:      0    1730    2240    2600    3477 
   Size:       1730     510     360     877

  Fragments arranged by size: 

                1730     877     510     360
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     826    3217    3358    3477 
   Size:        826    2391     141     119

  Fragments arranged by size: 

                2391     826     141     119
 
TatI w'GTAC_w

Cuts at:      0     303     428     574    1494    1838    3328    3477 
   Size:        303     125     146     920     344    1490     149

  Fragments arranged by size: 

                1490     920     344     303     149     146     125
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2338    2370    2400    2432    3212    3244    3477 
   Size:       2338      32      30      32     780      32     233

  Fragments arranged by size: 

                2338     780     233      32      32      32      30
 
Tth111I GACn'n_nGTC

Cuts at:      0    2281    3477 
   Size:       2281    1196
 
XbaI T'CTAG_A

Cuts at:      0     336    3477 
   Size:        336    3141
 
XhoI C'TCGA_G

Cuts at:      0    2189    2833    3477 
   Size:       2189     644     644

  Fragments arranged by size: 

                2189     644     644
 
XmnI GAAnn'nnTTC

Cuts at:      0     248    1412    1520    3256    3477 
   Size:        248    1164     108    1736     221

  Fragments arranged by size: 

                1736    1164     248     221     108
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AfeI   AflIII    AlwNI     ApoI     AseI
     AvaI    BamHI     BanI    BbvCI    BciVI     BclI    BglII     BlpI
 Bme1580I     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI    BseRI
     BsgI    BsiEI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI     EaeI     EarI     EciI  Eco57MI EcoO109I
    EcoRI    EcoRV    HaeII    Hin4I   HincII  HindIII     MfeI     MmeI
     MscI     MslI   MspA1I     NcoI     NspI     PciI    PflMI     PfoI
    PpuMI    PspXI     PstI    PvuII     SalI     ScaI    SexAI     SfcI
     SmlI     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BanII
     BbeI     BbsI     BcgI    BfrBI     BglI    BmgBI     BmrI     BmtI
     BplI    BsaBI    BsaHI    BsaWI    BseYI  BsiHKAI    BsiWI    BsmBI
    BspEI    BsrFI   BssHII    BssSI   BstEII  BstZ17I     ClaI   DraIII
     DrdI     EagI  EcoICRI    EcoNI     FalI     FseI     FspI    FspAI
     HpaI     KasI     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI     PsrI     PvuI    RsrII     SacI
    SacII    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI     XcmI     XmaI
     ZraI
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