Restriction map for pF1KA0188
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BbeI        BcgI        BfaI        
BfrI        BglII       BlnI        BsiEI       BsiWI       
Bsp1407I    BsrBI       BsrGI       Bst1107I    BstBI       
BstZ17I     CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRI       EcoRV       
FseI        FspI        HpaI        KasI        KpnI        
KspI        MaeI        MfeI        MluI        MunI        
NarI        NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacII       SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII       1261  TCCGGA               T^CCGGA
AflIII        539  ACATGT               A^C[GA][CT]GT
ApaI         2248  GGGCCC               GGGCC^C
Asp700I      2564  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        1215  GGATCC               G^GATCC
BanI         1987  GGCACC               G^G[CT][GA]CC
BbrPI        1971  CACGTG               CAC^GTG
BciVI        2592  GGATAC               GTATCC(6/5)
BclI          282  TGATCA               T^GATCA
BglI         2241  GCCCCAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         277  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1045  GAGACC               GGTCTC(1/5)
BseAI        1261  TCCGGA               T^CCGGA
BsmBI         430  GAGACG               CGTCTC(1/5)
BspDI        1498  ATCGAT               AT^CGAT
BspEI        1261  TCCGGA               T^CCGGA
BspHI        1622  TCATGA               T^CATGA
BspLU11I      539  ACATGT               A^CATGT
BspMI        2207  ACCTGC               ACCTGC(4/8)
BsrDI        1573  GCAATG               GCAATG(2/0)
BsrFI          22  GCCGGC               [GA]^CCGG[CT]
BssHII       1812  GCGCGC               G^CGCGC
BssSI        1513  CTCGTG               CACGAG(-5/-1)
BstAPI       1270  GCAAAACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        707  GGTCACC              G^GT[ACGT]ACC
BstPI         707  GGTCACC              G^GT[ACGT]ACC
Cfr10I         22  GCCGGC               [GA]^CCGG[CT]
ClaI         1498  ATCGAT               AT^CGAT
DraI         2323  TTTAAA               TTT^AAA
DrdI           94  GACATCATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        2085  CCTTGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       707  GGTCACC              G^GT[ACGT]ACC
EcoT22I      1442  ATGCAT               ATGCA^T
FbaI          282  TGATCA               T^GATCA
HaeII        1062  AGCGCC               [GA]GCGC^[CT]
HindIII      1908  AAGCTT               A^AGCTT
MamI          277  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1261  TCCGGA               T^CCGGA
NaeI           22  GCCGGC               GCC^GGC
NcoI          572  CCATGG               C^CATGG
NdeI          229  CATATG               CA^TATG
NgoMIV         22  GCCGGC               G^CCGGC
NsiI         1442  ATGCAT               ATGCA^T
PmaCI        1971  CACGTG               CAC^GTG
PmlI         1971  CACGTG               CAC^GTG
RcaI         1622  TCATGA               T^CATGA
SalI         2536  GTCGAC               G^TCGAC
SfcI          449  CTGTAG               C^T[GA][CT]AG
XmnI         2564  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccI         1189  GTCTAC               GT^[AC][GT]AC
             2536  GTCGAC              
AccII        1813  CGCG                 CG^CG
             2198  CGCG                
AcsI          899  AAATTT               [GA]^AATT[CT]
             2152  AAATTT              
AcyI          432  GACGCC               G[GA]^CG[CT]C
             1186  GGCGTC              
AocI          637  CCTGAGG              CC^T[ACGT]AGG
             1049  CCTTAGG             
ApoI          899  AAATTT               [GA]^AATT[CT]
             2152  AAATTT              
AspI          432  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1183  GACGGCGTC           
AvaI          179  CCCGAG               C^[CT]CG[GA]G
             1332  CTCGGG              
BcnI         1295  CCCGG                CC^[GC]GG
             1425  CCGGG               
BlpI         1761  GCTCAGC              GC^T[ACGT]AGC
             2116  GCTAAGC             
Bpu1102I     1761  GCTCAGC              GC^T[ACGT]AGC
             2116  GCTAAGC             
BsaAI           7  TACGTG               [CT]AC^GT[GA]
             1971  CACGTG              
BsaHI         432  GACGCC               G[GA]^CG[CT]C
             1186  GGCGTC              
BsgI         2209  CTGCAC               GTGCAG(16/14)
             2287  CTGCAC              
BsmI          349  GAATGC               GAATGC(1/-1)
             1444  GCATTC              
BsoBI         179  CCCGAG               C^[CT]CG[GA]G
             1332  CTCGGG              
BstUI        1813  CGCG                 CG^CG
             2198  CGCG                
BstYI        1215  GGATCC               [GA]^GATC[CT]
             1254  AGATCC              
Bsu36I        637  CCTGAGG              CC^T[ACGT]AGG
             1049  CCTTAGG             
CelII        1761  GCTCAGC              GC^T[ACGT]AGC
             2116  GCTAAGC             
DsaI          572  CCATGG               C^C[GA][CT]GG
              779  CCACGG              
Eco81I        637  CCTGAGG              CC^T[ACGT]AGG
             1049  CCTTAGG             
Hin1I         432  GACGCC               G[GA]^CG[CT]C
             1186  GGCGTC              
MflI         1215  GGATCC               [GA]^GATC[CT]
             1254  AGATCC              
MseI         1904  TTAA                 T^TAA
             2324  TTAA                
MvnI         1813  CGCG                 CG^CG
             2198  CGCG                
NciI         1295  CCCGG                CC^[GC]GG
             1425  CCGGG               
PflFI         432  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1183  GACGGCGTC           
PpuMI         167  GGGTCCT              [GA]G^G[AT]CC[CT]
             1376  GGGACCT             
SfaNI        1441  GATGC                GCATC(5/9)
             2111  GCATC               
SspI         1535  AATATT               AAT^ATT
             2451  AATATT              
StuI          387  AGGCCT               AGG^CCT
             1580  AGGCCT              
Tru9I        1904  TTAA                 T^TAA
             2324  TTAA                
Tth111I       432  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1183  GACGGCGTC           
XhoII        1215  GGATCC               [GA]^GATC[CT]
             1254  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BalI        BanII       BbsI        BbvCI       
BbvI        BmyI        BpmI        BpuAI       BsaJI       
BsaWI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstXI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        
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